Handbook Of Proteomic Methods

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Handbook of Proteomic Methods

P. Michael Conn
Handbook of Proteomic Methods Book PDF

Author : P. Michael Conn
Publisher : Springer Science & Business Media
Page : 503 pages
File Size : 51,8 Mb
Release : 2003-07-10
Category : Science
ISBN : 9781592594146

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Handbook of Proteomic Methods by P. Michael Conn Pdf

A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.

The Proteomics Protocols Handbook

John M. Walker
The Proteomics Protocols Handbook Book PDF

Author : John M. Walker
Publisher : Springer Science & Business Media
Page : 969 pages
File Size : 40,9 Mb
Release : 2007-10-09
Category : Science
ISBN : 9781592598908

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The Proteomics Protocols Handbook by John M. Walker Pdf

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Proteomics

C. David O'Connor,B. David Hames
Proteomics Book PDF

Author : C. David O'Connor,B. David Hames
Publisher : Scion Publishing Ltd
Page : 289 pages
File Size : 42,6 Mb
Release : 2007-11-01
Category : Science
ISBN : 9781907904431

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Proteomics by C. David O'Connor,B. David Hames Pdf

Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation. The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong.

Proteomics in Practice

Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker
Proteomics in Practice Book PDF

Author : Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker
Publisher : John Wiley & Sons
Page : 502 pages
File Size : 50,6 Mb
Release : 2008-09-08
Category : Science
ISBN : 9783527622306

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Proteomics in Practice by Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker Pdf

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

Serum/Plasma Proteomics

David W. Greening,Richard J. Simpson
Serum Plasma Proteomics Book PDF

Author : David W. Greening,Richard J. Simpson
Publisher : Springer Nature
Page : 591 pages
File Size : 52,8 Mb
Release : 2023-02-13
Category : Science
ISBN : 9781071629789

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Serum/Plasma Proteomics by David W. Greening,Richard J. Simpson Pdf

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.

Proteomics in Practice

Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker
Proteomics in Practice Book PDF

Author : Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker
Publisher : Wiley-Blackwell
Page : 502 pages
File Size : 47,5 Mb
Release : 2008-05-05
Category : Science
ISBN : 3527319417

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Proteomics in Practice by Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker Pdf

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

Proteins and Proteomics

Richard J. Simpson
Proteins and Proteomics Book PDF

Author : Richard J. Simpson
Publisher : Unknown
Page : 950 pages
File Size : 53,7 Mb
Release : 2003
Category : Medical
ISBN : UOM:39015056839254

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Proteins and Proteomics by Richard J. Simpson Pdf

Introduction to proteomics; one-dimensional polyacrylamide gel electrophoresis; preparing cellular and subcellular extracts; preparative two-dimensional gel electrophoresis with immobilized pH gradients; reversed-phase high -performance liquid chromatography; amino- and carboxy-terminal sequence analysis; peptide mapping and sequence analysis of gel-resolved proteins; the use of mass spectrometry in proteomics; proteomic methods for phosphorylation site mapping; characterization of protein complexes; making sense of proteomics - using bioinformatics to discover a protein's structure, functions, and interactions.

Quantitative Proteome Analysis

Kazuhiro Imai,Sam Li Fong Yau
Quantitative Proteome Analysis Book PDF

Author : Kazuhiro Imai,Sam Li Fong Yau
Publisher : CRC Press
Page : 314 pages
File Size : 53,9 Mb
Release : 2013-08-05
Category : Science
ISBN : 9789814316514

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Quantitative Proteome Analysis by Kazuhiro Imai,Sam Li Fong Yau Pdf

This book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The first part of the book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and, especially, reproducibility. The second part of the book focuses on providing concise descriptions of the effective applications of these methods to demonstrate how they have resulted in many important discoveries of the roles of the proteins expressed in living cells.

Proteomic Profiling

Anton Posch
Proteomic Profiling Book PDF

Author : Anton Posch
Publisher : Humana
Page : 0 pages
File Size : 54,6 Mb
Release : 2016-10-29
Category : Science
ISBN : 1493949543

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Proteomic Profiling by Anton Posch Pdf

This volume presents the latest developments of the main pillars of protein analysis, such as sample preparation, separation and characterization. The book begins by describing basic but important sample preparation protocols. It then goes on to describe more sophisticated procedures on enriching specific protein classes and concludes with detailed descriptions of integrated work-flows for comprehensive protein analysis and characterization. The authors of the individual chapters are renowned protein biochemists who have all set value to provide a detailed representation of their lab work. Throughout the chapters, these authors share important tips and tricks for a successful and reproducible employment of their protocols in other laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomic Profiling: Methods and Protocols is the perfect guide for students of Biochemistry, Biomedicine, Biology, and Genomics and will be an invaluable source for the experienced, practicing scientists.

Clinical Proteomics

Fernando J. Corrales,Alberto Paradela,Miguel Marcilla
Clinical Proteomics Book PDF

Author : Fernando J. Corrales,Alberto Paradela,Miguel Marcilla
Publisher : Humana
Page : 0 pages
File Size : 42,9 Mb
Release : 2022-12-16
Category : Science
ISBN : 1071619381

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Clinical Proteomics by Fernando J. Corrales,Alberto Paradela,Miguel Marcilla Pdf

This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand the physicians’ toolbox for precision medicine-based patient care. From sample processing to multi-omics approaches, the book provides straight-forward protocols on a wide array of vital areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Clinical Proteomics: Methods and Protocols serves as an ideal guide for researchers working to expand upon the knowledge base needed to push forward toward a more personalized version of medicine.

Shotgun Proteomics

Daniel Martins-de-Souza
Shotgun Proteomics Book PDF

Author : Daniel Martins-de-Souza
Publisher : Unknown
Page : 492 pages
File Size : 49,5 Mb
Release : 2014
Category : Biochemistry
ISBN : 1493906852

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Shotgun Proteomics by Daniel Martins-de-Souza Pdf

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.

Modern Proteomics – Sample Preparation, Analysis and Practical Applications

Hamid Mirzaei,Martin Carrasco
Modern Proteomics Sample Preparation Analysis and Practical Applications Book PDF

Author : Hamid Mirzaei,Martin Carrasco
Publisher : Springer
Page : 533 pages
File Size : 52,6 Mb
Release : 2016-12-14
Category : Science
ISBN : 9783319414485

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Modern Proteomics – Sample Preparation, Analysis and Practical Applications by Hamid Mirzaei,Martin Carrasco Pdf

This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of: 1. The types of samples can be analyzed by mass spectrometry for proteomics analysis. 2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis. 3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins. 4. How various mass spectrometers work and different ways they can be used for proteomics analysis 5. The various platforms that are available for proteomics data analysis 6. The various applications of proteomics technologies in biological and medical sciences This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.

Clinical Proteomics

Antonia Vlahou,Manousos Makridakis
Clinical Proteomics Book PDF

Author : Antonia Vlahou,Manousos Makridakis
Publisher : Humana Press
Page : 0 pages
File Size : 51,5 Mb
Release : 2014-11-11
Category : Science
ISBN : 1493918710

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Clinical Proteomics by Antonia Vlahou,Manousos Makridakis Pdf

This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.

Manual of Cardiovascular Proteomics

Giulio Agnetti,Merry L. Lindsey,D. Brian Foster
Manual of Cardiovascular Proteomics Book PDF

Author : Giulio Agnetti,Merry L. Lindsey,D. Brian Foster
Publisher : Springer
Page : 430 pages
File Size : 49,8 Mb
Release : 2016-09-15
Category : Medical
ISBN : 9783319318288

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Manual of Cardiovascular Proteomics by Giulio Agnetti,Merry L. Lindsey,D. Brian Foster Pdf

This book fulfils the need to keep up with the high number of innovations in proteomics, and at the same time to warn the readers about the danger of manufacturers and scientists claims around new technologies. Mass spectrometry stands as the core technology in proteomics. The emerging field of targeted proteomics and its potential applications in the cardiovascular arena are also reviewed and discussed. A concluding section highilghts the promise of proteomics in the light of these recent developments.​ As this technique and its applications have undergone remarkable advances in the past years, recent updates on proteomic applications are covered. Another key concept revealed by proteomic technologies is that the extent of protein post-translational modifications (PTMs) as well as their impact on the phenotype has been underestimated by pre-proteomics science. As such, part of the manual focuses on the emerging role of PTMs in basic cardiovascular sciences and in the clinics. In fact, there is an emerging consensus that the detailed annotation of protein PTMs could lead to a more in-depth representation of biological systems, translating into more specific targets for therapy as well as biomarkers. Moreover, a recent trend is so-called “targeted proteomics”. The approach was awarded the title of “Method of the Year” by Nature in 2013 (see the editorial by Vivien Marx in 1st issue of Nature in Jan 2013). According to a few proteomic scientists the emphasis should not be placed on generating long lists of proteins but lists of proteins with a true biological meaning.

Proteomics in Practice

Reiner Westermeier,Tom Naven
Proteomics in Practice Book PDF

Author : Reiner Westermeier,Tom Naven
Publisher : Wiley-Blackwell
Page : 352 pages
File Size : 48,6 Mb
Release : 2002-08-23
Category : Medical
ISBN : UOM:39015056259214

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Proteomics in Practice by Reiner Westermeier,Tom Naven Pdf

Proteomics is a key area in the post-genomic era providing new insights into protein functions, interactions, and pathways. It is equally important for basic as well as applied research. This handbook takes a systematic approach to proteome analysis.