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Author : C. Stolinski,A. S. Breathnach
Publisher : Unknown
Page : 192 pages
File Size : 44,9 Mb
Release : 1975
Category : Medical
ISBN : UOM:39015000286024
Author : C. Stolinski
Publisher : Unknown
Page : 173 pages
File Size : 41,8 Mb
Release : 1975
Category : Freeze fracturing
ISBN : OCLC:969343617
Author : M. A. Hayat
Publisher : Unknown
Page : 312 pages
File Size : 45,9 Mb
Release : 1970
Category : Science
ISBN : UOM:39015049446621
Freeze-substitution and freeze-drying; Freezing technology; The freeze-etching technique; Freeze-etching methodology; Intepretation of freeze-etching images; Negative staining; Equipment requirements; Negative staining; Summary comments on negative staining procedures; Photography of negatively stained specimens; Particle dimensions and molecular weights; Shadow casting and replication; High resolution and shadowing; Autoradiography; Conditions for quantitation; Analysis of autoradrograms; Technical considerations.
Author : Patrick Echlin
Publisher : Springer Science & Business Media
Page : 576 pages
File Size : 41,6 Mb
Release : 1992-03-31
Category : Science
ISBN : 0306439840
This comprehensive volume explores the preparation, examination, and analysis of organic, hydrated, and biological specimens using cryomicroscopic techniques. More than 200 illustrations supplement the text.
Author : H.C. Aldrich,W.J. Todd
Publisher : Springer Science & Business Media
Page : 540 pages
File Size : 46,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468451191
The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.
Author : J.K. Koehler
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 41,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642654923
The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Author : Anthony William Robards,Uwe Bernd Sleytr
Publisher : Elsevier Publishing Company
Page : 584 pages
File Size : 48,9 Mb
Release : 1985
Category : Science
ISBN : IND:30000121265213
Author : Roland A. Fleck,Bruno M. Humbel
Publisher : John Wiley & Sons
Page : 741 pages
File Size : 49,5 Mb
Release : 2019-04-29
Category : Science
ISBN : 9781118654064
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
Author : John E. Rash,C. Sue Hudson
Publisher : Unknown
Page : 226 pages
File Size : 50,6 Mb
Release : 1979
Category : Science
ISBN : UOM:39015000293806
Author : Anonim
Publisher : Unknown
Page : 510 pages
File Size : 43,6 Mb
Release : 1980
Category : Stereology
ISBN : UCSD:31822005513486
Author : Leo H Arnold
Publisher : CRC Press
Page : 179 pages
File Size : 50,6 Mb
Release : 2018-05-04
Category : Science
ISBN : 9781351086233
A comprehensive review of the yeast cell envelope has not appeared previously and therefore this book is timely. The title of this volume was chosen to reflect the three major areas of contribution to our current understanding of the cell envelope, but we have not attempted to group chapters into subdivisions. The approach was to describe phenomena, to review the literature and to illuminate outstanding problems. It was also attempted to generate working hypotheses which may stimulate further studies. The some of these ideas be of germinal value is of more concern to us than that all of the hypotheses should stand the test of further experimentation.
Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 43,7 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181
Author : C Hawes
Publisher : Academic Press
Page : 477 pages
File Size : 47,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323148986
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Author : John J. Bozzola,Lonnie Dee Russell
Publisher : Jones & Bartlett Learning
Page : 702 pages
File Size : 53,5 Mb
Release : 1999
Category : Medical
ISBN : 0763701920
New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR
Author : Heide Schatten
Publisher : Cambridge University Press
Page : 275 pages
File Size : 46,5 Mb
Release : 2013
Category : Science
ISBN : 9780521195997
A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.