Introduction To Fluorescence Sensing

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Introduction to Fluorescence Sensing

Alexander P. Demchenko
Introduction to Fluorescence Sensing Book PDF

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
Page : 606 pages
File Size : 55,9 Mb
Release : 2008-12-21
Category : Medical
ISBN : 9781402090035

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.

Introduction to Fluorescence Sensing

Alexander P. Demchenko
Introduction to Fluorescence Sensing Book PDF

Author : Alexander P. Demchenko
Publisher : Springer
Page : 794 pages
File Size : 51,9 Mb
Release : 2015-10-06
Category : Medical
ISBN : 9783319207803

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Introduction to Fluorescence Sensing

Alexander P. Demchenko
Introduction to Fluorescence Sensing Book PDF

Author : Alexander P. Demchenko
Publisher : Springer Nature
Page : 673 pages
File Size : 47,6 Mb
Release : 2020-12-01
Category : Medical
ISBN : 9783030601553

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

This book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Introduction to Fluorescence Sensing

Alexander P. Demchenko
Introduction to Fluorescence Sensing Book PDF

Author : Alexander P. Demchenko
Publisher : Springer Nature
Page : 772 pages
File Size : 41,6 Mb
Release : 2023-02-15
Category : Medical
ISBN : 9783031190896

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

Fluorescence is the most popular technique in chemical and biological sensing because of its ultimate sensitivity, high temporal and spatial resolution and versatility that enables imaging within the living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response up to detection of single molecules. Its application areas range from control of industrial processes to environment monitoring and clinical diagnostics. This book provides systematic knowledge of basic principles in design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Being a guide for students and young researchers, it also addresses professionals involved in active basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Principles of Fluorescence Spectroscopy

Joseph R. Lakowicz
Principles of Fluorescence Spectroscopy Book PDF

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 46,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461576587

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Advanced Concepts in Fluorescence Sensing

Chris D. Geddes,Joseph R. Lakowicz
Advanced Concepts in Fluorescence Sensing Book PDF

Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 346 pages
File Size : 48,8 Mb
Release : 2005-06-28
Category : Science
ISBN : 0387233342

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Advanced Concepts in Fluorescence Sensing by Chris D. Geddes,Joseph R. Lakowicz Pdf

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Introduction to Confocal Fluorescence Microscopy

Michiel Muller
Introduction to Confocal Fluorescence Microscopy Book PDF

Author : Michiel Muller
Publisher : SPIE Press
Page : 142 pages
File Size : 45,9 Mb
Release : 2006
Category : Science
ISBN : 0819460435

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Introduction to Confocal Fluorescence Microscopy by Michiel Muller Pdf

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Molecular Fluorescence

Bernard Valeur,Mário Nuno Berberan-Santos
Molecular Fluorescence Book PDF

Author : Bernard Valeur,Mário Nuno Berberan-Santos
Publisher : John Wiley & Sons
Page : 595 pages
File Size : 55,9 Mb
Release : 2013-03-25
Category : Science
ISBN : 9783527328468

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Molecular Fluorescence by Bernard Valeur,Mário Nuno Berberan-Santos Pdf

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Metal Nanoclusters in Catalysis and Materials Science: The Issue of Size Control

Benedetto Corain,Guenter Schmid,N Toshima
Metal Nanoclusters in Catalysis and Materials Science The Issue of Size Control Book PDF

Author : Benedetto Corain,Guenter Schmid,N Toshima
Publisher : Elsevier
Page : 471 pages
File Size : 54,9 Mb
Release : 2011-08-11
Category : Technology & Engineering
ISBN : 9780080555003

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Metal Nanoclusters in Catalysis and Materials Science: The Issue of Size Control by Benedetto Corain,Guenter Schmid,N Toshima Pdf

Metal Nanoclusters in Catalysis and Materials Science: The Issue of Size Control deals with the synthesis of metal nanoclusters along all known methodologies. Physical and chemical properties of metal nanoclusters relevant to their applications in chemical processing and materials science are covered thoroughly. Special attention is given to the role of metal nanoclusters size and shape in catalytic processes and catalytic applications relevant to industrial chemical processing.An excellent text for expanding the knowledge on the chemistry and physics of metal nanoclusters. Divided in two parts; Part I deals with general aspects of the matter and Part II has to be considered a useful handbook dealing with the production of metal nanoclusters, especially from their size-control point of view. * Divided into two parts for ease of reference: general and operational * Separation of synthetic aspects, physical properties and applications* Specific attention is given to the task of metal nanoclusters size-control

Fluorescence Sensors and Biosensors

Richard B. Thompson
Fluorescence Sensors and Biosensors Book PDF

Author : Richard B. Thompson
Publisher : CRC Press
Page : 416 pages
File Size : 41,5 Mb
Release : 2019-08-30
Category : Electronic
ISBN : 0367391511

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Fluorescence Sensors and Biosensors by Richard B. Thompson Pdf

Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts. The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations. The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.

Topics in Fluorescence Spectroscopy

Joseph R. Lakowicz
Topics in Fluorescence Spectroscopy Book PDF

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 502 pages
File Size : 52,9 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306470608

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Topics in Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Advanced Concepts in Fluorescence Sensing

Chris D. Geddes,Joseph R. Lakowicz
Advanced Concepts in Fluorescence Sensing Book PDF

Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer
Page : 0 pages
File Size : 53,7 Mb
Release : 2008-11-01
Category : Science
ISBN : 0387503013

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Advanced Concepts in Fluorescence Sensing by Chris D. Geddes,Joseph R. Lakowicz Pdf

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Principles of Fluorescence Spectroscopy

Joseph R. Lakowicz
Principles of Fluorescence Spectroscopy Book PDF

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 708 pages
File Size : 51,7 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475730616

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Aquatic Organic Matter Fluorescence

Paula G. Coble,Jamie Lead,Andy Baker,Darren M. Reynolds,Robert G. M. Spencer
Aquatic Organic Matter Fluorescence Book PDF

Author : Paula G. Coble,Jamie Lead,Andy Baker,Darren M. Reynolds,Robert G. M. Spencer
Publisher : Cambridge University Press
Page : 407 pages
File Size : 48,7 Mb
Release : 2014-07-14
Category : Science
ISBN : 9780521764612

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Aquatic Organic Matter Fluorescence by Paula G. Coble,Jamie Lead,Andy Baker,Darren M. Reynolds,Robert G. M. Spencer Pdf

A core text on principles, laboratory/field methodologies, and data interpretation for fluorescence applications in aquatic science, for advanced students and researchers.

Introduction to Fluorescence

David M. Jameson
Introduction to Fluorescence Book PDF

Author : David M. Jameson
Publisher : Taylor & Francis
Page : 313 pages
File Size : 48,7 Mb
Release : 2014-01-22
Category : Science
ISBN : 9781439806050

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Introduction to Fluorescence by David M. Jameson Pdf

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.