Essential Ion Channel Methods

Author : P. Michael Conn
Publisher : Academic Press
Page : 629 pages
File Size : 48,7 Mb
Release : 2010-07-22
Category : Medical
ISBN : 9780123822376

Get Book

Essential Ion Channel Methods by P. Michael Conn Pdf

The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels. As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, and provide tricks and approaches that make it possible to adapt methods to other systems. Methods are presented in a fashion that allows their replication by individuals new to the field, yet providing valuable information for seasoned investigators. Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with graphics and easy to follow methods Loaded with detailed protocols developed and used by leaders in the field Refines, organizes and updates popular methods from one of our top selling series, Methods in Enzymology

Author : Nikita Gamper
Publisher : Humana Press
Page : 456 pages
File Size : 47,8 Mb
Release : 2016-05-01
Category : Science
ISBN : 1493960091

Get Book

Ion Channels by Nikita Gamper Pdf

This Methods in Molecular Biology book offers strategies and protocols for studying a large group of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Includes step-by-step protocols, materials lists, tips and more."

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 331 pages
File Size : 43,8 Mb
Release : 2023-06-09
Category : Medical
ISBN : 9781000857757

Get Book

Textbook of Ion Channels Volume I by Jie Zheng,Matthew C. Trudeau Pdf

The Textbook of Ion Channels is a set of three volumes providing a wide-ranging reference source on ion channels for students, instructors and researchers. Ion channels are membrane proteins that control the electrical properties of neurons and cardiac cells; mediate the detection and response to sensory stimuli like light, sound, odor, and taste; and regulate the response to physical stimuli like temperature and pressure. In non-excitable tissues, ion channels are instrumental for the regulation of basic salt balance that is critical for homeostasis. Ion channels are located at the surface membrane of cells, giving them the unique ability to communicate with the environment, as well as the membrane of intracellular organelles, allowing them to regulate internal homeostasis. Ion channels are fundamentally important for human health and diseases, and are important targets for pharmaceuticals in mental illness, heart disease, anesthesia, pain and other clinical applications. The modern methods used in their study are powerful and diverse, ranging from single ion-channel measurement techniques to models of ion channel diseases in animals, and human clinical trials for ion channel drugs. Volume I, Part 1 covers fundamental topics such as the basic principles of ion permeation and selectivity, voltage-dependent, ligand-dependent, and mechano-dependent ion channel activation mechanisms, the mechanisms for ion channel desensitization and inactivation, and basic ion channel pharmacology and inhibition. Volume I, Part 2 offers a practical guide of cardinal methods for researching ion channels, including heterologous expression and voltage-clamp and patch-clamp electrophysiology; isolation of native currents using patch clamping; modeling ion channel gating, structures, and its dynamics; crystallography and cryo-electron microscopy; fluorescence and paramagnetic resonance spectroscopy methods; and genetics approaches in model organisms. All three volumes give the reader an introduction to fundamental concepts needed to understand the mechanism of ion channels; a guide to the technical aspects of ion channel research; a modern guide to the properties of major ion channel families; and includes coverage of key examples of regulatory, physiological and disease roles for ion channels.

Author : Nikita Gamper
Publisher : Humana Press
Page : 440 pages
File Size : 50,5 Mb
Release : 2013-03-26
Category : Science
ISBN : 1627033521

Get Book

Ion Channels by Nikita Gamper Pdf

Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie basic cellular functions such as communication, contractility, and metabolism. In Ion Channels: Methods and Protocols, Second Edition, experts in the field contribute chapters that focus on the strategies, approaches, methods, and protocols for studying a large family of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Using practical examples from the cutting-edge current research, this volume will take a look back at the major methods and approaches that aided the progress toward the current understanding of ion channel function, structural design, and biological roles. The volume also aims to look forward and identify approaches that will lead us to future discoveries. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 246 pages
File Size : 49,9 Mb
Release : 2023-06-28
Category : Medical
ISBN : 9781000857849

Get Book

Textbook of Ion Channels Volume III by Jie Zheng,Matthew C. Trudeau Pdf

The Textbook of Ion Channels is a set of three volumes that provides a wide-ranging reference source on ion channels for students, instructors and researchers. Ion channels are mem- brane proteins that control the electrical properties of neurons and cardiac cells; medi- ate the detection and response to sensory stimuli like light, sound, odor, and taste; and regulate the response to physical stimuli like temperature and pressure. In non-excitable tissues, ion channels are instrumental for the regulation of basic salt balance that is critical for homeostasis. Ion channels are located at the surface membrane of cells, giving them the unique ability to communicate with the environment, as well as the membrane of intracellular organelles, allowing them to regulate internal homeostasis. Ion channels are fundamentally important for human health and diseases, and are important targets for pharmaceuticals in mental illness, heart disease, anesthesia, pain and other clinical appli- cations. The modern methods used in their study are powerful and diverse, ranging from single ion-channel measurement techniques to models of ion channel diseases in animals, and human clinical trials for ion channel drugs. Volume III includes coverage of key ion channel regulators and their mechanisms, the role of ion channels working in concert in selected physiological systems, and examples of ion channel mutations and dysfunction in a selection of diseases. Chapters on ion channel regulation include splice variants, calcium–calmodulin regulation, regulation by G pro- teins, and lipids. A selection of ion channels in physiological systems includes ion chan- nels of the heart, ion channels in immune cells and their role in pancreatic beta cells and regulation of insulin secretion, and the role of channels in sperm and eggs. While disease mechanisms are integrated into the chapters of Volume II, Volume III offers special consid- eration of ion channels in epilepsy, cystic fibrosis and pain syndromes. All three volumes give the reader an introduction to fundamental concepts needed to understand the mechanism of ion channels, a guide to the technical aspects of ion channel research, offer a modern guide to the properties of major ion channel families, and includecoverage of key examples of regulatory, physiological, and disease roles for ion channels.

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 682 pages
File Size : 46,9 Mb
Release : 2015-02-25
Category : Medical
ISBN : 9781466551428

Get Book

Handbook of Ion Channels by Jie Zheng,Matthew C. Trudeau Pdf

The New Benchmark for Understanding the Latest Developments of Ion ChannelsIon channels control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli, and regulate the response to physical stimuli. They can often interact with the cellular environment due to their location at the surface of ce

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 48,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

Get Book

Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Rafael Luján,Francisco Ciruela
Publisher : Humana Press
Page : 475 pages
File Size : 50,6 Mb
Release : 2018-03-31
Category : Medical
ISBN : 1493979833

Get Book

Receptor and Ion Channel Detection in the Brain by Rafael Luján,Francisco Ciruela Pdf

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

Author : Frances M. Ashcroft
Publisher : Academic Press
Page : 505 pages
File Size : 49,9 Mb
Release : 1999-10-20
Category : Science
ISBN : 9780080535210

Get Book

Ion Channels and Disease by Frances M. Ashcroft Pdf

Ion channels are membrane proteins that act as gated pathways for the movement of ions across cell membranes. They play essential roles in the physiology of all cells. In recent years, an ever-increasing number of human and animal diseases have been found to result from defects in ion channel function. Most of these diseases arise from mutations in the genes encoding ion channel proteins, and they are now referred to as the channelopathies. Ion Channels and Disease provides an informative and up-to-date account of our present understanding of ion channels and the molecular basis of ion channel diseases. It includes a basic introduction to the relevant aspects of molecular biology and biophysics and a brief description of the principal methods used to study channelopathies. For each channel, the relationship between its molecular structure and its functional properties is discussed and ways in which genetic mutations produce the disease phenotype are considered. This book is intended for research workers and clinicians, as well as graduates and advanced undergraduates. The text is clear and lively and assumes little knowledge, yet it takes the reader to frontiers of what is currently known about this most exciting and medically important area of physiology. Introduces the relevant aspects of molecular biology and biophysics Describes the principal methods used to study channelopathies Considers single classes of ion channels with summaries of the physiological role, subunit composition, molecular structure and chromosomal location, plus the relationship between channel structure and function Looks at those diseases associated with defective channel structures and regulation, including mutations affecting channel function and to what extent this change in channel function can account for the clinical phenotype

Author : Satya Prakash Gupta
Publisher : Springer Science & Business Media
Page : 358 pages
File Size : 52,8 Mb
Release : 2011-06-21
Category : Science
ISBN : 9783642199226

Get Book

Ion Channels and Their Inhibitors by Satya Prakash Gupta Pdf

Being the crucial components of living cells, ion channels are important targets of therapeutic agents. Historically, it has been challenging to develop drugs on this target class. A major issue with target based ion channel drug development is the identification of effective small chemical leads for medicinal chemistry optimization to the clinical candidate status. Thus enough attention has been paid to the study of structure and functions of ion channels and their potential inhibitors. The present book compiles important chapters authored by eminent workers in the field to cover important recent advances in the studies of the structure and functions of ion channels and their inhibitors, such as sodium ion, potassium ion, chloride ion, calcium ion channel inhibitors. The book may be of great use to the students and scientists working in the area of molecular biology, biochemistry, physiology, neurobiology, and medicinal chemistry.

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 1067 pages
File Size : 53,7 Mb
Release : 2023-07-06
Category : Medical
ISBN : 9781003820239

Get Book

Textbook of Ion Channels by Jie Zheng,Matthew C. Trudeau Pdf

The Textbook of Ion Channels is a set of three volumes providing a wide-ranging reference source on ion channels for students, instructors, and researchers. Ion channels are membrane proteins that control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli like light, sound, odor, and taste, and regulate the response to physical stimuli like temperature and pressure. In non-excitable tissues, ion channels are instrumental for the regulation of basic salt balance that is critical for homeostasis. Ion channels are located at the surface membrane of cells, giving them the unique ability to communicate with the environment, as well as the membrane of intracellular organelles, allowing them to regulate internal homeostasis. Ion channels are fundamentally important for human health and diseases, and are important targets for pharmaceuticals in mental illness, heart disease, anesthesia, pain and other clinical applications. The modern methods used in their study are powerful and diverse, ranging from single ion-channel measurement techniques to models of ion channel diseases in animals, and human clinical trials for ion channel drugs. All three volumes give the reader an introduction to fundamental concepts needed to understand the mechanism of ion channels, a guide to the technical aspects of ion channel research, offer a modern guide to the properties of major ion channel families, and include coverage of key examples of regulatory, physiological, and disease roles for ion channels.

Author : Jeffrey J. Clare,Derek J. Trezise
Publisher : John Wiley & Sons
Page : 302 pages
File Size : 40,8 Mb
Release : 2006-08-21
Category : Science
ISBN : 9783527607938

Get Book

Expression and Analysis of Recombinant Ion Channels by Jeffrey J. Clare,Derek J. Trezise Pdf

Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 691 pages
File Size : 40,9 Mb
Release : 2015-02-25
Category : Medical
ISBN : 1466551402

Get Book

Handbook of Ion Channels by Jie Zheng,Matthew C. Trudeau Pdf

The New Benchmark for Understanding the Latest Developments of Ion Channels Ion channels control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli, and regulate the response to physical stimuli. They can often interact with the cellular environment due to their location at the surface of cells. In nonexcitable tissues, they also help regulate basic salt balance critical for homeostasis. All of these features make ion channels important targets for pharmaceuticals. Handbook of Ion Channels illustrates the fundamental importance of these membrane proteins to human health and disease. Renowned researchers from around the world introduce the technical aspects of ion channel research, provide a modern guide to the properties of major ion channels, and present powerful methods for modeling ion channel diseases and performing clinical trials for ion channel drugs. Conveniently divided into five parts, the handbook first describes the basic concepts of permeation and gating mechanisms, balancing classic theories and the latest developments. The second part covers the principles and practical issues of both traditional and new ion channel techniques and their applications to channel research. The third part organizes the material to follow the superfamilies of ion channels. This part focuses on the classification, properties, gating mechanisms, function, and pharmacology of established and novel channel types. The fourth part addresses ion channel regulation as well as trafficking and distribution. The final part examines several ion channel-related diseases, discussing genetics, mechanisms, and pharmaceutical advances.

Author : Xu Tian-le,Long-Jun Wu
Publisher : CRC Press
Page : 341 pages
File Size : 42,9 Mb
Release : 2021-08-05
Category : Science
ISBN : 9781000403749

Get Book

Nonclassical Ion Channels in the Nervous System by Xu Tian-le,Long-Jun Wu Pdf

Ion channels generate bioelectricity. Recent findings have documented the biophysical properties, the structure, assembly and regulation, and function and dysfunction of nonclassical nervous system ion channels. This book reviews nonclassical ion channel research, ranging from the basic biology, structure, regulations to their functions not only in normal physiology but also neurological disorders, using a variety of cutting-edge techniques and novel animal models.

Author : Jie Zheng,Matthew C. Trudeau
Publisher : Unknown
Page : 0 pages
File Size : 49,8 Mb
Release : 2023
Category : MEDICAL
ISBN : 1003096212

Get Book

Textbook of Ion Channels: Properties, function, and pharmacology of the superfamilies by Jie Zheng,Matthew C. Trudeau Pdf

"From the editors of the renowned Handbook of Ion Channels, Textbook of Ion Channels is a student-friendly, advanced textbook that illustrates the fundamental importance of these membrane proteins to human health and disease. It has been split into three separate volumes so that each volume can target different needs. The basic concepts of ion channel research are introduced in the first volume, whilst volume two focuses on the pharmacology of ion channels' superfamilies, and volume three presents powerful methods for modeling ion channel diseases and performing clinical trials for ion channel drugs"--