Initial Rate Enzyme Kinetics

Author : H. J. Fromm
Publisher : Springer Science & Business Media
Page : 332 pages
File Size : 55,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783642809668

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Initial Rate Enzyme Kinetics by H. J. Fromm Pdf

Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.

Author : Athel Cornish-Bowden
Publisher : Elsevier
Page : 221 pages
File Size : 40,5 Mb
Release : 2014-05-20
Category : Science
ISBN : 9781483164670

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Principles of Enzyme Kinetics by Athel Cornish-Bowden Pdf

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Author : Vladimir Leskovac
Publisher : Springer Science & Business Media
Page : 438 pages
File Size : 55,9 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306483905

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Comprehensive Enzyme Kinetics by Vladimir Leskovac Pdf

Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
Page : 237 pages
File Size : 47,6 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306480256

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Enzyme Kinetics and Mechanisms by Kenneth B. Taylor Pdf

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.

Author : Athel Cornish-Bowden
Publisher : Elsevier
Page : 245 pages
File Size : 47,5 Mb
Release : 2014-05-20
Category : Science
ISBN : 9781483161198

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Fundamentals of Enzyme Kinetics by Athel Cornish-Bowden Pdf

Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.

Author : Daniel L. Purich
Publisher : Elsevier
Page : 920 pages
File Size : 51,7 Mb
Release : 2010-06-16
Category : Science
ISBN : 9780123809254

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Enzyme Kinetics: Catalysis and Control by Daniel L. Purich Pdf

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Author : Paul F. Cook,W. W. Cleland
Publisher : Garland Science
Page : 416 pages
File Size : 48,7 Mb
Release : 2007-03-06
Category : Medical
ISBN : 9781136844287

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Enzyme Kinetics and Mechanism by Paul F. Cook,W. W. Cleland Pdf

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Author : Kent M. Plowman
Publisher : McGraw-Hill Companies
Page : 198 pages
File Size : 51,7 Mb
Release : 1971
Category : Science
ISBN : MINN:31951000464538K

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Enzyme Kinetics by Kent M. Plowman Pdf

Author : Raymond Chang
Publisher : University Science Books
Page : 706 pages
File Size : 53,8 Mb
Release : 2005-02-11
Category : Science
ISBN : 1891389335

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Physical Chemistry for the Biosciences by Raymond Chang Pdf

This book is ideal for use in a one-semester introductory course in physical chemistry for students of life sciences. The author's aim is to emphasize the understanding of physical concepts rather than focus on precise mathematical development or on actual experimental details. Subsequently, only basic skills of differential and integral calculus are required for understanding the equations. The end-of-chapter problems have both physiochemical and biological applications.

Author : Daniel L. Purich
Publisher : Academic Press
Page : 567 pages
File Size : 48,8 Mb
Release : 1983-01-01
Category : Science
ISBN : 9781483214221

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Contemporary Enzyme Kinetics and Mechanism by Daniel L. Purich Pdf

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Author : Stanley Ainsworth
Publisher : Unknown
Page : 255 pages
File Size : 55,6 Mb
Release : 1977
Category : Chemical kinetics
ISBN : 0333150082

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Steady-state Enzyme Kinetics by Stanley Ainsworth Pdf

Author : Alejandro G. Marangoni
Publisher : John Wiley & Sons
Page : 247 pages
File Size : 46,6 Mb
Release : 2003-04-23
Category : Science
ISBN : 9780471461418

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Enzyme Kinetics by Alejandro G. Marangoni Pdf

Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.

Author : Anonim
Publisher : Academic Press
Page : 703 pages
File Size : 45,6 Mb
Release : 2009-10-24
Category : Science
ISBN : 9780123847447

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Contemporary Enzyme Kinetics and Mechanism by Anonim Pdf

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Author : Daniel L. Purich,R. Donald Allison
Publisher : Elsevier
Page : 788 pages
File Size : 54,6 Mb
Release : 1999-10-26
Category : Science
ISBN : 0080521932

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Handbook of Biochemical Kinetics by Daniel L. Purich,R. Donald Allison Pdf

Biochemical kinetics refers to the rate at which a reaction takes place. Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material. The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on techniques and their likely limitations and pitfalls, as well as derivations of fundamental rate equations that characterize biochemical processes. Key Features * Over 750 pages devoted to theory and techniques for studying enzymic and metabolic processes * Over 1,500 definitions of kinetic and mechanistic terminology, with key references * Practical advice on experimental design of kinetic experiments * Extended step-by-step methods for deriving rate equations * Over 1,000 enzymes, complete with EC numbers, reactions catalyzed, and references to reviews and/or assay methods * Over 5,000 selected references to kinetic methods appearing in the Methods in Enzymology series * 72-page Wordfinder that allows the reader to search by keywords * Summaries of mechanistic studies on key enzymes and protein systems * Over 250 diagrams, figures, tables, and structures

Author : Anonim
Publisher : Unknown
Page : 0 pages
File Size : 46,9 Mb
Release : 1979
Category : Electronic
ISBN : OCLC:910066243

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Enzyme Kinetics and Mechanism by Anonim Pdf