Flow Cytometry Of Hematological Malignancies

Author : Claudio Ortolani
Publisher : John Wiley & Sons
Page : 464 pages
File Size : 48,5 Mb
Release : 2021-04-19
Category : Medical
ISBN : 9781119611271

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Flow Cytometry of Hematological Malignancies by Claudio Ortolani Pdf

Flow Cytometry of Hematological Malignancies Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes: Explorations of more than 70 antigens Full-color illustrations throughout New descriptions of recently discovered markers WHO classifications of hematological neoplastic diseases Helpful tips for result interpretation and analysis Featuring all this and more, Flow Cytometry of Hematological Malignancies, Second Edition, is an invaluable resource for both trainee and experienced hematologists, hematopathologists, oncologists, and pathologists, as well as medical students and diagnostic lab technicians.

Author : Anna Porwit,Marie Christine Béné
Publisher : Cambridge University Press
Page : 247 pages
File Size : 49,6 Mb
Release : 2018-01-25
Category : Medical
ISBN : 9781107503830

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Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by Anna Porwit,Marie Christine Béné Pdf

This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.

Author : Tsieh Sun
Publisher : Lippincott Williams & Wilkins
Page : 449 pages
File Size : 55,7 Mb
Release : 2012-01-19
Category : Medical
ISBN : 9781451180640

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Flow Cytometry, Immunohistochemistry, and Molecular Genetics for Hematologic Neoplasms by Tsieh Sun Pdf

Immunophenotyping is the most powerful tool in the routine diagnosis of hematologic neoplasms. Immunohistochemical technique is used in histology labs for this purpose, while flow cytometry is used in clinical labs. Although separately these 2 techniques are very useful in detecting lymphomas and leukemias, the combination of both creates a very powerful and definitive diagnostic tool. The addition of molecular genetics to the book makes it an all-encompassing reference text.

Author : Mike Leach,Mark Drummond,Allyson Doig,Pam McKay,Bob Jackson,Barbara J. Bain
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 46,9 Mb
Release : 2015-06-01
Category : Medical
ISBN : 9781118746899

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Practical Flow Cytometry in Haematology by Mike Leach,Mark Drummond,Allyson Doig,Pam McKay,Bob Jackson,Barbara J. Bain Pdf

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Author : Mike Leach,Mark Drummond,Allyson Doig
Publisher : John Wiley & Sons
Page : 0 pages
File Size : 55,8 Mb
Release : 2013-04-22
Category : Medical
ISBN : 0470671203

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Practical Flow Cytometry in Haematology Diagnosis by Mike Leach,Mark Drummond,Allyson Doig Pdf

The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

Author : Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan
Publisher : Springer Science & Business Media
Page : 220 pages
File Size : 50,5 Mb
Release : 2002-11-26
Category : Medical
ISBN : 9781592593545

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Flow Cytometry in Hematopathology by Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan Pdf

Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature.

Author : Tsieh Sun
Publisher : Lippincott Williams & Wilkins
Page : 362 pages
File Size : 48,6 Mb
Release : 2008
Category : Medical
ISBN : 078178400X

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Flow Cytometry and Immunohistochemistry for Hematologic Neoplasms by Tsieh Sun Pdf

This text is a detailed guide to the use of flow cytometry, immunohistochemistry, and molecular genetic techniques for diagnosis of hematologic neoplasms. Dr. Sun explains the principles of these techniques and demonstrates their utility in 39 clinical cases covering all important entities. Each case represents a comprehensive diagnostic approach including a clinical history and flow cytometric, immunohistochemical, and molecular genetic findings. Abundant full-color illustrations show histologic sections, immunohistochemical stains, bone marrow, peripheral blood, and body fluid smears, and each case includes a complete set of flow cytometric histograms. Over 100 tables compare and differentiate the diagnostic features of similar diseases. An image bank will be available on a companion Website.

Author : Wojciech Gorczyca
Publisher : CRC Press
Page : 350 pages
File Size : 48,5 Mb
Release : 2010-02-25
Category : Medical
ISBN : 9781841847443

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Flow Cytometry in Neoplastic Hematology by Wojciech Gorczyca Pdf

This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.

Author : David M. Dorfman
Publisher : Elsevier Health Sciences
Page : 128 pages
File Size : 44,8 Mb
Release : 2017-11-19
Category : Medical
ISBN : 9780323552837

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Flow Cytometry, An Issue of Clinics in Laboratory Medicine, E-Book by David M. Dorfman Pdf

This issue of Clinics in Laboratory Medicine, edited by David M. Dorfman, will cover Flow Cytometry. Topics covered in this issue include: Flow cytometric evaluation of primary immunodeficiencies; Flow cytometry of B cell neoplasms; Flow cytometry of T cell neoplasms; Flow cytometry of acute myeloid leukemias; AML minimal residual disease (MRD) assessment by flow cytometric analysis; Acute lymphoblastic leukemia minimal residual disease (MRD) assessment by flow cytometric analysis; Flow cytometric assessment of myelodysplasia and myeloproliferative neoplasms; Flow cytometry of plasma cell neoplasms, including MRD testing; Paroxysmal nocturnal hemoglobinuria (PNH) assessment by flow cytometric analysis; Mast cell disease assessment by flow cytometric analysis; Flow cytometry in pediatric hematopathology; Clinical applications of mass cytometry; Automated analysis of clinical flow cytometry data; and Cost-effective flow cytometric testing strategies.

Author : Wendy N. Erber
Publisher : Cambridge University Press
Page : 349 pages
File Size : 44,8 Mb
Release : 2010-11-11
Category : Medical
ISBN : 9781139491730

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Diagnostic Techniques in Hematological Malignancies by Wendy N. Erber Pdf

The diagnosis and monitoring of hematological malignancies is complex and requires a systematic approach. Morphology, cell phenotyping, cytogenetics and molecular genetics are essential, and the results must be integrated. Diagnostic Techniques in Hematological Malignancies details the principles and applications of each of these test types in the diagnosis of hematological malignancies in blood and bone marrow. The first section describes the test modalities – including methodological principles, data interpretation and limitations – and is illustrated by clinical examples. The second section focuses on the clinical entities, detailing the most appropriate tests for diagnosis, staging and monitoring of different hematological malignancies and includes test utilization to identify prognostic markers and potential therapeutic targets. With contributions from multiple international experts, this illustrated book is an essential resource for qualified and trainee hematologists, oncologists, and pathologists. It's a practical and useful guide, providing a rational and structured approach to the laboratory assessment of hematological malignancies.

Author : Tsieh Sun
Publisher : Igaku-Shoin Medical Publishers
Page : 240 pages
File Size : 55,8 Mb
Release : 1993
Category : Flow cytometry
ISBN : UOM:39015029553669

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Color Atlas--text of Flow Cytometric Analysis of Hematologic Neoplasms by Tsieh Sun Pdf

Author : Wojciech Gorczyca
Publisher : CRC Press
Page : 1461 pages
File Size : 49,8 Mb
Release : 2022-12-12
Category : Medical
ISBN : 9781000644821

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Flow Cytometry in Neoplastic Hematology by Wojciech Gorczyca Pdf

This fourth edition presents an updated and expanded text and illustrations to reflect continued morphologic, immunophenotypic, and especially molecular advances in the field of neoplastic hematology, mostly due to the rapidly expanding application of next-generation sequencing. Those advances not only allow a more reliable diagnosis of the majority of tumors and identification of early changes such as monoclonal B-cell lymphocytosis or clonal hematopoiesis of indeterminate potential (CHIP), but also in many cases identify mutations or phenotypic changes in tumors that can be targeted by mutation-specific or antigen-specific drugs. This edition incorporates the updated WHO classification of hematopoietic tumors and new immunophenotypic and molecular markers to provide a thorough pathologic overview of hematologic neoplasms while focusing on flow cytometric features. Special emphasis has been put on hematological neoplasms with crucial clinical significance such as acute promyelocytic leukemia, other acute leukemias, and difficult areas in flow cytometry. Flow cytometric features in AML, MDS, CMML, CLL and measurable residual disease were significantly expanded. There are many new comparative tables, illustrations, and diagrams of algorithmic approaches.

Author : Sindhu Cherian,Brent Wood
Publisher : Unknown
Page : 165 pages
File Size : 46,6 Mb
Release : 2012
Category : Medical
ISBN : 0983706824

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Flow Cytometry in Evaluation of Hematopoietic Neoplasms by Sindhu Cherian,Brent Wood Pdf

Flow Cytometry in Evaluation of Hematopoietic Neoplasms: A Case-Based Approach is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. Using multi-color techniques pioneered by Brent Wood, the text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing). The publication contains an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem- cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion, enabling readers to develop and work through a differential diagnosis and recognize potential diagnostic pitfalls. Take-home points for each case emphasize critical concepts in flow cytometric diagnosis. Flow Cytometry in Evaluation of Hematopoietic Neoplasms provides pathologists, residents, laboratory technologists, and hematologists with both a study guide and an atlas for regular consultation in the clinical flow cytometry laboratory. The 2008 World Health Organization (WHO) classification system, French-American-British (FAB) classifications, and 2006 Bethesda Consensus recommendations are incorporated in the text.

Author : Amy S. Duffield,Joo Y. Song,Girish Venkataraman
Publisher : Lippincott Williams & Wilkins
Page : 1062 pages
File Size : 46,9 Mb
Release : 2020-10-09
Category : Medical
ISBN : 9781496375568

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Atlas of Lymph Node Pathology by Amy S. Duffield,Joo Y. Song,Girish Venkataraman Pdf

Closely mirroring the daily sign-out process, Atlas of Lymph Node Pathology: A Pattern Based Approach is a highly illustrated, efficient guide to accurate diagnosis. This practical reference uses a proven, pattern-based approach to clearly explain how to interpret challenging cases by highlighting red flags in the clinical chart and locating hidden clues in the slides. Useful as a daily “scope-side guide,” it features numerous clinical and educational features that help you find pertinent information, reach a correct diagnosis, and assemble a thorough and streamlined pathology report.

Author : Tsieh Sun
Publisher : Lww
Page : 276 pages
File Size : 45,6 Mb
Release : 2002
Category : Medical
ISBN : 0781729645

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Flow Cytometric Analysis of Hematologic Neoplasms by Tsieh Sun Pdf

This thoroughly updated Second Edition provides a comprehensive review of the basic principles, clinical applications, and interpretation of flow cytometry results. Focusing on hematologic neoplasms, it covers current uses and applications while considering cost containment trends in healthcare. More than 30 complete clinical cases with detailed clinical histories, flow cytometric findings, morphologic illustrations, and other pertinent information on molecular biological techniques, cytochemistry, and cytogenetics provide readers with complete clinical information to assist in diagnosis, as well as to hone skills. The text also features 6 new leukemias/lymphomas and 94 new color illustrations, including color photographs of peripheral blood smears, bone marrow smears, bone marrow core biopsies, and histologic sections of lymph nodes, spleen, liver and solid tumors.