Heterologous Expression Of Membrane Proteins

Heterologous Expression Of Membrane Proteins Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Heterologous Expression Of Membrane Proteins book. This book definitely worth reading, it is an incredibly well-written.

Heterologous Expression of Membrane Proteins

Isabelle Mus-Veteau
Heterologous Expression of Membrane Proteins Book PDF

Author : Isabelle Mus-Veteau
Publisher : Springer Nature
Page : 472 pages
File Size : 52,8 Mb
Release : 2022-06-30
Category : Science
ISBN : 9781071623688

Get Book

Heterologous Expression of Membrane Proteins by Isabelle Mus-Veteau Pdf

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Membrane Protein Expression Systems

Gwyn W. Gould
Membrane Protein Expression Systems Book PDF

Author : Gwyn W. Gould
Publisher : Ashgate Publishing
Page : 330 pages
File Size : 51,8 Mb
Release : 1994
Category : Science
ISBN : UOM:39015049078622

Get Book

Membrane Protein Expression Systems by Gwyn W. Gould Pdf

The use of heterologous expression as a means to study membrane proteins is becoming widespread. This book is a simple to read laboratory manual describing the principles behind the commonly used expression systems, eg baculovirus, oocytes, cell culture systems and yeast.

Heterologous Gene Expression in E.coli

Thomas C. Evans, Jr.,Ming-Qun Xu
Heterologous Gene Expression in E coli Book PDF

Author : Thomas C. Evans, Jr.,Ming-Qun Xu
Publisher : Humana Press
Page : 310 pages
File Size : 50,9 Mb
Release : 2011-08-24
Category : Medical
ISBN : 1617379689

Get Book

Heterologous Gene Expression in E.coli by Thomas C. Evans, Jr.,Ming-Qun Xu Pdf

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular BiologyTM format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Membrane Protein Protocols

Barry S. Selinsky
Membrane Protein Protocols Book PDF

Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 49,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592594009

Get Book

Membrane Protein Protocols by Barry S. Selinsky Pdf

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Production of Membrane Proteins

Anne Skaja Robinson
Production of Membrane Proteins Book PDF

Author : Anne Skaja Robinson
Publisher : John Wiley & Sons
Page : 631 pages
File Size : 45,7 Mb
Release : 2011-06-15
Category : Science
ISBN : 9783527634538

Get Book

Production of Membrane Proteins by Anne Skaja Robinson Pdf

Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages. Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations. Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale. Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

Membrane Proteins Production for Structural Analysis

Isabelle Mus-Veteau
Membrane Proteins Production for Structural Analysis Book PDF

Author : Isabelle Mus-Veteau
Publisher : Springer
Page : 441 pages
File Size : 43,7 Mb
Release : 2014-06-20
Category : Science
ISBN : 9781493906628

Get Book

Membrane Proteins Production for Structural Analysis by Isabelle Mus-Veteau Pdf

This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.

Membrane Proteins – Production and Functional Characterization

Anonim
Membrane Proteins Production and Functional Characterization Book PDF

Author : Anonim
Publisher : Academic Press
Page : 670 pages
File Size : 51,7 Mb
Release : 2015-04-06
Category : Science
ISBN : 9780128016251

Get Book

Membrane Proteins – Production and Functional Characterization by Anonim Pdf

Membrane Proteins – Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In Enzymology Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Heterologous Gene Expression in E.coli

Nicola A. Burgess-Brown
Heterologous Gene Expression in E coli Book PDF

Author : Nicola A. Burgess-Brown
Publisher : Humana Press
Page : 429 pages
File Size : 52,7 Mb
Release : 2018-07-20
Category : Science
ISBN : 1493983288

Get Book

Heterologous Gene Expression in E.coli by Nicola A. Burgess-Brown Pdf

This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.

Structural Genomics on Membrane Proteins

Kenneth H. Lundstrom
Structural Genomics on Membrane Proteins Book PDF

Author : Kenneth H. Lundstrom
Publisher : CRC Press
Page : 420 pages
File Size : 47,8 Mb
Release : 2006-02-23
Category : Medical
ISBN : 1420016083

Get Book

Structural Genomics on Membrane Proteins by Kenneth H. Lundstrom Pdf

While the genomic revolution has quickly led to the deposit of more than 30,000 structures in the protein data bank (PDB), less than one percent of those contributions represent membrane proteins despite the fact that membrane proteins constitute some 20 percent of all proteins. This discrepancy becomes significantly troublesome when it is coupled

Expression, Purification, and Structural Biology of Membrane Proteins

Camilo Perez,Timm Maier
Expression Purification and Structural Biology of Membrane Proteins Book PDF

Author : Camilo Perez,Timm Maier
Publisher : Unknown
Page : 423 pages
File Size : 53,8 Mb
Release : 2020
Category : Cell membranes
ISBN : 1071603736

Get Book

Expression, Purification, and Structural Biology of Membrane Proteins by Camilo Perez,Timm Maier Pdf

This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.

Pichia Protocols

James M Cregg
Pichia Protocols Book PDF

Author : James M Cregg
Publisher : Springer Science & Business Media
Page : 553 pages
File Size : 40,8 Mb
Release : 2007-08-08
Category : Science
ISBN : 9781588294296

Get Book

Pichia Protocols by James M Cregg Pdf

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.

Plant ABC Transporters

Markus Geisler
Plant ABC Transporters Book PDF

Author : Markus Geisler
Publisher : Springer
Page : 333 pages
File Size : 54,9 Mb
Release : 2014-09-06
Category : Science
ISBN : 9783319065113

Get Book

Plant ABC Transporters by Markus Geisler Pdf

This book is devoted to the fascinating superfamily of plant ATP-binding cassette (ABC) transporters and their variety of transported substrates. It highlights their exciting biological functions, covering aspects ranging from cellular detoxification, through development, to symbiosis and defense. Moreover, it also includes a number of chapters that center on ABC transporters from non-Arabidopsis species. ABC proteins are ubiquitous, membrane-intrinsic transporters that catalyze the primary (ATP-dependent) movement of their substrates through biological membranes. Initially identified as an essential aspect of a vacuolar detoxification process, genetic work in the last decade has revealed an unexpectedly diverse variety of ABC transporter substrates, which include not only xenobiotic conjugates, but also heavy metals, lipids, terpenoids, lignols, alkaloids and organic acids. The discovery that members of the ABCB and ABCG family are involved in the movement of phytohormones has further sparked their exploration and provided a new understanding of the whole family. Accordingly, the trafficking, regulation and structure-function of ABCB-type auxin transporters are especially emphasized in this book.

Membrane Protein Crystallization

Anonim
Membrane Protein Crystallization Book PDF

Author : Anonim
Publisher : Academic Press
Page : 336 pages
File Size : 52,8 Mb
Release : 2009-05-29
Category : Science
ISBN : 0080961592

Get Book

Membrane Protein Crystallization by Anonim Pdf

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Novel Frontiers in the Production of Compounds for Biomedical Use

A. van Broekhoven,Fred Shapiro,Jozef Anné
Novel Frontiers in the Production of Compounds for Biomedical Use Book PDF

Author : A. van Broekhoven,Fred Shapiro,Jozef Anné
Publisher : Springer Science & Business Media
Page : 436 pages
File Size : 49,8 Mb
Release : 2006-04-11
Category : Technology & Engineering
ISBN : 9780306468858

Get Book

Novel Frontiers in the Production of Compounds for Biomedical Use by A. van Broekhoven,Fred Shapiro,Jozef Anné Pdf

The present book entitled “Novel Frontiers in the Production of Compounds for Biomedical Uses” can perhaps be placed in its best perspective by the Shakespearean character in The Tempest who exclaimed" What’s past is prologue”. Indeed, this compilation of some of the outstanding presentations in the field of biomedicine made at th the 9 European Congress on Biotechnology (Brussels, Belgium, July 11-15, 1999) not only reflects the achievements of the recent past, but provides a privileged glimpse of the biotechnology that is emerging in the first decade of the new Millennium. It is becoming increasingly apparent that biotechnology is offering biomedicine novel approaches and solutions to develop a sorely needed new generation of biopharmaceuticals. This is all the more necessary because in recent years, new diseases have emerged with extraordinary lethality in all corners of the globe, while age-related chronic illnesses have filled the gap wherever biomedicine has made successful inroads. The rise of antibiotic resistance also poses major threats to public health. Thus, as disease patterns evolve, the rational development of new drugs is becoming urgent, not only for the clinical outcome of patients, but also in optimising the allocation of scarce health care resources through the use of cost-effective productions methods. It is in response to all these challenges that biotechnology offers new strategies that go beyond the more traditional approaches. By the mid-1990’s, the number of recombinant products approved annually for therapeutic use reached double digits. With the advent of the genomics revolution.

Cell-Free Protein Expression

W. Antoni Kudlicki
Cell Free Protein Expression Book PDF

Author : W. Antoni Kudlicki
Publisher : CRC Press
Page : 242 pages
File Size : 55,6 Mb
Release : 2007-11-27
Category : Science
ISBN : 9781498713290

Get Book

Cell-Free Protein Expression by W. Antoni Kudlicki Pdf

Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica