Flow Cytometry In Hematopathology

Author : Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan
Publisher : Springer Science & Business Media
Page : 220 pages
File Size : 48,9 Mb
Release : 2002-11-26
Category : Medical
ISBN : 9781592593545

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Flow Cytometry in Hematopathology by Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan Pdf

Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature.

Author : Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan
Publisher : Springer Science & Business Media
Page : 344 pages
File Size : 40,9 Mb
Release : 2008-03-04
Category : Medical
ISBN : 9781597451628

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Flow Cytometry in Hematopathology by Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan Pdf

The second edition of this volume reflects the recent advances in the FCM analysis of hematopoietic disorders. The chapters have been revised to incorporate new text and figures. The volume is aimed at hematopathologists, hematologists, pathologists, and laboratory technicians.

Author : Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan
Publisher : Unknown
Page : 236 pages
File Size : 50,7 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 1475752393

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Flow Cytometry in Hematopathology by Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan Pdf

Author : Claudio Ortolani
Publisher : John Wiley & Sons
Page : 464 pages
File Size : 47,8 Mb
Release : 2021-04-19
Category : Medical
ISBN : 9781119611271

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Flow Cytometry of Hematological Malignancies by Claudio Ortolani Pdf

Flow Cytometry of Hematological Malignancies Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes: Explorations of more than 70 antigens Full-color illustrations throughout New descriptions of recently discovered markers WHO classifications of hematological neoplastic diseases Helpful tips for result interpretation and analysis Featuring all this and more, Flow Cytometry of Hematological Malignancies, Second Edition, is an invaluable resource for both trainee and experienced hematologists, hematopathologists, oncologists, and pathologists, as well as medical students and diagnostic lab technicians.

Author : Mike Leach,Mark Drummond,Allyson Doig
Publisher : John Wiley & Sons
Page : 0 pages
File Size : 50,8 Mb
Release : 2013-04-22
Category : Medical
ISBN : 0470671203

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Practical Flow Cytometry in Haematology Diagnosis by Mike Leach,Mark Drummond,Allyson Doig Pdf

The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

Author : Mike Leach,Mark Drummond,Allyson Doig,Pam McKay,Bob Jackson,Barbara J. Bain
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 42,8 Mb
Release : 2015-06-01
Category : Medical
ISBN : 9781118746899

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Practical Flow Cytometry in Haematology by Mike Leach,Mark Drummond,Allyson Doig,Pam McKay,Bob Jackson,Barbara J. Bain Pdf

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Author : Wojciech Gorczyca
Publisher : CRC Press
Page : 1167 pages
File Size : 41,7 Mb
Release : 2017-07-06
Category : Medical
ISBN : 9781498775045

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Flow Cytometry in Neoplastic Hematology by Wojciech Gorczyca Pdf

This third edition is the product of the author’s 25 years of experience with flow cytometry; although it covers the wide spectrum of hematopoietic tumors, the focus remains on most important clinical diagnoses, such as acute promyelocytic leukemia, identification of blasts, identification of clonal B-cell population, differentiating mature versus immature T-cell proliferations, deferential diagnosis between hematogones and B-ALL or distinction between chronic and acute monocytic proliferations. All hematopathologists and neoplastic hematologists will find this an important resource for keeping up to date with developments in clinical practice.

Author : Wojciech Gorczyca
Publisher : CRC Press
Page : 350 pages
File Size : 41,5 Mb
Release : 2010-02-25
Category : Medical
ISBN : 9781841847443

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Flow Cytometry in Neoplastic Hematology by Wojciech Gorczyca Pdf

This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.

Author : Sindhu Cherian,Brent Wood
Publisher : Unknown
Page : 165 pages
File Size : 50,9 Mb
Release : 2012
Category : Medical
ISBN : 0983706824

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Flow Cytometry in Evaluation of Hematopoietic Neoplasms by Sindhu Cherian,Brent Wood Pdf

Flow Cytometry in Evaluation of Hematopoietic Neoplasms: A Case-Based Approach is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. Using multi-color techniques pioneered by Brent Wood, the text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing). The publication contains an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem- cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion, enabling readers to develop and work through a differential diagnosis and recognize potential diagnostic pitfalls. Take-home points for each case emphasize critical concepts in flow cytometric diagnosis. Flow Cytometry in Evaluation of Hematopoietic Neoplasms provides pathologists, residents, laboratory technologists, and hematologists with both a study guide and an atlas for regular consultation in the clinical flow cytometry laboratory. The 2008 World Health Organization (WHO) classification system, French-American-British (FAB) classifications, and 2006 Bethesda Consensus recommendations are incorporated in the text.

Author : Anna Porwit,Marie Christine Béné
Publisher : Cambridge University Press
Page : 247 pages
File Size : 54,6 Mb
Release : 2018-01-25
Category : Medical
ISBN : 9781107503830

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Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by Anna Porwit,Marie Christine Béné Pdf

This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.

Author : Ole Didrik Laerum,Ole Didrik Lærum,Robert Bjerknes
Publisher : Unknown
Page : 304 pages
File Size : 41,9 Mb
Release : 1992
Category : Blood
ISBN : UOM:39015029255331

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Flow Cytometry in Hematology by Ole Didrik Laerum,Ole Didrik Lærum,Robert Bjerknes Pdf

This book reviews flow cytometric methods (techniques for measuring and sorting of cells) used in hematology--ranging from those in routine use (such as leukocyte counting and immunophentotyping in diseases like leukemia and AIDS) to those that have potential future use in experimental and clinical hematology. This volume will be of interest to a wide audience, including cell biologists, hematologists, cancer researchers, and HIV/AIDS researchers.

Author : David E. Keren
Publisher : American Society of Clinical Oncology
Page : 384 pages
File Size : 50,6 Mb
Release : 2007
Category : Flow cytometry
ISBN : 0891895485

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Flow Cytometry in Clinical Diagnosis by David E. Keren Pdf

The new edition promises a completely current overview of this increasingly commonplace technology. With every chapter either completely reworked or significantly updated to include the latest developments and new contributions from 10 new international authorities, this will be the go-to resource on clinical flow cytometry for years to come. More than that it promises to deliver a uniquely engaging learning experience with a bonus CD filled with real-world cases and interpretations. Drs. Carey and McCoy bring the new edition of 'Flow Cytometry in Clinical Diagnosis' alive with the bonus CD, which includes data from actual flow analysis and patient samples for 39 cases.

Author : Marion G. Macey
Publisher : Springer Science & Business Media
Page : 294 pages
File Size : 54,9 Mb
Release : 2007-11-03
Category : Medical
ISBN : 9781597454513

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Flow Cytometry by Marion G. Macey Pdf

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Author : Wojciech Gorczyca
Publisher : Informa Healthcare
Page : 266 pages
File Size : 49,9 Mb
Release : 2006
Category : Medical
ISBN : 1841845655

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Flow Cytometry in Neoplastic Hematology by Wojciech Gorczyca Pdf

Key features of this highly illustrated and practical guide include comprehensive coverage of the aspects relevant for clinical diagnosis, an explanation of the general parameters useful in diagnosis with flow cytometry, correlation with color histomorphological findings throughout, and a systematic approach from basic cases to complicated problem areas. All hematopathologists and neoplastic hematologists will find this an important resource for keeping up to date with developments in clinical practice.

Author : Cherie H. Dunphy
Publisher : Amer Society of Clinical
Page : 323 pages
File Size : 40,8 Mb
Release : 2009-11-01
Category : Medical
ISBN : 0891896333

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Integrated Hematopathology by Cherie H. Dunphy Pdf

Unlike similar texts, which are often laden in technological details, Dr. Dunphy offers a comprehensive flow cytometry textbook, which covers in great depth, the technical aspects of FCI, phenotypic markers, as well as the advantages and disadvantages of FCI, but also a quick diagnostic guide incorporating normal and abnormal phenotypic findings of peripheral blood, body fluids, bone marrow, and lymph nodes. Integration of flow cytometry immunophenotyping with morphology and molecular genetic findings provides a complete diagnostic picture. The diagnostic applications of FCI in combination with morphology for all important hematolymphoid disorders. Detailed discussions of FCI mirror the new 2008 WHO classification, to increase the ease of use for practicing hematopathologists and residents. Separate chapters detail unique applications of FCI Brand new: 2008 WHO Classification