Fluorescent Materials For Cell Imaging

Author : Fu-Gen Wu
Publisher : Springer Nature
Page : 247 pages
File Size : 42,6 Mb
Release : 2020-10-26
Category : Medical
ISBN : 9789811550621

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Fluorescent Materials for Cell Imaging by Fu-Gen Wu Pdf

This book focuses on the latest fluorescent materials for cell imaging. Cell imaging is a widely used basic technique that helps scientists gain a better understanding of biological functions through studies of cellular structure and dynamics. In the past decades, the development of a variety of new fluorescent materials has significantly extended the applications of cellular imaging techniques. This book presents recently developed fluorescent materials, including semiconductor quantum dots, carbon dots, silicon nanoparticles, metal nanoclusters, upconversion nanoparticles, conjugated polymers/polymer dots, aggregation-induced emission (AIE) probes, and coordination compounds, used for various cellular imaging purposes. It will appeal to cell biologists and other researchers in academia, industry and clinical settings who are interested in the technical development and advanced applications of fluorescence imaging in cells, tissues and organisms to explore the mechanisms of biological functions and diseases.

Author : Vivek Mishra,Syed Sibtay Razi,Ajit Kumar
Publisher : CRC Press
Page : 347 pages
File Size : 54,5 Mb
Release : 2024-06-17
Category : Technology & Engineering
ISBN : 9781040036778

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Functional Fluorescent Materials by Vivek Mishra,Syed Sibtay Razi,Ajit Kumar Pdf

Functional Fluorescent Materials: Applications in Sensing, Bioimaging, and Optoelectronics explains functional molecular probes (organic/inorganic materials, polymers, nanomaterials), with a focus on those that represent spectroscopic properties with detection of different analytes and specific roles in molecular recognition and their applications. It broadly covers molecular recognition to applications of fluorescence reporters, starting from optoelectronic properties of materials, detection of heavy metals, through biological macromolecules, and further to a living cell, tissue imaging, and theranostics. Features: • Covers different aspects of fluorescence spectroscopy ranging from chemical, physical, and biological aspects along with optoelectronic properties, mechanisms, and applications. • Describes all types of chemical and functionalized fluorescent nanomaterials. • Provides additional information on different kinds of fluorescence reporters. • Explains the concept of fluorescence spectroscopy and its role in human health care. • Discusses changes in static and dynamic properties of fluorescent probes and molecular recognitions. This book is aimed at graduate students and researchers in materials, chemical engineering, and engineering physics.

Author : Vivek Mishra,Syed Sibtay Razi,Ajit Kumar
Publisher : Unknown
Page : 0 pages
File Size : 51,8 Mb
Release : 2024-06
Category : Science
ISBN : 1032402989

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Functional Fluorescent Materials by Vivek Mishra,Syed Sibtay Razi,Ajit Kumar Pdf

Author : Anonim
Publisher : Academic Press
Page : 502 pages
File Size : 40,6 Mb
Release : 1989-04-01
Category : Science
ISBN : 0080859283

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Fluorescence Microscopy of Living Cells in Culture, Part B by Anonim Pdf

Fluorescence Microscopy of Living Cells in Culture, Part B

Author : Alexander P. Demchenko
Publisher : Springer Nature
Page : 772 pages
File Size : 50,7 Mb
Release : 2023-02-15
Category : Medical
ISBN : 9783031190896

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. This book provides systematic knowledge of basic principles in design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Author : Natalia Grigoryeva
Publisher : BoD – Books on Demand
Page : 464 pages
File Size : 47,9 Mb
Release : 2020-09
Category : Cytofluorometry
ISBN : 9781839680397

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Fluorescence Methods for Investigation of Living Cells and Microorganisms by Natalia Grigoryeva Pdf

Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Author : J. Slavík
Publisher : Springer Science & Business Media
Page : 338 pages
File Size : 42,5 Mb
Release : 1996-10-31
Category : Science
ISBN : 0306453924

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Fluorescence Microscopy and Fluorescent Probes by J. Slavík Pdf

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Author : Alexander P. Demchenko
Publisher : Springer Nature
Page : 673 pages
File Size : 52,6 Mb
Release : 2020-12-01
Category : Medical
ISBN : 9783030601553

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Author : David Stephens
Publisher : Scion Publishing Ltd
Page : 337 pages
File Size : 48,9 Mb
Release : 2005-12-01
Category : Science
ISBN : 9781907904387

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Cell Imaging by David Stephens Pdf

This book covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but is equally relevant to the imaging of fixed specimens.

Author : Raffaello Papadakis
Publisher : BoD – Books on Demand
Page : 110 pages
File Size : 53,8 Mb
Release : 2023-11-22
Category : Science
ISBN : 9781803551838

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Fluorescence Imaging - Recent Advances and Applications by Raffaello Papadakis Pdf

Fluorescence imaging is widely used in scientific fields ranging from biology to biomedicine and even materials science. The development of novel fluorescent labels and microscopy techniques has rendered fluorescence imaging profoundly useful. Particularly in bioscience, fluorescence imaging empowers the study of the intracellular distribution, dynamics, gene expression, protein–protein interactions, and protein localization and enables the identification and tracking of lysosomes. Fluorescence imaging is applicable in cells and tissues and is constantly gaining attention in medicine too, in the fields of fluorescence-guided surgery and robotic-assisted fluorescence surgery. Acknowledging all these important new trends, this book provides an overview of the recent advances and applications in fluorescence imaging.

Author : Ammasi Periasamy
Publisher : Springer
Page : 473 pages
File Size : 48,7 Mb
Release : 2013-05-27
Category : Science
ISBN : 9781461475132

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Methods in Cellular Imaging by Ammasi Periasamy Pdf

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Author : Raquel Seruca,Jasjit S. Suri,J. Miquel Sanches
Publisher : CRC Press
Page : 376 pages
File Size : 42,7 Mb
Release : 2017-09-06
Category : Medical
ISBN : 9781351647335

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Fluorescence Imaging and Biological Quantification by Raquel Seruca,Jasjit S. Suri,J. Miquel Sanches Pdf

This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.

Author : Robert Hoffman
Publisher : Humana Press
Page : 269 pages
File Size : 44,9 Mb
Release : 2012-06-15
Category : Medical
ISBN : 1617797987

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In Vivo Cellular Imaging Using Fluorescent Proteins by Robert Hoffman Pdf

The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.

Author : Sung-Bae Kim
Publisher : Humana
Page : 447 pages
File Size : 43,6 Mb
Release : 2021-05-29
Category : Science
ISBN : 1071612573

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Live Cell Imaging by Sung-Bae Kim Pdf

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Author : Waseem A. Wani,Mohammad Shahid,Afzal Hussain,Mohamed Fahad AlAjmi
Publisher : Springer
Page : 63 pages
File Size : 40,8 Mb
Release : 2018-09-27
Category : Science
ISBN : 9789811326554

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Fluorescent Organic Nanoparticles by Waseem A. Wani,Mohammad Shahid,Afzal Hussain,Mohamed Fahad AlAjmi Pdf

This book provides a critical review of recent advances in the development of fluorescent organic nanoparticles as materials of choice for the design and fabrication of sensors, bioimaging agents and drug delivery systems. The properties and functions of nanoparticles differ significantly from those of their parent entities or their bulk phases. Two of their most important features are their increased surface-to volume ratio, and the formation of surface structures differing from those in their bulk phases. In addition, the book discusses the synthesis of fluorescent conjugated polymers, self-assembled fluorescent nanoparticles, polydopamine nanoparticles, and aggregation-induced-emission or aggregation-induced-emission enhancement nanomaterials. In closing, the book provides an outlook on future research and development in fluorescent organic nanoparticles as smart materials with an impressive range of potential applications.