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Author : Patrick Echlin
Publisher : Unknown
Page : 564 pages
File Size : 49,7 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 1489923039
Author : Patrick Echlin
Publisher : Springer Science & Business Media
Page : 553 pages
File Size : 52,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781489923028
The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.
Author : Patrick Echlin,B. Ralph,E. R. Weibel
Publisher : Unknown
Page : 311 pages
File Size : 48,5 Mb
Release : 1978
Category : Cryobiology
ISBN : 0632001038
Author : Arvid B. Maunsbach,Björn A. Afzelius
Publisher : Elsevier
Page : 548 pages
File Size : 46,8 Mb
Release : 1998-11-03
Category : Science
ISBN : 0080528090
This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs
Author : Rodenburg
Publisher : CRC Press
Page : 716 pages
File Size : 48,6 Mb
Release : 1997-01-01
Category : Technology & Engineering
ISBN : 0750304413
Electron Microscopy and Analysis 1997 celebrates the centenary anniversary of the discovery of the electron by J.J. Thomson in Cambridge and the fiftieth anniversary of this distinguished Institute group. The book includes papers on the early history of electron microscopy (from P. Hawkes), the development of the scanning electron microscope at Cambridge (from K. Smith), electron energy loss spectroscopy (from L.M. Brown), imaging methods (from J. Spence), and the future of electron microscopy (from C. Humphreys). Covering a wide range of applications of advanced techniques, it discusses electron imaging, electron energy-loss and x-ray analysis, and scanning probe and electron beam microscopies. This volume is a handy reference for professionals using microscopes in all areas of physics, materials science, metallurgy, and surface science to gain an overview of developments in our understanding of materials microstructure and of advances in microscope interrogation techniques.
Author : A. W. Robards
Publisher : Unknown
Page : 0 pages
File Size : 43,6 Mb
Release : 1985
Category : Electronic
ISBN : 0720442508
Author : Anthony William Robards,Uwe Bernd Sleytr
Publisher : Elsevier Publishing Company
Page : 584 pages
File Size : 52,6 Mb
Release : 1985
Category : Science
ISBN : IND:30000121265213
Author : Anonim
Publisher : Unknown
Page : 12 pages
File Size : 50,8 Mb
Release : 1973
Category : Electronic
ISBN : OCLC:918451644
Author : 0 J.R. Harris,
Publisher : Taylor & Francis
Page : 204 pages
File Size : 42,8 Mb
Release : 2023-05-09
Category : Science
ISBN : 9781000948943
Since the start of biological studies using the transmission electron microscope scientists have sought to develop procedures for the preparation and investigation of the thinly spread specimens of biological particulates. Negative Staining and Cryoelect
Author : Geoffrey R. Newman,Jan A. Hobot
Publisher : Springer Science & Business Media
Page : 225 pages
File Size : 52,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642974816
For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.
Author : Joseph Goldstein,Dale E. Newbury,Patrick Echlin,David C. Joy,Charles Fiori,Eric Lifshin
Publisher : Springer Science & Business Media
Page : 679 pages
File Size : 42,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461332732
This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.
Author : Arthur L. E. Barron
Publisher : Unknown
Page : 560 pages
File Size : 43,6 Mb
Release : 1995
Category : Crystals
ISBN : CORNELL:31924076496524
Vol. 3 adds section "The Entomological monthly."
Author : Michael Wink
Publisher : John Wiley & Sons
Page : 544 pages
File Size : 43,6 Mb
Release : 2020-12-03
Category : Science
ISBN : 9783527812868
Completely updated in line with the rapid progress made in the field, this new edition of the highly-praised textbook addresses powerful new methods and concepts in biotechnology, such as genome editing, reprogrammed stem cells, and personalized medicine. An introduction to the fundamentals in molecular and cell biology is followed by a description of standard techniques, including purification and analysis of biomolecules, cloning techniques, gene expression systems, genome editing methods, labeling of proteins and in situ-techniques, standard and high resolution microscopy. The third part focuses on key areas in research and application, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies and systems biology. The final part looks at the biotechnology industry, explaining intellectual property issues, legal frameworks for pharmaceutical products and the interplay between start-up and larger companies. The contents are beautifully illustrated throughout, with hundreds of full color diagrams and photographs. Provides students and professionals in life sciences, pharmacy and biochemistry with everything they need to know about molecular biotechnology.
Author : Patrick Echlin
Publisher : Springer Science & Business Media
Page : 329 pages
File Size : 47,9 Mb
Release : 2011-04-14
Category : Technology & Engineering
ISBN : 9780387857312
Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Author : Ian M. Watt
Publisher : Cambridge University Press
Page : 506 pages
File Size : 55,8 Mb
Release : 1997-01-30
Category : Science
ISBN : 0521435919
The first edition of this book was widely praised as an excellent introduction to electron microscopy for materials scientists, physicists, earth and biological scientists. This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practising electron microscopists. The coverage has been brought completely up to date, whilst retaining descriptions of early classic techniques. Currently live topics such as computer control of microscopes, energy-filtered imaging, cryo- and environmental microscopy, digital imaging, and high resolution scanning and transmission microscopy are all described. The highly praised case studies of the first edition have been expanded to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology.
