Handbook Of Single Cell Technologies

Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : Springer
Page : 1096 pages
File Size : 42,6 Mb
Release : 2021-10-29
Category : Technology & Engineering
ISBN : 9811089523

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Handbook of Single-Cell Technologies by Tuhin Subhra Santra,Fan-Gang Tseng Pdf

This book provides a brief overview of single-cell analysis using recent advanced technologies. The different sections cover different aspect of single cell analysis and applications with their advantages, limitations, and future challenges. The book has covered how different physical energies such as optical, electrical, and mechanical energy have been applied for single cell therapy and analysis. The recent advanced micro/nanofluidic devices have been employed for single-cell counting, manipulation, cultivation, separation, isolation, lysis, printing and patterning and host-viral interaction at single-cell level. Various chemical approaches for single-cell analysis have been discussed, such as liposome mediated materials transfer at single-cell and their analysis, discovery of antibody via single-cell, high-throughput screening of antigen-specific antibody-secreting cells, and biomolecular secretion analysis of individual cells. Moreover, different single-cell omics such as genomics, proteomics and transcriptomics have been discussed using microfluidic technologies as well as conventional approaches. The role of single cell analysis in system biology and biocatalysis have been discussed in detail. The book describes single-cell phenotyping of heterogeneous tissue, stimulation, and instant reaction quenching technology for biochemical kinetic analysis, large scale single-cell assay for the identification of biocatalysts and analytical techniques for single-cell studies in microbiology. The role of single-cell analysis in cancer, such as single-cell adhesion and cancer progression, single-cell technologies for cancer therapy, analytical technology for single cancer cell analysis, and biophysical markers for cancer cell analysis have been discussed. The flow cytometry based high throughput single-cell analysis have been well emphasized. Finally this book has covered single-cell electrophysiology, single-cell sensing and size measurement using mechanical and microwave resonators, molecular force spectroscopy for cell adhesion measurement, micro-tweezers and force microscopy techniques for single-cell mechanobiological analysis, mass spectrometry and acoustic tweezers for single-cell manipulation and analysis. This book is intended for academic and industrial researchers, undergraduate and graduate students in the fields of biomedical engineering, bio-micro/nanoengineering, and bio-micro/nano fabrication for single-cell analysis. It can be used for courses on bio-MEMS/bio-NEMS, biomicrofluidics, bio-micro/nanofabrications, micro/nanofluidics, biophysics, single cell analysis, bionanotechnology, drug delivery systems and biomedical microdevices. Collective contributions from respected experts, have brought diverse aspects of single-cell technologies in a single hand book. This will benefit researchers and practitioners in the biotechnology industry for different diseases analysis, therapeutics, diagnostics, drug discovery, drug screening etc. In addition to hard copies, the book will be available online and will often be updated by the authors.

Author : Guo-Cheng Yuan
Publisher : Unknown
Page : 271 pages
File Size : 53,8 Mb
Release : 2019
Category : Cytology
ISBN : 1493990578

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Computational Methods for Single-cell Data Analysis by Guo-Cheng Yuan Pdf

Author : Gregor Hoogers
Publisher : CRC Press
Page : 360 pages
File Size : 51,8 Mb
Release : 2002-09-27
Category : Technology & Engineering
ISBN : 9781420041552

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Fuel Cell Technology Handbook by Gregor Hoogers Pdf

Fuel cell systems have now reached a degree of technological maturity and appear destined to form the cornerstone of future energy technologies. But the rapid advances in fuel cell system development have left current information available only in scattered journals and Internet sites. The even faster race toward fuel cell commercialization further

Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : MDPI
Page : 254 pages
File Size : 45,7 Mb
Release : 2021-06-02
Category : Science
ISBN : 9783036506289

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Single Cell Analysis by Tuhin Subhra Santra,Fan-Gang Tseng Pdf

Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.

Author : Khawaja H. Haider
Publisher : Springer Nature
Page : 1422 pages
File Size : 49,5 Mb
Release : 2024-06-30
Category : Electronic
ISBN : 9789819971190

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Handbook of Stem Cell Applications by Khawaja H. Haider Pdf

Author : Henry Horng-Shing Lu,Bernhard Schölkopf,Martin T. Wells,Hongyu Zhao
Publisher : Springer Nature
Page : 406 pages
File Size : 50,8 Mb
Release : 2022-12-08
Category : Science
ISBN : 9783662659021

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Handbook of Statistical Bioinformatics by Henry Horng-Shing Lu,Bernhard Schölkopf,Martin T. Wells,Hongyu Zhao Pdf

Now in its second edition, this handbook collects authoritative contributions on modern methods and tools in statistical bioinformatics with a focus on the interface between computational statistics and cutting-edge developments in computational biology. The three parts of the book cover statistical methods for single-cell analysis, network analysis, and systems biology, with contributions by leading experts addressing key topics in probabilistic and statistical modeling and the analysis of massive data sets generated by modern biotechnology. This handbook will serve as a useful reference source for students, researchers and practitioners in statistics, computer science and biological and biomedical research, who are interested in the latest developments in computational statistics as applied to computational biology.

Author : Trygve O Tollefsbol
Publisher : Academic Press
Page : 920 pages
File Size : 41,7 Mb
Release : 2022-09-08
Category : Science
ISBN : 9780323919500

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Handbook of Epigenetics by Trygve O Tollefsbol Pdf

Handbook of Epigenetics: The New Molecular and Medical Genetics, Third Edition provides a comprehensive analysis of epigenetics, from basic biology to clinical application. This new edition has been fully revised to cover the latest and evolving topics in epigenetics, with chapters updated and new chapters added on topics such as single-cell epigenetics, DNA methylation clocks in age-related diseases, transposable elements and epigenetics, X chromosome inactivation, and the epigenetics of drug addiction, among other topics. Throughout this edition, greater emphasis falls on epigenomic analyses and incorporating multi-omics approaches rather than gene-specific analyses. In addition, this edition has also been enhanced with step-by-step instructions in research methods, as well as easy-to-digest disease case studies and clinical trials that provide context and applied examples of recent advances in disease understanding and epigenetic therapeutics. These features empower researchers to reproduce the approaches and studies discussed and aid clinical translation. Live links across chapters tie in relevant external datasets and resources. Provides a timely and comprehensive collection of fully up-to-date coverage of epigenetics Covers basic epigenetic biology, research methods and technology, disease relationships and clinical medicine Written at a verbal and technical level that can be understood by scientists and students alike, with chapter summaries and conclusions included throughout Discusses exciting new topics in epigenetics, such as DNA methylation clocks in age-related diseases, transposable elements and epigenetics, X chromosome inactivation, and the epigenetics of drug addiction Includes step-by-step instructions in research protocols to aid reproducibility, as well as easy-to-digest disease case studies and clinical trials, providing context and applied examples of recent clinical translation

Author : Tuhin S. Santra
Publisher : CRC Press
Page : 548 pages
File Size : 53,5 Mb
Release : 2020-11-01
Category : Medical
ISBN : 9781000769135

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Microfluidics and Bio-MEMS by Tuhin S. Santra Pdf

The past two decades have seen rapid development of micro-/nanotechnologies with the integration of chemical engineering, biomedical engineering, chemistry, and life sciences to form bio-MEMS or lab-on-chip devices that help us perform cellular analysis in a complex micro-/nanoflluidic environment with minimum sample consumption and have potential biomedical applications. To date, few books have been published in this field, and researchers are unable to find specialized content. This book compiles cutting-edge research on cell manipulation, separation, and analysis using microfluidics and bio-MEMS devices. It illustrates the use of micro-robots for biomedical applications, vascularized microfluidic organs-on-a-chip and their applications, as well as DNA gene microarray biochips and their applications. In addition, it elaborates on neuronal cell activity in microfluidic compartments, microvasculature and microarray gene patterning, different physical methods for drug delivery and analysis, micro-/nanoparticle preparation and separation in a micro-/nanofluidic environment, and the potential biomedical applications of micro-/nanoparticles. This book can be used by academic researchers, especially those involved in biomicrofluidics and bio-MEMS, and undergraduate- and graduate-level students of bio-MEMS/bio-nanoelectromechanical systems (bio-NEMS), biomicrofluidics, biomicrofabricatios, micro-/nanofluidics, biophysics, single-cell analysis, bionanotechnology, drug delivery systems, and biomedical micro-/nanodevices. Readers can gain knowledge of different aspects of microfluidics and bio-MEMS devices; their design, fabrication, and integration; and biomedical applications. The book will also help biotechnology-based industries, where research and development is ongoing in cell-based analysis, diagnosis, and drug screening.

Author : S.A. Sherif,D. Yogi Goswami,E.K. (Lee) Stefanakos,Aldo Steinfeld
Publisher : CRC Press
Page : 1058 pages
File Size : 52,5 Mb
Release : 2014-07-29
Category : Science
ISBN : 9781420054507

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Handbook of Hydrogen Energy by S.A. Sherif,D. Yogi Goswami,E.K. (Lee) Stefanakos,Aldo Steinfeld Pdf

Can hydrogen and electricity supply all of the world's energy needs? Handbook of Hydrogen Energy thoroughly explores the notion of a hydrogen economy and addresses this question. The handbook considers hydrogen and electricity as a permanent energy system and provides factual information based on science. The text focuses on a large cross section o

Author : Guo-Cheng Yuan
Publisher : Humana Press
Page : 271 pages
File Size : 49,9 Mb
Release : 2019-02-14
Category : Science
ISBN : 149399056X

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Computational Methods for Single-Cell Data Analysis by Guo-Cheng Yuan Pdf

This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis.

Author : Carlos Simón,Carmen Rubio
Publisher : CRC Press
Page : 409 pages
File Size : 42,5 Mb
Release : 2022-06-03
Category : Medical
ISBN : 9781000567366

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Handbook of Genetic Diagnostic Technologies in Reproductive Medicine by Carlos Simón,Carmen Rubio Pdf

Different genetic diagnostic and treatment options are used worldwide to improve routine IVF procedures for the benefit of patients. This handbook updates the new genetic diagnostic technologies that have been translated to the clinic, aiming to improve outcomes in the clinic and result in a healthy baby in the home. Chapters cover the use of genetic technologies in a personalized manner to unravel the possible genetic risks for the couple wishing to conceive, in terms of sperm, the embryo, the endometrium, miscarriage, and finally the fetus. This expanded new edition covers the range of the latest genetic diagnostic technologies being translated into practice internationally to improve routine IVF procedures for the benefit of patients. Bringing together international experts to discuss their work, this text gives a context for the developments in this very fast-moving area of research and offers a comprehensive and rounded appraisal of hot topics.

Author : Gary Durack,J. Paul Robinson
Publisher : John Wiley & Sons
Page : 375 pages
File Size : 52,7 Mb
Release : 2004-03-24
Category : Science
ISBN : 9780471461005

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Emerging Tools for Single-Cell Analysis by Gary Durack,J. Paul Robinson Pdf

The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,

Author : Debmalya Barh,Vasco Azevedo
Publisher : Academic Press
Page : 384 pages
File Size : 50,5 Mb
Release : 2019-07-31
Category : Electronic
ISBN : 9780128175323

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Single-Cell Omics by Debmalya Barh,Vasco Azevedo Pdf

Single-cell Omics, Volume 2: Advances in Applications provides the latest single-cell omics applications in the field of biomedicine. The advent of omics technologies have enabled us to identify the differences between cell types and subpopulations at the level of the genome, proteome, transcriptome, epigenome, and in several other fields of omics. The book is divided into two sections: the first is dedicated to biomedical applications, such as cell diagnostics, non-invasive prenatal testing (NIPT), circulating tumor cells, breast cancer, gliomas, nervous systems and autoimmune disorders, and more. The second focuses on cell omics in plants, discussing micro algal and single cell omics, and more. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and several members of biomedical field interested in understanding more about single-cell omics and its potential for research and diagnosis. Covers the diverse single cell omics applications in the biomedical field Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis Written by experts across the world, it brings different points-of-view and study cases to fully give a comprehensive overview of the topic

Author : Dongyou Liu
Publisher : CRC Press
Page : 763 pages
File Size : 41,8 Mb
Release : 2024-08-12
Category : Science
ISBN : 9781040005644

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Handbook of Molecular Biotechnology by Dongyou Liu Pdf

With a history that likely dates back to the dawn of human civilization more than 10,000 years ago, and a record that includes the domestication and selective breeding of plants and animals, the harnessing of fermentation process for bread, cheese, and brewage production, and the development of vaccines against infectious diseases, biotechnology has acquired a molecular focus during the 20th century, particularly following the resolution of DNA double helix in 1953, and the publication of DNA cloning protocol in 1973, and transformed our concepts and practices in disease diagnosis, treatment and prevention, pharmaceutical and industrial manufacturing, animal and plant industry, and food processing. While molecular biotechnology offers unlimited opportunities for improving human health and well-being, animal welfare, agricultural innovation and environmental conservation, a dearth of high quality books that have the clarity of laboratory manuals without distractive procedural details and the thoroughness of well-conversed textbooks appears to dampen the enthusiasm of aspiring students. In attempt to fill this glaring gap, Handbook of Molecular Biotechnology includes four sections, with the first three presenting in-depth coverage on DNA, RNA and protein technologies, and the fourth highlighting their utility in biotechnology. Recognizing the importance of logical reasoning and experimental verification over direct observation and simple description in biotechnological research and development, the Introduction provides pertinent discussions on key strategies (i.e., be first, be better, and be different), effective thinking (lateral, parallel, causal, reverse, and random), and experimental execution, which have proven invaluable in helping advance research projects, evaluate and prepare research reports, and enhance other scientific endeavors. Key features Presents state-of-the-art reviews on DNA, RNA and protein technologies and their biotechnological applications Discusses key strategies, effective thinking, and experimental execution for scientific research and development Fills the gap left by detailed-ridden laboratory manuals and insight-lacking standard textbooks Includes expert contributions from international scientists at the forefront of molecular biotechnology research and development Written by international scientists at the forefront of molecular biotechnology research and development, chapters in this volume cover the histories, principles, and applications of individual techniques/technologies, and constitute stand-alone, yet interlinked lectures that strive to educate as well as to entertain. Besides providing an informative textbook for tertiary students in molecular biotechnology and related fields, this volume serves as an indispensable roadmap for novice scientists in their efforts to acquire innovative skills and establish solid track records in molecular biotechnology, and offers a contemporary reference for scholars, educators, and policymakers wishing to keep in touch with recent developments in molecular biotechnology.

Author : H.A. Kiehne
Publisher : CRC Press
Page : 582 pages
File Size : 51,9 Mb
Release : 2003-08-29
Category : Technology & Engineering
ISBN : 9780824756420

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Battery Technology Handbook by H.A. Kiehne Pdf

This practical reference remains the most comprehensive guide to the fundamental theories, techniques, and strategies used for battery operation and design. It includes new and revised chapters focusing on the safety, performance, quality, and enhancement of various batteries and battery systems. From automotive, electrochemical, and high-energy applications to system implementation, selection, and standardization, the Second Edition presents expert discussions on electrochemical energy storage, the advantages of battery-powered traction, the disposal and recycling of used batteries, hazard prevention, and the chemistry and physics of lithium primary batteries.