Contemporary Enzyme Kinetics And Mechanism

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Contemporary Enzyme Kinetics and Mechanism

Author : Daniel L. Purich
Publisher : Academic Press
Page : 567 pages
File Size : 42,7 Mb
Release : 1983-01-01
Category : Science
ISBN : 9781483214221

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Contemporary Enzyme Kinetics and Mechanism by Daniel L. Purich Pdf

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Contemporary Enzyme Kinetics and Mechanism

Author : Anonim
Publisher : Academic Press
Page : 703 pages
File Size : 43,7 Mb
Release : 2009-10-24
Category : Science
ISBN : 9780123847447

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Contemporary Enzyme Kinetics and Mechanism by Anonim Pdf

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Enzyme Kinetics and Mechanism

Author : Paul F. Cook,W. W. Cleland
Publisher : Garland Science
Page : 416 pages
File Size : 45,7 Mb
Release : 2007-03-06
Category : Medical
ISBN : 9781136844287

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Enzyme Kinetics and Mechanism by Paul F. Cook,W. W. Cleland Pdf

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Enzyme Kinetics: Catalysis and Control

Author : Daniel L. Purich
Publisher : Elsevier
Page : 920 pages
File Size : 55,5 Mb
Release : 2010-06-16
Category : Science
ISBN : 9780123809254

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Enzyme Kinetics: Catalysis and Control by Daniel L. Purich Pdf

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Enzyme Kinetics and Mechanisms

Author : Kenneth B. Taylor
Publisher : Springer Science & Business Media
Page : 237 pages
File Size : 49,6 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306480256

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Enzyme Kinetics and Mechanisms by Kenneth B. Taylor Pdf

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature.

Comprehensive Enzyme Kinetics

Author : Vladimir Leskovac
Publisher : Springer Science & Business Media
Page : 438 pages
File Size : 51,7 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306483905

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Comprehensive Enzyme Kinetics by Vladimir Leskovac Pdf

Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

Essential Enzyme Kinetics

Author : Daniel L. Purich
Publisher : Academic Press
Page : 425 pages
File Size : 43,6 Mb
Release : 2020-06
Category : Science
ISBN : 0128218088

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Essential Enzyme Kinetics by Daniel L. Purich Pdf

Essentials of Enzyme Kinetics: A Textbook for Molecular Life Scientists discusses best-practice approaches for using site-directed mutagenesis to explore enzyme catalysis. The book presents a balance of initial-rate methods, fast reaction techniques, and kinetic isotope effect approaches, providing a must-have reference for bioscience workers and investigators who seek a better understanding of enzyme kinetics. Non-biochemists entering careers in Big Pharma will also benefit from the book's content as covered topics have become main drivers in small-molecule drug development. Offers practical information on how to work with enzymes and design experiments to identify new inhibitors or activators Provides detailed, step-by-step derivations of rate equations, showing tried-and-true ways to confirm that equations are correct Includes 10-12 problems for each chapter

Initial Rate Enzyme Kinetics

Author : H. J. Fromm
Publisher : Springer Science & Business Media
Page : 332 pages
File Size : 42,9 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783642809668

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Initial Rate Enzyme Kinetics by H. J. Fromm Pdf

Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.

Principles of Enzyme Kinetics

Author : Athel Cornish-Bowden
Publisher : Elsevier
Page : 221 pages
File Size : 41,9 Mb
Release : 2014-05-20
Category : Science
ISBN : 9781483164670

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Principles of Enzyme Kinetics by Athel Cornish-Bowden Pdf

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Enzyme Kinetics and Mechanism

Author : Daniel L. Purich
Publisher : Unknown
Page : 0 pages
File Size : 48,6 Mb
Release : 1979
Category : Chemical kinetics
ISBN : LCCN:79018746

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Enzyme Kinetics and Mechanism by Daniel L. Purich Pdf

Enzyme Kinetics

Author : Paul C. Engel
Publisher : Springer
Page : 97 pages
File Size : 41,7 Mb
Release : 2013-11-21
Category : Science
ISBN : 9781489932785

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Enzyme Kinetics by Paul C. Engel Pdf

The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.

Enzyme Kinetics and Mechanism, Part D: Developments in Enzyme Dynamics

Author : Anonim
Publisher : Academic Press
Page : 0 pages
File Size : 55,7 Mb
Release : 1995-03-22
Category : Science
ISBN : 0121821501

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Enzyme Kinetics and Mechanism, Part D: Developments in Enzyme Dynamics by Anonim Pdf

General Description of the Volume: This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Enzymes

Author : Robert A. Copeland
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 41,5 Mb
Release : 2004-04-07
Category : Science
ISBN : 9780471461852

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Enzymes by Robert A. Copeland Pdf

Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research.

Enzyme Kinetics and Mechanism Part D

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 44,7 Mb
Release : 1995
Category : Electronic
ISBN : OCLC:760664062

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Enzyme Kinetics and Mechanism Part D by Anonim Pdf

Enzymes

Author : Robert Allen Copeland
Publisher : Wiley-VCH
Page : 336 pages
File Size : 49,8 Mb
Release : 1996
Category : Medical
ISBN : UOM:39015037847798

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Enzymes by Robert Allen Copeland Pdf

Supplemented with appendices providing contact information for suppliers of reagents and equipment for enzyme studies, as well as a survey of useful Internet sites and computer software for enzymatic data analysis, Enzymes, Second Edition is the ultimate practical guide for scientists and students in biochemical, pharmaceutical, biotechnical, medicinal, and agricultural/food-related research.