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Homing Endonucleases and Inteins by Marlene Belfort,Barry L. Stoddard,David W. Wood,Vicky Derbyshire Pdf
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
Hyperthermophilic Enzymes by Michael W. W. Adams,Robert M. Kelly Pdf
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 330 and 331) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include redox and thiol-dependent proteins, nucleic acid modifying enzymes, and protein stability from biochemical and biophysical standpoints.
Physiology and Genetics by Timm Anke,Anja Schüffler Pdf
In the last few decades, DNA-based tools for the investigation of fungal taxonomy, signal transduction and regulation, differentiation processes and biosynthetic potential have accelerated advances in our understanding of the Mycota. This completely updated and revised second edition presents a selection of exciting issues involving basic and applied aspects of fungal physiology and genetics. In 14 chapters, respected experts provide an overview of traditional, topical and future aspects of basic fungal principles and potential applications in biotechnology. The contributions will bring scientists up-to-date on the latest developments, and help students familiarize themselves with the different topics.
This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in Split Inteins: Methods and Protocols explore new techniques on how to use split inteins for affinity purification of overproduced proteins, and split-intein based technologies to prepare cyclic peptides and proteins. The next few chapters discuss semisynthetic protein trans-splicing using one synthetic intein piece, synthetic intein-extein pieces used to deliver other cargos for chemical modification both of purified proteins and of proteins in living cells, as well as isotopic labeling of proteins for NMR studies, and a discussion on how protein block copolymers can be generated by protein trans-splicing to form protein hydrogels. The last few chapters deal with intein applications in transgenic plants and conditional inteins that can be regulated in artificial ways by small molecules or light, a cassette-based approach to quickly test many intein insertion positions, and a computational approach to predict new intein split sites (the approach also works for other proteins). Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Split Inteins: Methods and Protocols is a valuable resource that will provide guidance toward possibilities of split intein applications, explore proven and detailed protocols adaptable to various research projects, and inspire new method developments.
Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4).
Lewin's GENES XII by Jocelyn E. Krebs,Elliott S. Goldstein,Stephen T. Kilpatrick Pdf
Now in its twelfth edition, Lewin's GENES continues to lead with new information and cutting-edge developments, covering gene structure, sequencing, organization, and expression. Leading scientists provide revisions and updates in their individual field of study offering readers current data and information on the rapidly changing subjects in molecular biology.
Lewin's Essential GENES by Jocelyn E. Krebs,Elliott S. Goldstein,Stephen T. Kilpatrick Pdf
Extensively reorganized and revised with the latest data from this rapidly changing field, Lewin's Essential GENES, Third Edition, provides students with a comprehensive overview of molecular biology and molecular genetics.
Esterases—Advances in Research and Application: 2012 Edition by Anonim Pdf
Esterases—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Esterases. The editors have built Esterases—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Esterases in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Esterases—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
In this book, the author Joseph G. Sinkovics liberally shares his views on the cancer cell which he has been observing in vivo and in vitro, over a life time. Readers will learn how, as an inherent faculty of the RNA/DNA complex, the primordial cell survival pathways are endogenously reactivated in an amplified or constitutive manner in the multicellular host, and are either masquerading as self-elements or as placentas, to which the multicellular host is evolutionarily trained to extend full support. The host obliges. The author explains that there is no such evidence that “malignantly transformed” human cells survive in nature. However, when cared for in the laboratory, these cells live and replicate as immortalized cultures. These cells retain their vitality upon storage in liquid nitrogen. One can only imagine an astrophysical environment in which such cells could survive; perhaps, first their seemingly humble exosomes would populate that environment. Immortal cell populations so created may survive as individuals, or may even re-organize themselves into multicellular colonies, as representatives of life for the duration of the Universe. This thought-provoking book is the work of a disciplined investigator and clinician with an impeccable reputation, and he enters a territory that very few if any before him have approached from the same angles. It will appeal to researchers with an interest in cell survival pathways and those researching cancer cells.
The Dictionary of Genomics, Transcriptomics and Proteomics, 4 Volume Set by Guenter Kahl Pdf
Now in its fifth edition and for the first time available as an electronic product with all entries cross-linked. This very successful long-seller has once again been thoroughly updated and greatly expanded. It now contains over 13,000 entries, and comprehensively covering genomics, transcriptomics, and proteomics. Each entry contains an extensive explanation, including a comprehensive listing of synonyms and acronyms, and all formulas have been redrawn to create a uniform style, while most of the figures are custom designed for this dictionary. The ultimate reference for all terms in the -omics fields.
Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the
Lewin's GENES X by Jocelyn E. Krebs,Elliott S. Goldstein,Stephen T. Kilpatrick Pdf
Lewin's GENES X features informative new chapters, as well as a reorganization of material, provides a more logical flow of topics and many chapters have been renamed to better indicate their contents. Lewin's GENES X also contains new pedagogical features to help students learn as they read and an online student study guide allows students to test themselves on key material. Important Notice: The digital edition of this book is missing some of the images or content found in the physical edition.
Autologous and Cancer Stem Cell Gene Therapy by Roger Bertolotti,Keiya Ozawa Pdf
Stem cells provide for life-long cell replacement in tissues and organs, and have inherent homing abilities that are critical in therapeutic applications. Stem cells are also the driving force of cancer where genetic/epigenetic alterations culminate in tumorigenesis either in tissue stem cells or in some of their derivatives. As a rare subset of the tumor, cancer stem cells are the only drive of tumor initiation/propagation. Autologous and cancer stem cells are thus the key targets of 1) long-term and transient-regenerative/epigenetic gene therapy and 2) of recurrence-free anticancer therapy, respectively. While cancer stem cell gene therapy still needs time to accomplish, autologous stem cells have been instrumental in the first unequivocal successes for gene therapy whereby ex vivo retrovirally corrected hematopoietic stem cells have been returned to the patients. This timely book presents 1) the aforementioned stem cell gene therapy achievements that rely on random-integration of therapeutic transgenes into host chromosomes and 2) emerging experimental approaches aimed at eliminating random-integration oncogenic hazards through site-specific integration or gene targeting. Breakthrough endonuclease-boosted gene targeting for gene correction (inherited diseases) or targeted integration of therapeutic transgenes (other disorders) culminating in an efficiency compatible with clinical trials is one of the highlights of the book. Highlights also include the pioneering transplantation of adult pluripotent stem cells as a substitute for tissue-specific stem cells, thereby pinpointing the invaluable potential for stem cell gene therapy applications of autologous cells able to contribute to all three germ layers. Stem cell gene therapy is thus discussed in terms of 1) magnifying stem cell therapeutic homing through transient regenerative gene therapy and 2) of tackling most pathologies (including mitochondrial DNA diseases and ageing disorders) through stem cell repopulation dynamics into appropriate niches (long-term engraftment) and tissues (cell turn-over). Regarding cancer stem cell gene therapy, focus is on both the increasing number of identified tissue-specific cancer stem cells as the ultimate therapeutic targets and on the development of armed stem cells as tumor-homing vectors for targeted anticancer therapy. Sample Chapter(s). Introduction: Toward a Universal Platform for Autologous Stem Cell Gene Therapy (335 KB). Chapter 1: Stem Cell Gene Therapy for ADA-Deficiency without Myelopreparative Conditioning (318 KB). Contents: Introduction: Toward a Universal Platform for Autologous Stem Cell Gene Therapy; Long-term Stem Cell Gene Therapy: From Current Clinical Random-Integration Achievements to Stem Cell Gene Targeting/Cybridization: From Pioneering Clinical Retroviral Gene Therapy to Experimental Site-Specific Integrative Gene Therapy; Stem Cell Gene Targeting/Cybridization: Toward Endonuclease-Boosted Gene Repair/Alteration, Custom Site-Specific Integrative Gene Therapy and Transmitochondrial Therapy/Rejuvenation; Adult Pluripotent Stem Cells: Emerging Stem Cell/Gene Therapy Breakthrough; Cancer Stem Cell Gene Therapy: Cancer Stem Cells as Breakthrough Targets of Cancer Gene Therapy; Armed Stem Cells as Tumor-Homing Vectors for Cancer Gene Therapy. Readership: Clinicians, scientists, physicians, veterinarians, dentists, pharmacists/chemists, interested in an ongoing medical/veterinarian breakthrough that is expected to revolutionize the practice of Medicine/Dentistry and that relies on cell biology, molecular biology/genetics, developmental biology, biochemistry, molecular pathology and animal science.
Comprehensive Biotechnology by KG Ramawat | Shaily Goyal Pdf
For B.Sc. and M.Sc. Students of Different Indian Universities as per UGC Model Curriculum. This is revised edition of the book "Plant Biotechnology". Several new topics such as Aquporins, Artificial intelligence Automation in Micropropagation, Biochips, Green House, Hydroponic, Inteins, Nanotechnology, Space Biotechnology, Supercritical Fluid extraction, etc. have been included in this revised. This edition provides latest information on the frontier area of biotechnology.
