Live Cell Imaging

Author : Sung-Bae Kim
Publisher : Humana
Page : 447 pages
File Size : 41,8 Mb
Release : 2022-06-12
Category : Science
ISBN : 1071612603

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Live Cell Imaging by Sung-Bae Kim Pdf

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Author : Robert D. Goldman,David L. Spector
Publisher : CSHL Press
Page : 658 pages
File Size : 40,7 Mb
Release : 2005
Category : Science
ISBN : 0879696834

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Live Cell Imaging by Robert D. Goldman,David L. Spector Pdf

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

Author : Dmitri Papkovsky
Publisher : Humana Press
Page : 367 pages
File Size : 48,6 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493961403

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Live Cell Imaging by Dmitri Papkovsky Pdf

Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.

Author : P. Michael Conn
Publisher : Academic Press
Page : 594 pages
File Size : 49,7 Mb
Release : 2012-02
Category : Medical
ISBN : 9780123884480

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Imaging and Spectroscopic Analysis of Living Cells by P. Michael Conn Pdf

This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

Author : Ruslan I. Dmitriev
Publisher : Springer
Page : 172 pages
File Size : 51,5 Mb
Release : 2017-10-26
Category : Science
ISBN : 9783319673585

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Multi-Parametric Live Cell Microscopy of 3D Tissue Models by Ruslan I. Dmitriev Pdf

This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.

Author : Michelle S. Itano,Paul Maddox,Abhishek Kumar
Publisher : Frontiers Media SA
Page : 135 pages
File Size : 43,9 Mb
Release : 2023-04-28
Category : Science
ISBN : 9782832522127

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Live cell imaging: Cell and developmental research bridging education, optical engineering, industry, software, shared facilities by Michelle S. Itano,Paul Maddox,Abhishek Kumar Pdf

Author : David Stephens
Publisher : Scion Publishing Ltd
Page : 337 pages
File Size : 42,5 Mb
Release : 2005-12-01
Category : Science
ISBN : 9781907904387

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Cell Imaging by David Stephens Pdf

This book covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but is equally relevant to the imaging of fixed specimens.

Author : P. Michael Conn
Publisher : Academic Press
Page : 559 pages
File Size : 52,8 Mb
Release : 2012
Category : Cell physiology
ISBN : 9780123918567

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Imaging and Spectroscopic Analysis of Living Cells by P. Michael Conn Pdf

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Publisher's note.

Author : Anonim
Publisher : Academic Press
Page : 588 pages
File Size : 41,5 Mb
Release : 2014-06-25
Category : Science
ISBN : 9780124202016

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Quantitative Imaging in Cell Biology by Anonim Pdf

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Author : Barry R. Masters
Publisher : SPIE Press
Page : 236 pages
File Size : 45,5 Mb
Release : 2006
Category : Confocal microscopy
ISBN : 0819461180

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Confocal Microscopy and Multiphoton Excitation Microscopy by Barry R. Masters Pdf

This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.

Author : P. Michael Conn
Publisher : Academic Press
Page : 574 pages
File Size : 42,6 Mb
Release : 2012-01-24
Category : Medical
ISBN : 9780123918574

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Imaging and Spectroscopic Analysis of Living Cells by P. Michael Conn Pdf

This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

Author : Douglas J. Taatjes,Brooke T. Mossman
Publisher : Springer Science & Business Media
Page : 505 pages
File Size : 50,7 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592599936

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Cell Imaging Techniques by Douglas J. Taatjes,Brooke T. Mossman Pdf

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Author : Margarida Barroso,Xavier Intes
Publisher : CRC Press
Page : 444 pages
File Size : 54,5 Mb
Release : 2020-12-03
Category : Science
ISBN : 9781351704496

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Imaging from Cells to Animals In Vivo by Margarida Barroso,Xavier Intes Pdf

This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.

Author : Anonim
Publisher : Academic Press
Page : 560 pages
File Size : 46,9 Mb
Release : 2012-01-25
Category : Science
ISBN : 9780123918697

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Imaging and Spectroscopic Analysis of Living Cells by Anonim Pdf

This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

Author : Joachim Wegener
Publisher : Springer Nature
Page : 286 pages
File Size : 41,7 Mb
Release : 2019-11-18
Category : Science
ISBN : 9783030324339

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Label-Free Monitoring of Cells in vitro by Joachim Wegener Pdf

This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.