Immunohistochemical Expression

Author : Rosario Caltabiano,Carla Loreto
Publisher : MDPI
Page : 168 pages
File Size : 51,7 Mb
Release : 2021-09-02
Category : Medical
ISBN : 9783036504001

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Immunohistochemical Expression by Rosario Caltabiano,Carla Loreto Pdf

Immunohistochemistry (IHC) is an ancillary method, widely used in pathologists’ practice, that allows identifying diagnostic and prognostic/predictive of therapeutic response protein markers on tissue samples by the use of specific monoclonal antibodies and chromogenic substances that guarantee the visualization of an antibody–antigene binding complex under a light microscope [1]. Coon et al., in 1941 [2], first introduced the use of fluorochrome-conjugated antibodies in clinical practice. Since then, IHC has gone from being a useful tool for identifying the differentiation line of otherwise undifferentiated cells to a technique capable of providing not only diagnostic but also prognostic and predictive indications of responses to specific therapeutic options [1,3]. The abovementioned peculiarities have made IHC one of the most used ancillary methods in the histopathological approach to human neoplastic and non-neoplastic diseases [3-5]. This Special Issue contains 11 accepted papers that provide readers with a comprehensive update on current and future applications of IHC in medical practice.

Author : Muin S.A. Tuffaha,Hans Guski,Glen Kristiansen
Publisher : Springer Nature
Page : 373 pages
File Size : 48,5 Mb
Release : 2024-01-17
Category : Medical
ISBN : 9783031450242

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Immunohistochemistry in Tumor Diagnostics by Muin S.A. Tuffaha,Hans Guski,Glen Kristiansen Pdf

This new and updated edition provides an extensive overview of the antibodies employed in diagnostic tumor histopathology, and it presents concise summaries of the immunoprofiles of most tumors. Additionally, it offers practical diagnostic algorithms and invaluable tips to facilitate the interpretation of results. The work includes the newest available antibodies and the most recent 5th edition of the WHO classification of tumors. Furthermore, a chapter on biomarkers for theranostic applications has been added to enhance the book's utility. Designed as a practical and user-friendly bench reference for diagnostic tumor histopathology, this book is recommended to histopathologists of all levels, from aspiring residents to seasoned specialists seeking a reliable and comprehensive resource for tumor diagnostics. Moreover, it is highly recommended for oncologists, hematologists, and researchers involved in these fields.

Author : Rosario Caltabiano,Carla Loreto
Publisher : Unknown
Page : 168 pages
File Size : 46,5 Mb
Release : 2021
Category : Electronic
ISBN : 303650401X

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Immunohistochemical Expression by Rosario Caltabiano,Carla Loreto Pdf

Immunohistochemistry (IHC) is an ancillary method, widely used in pathologists' practice, that allows identifying diagnostic and prognostic/predictive of therapeutic response protein markers on tissue samples by the use of specific monoclonal antibodies and chromogenic substances that guarantee the visualization of an antibody-antigene binding complex under a light microscope [1]. Coon et al., in 1941 [2], first introduced the use of fluorochrome-conjugated antibodies in clinical practice. Since then, IHC has gone from being a useful tool for identifying the differentiation line of otherwise undifferentiated cells to a technique capable of providing not only diagnostic but also prognostic and predictive indications of responses to specific therapeutic options [1,3]. The abovementioned peculiarities have made IHC one of the most used ancillary methods in the histopathological approach to human neoplastic and non-neoplastic diseases [3-5]. This Special Issue contains 11 accepted papers that provide readers with a comprehensive update on current and future applications of IHC in medical practice.

Author : David J Dabbs
Publisher : Elsevier Health Sciences
Page : 992 pages
File Size : 46,7 Mb
Release : 2017-11-29
Category : Medical
ISBN : 9780323551601

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Diagnostic Immunohistochemistry E-Book by David J Dabbs Pdf

User-friendly and concise, the new edition of this popular reference is your #1 guide for the appropriate use of immunohistochemical stains. Dr. David J. Dabbs and leading experts in the field use a consistent, organ system approach to cover all aspects of the field, with an emphasis on the role of genomics in diagnosis and theranostic applications that will better inform treatment options. Each well-written and well-researched chapter is enhanced with diagnostic algorithms, charts, tables, and superb, full-color histologic images, making this text a practical daily resource for all surgical pathologists. Features a systematic approach to the diagnostic entities of each organ system, including detailed differential diagnoses, diagnostic algorithms, and immunohistograms that depict immunostaining patterns of tumors. Covers many more antigens than other texts, and discusses antibody specifications with tables that convey information on uses, clones, vendors, sources, antibody titers, and types of antigen retrieval. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Contains new material on non-lymphoid malignancies, Hodgkin/non-Hodgkin lymphoma, and an expanded chapter on digital imaging and quantilative immunohistochemistry. Provides new grading schemes for several organs, along with new antibodies to cover more genomic immunohistochemistry applications. Offers more emphasis in the breast section of "eyes on" tissue for molecular/IHC prognostics compared to the current trend of gene-expression profiling of breast cancer.

Author : Muin S.A. Tuffaha,Hans Guski,Glen Kristiansen
Publisher : Springer
Page : 264 pages
File Size : 54,7 Mb
Release : 2017-09-25
Category : Medical
ISBN : 9783319535777

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Immunohistochemistry in Tumor Diagnostics by Muin S.A. Tuffaha,Hans Guski,Glen Kristiansen Pdf

This book offers a comprehensive yet concise overview of immunoprofile of tumors and antibodies used in contemporary surgical pathology, and provides diagnostic algorithms for approaching tumor diagnostics. Immunohistochemistry has become the most important ancillary technique in diagnostic pathology in the last 20 years, and unlike most books on tumor diagnostics, this volume discusses in details immunohistochemical biomarkers, diagnostic approaches and their pitfalls, as well as the immunoprofile of common tumors throughout all systems of human body. With numerous color figures and detailed flowcharts, it appeals to all pathologists be they young residents in training who want a brief introduction to this technique, or specialists in need of a reliable and comprehensive reference resource in tumors diagnostics.

Author : Fan Lin,Jeffrey Prichard
Publisher : Springer
Page : 764 pages
File Size : 55,6 Mb
Release : 2015-02-21
Category : Medical
ISBN : 9781493915781

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Handbook of Practical Immunohistochemistry by Fan Lin,Jeffrey Prichard Pdf

In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

Author : Peiguo Chu,Peiguo G. Chu,Lawrence Weiss
Publisher : Cambridge University Press
Page : 713 pages
File Size : 50,9 Mb
Release : 2009-04-27
Category : Medical
ISBN : 9780521874304

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Modern Immunohistochemistry by Peiguo Chu,Peiguo G. Chu,Lawrence Weiss Pdf

This book offers a thorough overview of many diagnostic tools used in immunohistochemistry, supplemented with more than 600 color photomicrographs.

Author : Peiguo Chu,Lawrence Weiss
Publisher : Cambridge University Press
Page : 523 pages
File Size : 54,6 Mb
Release : 2014-02-27
Category : Medical
ISBN : 9781107040151

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Modern Immunohistochemistry with DVD-ROM by Peiguo Chu,Lawrence Weiss Pdf

Fully updated to reflect the latest developments in the field, this best-selling practical guide offers concise text, summary tables and high-quality images. An essential text for residents, this is also an extremely valuable resource for practitioners in anatomic pathology wishing to familiarise themselves with diagnostic markers at a quick glance.

Author : Fan Lin,Jeffrey Prichard
Publisher : Springer Science & Business Media
Page : 616 pages
File Size : 50,7 Mb
Release : 2011-07-01
Category : Medical
ISBN : 9781441980625

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Handbook of Practical Immunohistochemistry by Fan Lin,Jeffrey Prichard Pdf

In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

Author : Gerard J. Nuovo
Publisher : Academic Press
Page : 298 pages
File Size : 47,9 Mb
Release : 2013-02-07
Category : Science
ISBN : 9780124160019

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In Situ Molecular Pathology and Co-Expression Analyses by Gerard J. Nuovo Pdf

In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework. Includes over 200 easy-to-follow experimental protocols Features chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the field Offers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses

Author : Mahmoud Lotfy
Publisher : BoD – Books on Demand
Page : 370 pages
File Size : 44,8 Mb
Release : 2011-06-15
Category : Medical
ISBN : 9789533074122

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Gastric Carcinoma by Mahmoud Lotfy Pdf

Gastric cancer is one of the most common tumors worldwide. It has a heterogeneous milieu, where the genetic background, tumor immunology, oxidative stress, and microbial infections are key players in the multiple stages of tumorigenesis. These diverse factors are linked to the prognosis of the gastric cancer and the survival of gastric cancer patients. This book is appropriate for scientists and students in the field of oncology, gastroenterology, molecular biology, immunology, cell biology, biology, biochemistry, and pathology. This authoritative text carefully explains the fundamentals, providing a general overview of the principles followed by more detailed explanations of these recent topics efficiently. The topics presented herein contain the most recent knowledge in gastric cancer concerning the oncogenic signaling, genetic instability, the epigenetic aspect, molecular features and their clinical implications, miRNAs, integrin and E-cadherin, carbohydrate-associated-transferases, free radicals, immune cell responses, mucins, Helicobacter-pylori, neoadjuvant and adjuvant therapy, prophylactic strategy for peritoneal recurrence, and hepatic metastasis.

Author : David J. Dabbs
Publisher : Saunders
Page : 972 pages
File Size : 40,7 Mb
Release : 2010
Category : Medical
ISBN : CHI:092591500

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Diagnostic Immunohistochemistry by David J. Dabbs Pdf

Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin's lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. The fully searchable text is also available online at expertconsult.com, along with a downloadable image bank and access to Path Consult. This concise and complete resource is today's indispensable guide to the effective use of immunohistochemical diagnosis. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Presents chapters arranged by organ system for comprehensive coverage of all relevant information in a convenient and intuitive organization. Provides quick reference graphs for antibodies throughout the text that illustrate the frequency of immunostaining for a variety of antibodies in tumors. Includes Key Diagnostic Points boxes in every chapter for a quick summary of text areas that are of particular importance. Features an expert author for each chapter to ensure coverage of the current state of the art. Includes access to the full text online at expertconsult.com, along with a downloadable image bank and Path Consult. Provides guidance on the role of genomics in identifying genetic and molecular aspects of disease that may affect patient care and therapeutic approaches. Covers theranostic applications to enable you to evaluate therapeutic choices based on immunohistochemical results. Reflects the latest developments in the field through new chapters on molecular anatomic pathology and immunocytology, as well as updated chapters on immunohistology of the prostate, bladder, testis, and kidney and Non-Hodgkin's lymphoma. Discusses antibody specifications with tables that convey information on uses, clones, vendors, sources, antibody titers, and types of antigen retrieval. Presents key differential diagnoses boxes that provide tabular summaries of DDx and algorithms. Features discussions of ancillary anatomic molecular diagnostics as an adjunct to immunohistochemistry for a more well-rounded diagnostic approach.

Author : Emily Stuchfield-Denby
Publisher : Unknown
Page : 0 pages
File Size : 46,9 Mb
Release : 2022
Category : Electronic
ISBN : OCLC:1417601568

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Immunohistochemical Expression of DNAJB9 in IgG4-related Disease by Emily Stuchfield-Denby Pdf

Introduction. Immunoglobulin G4-related disease (IgG4-RD) is a rare immune-mediated condition with expansion of plasmablasts/plasma cells and possible upregulation of DNAJB9. DNAJB9 (a co-chaperone protein that governs the integrity and functions of cells) was described as a specific marker for fibrillary glomerulonephritis, a rare proliferative form of glomerulonephritis displaying histological similarities with IgG4-related kidney disease. DNAJB9 may be a putative autoantigen. We aimed to study the immunohistochemical expression of DNAJB9 in IgG4-RD to explore its role in disease pathophysiology. Methods. We selected patients with definite IgG4-RD (RCD, ACR/EULAR or ICDC classification criteria), non-auto-immune (NAI) controls and Sjögren's syndrome controls, and produced tissue slides for anti-DNAJB9 immunohistochemistry on which we performed double reading and applied our intensity scoring system ranging from 0 to 4. Our positive control was a renal sample suggestive of fibrillary glomerulonephritis. Results. We studied the tissue slides of 9 IgG4-RD patients and 15 NAI controls (including salivary gland, pancreatic, pulmonary, pleural, and retroperitoneal fibrotic tissue). Median intensity for endothelial cells was 0 in IgG4-RD and 2 in NAI controls (p

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 49,7 Mb
Release : 2024-06-28
Category : Electronic
ISBN : 9789814472371

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Metallothioneins in Biochemistry and Pathology by Anonim Pdf

Author : Satish Tickoo,Ying-bei Chen,Debra Zynger
Publisher : Lippincott Williams & Wilkins
Page : 368 pages
File Size : 55,6 Mb
Release : 2015-07-10
Category : Medical
ISBN : 9781469830520

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Biopsy Interpretation of the Kidney & Adrenal Gland by Satish Tickoo,Ying-bei Chen,Debra Zynger Pdf

This practical volume in the Biopsy Interpretation Series is your guide to effective diagnostic biopsy of the kidney and adrenal glands. Presented in a reader-friendly format, the text includes abundant photomicrographs of actual biopsy samples as well as correlative photos of surgical specimens and ancillary immunohistochemical studies. Each organ is addressed in a separate section that includes an introduction to biopsy techniques and an overview of normal histology. Positive diagnoses for renal lesions are described by tumor type. Adrenal lesions are grouped by primary and metastatic tumors, reflecting the main diagnostic considerations at the adrenal site. Uniquely detailed focus addresses the specific techniques and interpretive features of renal and adrenal biopsy. Timely content addresses the use of renal biopsy in minimally invasive therapeutic techniques as well as its role in active surveillance of renal masses. The latest ISUP classifications reflect newly described renal tumors discussed at the March 2012 USCAP annual meeting. Clinical implications of each diagnosis are highlighted throughout the text to guide therapeutic decision-making.