In Vivo And In Vitro Studies With Growing Pigs On Standardised Ileal Amino Acid Digestibilities In Grain Legumes
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In Vivo and in Vitro Studies with Growing Pigs on Standardised Ileal Amino Acid Digestibilities in Grain Legumes by Dagmar Jezierny Pdf
Since there is a general ban on meat and bone meal and its by-products in diets for livestock in the EU, it is crucial to focus on alternative protein feed ingredients to be used in pig nutrition. Beside soybean meal as a commonly used plant protein supplement, grain legumes may also be used as alternative protein sources in diets for pigs. Current protein evaluation system for feed ingredients for pigs are based on the concept of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA), but there is a scarcity of information on SID of CP and AA in grain legumes grown in Central Europe. Therefore, the objective of the thesis was to determine the chemical composition and the SID of CP and AA in seed-grade cultivars of faba beans (Vicia faba), peas (Pisum sativum) and lupins (Lupinus spp.) in growing pigs using the difference method. Furthermore, to obtain SID of CP and AA, the basal ileal endogenous CP and AA losses in growing pigs were estimated by means of regression analysis from apparent ileal digestible and total dietary contents of CP and AA. A further aim of the thesis was directed to the validation of an in vitro procedure for estimates of SID of CP and AA in the same grain legumes by means of a two-step enzymatic method with subsequent pepsin and pancreatin incubations. The in vitro predicted SID values were compared with their corresponding in vivo SID values. The chemical composition and SID values of CP and AA measured in faba bean, pea and lupin cultivars generally were in good agreement with tabulated values. The average SID of CP and most AA in lupin cultivars (CP, 87%) were higher than in pea (CP, 79%) or faba bean cultivars (CP, 76%) Comparably low SID values in some faba bean cultivars can be partly explained by their contents of condensed tannins. In contrast to SID values in faba bean cultivars, there were only small variations in SID values between individual cultivars of peas or lupins. Results of the in vitro experiment revealed that predicted SID of CP and AA do not provide direct estimates of SID values in grain legumes, because the in vitro predicted SID values were generally higher than in vivo SID values. The use of multiple linear regression analysis with grain legume species as a covariate resulted in strong linear relationships. Further investigations are warranted to study if these principles apply to other feed ingredients as well. Moreover, the use of in vitro predicted SID values in practical diet formulations for pigs needs to be further validated.
Potential of cereal grains and grain legumes for modulation of pigs’ intestinal microbiota by Tobias Aumiller Pdf
Native carbohydrate fractions of major dietary components in pig nutrition, such as dietary fiber, resistant starch and oligosaccharides in whole cereal grains or grain legumes appear to be appropriate to manipulate the porcine gut microbiota. Therefore, the objective of this thesis was to evaluate effects of differences in carbohydrate composition of cereal grains, grain legumes and feed supplements on the intestinal microbiota in pigs. Based on a comprehensive literature research, an overview on the current research on the effect of whole grain cereals and grain legumes as well as their by-products on the pigs’ intestinal microbiota in relation to their carbohydrate composition was given. An in vivo experiment was carried out to determine the effects of 8 recently introduced genotypes of barley or wheat on ileal and fecal microbiota of grower pigs by means of quantitative real-time PCR. In addition, in a second experiment the effect of ultra-fine ground fibers from wheat, lupins and peas in comparison to inulin and basal substrates on the microbiota composition and formation of microbial metabolites was determined in vitro using the modified Hohenheim Gas Test. In agreement with previous studies, the potential to modulate the pigs’ intestinal microbiota with cereal grains and feed supplements based on cereal grain or grain legume fibers was shown. However, it was not possible to assign the observed effects on the bacterial groups to the dietary carbohydrates known to influence the intestinal microbiota, such as arabinoxylan or β-glucan.
Recent advances of research in antinutritional factors in legume seeds and oilseeds by M. Muzquiz,G.D. Hill,C. Burbano,C. Cuadrado,M.M. Pedrosa Pdf
"This series of meetings bring together experts working in this field of Science from throughout the world. A major feature of each conference session is an invited review, which outlines the advances that have been made in a particular area since the last meeting. A major factor that was considered at this meeting was the likely impact of plant genetic modification on the nutritional quality of their seeds for human and animal feeding. As an example already a number of legume species and rapeseed have been modified to improve the sulphur amino acid content of their seed and thus their protein quality. Besides the major grain legume species and rapeseed that had been discussed at previous meetings in this series number of crop products, as potential protein sources, for animal feeding, were considered for the first time. These included cottonseed meal, linseed meal, and sunflower seed meal. The potential of some new exotic crops from Mexico was also covered including Mexican species of the genus Lupinus and a Mexican plant from the same family as castor bean, which has a very high oil content but is usually toxic. Work from Cuba compared the nutritional characteristics of soybean with a range of tropical grain legume species, which have received little previous attention. A major change at this meeting was the greater consideration of the effects, both positive, and negative, of the consumption of these seeds for human nutrition. A major review on the development of allergnicity to legume seed in humans is included. There was also consideration of the potential role of antinutritional factors in reducing the growth of various types of tumour cells. The presented papers also suggest that the consumption of legume seed in the diet can potentially reduce serum cholesterol levels. Overall from the 5 conference sessions there are 52 papers. Of these 7 are major invited reviews on the current state of research in this important area for human and animal feeding."
Issues in Animal Science and Research: 2011 Edition by Anonim Pdf
Issues in Animal Science and Research / 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Animal Science and Research. The editors have built Issues in Animal Science and Research: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Animal Science and Research in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Animal Science and Research: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Assessment of standardized ileal amino acid digestibility in different wheat genotypes and wheat concentrated distillers solubles in growing pigs by Pia Rosenfelder Pdf
Wheat is the primarily cereal grain produced within the European Union. Due to its high starch content it is the dominating energy feed ingredient in diets for pigs. However, due to its high dietary inclusion level wheat can also supply considerable high amounts of crude protein (CP) and amino acids (AA) to the animal. Wheat co-products of bioethanol production are a valuable source of CP and AA in animal nutrition. Therefore, the objectives of the present thesis were to study the nutritive value of eight recently introduced wheat genotypes and of one liquid wheat co-product of bioethanol production for growing pigs. For this purpose a comprehensive analysis of nutrients in the assay feedstuffs was conducted and standardized ileal digestibility (SID) of CP and AA in growing pigs was determined. There were significant differences in SID of CP and AA between the eight genotypes of wheat. However, digestibility values were lower when compared to literature data, leading to the assumption that data in actual feed tables might result in an overestimation of the protein value of wheat. The liquid wheat co-product is a valuable source of CP, AA and energy in diets for pigs. It is superior to dried bioethanol co-products as it is not exposed to excessive heat treatment during manufacturing resulting in greater digestibility values. In der Europäischen Union ist Weizen die am weitesten verbreitete Getreideart. Aufgrund seines hohen Stärkegehalts wird Weizen in Mastschweinerationen als Energiefuttermittel eingesetzt. Bei hohen Anteilen in der Gesamtration kann Weizen jedoch auch einen erheblichen Beitrag zur Deckung des Rohprotein- (XP) und Aminosäuren (AS)- Bedarfs des Tieres leisten. Nebenprodukte aus der Weizenverarbeitung bei der Bioethanolproduktion sind eine wertvolle Eiweißkomponente in der Tierernährung, dazu zählen Trocken- und Flüssigschlempen. Ziele dieser Arbeit waren die Bestimmung des Futterwerts von 8 aktuell verfügbaren Weizengenotypen sowie eines flüssigen Weizenschlempefutters für Mastschweine. Zu diesem Zweck wurde eine umfangreiche Nährstoffanalyse der Versuchsfuttermittel durchgeführt, sowie die standardisierte praecaecale Verdaulichkeit (pcVQ) von XP und AS bei Mastschweinen untersucht. Es gab signifikante Unterschiede der pcVQ von XP und AS zwischen den 8 Weizengenotypen. Allerdings waren die Verdaulichkeitswerte geringer als in der Literatur angeben, so dass Daten aus aktuellen Futterwerttabellen möglicherweise den Proteinwert von Weizen überschätzen. Das flüssige Weizenschlempefutter stellt eine wertvolle XP-, AS- und Energiequelle für Schweine dar. Es ist Trockenschlempen im Nährwert überlegen, da es keiner intensiven Hitzeeinwirkung ausgesetzt ist und somit höhere Verdaulichkeitswerte aufweist.
Agriculture, Livestock Production and Aquaculture by Arvind Kumar,Pavan Kumar,S. S. Singh,Bambang Hendro Trisasongko,Meenu Rani Pdf
This two-volume set discusses recent approaches and technological innovations for sustainable agriculture in smallholder farming systems impacted by climate change. The systems covered include crop-based agricultural production, as well as aquaculture and livestock production as related systems using similar techniques to combat food security issues brought about by climate change and resource overuse. The chapters detail innovations involving crop diversification, soil resilience management, geoinformatics and land suitability monitoring for smart farming, information technology in livestock production, and nutrient resource management in fishery aquaculture. Researchers, practitioners and industries will be able to use this information to implement socially and economically sustainable practices to achieve food security in impoverished areas vulnerable to climate change, while also learning about the rapid evolution in information technology that is applicable for and available to small holder farmers. Volume 2 focuses on trends and technologies in food security within the context of sustainable practices, drone technology, microwave data, molecular farming, machine learning, agricultural economics, spatial modeling and agricultural policy. These chapters discuss advancements in fishery resource and aquaculture practices, and also the challenges facing these areas due to climate change.
Standardized ileal amino acid digestibility and basal endogenous losses of amino acids in different genotypes of rye and triticale fed to growing pigs by Elisa Strang Pdf
Cereal grains, such as rye and triticale are a major source of energy in pig nutrition. The crude protein (CP) content in cereal grains is rather low compared to protein ingredients such as soybean meal. However, costs and availability of soybean meal are strongly correlated with the price development of agricultural commodities on the world market. Therefore, swine producers will make increasing efforts to use protein from locally produced cereal grains more efficiently to reduce feed costs. Measurements of apparent (AID) and standardized ileal digestibility (SID) of CP and AA are frequently used to estimate the amounts of absorbable AA from the diet which are used for pigs’ metabolic processes. However, until now there is limited data on SID of CP and AA in rye and triticale, particularly in new genotypes. Therefore, one objective of the present work was to determine the variation in nutritional composition and SID of CP and AA of 8 currently available rye and 8 triticale genotypes. Standardized ileal digestibility of CP and most AA between the 8 assayed genotypes of both rye and triticale did not differ. Furthermore, average SID values of CP and AA of both rye and triticale in the present experiment were lower when compared to values of rye and triticale published in current feed tables. Thus, future research should clarify, whether these differences in comparison to feed tables are caused by experimental conditions or reflect a consistent decrease that need to be accounted for in feed tables.
Assessment of standardized ileal digestibility and basal ileal endogenous losses of amino acids associated with barley in growing pigs by Hanna Spindler Pdf
In 2013, more than 308 million tons of cereals were produced within the European Union, of which about 60 million tons were grown as barley. The protein content of barley is rather low when compared to protein feedstuffs such as soybean meal. However, due to its high dietary inclusion level, up to half of the protein requirement of pigs can be met by barley.
Assessment of Standardised Ileal Crude Protein and Amino Acid Digestibilities in Protein Supplements for Piglets by Renata Urbaityte Pdf
A cautious assessment of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA) as a measure to describe AA bioavailabilities is critical for evaluating the protein value of feed ingredients for piglets. Estimates of SID have been generated for most feed ingredients for grower-finisher pigs, whereas corresponding values for piglets hardly exist. Until now, SID values determined in grower-finisher pigs are used in diet formulation for piglets as well. However, there is some concern that these values may not be valid in piglets due to a limited digestive capacity for feed protein in piglets. A guideline for the standardisation of the experimental procedure in digestibility experiments had already been proposed for grower-finisher pigs. These guidelines firstly include threshold levels for CP and AA in the assay diets to obtain so-called plateau apparent ileal digestibility values, which are independent of dietary CP and AA levels, and secondly values for IAALB. For piglets, similar guidelines, including dietary threshold levels and values for IAALB, have currently been established. Therefore, the objective of the thesis was, based on these guidelines, to determine SID of CP and AA in 24 assay feed ingredients, including 11 products from soybean processing, 7 by-products from starch processing, 2 fish meals and 4 whey proteins. For this purpose, a total of 4 digestibility experiments with piglets fitted with simple T-cannulas at the distal ileum was conducted. For each experiment, 14 barrows were weaned at 18 days of age. Each experiment consisted of 3 periods for digesta collection. A total of 24 semisynthetic cornstarch-based diets with various inclusion levels of casein were formulated to which 1 of the 24 assay feed ingredients each was added. The daily feed allowance was restricted to 30 g/kg of individual body weight throughout all experimental periods. The SID values were determined by difference to SID values in casein. The results of the present studies show large variations in SID values among differently processed soybean products (p0.05). The SID values in extruded soybeans (SBe) were lower compared to high-protein soybean meal (SBMhp), soy protein concentrate (SPC) or partially hydrolysed soy protein isolate (SPIh) (p0.05). Aqueous alcohol or water extraction in SPC or SPIh improved SID values compared to SBMhp (p0.05). The SID values among the 4 batches of SPC were similar (p0.05) and consistently high. Moreover, the SID values were similar in SPC and SPIh (p0.05). The SID values of in alternatively processed soy proteins including enzymatically fermented SPA and microbially fermented SPB, were inconsistent. For the 3 SPA products, SID increased numerically from values similar to SBMhp to values similar to SPC with increasing duration of enzymatic treatment. For SPB SID values were low and similar to SBe, which may be attributed to protein denaturation during microbial fermentation. The results of present study show for most AA similar SID values for all by-products of starch processing including pea protein (PeaP), wheat gluten (WG), corn gluten (CG), and potato protein (PotP) compared to SPC (p0.05) except for SID of Lys in WG and CG, and SID of Trp in CG were considerably lower compared to other products (p0.05). The particular low SID of Lys and Trp in WG and CG might be simply a reflection of an experimental error, because SID values by means of the difference method rely on sufficient high AA contribution levels to the assay diets, which was not achieved for the aforementioned AA. The results of the present study show that SID values in fish meal (FM1) and extracted fish meal (FM2e) vary considerably (p
Author : M. F. Fuller Publisher : Oxford University Press, USA Page : 230 pages File Size : 42,5 Mb Release : 1991 Category : Medical ISBN : WISC:89037851573
In Vitro Digestion for Pigs and Poultry by M. F. Fuller Pdf
What are we trying to simulate?; Digestive enzyme activities in pigs and poultry; The role of the microflora in digestion; The physical and chemical constitution of foods: effects on carbohydrate digestion; Indirect effects of foods antinutrients on protein digestibility and nutritional value of diets; In vitro simulation of digestion and absorptionin the gastro-intestinal tract of pigs and poultry - methods and results; The chemical and biological bases of a calculation system developed for predicting dietary energy values: a poultry model; Enzyme modelling of protein digestion and L-lysine availability; Use of pepsin digentibility, multienzyme pH change and protein solubility assays to predict in vivo protein quality of feedstuffs; Estimation of true ileal digestibility of amino acids with pigs by an in vitro method using intestinal fluid; prediction of the energy value of non-ruminant feeds using in vitro digestion with intestinal fluid and other chemical methods; A model for feed evaluation based on in vitro digestible dry matter and protein; In vitro simulation of protein digestion: an integrated approach; Simultaneous estimations of precaecal protein and carbohydrate digestion in the pig; The way forward; the future requirements of the feed industry; The scientific challenge.
Nutrient and energy digestibility in weaned piglets as influenced by feeding level and origin of soy-bean meal in the diet by Miriam Goerke Pdf
Feed is the greatest single cost factor in pig production, and protein and energy accounts for the largest proportion of feed costs. Therefore, it is crucial that the protein and energy content of a diet is characterized in the best possible way. Over the past years, the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) has been introduced into diet formulation for pigs in several countries. Moreover, different energy systems are available for feed evaluation including digestible energy (DE), metabolizable energy (ME) and net energy (NE). For soybean meal (SBM), the most commonly used feed ingredient in pig diets, tabulated values for SID of AA in addition to DE, ME or NE contents have been determined in grower-finisher pigs rather than weaned piglets. Due to the lack of separate feed tables for piglets, tabulated values originating from experiments with grower-finisher pigs are being used for diet formulation in piglet feeding, although post-weaning feed intake (FI) is highly variable and often lower than 3 times the maintenance energy requirement of weaned piglets. Thus, it remains open, if values obtained with grower-finisher pigs can be used for piglets as well. Furthermore, in grower-finisher pigs it has been shown that dietary inclusion of SBM sources of different origins may affect SID of CP and AA. The influence of SBM origin on SID of CP and AA in piglets has not been investigated so far. Therefore, the first objective of the present thesis was to determine SID of CP and AA in a large variety of SBM sources sourced from different countries, whereas the second and third objective was to investigate the effect of FI level on apparent ileal digestibility (AID) and SID of CP and AA, as well as on apparent total tract digestibility (ATTD) of proximate nutrients and energy in weaned piglets. For this purpose, a digestibility experiment with a total of 36 (initial BW 5.6 kg) newly weaned pigs, surgically fitted with simple T-cannulas at the distal ileum, was conducted. The piglets were housed in metabolic crates, and were fed semi-synthetic diets based on cornstarch and 1 out of 18 different SBM batches originating either from Argentina (6 batches), Brazil (6 batches) or the United States (US; 6 batches). Diets were fed on three graded levels of FI corresponding to 30, 45 and 60 g per kg average body weight (BW) of piglets, equivalent to 50, 75 or 100 g/kg BW0.75 d or 1.9, 2.6 and 3.1 times the energy requirement for maintenance. The whole experiment was split in 3 consecutive parts with 12 piglets, each. Over the whole experiment, the piglets were randomly allocated to the different combinations of FI level and SBM, but balanced in order to achieve 3 identical experimental parts. Chapter 1 of the present thesis provides a brief introduction into the objective of this thesis, including background information on SBM production, composition, and a literature review about studies concerning the impact of SBM origin and the effect of FI level on nutrient digestibility in pigs. Chapter 2 includes information on the chemical composition and proximate constituents of the 18 SBM batches originating from Argentina, Brazil, or the US. The average content of CP was greater for Brazilian SBM (P ≤ 0.05) compared to the other 2 origins. Contents of most AA were greater (P ≤ 0.05) in Brazilian compared to Argentinean SBM batches. Amino acid contents in US SBM batches ranged between those from Argentina and Brazil. Average trypsin inhibitor activity was greatest (P ≤ 0.05) in Brazilian SBM. Mycotoxins were detected in 8 out of 18 SBM batches, but all mycotoxin levels were below their critical benchmarks. The contents of individual isoflavones varied considerably among SBM origins (P ≤ 0.05). The SID of Arg, Phe, Thr, Trp, Asp, Gly, and Ser were greater (P ≤ 0.05) for US compared to Argentinean SBM batches, and intermediate values were obtained for Brazilian SBM batches. The SID values were most variable within Argentinean SBM batches and most homogenous within US SBM batches, as indicated by a great and a small coefficient of variation, respectively. However, SID of CP and AA were not related to any of the analyzed chemical constituents. It was concluded that EU imports of SBM are generally of high quality. The obtained SID values contribute substantially to the expansion of databases on SID of CP and AA in SBM from the 3 major soybean-producing countries for piglets. However, SID values obtained with piglets were considerably lower compared with tabulated values originating from grower-finisher pigs. Thus, further studies are warranted to confirm that finally separate feed tables for ingredients used in diet formulation for piglets are required to account for these differences. In Chapter 3, the effect of 3 graded levels of FI on AID and SID of CP and AA in SBM based diets was determined in piglets. The AID and SID of most AA were quadratically affected by the FI level (P ≤ 0.05). Initially, both AID and SID of most AA increased up to 1.9 % units as the FI level was increased from 30 to 45 g/kg BW. Thereafter, these AID and SID values decreased by 2.6 and 2.7 % units, respectively, as the FI level was further increased from 45 to 60 g/kg BW. It was concluded that there is a significant effect of feed intake level, albeit of small magnitude, on AID and SID of CP and AA, which possibly has to be accounted for in feed tables, due to the high variability of voluntary FI post weaning. In Chapter 4, the effect of 3 graded FI levels on AID and ATTD of proximate nutrients and energy, as well as on estimates of dietary DE, ME and NE contents was investigated. Due to the graded level of FI, and thereby the graded intake of nutrients, ileal endogenous nutrient losses and/or total tract endogenous nutrient losses could be estimated by linear regression analysis, and true digestibility values as well as urinary endogenous N losses could be calculated. The AID of DM, N and energy as well as ATTD of neutral detergent fiber, acid detergent fiber and ether extract (EE) in the assay diets was not affected (P > 0.05) by FI level. However, there was a small decrease in ATTD of DM, N, OM, ash and energy, as well as in DE, ME and NE content in the assay diets (P
Nutritional and Physiological Functions of Amino Acids in Pigs by Francois Blachier,Guoyao Wu,Yulong Yin Pdf
This book provides developmental data regarding piglets (with a focus on the gastrointestinal tract), data related to amino acid metabolism in pigs, data related to nutritional and physiological functions of amino acids in pigs, nutritional requirements for amino acids in pigs, signaling roles of amino acids, methodological aspects in amino acid research and the pig model for studying amino acid-related human diseases.
Evaluation of amino acid digestibility values of protein feedstuffs and dietary protein content in combination with probiotic supplementation in growing pigs by Chanwit Kaewtapee Pdf
During the last decade, the concept of standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) has been used in diet formulation for pigs. However, there is limited data on the nutritional composition and SID of CP and AA in home-grown European soybean and rapeseed products. For full-fat soybeans (FFSB), the presence of heat-labile trypsin inhibitors may cause problems in protein digestion of pigs. Therefore, the optimization of heat treatment for FFSB is a need to reduce trypsin inhibitor activity for improving SID of CP and AA. However, excessive heat treatment may have a negative effect on protein digestion. Furthermore, reducing dietary protein levels in combination with the supplementation of Bacillus spp. may reduce feed costs and improve pigs’ gut health. As a result of the present study, home-grown European soybean and rapeseed products can be considered as a suitable alternative to imported soybean products for pigs raised in organic and conventional farming systems. Wet heating at 100°C for 16 min, together with autoclaving at 110°C from 15 to 45 min reflects optimal heat treatment for FFSB resulting in greater SID of CP and AA with lower destruction of AA. Reducing the dietary CP level is an alternative strategy to improve SID of some AA, thereby decreasing feed cost and nitrogen excretion. The supplementation of Bacillus spp. promoted gene copy numbers of Roseburia spp., which may be beneficial due to ascribed health promoting properties of this butyrate producer.
Swine Nutrition by Austin J. Lewis,L. Lee Southern Pdf
With 42 chapters authored by leading international experts, Swine Nutrition: Second Edition is a comprehensive reference that covers all aspects of the nutrition of pigs. Content includes characteristics of swine and the swine industry with emphasis on the gastrointestinal tract; various classes of nutrients, how these nutrients are metabolized by swine, and the factors affecting their utilization; the practical aspects of swine nutrition from birth through gestation, lactation in sows, and the feeding of adult boars; and nutritional aspects of the various feedstuffs commonly fed to swine. Rounding the book is coverage of various techniques used in swine nutrition research.