Single Cell Analysis

Author : Guo-Cheng Yuan
Publisher : Humana Press
Page : 271 pages
File Size : 41,5 Mb
Release : 2019-02-14
Category : Science
ISBN : 149399056X

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Computational Methods for Single-Cell Data Analysis by Guo-Cheng Yuan Pdf

This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis.

Author : Jin-Ming Lin
Publisher : Springer Nature
Page : 261 pages
File Size : 43,7 Mb
Release : 2019-08-28
Category : Science
ISBN : 9789813297296

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Microfluidics for Single-Cell Analysis by Jin-Ming Lin Pdf

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Author : Harris G. Fienberg,Garry P. Nolan
Publisher : Springer
Page : 224 pages
File Size : 49,9 Mb
Release : 2014-04-22
Category : Medical
ISBN : 9783642548277

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High-Dimensional Single Cell Analysis by Harris G. Fienberg,Garry P. Nolan Pdf

This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.

Author : J. Paul Robinson,Andrea Cossarizza
Publisher : Springer
Page : 266 pages
File Size : 45,9 Mb
Release : 2017-04-18
Category : Technology & Engineering
ISBN : 9789811044991

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Single Cell Analysis by J. Paul Robinson,Andrea Cossarizza Pdf

This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings together an eclectic group of current applications, all of which have a significant impact on our current state of knowledge. The authors of these chapters are all pioneering researchers in the field of single cell analysis. The book will not only appeal to those readers more focused on clinical applications, but also those interested in highly technical aspects of the technologies. All of the technologies identified utilize unique applications of photon detection systems.

Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : Springer
Page : 414 pages
File Size : 44,5 Mb
Release : 2016-01-21
Category : Technology & Engineering
ISBN : 9783662491188

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Essentials of Single-Cell Analysis by Fan-Gang Tseng,Tuhin Subhra Santra Pdf

This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.

Author : Tuhin Subhra Santra,Fan-Gang Tseng
Publisher : MDPI
Page : 254 pages
File Size : 46,5 Mb
Release : 2021-06-02
Category : Science
ISBN : 9783036506289

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Single Cell Analysis by Tuhin Subhra Santra,Fan-Gang Tseng Pdf

Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.

Author : Yutaka Suzuki
Publisher : Springer
Page : 150 pages
File Size : 55,8 Mb
Release : 2019-04-09
Category : Medical
ISBN : 9789811360374

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Single Molecule and Single Cell Sequencing by Yutaka Suzuki Pdf

This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform. This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications. This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.

Author : Sara Lindström,Helene Andersson-Svahn
Publisher : Humana Press
Page : 238 pages
File Size : 49,7 Mb
Release : 2012-02-10
Category : Science
ISBN : 1617795666

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Single-Cell Analysis by Sara Lindström,Helene Andersson-Svahn Pdf

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail. The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.

Author : Dario Anselmetti
Publisher : John Wiley & Sons
Page : 284 pages
File Size : 41,8 Mb
Release : 2009-03-09
Category : Science
ISBN : 9783527626656

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Single Cell Analysis by Dario Anselmetti Pdf

The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.

Author : Jian Chen,Yao Lu
Publisher : Academic Press
Page : 184 pages
File Size : 50,5 Mb
Release : 2021-10-26
Category : Science
ISBN : 9780323885591

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Biosensors for Single-Cell Analysis by Jian Chen,Yao Lu Pdf

Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology

Author : Valentina Proserpio
Publisher : Unknown
Page : 452 pages
File Size : 41,5 Mb
Release : 2019
Category : Cytology
ISBN : 1493992422

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Single Cell Methods by Valentina Proserpio Pdf

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.

Author : Fan-Gang Tseng,Tuhin Subhra Santra
Publisher : MDPI
Page : 237 pages
File Size : 44,6 Mb
Release : 2018-10-01
Category : Electronic book
ISBN : 9783038421931

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Single Cell Analysis in Biotechnology and Systems Biology by Fan-Gang Tseng,Tuhin Subhra Santra Pdf

This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS

Author : Anonim
Publisher : Academic Press
Page : 326 pages
File Size : 54,7 Mb
Release : 2019-11-12
Category : Science
ISBN : 9780128170908

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Enzyme Activity in Single Cells by Anonim Pdf

Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Enzyme Activity in Single Cells

Author : Anup K. Singh,Aarthi Chandrasekaran
Publisher : Humana
Page : 0 pages
File Size : 46,6 Mb
Release : 2015-11-07
Category : Science
ISBN : 1493929860

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Single Cell Protein Analysis by Anup K. Singh,Aarthi Chandrasekaran Pdf

This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.

Author : Jialiang Yang,Liao Bo,Tuo Zhang,Yifei Xu
Publisher : Frontiers Media SA
Page : 136 pages
File Size : 43,5 Mb
Release : 2020-02-27
Category : Electronic
ISBN : 9782889635283

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Bioinformatics Analysis of Single Cell Sequencing Data and Applications in Precision Medicine by Jialiang Yang,Liao Bo,Tuo Zhang,Yifei Xu Pdf