In Vitro

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In Vitro Fertilization

Author : Zsolt Peter Nagy,Alex C. Varghese,Ashok Agarwal
Publisher : Springer
Page : 962 pages
File Size : 51,5 Mb
Release : 2019-07-03
Category : Health & Fitness
ISBN : 9783319430119

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In Vitro Fertilization by Zsolt Peter Nagy,Alex C. Varghese,Ashok Agarwal Pdf

Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.

The Human Embryo In Vitro

Author : Catriona A. W. McMillan
Publisher : Cambridge University Press
Page : 249 pages
File Size : 44,9 Mb
Release : 2021-04-01
Category : Law
ISBN : 9781108945165

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The Human Embryo In Vitro by Catriona A. W. McMillan Pdf

The Human Embryo in vitro explores the ways in which UK law engages with embryonic processes under the Human Fertilisation and Embryology Act 1990 (as amended), the intellectual basis of which has not been reconsidered for almost thirty years. McMillan argues that in regulating 'the embryo' – that is, a processual liminal entity in itself - the law is regulating for uncertainty. This book offers a fuller understanding of how complex biological processes of development and growth can be better aligned with a legal framework that purports to pay respect to the embryo while also allowing its destruction. To do so it employs an anthropological concept, liminality, which is itself concerned with revealing the dynamics of process. The implications of this for contemporary regulation of artificial reproduction are fully explored, and recommendations are offered for international regimes on how they can better align biological reality with social policy and law.

In Vitro

Author : William Roy
Publisher : Humanoids, Inc.
Page : 158 pages
File Size : 49,7 Mb
Release : 2020-03-31
Category : Comics & Graphic Novels
ISBN : 9781643376745

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In Vitro by William Roy Pdf

A newly wedded couple is full of life, love, and inspiration. They have everything they've always wanted...except a child.

Individualized In-Vitro Fertilization

Author : Human M. Fatemi,Barbara Lawrenz
Publisher : Cambridge University Press
Page : 149 pages
File Size : 50,5 Mb
Release : 2021-03-04
Category : Medical
ISBN : 9781108799683

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Individualized In-Vitro Fertilization by Human M. Fatemi,Barbara Lawrenz Pdf

This book provides updates on the most recent developments in individualized infertility treatment resulting in improved outcomes.

In Vitro Methods in Pharmaceutical Research

Author : Jose V. Castell,Maria Jose Gmez-Lechn
Publisher : Elsevier
Page : 483 pages
File Size : 55,7 Mb
Release : 1996-10-04
Category : Medical
ISBN : 9780080534602

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In Vitro Methods in Pharmaceutical Research by Jose V. Castell,Maria Jose Gmez-Lechn Pdf

In Vitro Methods in Pharmaceutical Research provides a comprehensive guide to laboratory techniques for evaluating in vitro organ toxicity using cellular models. Step-by-step practical tips on how to perform and interpret assays for drug metabolism and toxicity assessment are provided, along with a comparison of different techniques available. It is a welcome addition to the literature at a time when interest is growing in cellular in vitro models for toxicology and pharmacology studies. Meets the continuing demand for information in this field Compares In Vitro techniques with other methods Describes cell-culture methods used to investigate toxicity in cells derived from different organs Includes contributions by leading experts in the field

Cellular In Vitro Testing

Author : John Haycock,Arti Ahluwalia,J. Malcolm Wilkinson
Publisher : CRC Press
Page : 192 pages
File Size : 53,6 Mb
Release : 2014-08-27
Category : Medical
ISBN : 9789814364980

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Cellular In Vitro Testing by John Haycock,Arti Ahluwalia,J. Malcolm Wilkinson Pdf

Growing cells in 2D under static conditions has long been the gold standard of cell culture, despite this method not being representative of the complex in vivo environment. The use of animal models also has clear ethical and scientific limitations, and increasingly the 3Rs (replacement, refinement, reduction) in relation to animal models are being integrated into the modern-day scientific practice. Focusing on new 3D in vitro methods now available to researchers, this book brings together examples of leading-edge work being conducted internationally for improving in vitro cell culture methods, in particular the use of systems for enabling cell culture under laminar flow and the use of 3D scaffolds for providing cells with a structure which replicates the function of the extracellular matrix and encouraging interactions more akin to an in vivo environment.

Monoclonal Antibody Production

Author : National Research Council,Institute for Laboratory Animal Research,Committee on Methods of Producing Monoclonal Antibodies
Publisher : National Academies Press
Page : 74 pages
File Size : 55,7 Mb
Release : 1999-06-06
Category : Medical
ISBN : 9780309064477

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Monoclonal Antibody Production by National Research Council,Institute for Laboratory Animal Research,Committee on Methods of Producing Monoclonal Antibodies Pdf

The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.

Assisted Reproductive Technology Surveillance

Author : Dmitry M. Kissin,G. David Adamson,Georgina Chambers,Christian De Geyter
Publisher : Cambridge University Press
Page : 255 pages
File Size : 50,5 Mb
Release : 2019-07-04
Category : Health & Fitness
ISBN : 9781108498586

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Assisted Reproductive Technology Surveillance by Dmitry M. Kissin,G. David Adamson,Georgina Chambers,Christian De Geyter Pdf

Offers a comprehensive guide to assisted reproductive technology surveillance, describing its history, global variations, and best practices.

In Vitro Culture of Trees

Author : J.M. Bonga,Patrick Aderkas
Publisher : Springer Science & Business Media
Page : 249 pages
File Size : 41,7 Mb
Release : 2013-06-29
Category : Nature
ISBN : 9789401580588

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In Vitro Culture of Trees by J.M. Bonga,Patrick Aderkas Pdf

Woody plants provide many challenges to the tissue culturist. Although there are many excellent tissue culture books and manuals available, these are generally strongly biased towards herbacious crops. Consequently, they often do not pay sufficient attention to the problems that specifically apply to in vitro culture of tree species. Culture of the latter often poses problems which are either absent or of lesser significance when culturing herbacious species. When trees in the field are used as explant source, the problems can be especially severe. For example, the physiological condition of the explants is difficult to control because of variation in weather and biotic factors. Furthermore, it is often difficult to obtain explants free of contaminants from field grown trees. Lack of genetic uniformity and maturation are additional problems one often has to deal with when culturing tree cells or tissues. These problems are emphasized in this text. In vitro culture of trees is not viewed in isolation. It is considered in conjunction with breeding, traditional cloning and other common tree improvement techniques. The text discusses theoretical as well as practical aspects of the in vitro culture of trees.

In Vitro Toxicology Systems

Author : Anna Bal-Price,Paul Jennings
Publisher : Humana
Page : 0 pages
File Size : 50,9 Mb
Release : 2014-05-01
Category : Medical
ISBN : 1493905201

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In Vitro Toxicology Systems by Anna Bal-Price,Paul Jennings Pdf

In Vitro Toxicology Systems brings together important issues and considerations needed in order to develop a workable, reliable, integrated testing strategy for the replacement of animals in toxicity testing regimes. This thorough volume includes sections on in vitro models for systemic organ toxicity, neurotoxicity, sensory organs, immunotoxicity and reproductive toxicity and addresses how stem cells may be used going forward. The book also tackles difficult areas of toxicology such as carcinogenicity and nanotoxicology, with additional chapters dedicated to kinetics, metabolism, and in vitro in vivo extrapolation. The book also addresses biological processes such as stress response pathways and mechanistic biomarkers and how these can be uncovered and measured using high content approaches. Reliable and authoritative, In Vitro Toxicology Systems will be of benefit not only to students, scientists and regulators working in the field of chemical safety assessment but also to a wider scientific audience.

OECD Series on Testing and Assessment Guidance Document on Good In Vitro Method Practices (GIVIMP)

Author : OECD
Publisher : OECD Publishing
Page : 172 pages
File Size : 53,5 Mb
Release : 2018-12-10
Category : Electronic
ISBN : 9789264304796

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OECD Series on Testing and Assessment Guidance Document on Good In Vitro Method Practices (GIVIMP) by OECD Pdf

In the past several decades, there has been a substantial increase in the availability of in vitro test methods for evaluating chemical safety in an international regulatory context. To foster confidence in in vitro alternatives to animal testing, the test methods and conditions under which ...

In Vitro Drug Release Testing of Special Dosage Forms

Author : Nikoletta Fotaki,Sandra Klein
Publisher : John Wiley & Sons
Page : 314 pages
File Size : 54,6 Mb
Release : 2019-10-11
Category : Science
ISBN : 9781118675830

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In Vitro Drug Release Testing of Special Dosage Forms by Nikoletta Fotaki,Sandra Klein Pdf

Guides readers on the proper use of in vitro drug release methodologies in order to evaluate the performance of special dosage forms In the last decade, the application of drug release testing has widened to a variety of novel/special dosage forms. In order to predict the in vivo behavior of such dosage forms, the design and development of the in vitro test methods need to take into account various aspects, including the dosage form design and the conditions at the site of application and the site of drug release. This unique book is the first to cover the field of in vitro release testing of special dosage forms in one volume. Featuring contributions from an international team of experts, it presents the state of the art of the use of in vitro drug release methodologies for assessing special dosage forms’ performances and describes the different techniques required for each one. In Vitro Drug Release Testing of Special Dosage Forms covers the in vitro release testing of: lipid based oral formulations; chewable oral drug products; injectables; drug eluting stents; inhalation products; transdermal formulations; topical formulations; vaginal and rectal delivery systems and ophthalmics. The book concludes with a look at regulatory aspects. Covers both oral and non-oral dosage forms Describes current regulatory conditions for in vitro drug release testing Features contributions from well respected global experts in dissolution testing In Vitro Drug Release Testing of Special Dosage Forms will find a place on the bookshelves of anyone working with special dosage forms, dissolution testing, drug formulation and delivery, pharmaceutics, and regulatory affairs.

In Vitro Culture of Higher Plants

Author : R.L.M Pierik
Publisher : Springer Science & Business Media
Page : 346 pages
File Size : 41,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401157506

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In Vitro Culture of Higher Plants by R.L.M Pierik Pdf

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

Test No. 487: In Vitro Mammalian Cell Micronucleus Test

Author : OECD
Publisher : OECD Publishing
Page : 23 pages
File Size : 55,5 Mb
Release : 2010-07-23
Category : Electronic
ISBN : 9789264091016

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Test No. 487: In Vitro Mammalian Cell Micronucleus Test by OECD Pdf

The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are ...

In Vitro Culture of Mycorrhizas

Author : Stéphane Declerck,D. G. Strullu,André Fortin
Publisher : Springer Science & Business Media
Page : 418 pages
File Size : 44,5 Mb
Release : 2005-04-13
Category : Nature
ISBN : 3540240276

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In Vitro Culture of Mycorrhizas by Stéphane Declerck,D. G. Strullu,André Fortin Pdf

This is the first book describing in vitro cultivation of root organs. The text describes various biological aspects such as the physiology, biochemistry, biodiversity, and life cycles of fungi, as well as the effects of symbiosis on plant growth and development, including large-scale fungus production for biotechnological use. Detailed protocols allow the immediate application of the method to culture mycorrhizal fungi in vitro.