Fluorescence Spectroscopy And Microscopy In Biology

Author : Radek Šachl,Mariana Amaro
Publisher : Springer Nature
Page : 529 pages
File Size : 40,8 Mb
Release : 2023-04-27
Category : Science
ISBN : 9783031303623

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Fluorescence Spectroscopy and Microscopy in Biology by Radek Šachl,Mariana Amaro Pdf

This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.

Author : Martin Hof,Rudolf Hutterer,V. Fidler
Publisher : Springer Science & Business Media
Page : 315 pages
File Size : 45,9 Mb
Release : 2006-01-27
Category : Science
ISBN : 9783540270041

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Fluorescence Spectroscopy in Biology by Martin Hof,Rudolf Hutterer,V. Fidler Pdf

Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Author : Yves Engelborghs,Antonie J. W. G. Visser
Publisher : Unknown
Page : 816 pages
File Size : 49,5 Mb
Release : 2014
Category : Fluorescence microscopy
ISBN : 1627036490

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Fluorescence Spectroscopy and Microscopy by Yves Engelborghs,Antonie J. W. G. Visser Pdf

Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

Author : Anonim
Publisher : Academic Press
Page : 502 pages
File Size : 48,8 Mb
Release : 1989-04-01
Category : Science
ISBN : 0080859283

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Fluorescence Microscopy of Living Cells in Culture, Part B by Anonim Pdf

Fluorescence Microscopy of Living Cells in Culture, Part B

Author : Ammasi Periasamy,Robert M. Clegg
Publisher : CRC Press
Page : 472 pages
File Size : 52,7 Mb
Release : 2009-07-06
Category : Science
ISBN : 1420078917

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FLIM Microscopy in Biology and Medicine by Ammasi Periasamy,Robert M. Clegg Pdf

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy’s (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular surroundings of biological molecules. Most of this improvement and discovery have occurred during the past decade, and, to date, information that would benefit a broad range of researchers remains scattered in the literature. Edited by two of the top pioneers in the field, FLIM Microscopy in Biology and Medicine presents the fundamentals of FLIM along with a number of advanced considerations so that a wider audience can appreciate recent and potential improvements that make it such a valuable tool. New Opportunities for Biomedical Researchers... New Challenges for Microscopy Researchers Discussion sections in all the chapters clearly show the challenges for implementing FLIM for various applications. Certain chapters discuss limits on the number of photons required for highly accurate lifetime determinations, as well as the accuracy with which multiple, closely associated lifetime components can reliably be determined. Such considerations are important for the user when he or she is selecting the most advantageous method of FLIM to use for a particular application. While this book provides an introduction for those new to FLIM, it gathers a wealth of material to enhance the work of experts involved in pioneering technological improvements, as well as those research opportunities in this unique and promising area of microscopy.

Author : Yves Engelborghs,Antonie J.W.G. Visser
Publisher : Humana
Page : 0 pages
File Size : 46,6 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493963155

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Fluorescence Spectroscopy and Microscopy by Yves Engelborghs,Antonie J.W.G. Visser Pdf

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Author : Brian Herman
Publisher : Springer
Page : 188 pages
File Size : 54,7 Mb
Release : 1998
Category : Medical
ISBN : UOM:49015002477850

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Flourescence Microscopy by Brian Herman Pdf

The fluorescence microscope is an important biological laboratory tool, used to detect dells or molecules that emit fluorescence. This book deals with the fundamental principles and techniques, covering topics such as immunofluorescence, in-situ hybridization, and fluorescence photomicrography. In addition, a complete troubleshooting guide with practical hints is provided. 60 illus.

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 504 pages
File Size : 42,7 Mb
Release : 2017-06-19
Category : Medical
ISBN : 9783527338375

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Author : Markus Sauer,Johan Hofkens,Jörg Enderlein
Publisher : John Wiley & Sons
Page : 425 pages
File Size : 44,5 Mb
Release : 2010-12-23
Category : Science
ISBN : 9783527633524

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Handbook of Fluorescence Spectroscopy and Imaging by Markus Sauer,Johan Hofkens,Jörg Enderlein Pdf

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Author : Denis Rousseau
Publisher : Elsevier
Page : 442 pages
File Size : 43,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323150217

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Optical Techniques in Biological Research by Denis Rousseau Pdf

Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.

Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
Publisher : CRC Press
Page : 574 pages
File Size : 54,8 Mb
Release : 2014-07-17
Category : Technology & Engineering
ISBN : 9781439861677

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Fluorescence Lifetime Spectroscopy and Imaging by Laura Marcu,Paul M. W. French,Daniel S. Elson Pdf

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.

Author : Natalia Grigoryeva
Publisher : BoD – Books on Demand
Page : 464 pages
File Size : 40,7 Mb
Release : 2020-09
Category : Cytofluorometry
ISBN : 9781839680397

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Fluorescence Methods for Investigation of Living Cells and Microorganisms by Natalia Grigoryeva Pdf

Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Author : Bernard Valeur,Jean-Claude Brochon
Publisher : Springer Science & Business Media
Page : 496 pages
File Size : 51,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642568534

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New Trends in Fluorescence Spectroscopy by Bernard Valeur,Jean-Claude Brochon Pdf

This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Author : Anda Cornea,P. Michael Conn
Publisher : Elsevier
Page : 260 pages
File Size : 55,9 Mb
Release : 2014-02-24
Category : Science
ISBN : 9780124167131

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Fluorescence Microscopy by Anda Cornea,P. Michael Conn Pdf

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Author : Amadeo J. Pesce
Publisher : Unknown
Page : 247 pages
File Size : 44,7 Mb
Release : 1995
Category : Electronic
ISBN : 0317295667

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Fluorescence Spectroscopy by Amadeo J. Pesce Pdf