Handbook Of Fluorescence Spectroscopy And Imaging

Author : Markus Sauer,Johan Hofkens,Jörg Enderlein
Publisher : John Wiley & Sons
Page : 425 pages
File Size : 54,6 Mb
Release : 2010-12-23
Category : Science
ISBN : 9783527633524

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Handbook of Fluorescence Spectroscopy and Imaging by Markus Sauer,Johan Hofkens,Jörg Enderlein Pdf

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Author : Mary-Ann Mycek,Brian W. Pogue
Publisher : CRC Press
Page : 702 pages
File Size : 40,6 Mb
Release : 2019-07-17
Category : Medical
ISBN : 0203912098

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Handbook of Biomedical Fluorescence by Mary-Ann Mycek,Brian W. Pogue Pdf

Melding basic and clinical science, this reference provides a comprehensive overview of the roles that biophysics, photochemistry, and computational modeling play in the biomedical applications of fluorescence spectroscopy and imaging. Penned by pioneering researchers, the Handbook of Biomedical Fluorescence discusses fundamental aspects of fluorescence generation in organic molecules within tissue, theoretical and experimental views of how light propagation in tissue can be used to interpret fluorescence signals, endogenous and exogenous fluorescence agents in medical or basic research studies, and radiation transport, diffusion theory, and the Monte Carlo method.

Author : Chris Gell,David Brockwell,Alastair Smith
Publisher : Oxford University Press on Demand
Page : 279 pages
File Size : 44,9 Mb
Release : 2006-08-17
Category : Science
ISBN : 9780198529422

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Handbook of Single Molecule Fluorescence Spectroscopy by Chris Gell,David Brockwell,Alastair Smith Pdf

This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
Publisher : CRC Press
Page : 554 pages
File Size : 42,5 Mb
Release : 2014-07-17
Category : Science
ISBN : 9781439861684

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Fluorescence Lifetime Spectroscopy and Imaging by Laura Marcu,Paul M. W. French,Daniel S. Elson Pdf

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Author : Chris Gell,David Brockwell,D. Alastair Smith
Publisher : Unknown
Page : 0 pages
File Size : 51,6 Mb
Release : 2023
Category : Fluorescence spectroscopy
ISBN : 1383024871

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Handbook of Single Molecule Fluorescence Spectroscopy by Chris Gell,David Brockwell,D. Alastair Smith Pdf

This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Author : Guy Cox
Publisher : CRC Press
Page : 430 pages
File Size : 45,9 Mb
Release : 2019-04-23
Category : Science
ISBN : 9781351129381

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Fundamentals of Fluorescence Imaging by Guy Cox Pdf

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Author : Ruud Kraayenhof,Antonie J. W. G. Visser
Publisher : Springer Science & Business Media
Page : 424 pages
File Size : 48,5 Mb
Release : 2002
Category : Medical
ISBN : UOM:39015056257796

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Fluorescence Spectroscopy, Imaging, and Probes by Ruud Kraayenhof,Antonie J. W. G. Visser Pdf

The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.

Author : G¿nter Gauglitz,Tuan Vo-Dinh
Publisher : John Wiley & Sons
Page : 1168 pages
File Size : 49,8 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527605026

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Handbook of Spectroscopy by G¿nter Gauglitz,Tuan Vo-Dinh Pdf

This handbook provides a straightforward introduction to spectroscopy, showing what it can do and how it does it, together with a clear, integrated and objective account of the wealth of information that can be derived from spectra. The sequence of chapters covers a wide range of the electromagnetic spectrum, and the physical processes involved, from nuclear phenomena to molecular rotation processes. - A day-by-day laboratory guide: its design based on practical knowledge of spectroscopists at universities, industries and research institutes - A well-structured information source containing methods and applications sections framed by sections on general topics - Guides users to a decision about which spectroscopic method and which instrumentation will be the most appropriate to solve their own practical problem - Rapid access to essential information - Correct analysis of a huge number of measured spectra data and smart use of such information sources as databases and spectra libraries

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 55,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461576587

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Author : T.S.L Radhika,T. K.V. Iyengar,T. Raja Rani
Publisher : CRC Press
Page : 584 pages
File Size : 42,7 Mb
Release : 2014-10-31
Category : Mathematics
ISBN : 9781466588134

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Approximate Analytical Methods for Solving Ordinary Differential Equations by T.S.L Radhika,T. K.V. Iyengar,T. Raja Rani Pdf

Approximate Analytical Methods for Solving Ordinary Differential Equations (ODEs) is the first book to present all of the available approximate methods for solving ODEs, eliminating the need to wade through multiple books and articles. It covers both well-established techniques and recently developed procedures, including the classical series solution method, diverse perturbation methods, pioneering asymptotic methods, and the latest homotopy methods. The book is suitable not only for mathematicians and engineers but also for biologists, physicists, and economists. It gives a complete description of the methods without going deep into rigorous mathematical aspects. Detailed examples illustrate the application of the methods to solve real-world problems. The authors introduce the classical power series method for solving differential equations before moving on to asymptotic methods. They next show how perturbation methods are used to understand physical phenomena whose mathematical formulation involves a perturbation parameter and explain how the multiple-scale technique solves problems whose solution cannot be completely described on a single timescale. They then describe the Wentzel, Kramers, and Brillown (WKB) method that helps solve both problems that oscillate rapidly and problems that have a sudden change in the behavior of the solution function at a point in the interval. The book concludes with recent nonperturbation methods that provide solutions to a much wider class of problems and recent analytical methods based on the concept of homotopy of topology.

Author : Burkhard Beckhoff,Birgit Kanngießer,Norbert Langhoff,Reiner Wedell,Helmut Wolff
Publisher : Springer Science & Business Media
Page : 897 pages
File Size : 54,9 Mb
Release : 2007-05-18
Category : Science
ISBN : 9783540367222

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Handbook of Practical X-Ray Fluorescence Analysis by Burkhard Beckhoff,Birgit Kanngießer,Norbert Langhoff,Reiner Wedell,Helmut Wolff Pdf

X-Ray fluorescence analysis is an established technique for non-destructive elemental materials analysis. This book gives a user-oriented practical guidance to the application of this method. The book gives a survey of the theoretical fundamentals, analytical instrumentation, software for data processing, various excitation regimes including gracing incidents and microfocus measurements, quantitative analysis, applications in routine and micro analysis, mineralogy, biology, medicine, criminal investigations, archeology, metallurgy, abrasion, microelectronics, environmental air and water analysis. This book is the bible of X-Ray fluorescence analysis. It gives the basic knowledge on this technique, information on analytical equipment and guides the reader to the various applications. It appeals to researchers, analytically active engineers and advanced students.

Author : Tuan Vo-Dinh
Publisher : CRC Press
Page : 1928 pages
File Size : 51,6 Mb
Release : 2003-03-26
Category : Medical
ISBN : 0849311160

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Biomedical Photonics Handbook by Tuan Vo-Dinh Pdf

A wide variety of biomedical photonic technologies have been developed recently for clinical monitoring of early disease states; molecular diagnostics and imaging of physiological parameters; molecular and genetic biomarkers; and detection of the presence of pathological organisms or biochemical species of clinical importance. However, available information on this rapidly growing field is fragmented among a variety of journals and specialized books. Now researchers and medical practitioners have an authoritative and comprehensive source for the latest research and applications in biomedical photonics. Over 150 leading scientists, engineers, and physicians discuss state-of-the-art instrumentation, methods, and protocols in the Biomedical Photonics Handbook. Editor-in-Chief Tuan Vo-Dinh and an advisory board of distinguished scientists and medical experts ensure that each of the 65 chapters represents the latest and most accurate information currently available.

Author : Zygmunt (Karol) Gryczynski,Ignacy Gryczynski
Publisher : CRC Press
Page : 772 pages
File Size : 41,8 Mb
Release : 2019-12-18
Category : Medical
ISBN : 9781439821701

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Practical Fluorescence Spectroscopy by Zygmunt (Karol) Gryczynski,Ignacy Gryczynski Pdf

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Author : Alexander P. Demchenko
Publisher : Springer Nature
Page : 772 pages
File Size : 53,7 Mb
Release : 2023-02-15
Category : Medical
ISBN : 9783031190896

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. This book provides systematic knowledge of basic principles in design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Author : Joseph R. Lakowicz
Publisher : Unknown
Page : 128 pages
File Size : 43,9 Mb
Release : 1991
Category : Fluorescence spectroscopy
ISBN : LCCN:91032671

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Topics in Fluorescence Spectroscopy: Techniques by Joseph R. Lakowicz Pdf