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Author : J. Tampion,M. D. Tampion
Publisher : Cambridge University Press
Page : 268 pages
File Size : 51,6 Mb
Release : 1987-12-10
Category : Medical
ISBN : 0521255562
This 1987 book gives a coherent overview of preparation and uses of immobilized enzymes.
Author : Ichiro Chibata,Lemuel B. Wingard
Publisher : Elsevier
Page : 372 pages
File Size : 55,7 Mb
Release : 2014-06-28
Category : Science
ISBN : 9781483215860
Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Author : R.M. Buitelaar,C. Bucke,J. Tramper,R.H. Wijffels
Publisher : Elsevier
Page : 865 pages
File Size : 48,7 Mb
Release : 1996-03-21
Category : Science
ISBN : 9780080534473
This publication contains full papers of both oral and poster presentations of the symposium "Immobilized Cells: Basics and Applications" that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. This volume covers recent developments in the field of immobilization e.g.: new support materials, characterization of support materials, kinetic characterizations, dynamic modelling, bioreactor types, scale up and applications are also given. Applications in the field of medicine, fermentation technology, food technology and environmental technology are described. Guidelines for research with immobilized cells. Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given. The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. The manuscripts presented in these proceedings give an extensive and recent overview of the research and applications of immobilized-cell technology.
Author : Colin R. Phillips,Yiu Cheong Poon
Publisher : Springer Science & Business Media
Page : 171 pages
File Size : 48,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642732492
Growth of immobilized cells can be viewed as an alternative to growth of free cells in many instances. In others, immobilization confers a precision of control over the process not possible in free growth. Immobilization of cells can sometimes be considered to be a lower cost alternative to immobilization of enzymes. In this volume, immobilization procedures based on mechanical means and bonding of various types are examined, with detailed application examples. These applications include microorganisms, plant and animal cells, sub-cellular organelles and multiple enzyme systems. Particular attention is devoted to enzyme properties in immobilized cells and the properties of the carrier. The volume should provide the reader with a comprehensive overview of the subject, together with copious references. As well as serving as a research monograph, it could be used to provide reference material for a graduate course. Special thanks are due Mrs. JENNIFER KERBY for her dedicated work in the preparation of the manuscript, and IT-CHIN HSIEH for bibliographical assistance. COLIN R. PHILLIPS Toronto, July 1988 YIU C. POON v Table of Contents 1 Introduction. 1 References . 9 2 Methods of Cell Immobilization 11 2.1 Mechanical Immobilization . 11 2.1.1 Mycelial Pellet and Mat 11 2.1.2 Encapsulation .. 48 2.1.3 Dialysis Culture. . . 49 2.1.4 Entrapment. .... 50 2.2 Covalent Attachment 61 2.3 Ionic Attachment 62 2.3.1 Flocculation 62 2.3.2 Adsorption . 64 References . 66 3 Special Problems and Extended Applications . 75 3.1 Special Problems and Techniques .
Author : Jose M. Guisan
Publisher : Humana
Page : 0 pages
File Size : 48,7 Mb
Release : 2016-08-27
Category : Science
ISBN : 1493959883
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Author : Allen I. Laskin
Publisher : Butterworth-Heinemann
Page : 344 pages
File Size : 52,6 Mb
Release : 1985
Category : Science
ISBN : UOM:39015009827208
Author : Jose M. Guisan,Juan M. Bolivar,Fernando López-Gallego,Javier Rocha-Martín
Publisher : Humana
Page : 495 pages
File Size : 53,8 Mb
Release : 2021-01-29
Category : Science
ISBN : 1071602179
This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Author : Viktor Nedovic,Ronnie Willaert
Publisher : Springer Science & Business Media
Page : 542 pages
File Size : 40,8 Mb
Release : 2013-04-17
Category : Science
ISBN : 9789401716383
Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.
Author : Winfried Hartmeier
Publisher : Springer Science & Business Media
Page : 217 pages
File Size : 52,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642733642
Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.
Author : R. D. Tyagi,Kannan Vembu
Publisher : CRC Press
Page : 294 pages
File Size : 53,9 Mb
Release : 1990-05-23
Category : Technology & Engineering
ISBN : 0849351766
The purpose of this state-of-the-art publication is to provide up-to-date and pertinent scientific information concerning immobilized cell processes for treatment of wastewater. It comprehensively reviews and examines essential data on the feasibility of various immobilization methods, with special reference to wastewater treatment. It also discusses methods of identification of structure and composition of microbial aggregates and analytical methods for the estimation of biomass in the presence of carriers. This work reports the novel process of immobilized microalgae and cyanobacteria for wastewater treatment, while highlighting their future prospects. Additionally, it critically reviews the various fixed film bioreactor configurations. This book is a must for all engineers, planners, scientists, students and sewage treatment plant operators.
Author : Bo Mattiasson
Publisher : CRC Press
Page : 254 pages
File Size : 50,8 Mb
Release : 2019-08-08
Category : Science
ISBN : 9781351090285
Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.
Author : Mattiasson
Publisher : CRC Press
Page : 151 pages
File Size : 44,6 Mb
Release : 2018-01-18
Category : Science
ISBN : 9781351081849
Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.
Author : Colin Webb,George A. Dervakos
Publisher : Academic Press
Page : 220 pages
File Size : 47,9 Mb
Release : 1996-02-09
Category : Medical
ISBN : STANFORD:36105018451802
This book provides a coherent analysis of viable cell immobilization, an emerging technology spanning a number of disciplines. Using detailed examples drawn from a variety of commerically-relevant large-scale fermentation reactions, the book covers both the practical and theoretical aspects of this technology. The authors discuss progress in the design of immobilized cell reactors, emphasizing mixing, fluidization characteristics and carrier stability. Mathematical models and results from computer simulations describe the dynamic behavior of viable cells and their intra-particle distribution, including micro-colony formation. As an aid to the reader, the authors have included examples drawn from their own practical research into biomass support particle (BSP) technology. Key Features * Provides a critical re-appraisal of already available material and sets new information in an historical context * Addressses both the theoretical and practical aspects of the technology * Includes a practical discussion of technology and its merits * Disseminates results from biomass support particle (BSP) technology
Author : Rene H. Wijffels
Publisher : Springer Science & Business Media
Page : 270 pages
File Size : 52,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642568916
used. Generally in the development of a process, research activity will start with the physiology of the immobilized cells and be concluded by scale-up and application. Guidelines for the different phases in research are given below. Springer Lab Manual about immobilized cells The Springer Lab Manual about immobilized cells is structured in such a way that guidelines that resulted from the conference 'Immobilized Cells: Basics and Applications' were followed and translated into some practical methods. As for the guidelines for immobilized cells a structure was chosen in which general aspects of immobilization were covered, the kinetics were described and engineering aspects were covered. Besides laboratory meth ods some simple calculation procedures have been given in this lab man ual. The combination of the practical methods with the calculations pro vide a tool for carrying out research with immobilized cells. The lab manual concludes with some recent, promising and practical cases of immobilized cells as examples of applications with potential.
Author : Bo Mattiasson
Publisher : CRC Press
Page : 167 pages
File Size : 54,9 Mb
Release : 2019-08-08
Category : Science
ISBN : 9781351081832
Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.
