Enzyme Kinetics

Author : Athel Cornish-Bowden
Publisher : Elsevier
Page : 244 pages
File Size : 45,6 Mb
Release : 2014-05-20
Category : Science
ISBN : 9781483161198

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Fundamentals of Enzyme Kinetics by Athel Cornish-Bowden Pdf

Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.

Author : Irwin H. Segel
Publisher : Unknown
Page : 957 pages
File Size : 48,5 Mb
Release : 2014
Category : Enzyme kinetics
ISBN : 8126548150

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Enzyme Kinetics by Irwin H. Segel Pdf

Author : Hans Bisswanger
Publisher : John Wiley & Sons
Page : 320 pages
File Size : 51,7 Mb
Release : 2017-06-22
Category : Science
ISBN : 9783527806478

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Enzyme Kinetics by Hans Bisswanger Pdf

Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics. Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application. With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work.

Author : Daniel L. Purich
Publisher : Elsevier
Page : 920 pages
File Size : 45,9 Mb
Release : 2010-06-16
Category : Science
ISBN : 9780123809254

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Enzyme Kinetics: Catalysis and Control by Daniel L. Purich Pdf

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Author : Robert A. Alberty
Publisher : John Wiley & Sons
Page : 448 pages
File Size : 44,9 Mb
Release : 2011-03-10
Category : Science
ISBN : 9781118002155

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Enzyme Kinetics by Robert A. Alberty Pdf

Rapid-Equilibrium Enzyme Kinetics helps readers emphasize the estimation of kinetic parameters with the minimum number of velocity measurements, thereby reducing the amount of laboratory work necessary, and allowing more time for the consideration of complicated mechanisms. The book systematically progresses through six levels of understanding the enzyme-catalyzed reaction, and includes a CD-ROM so that the reader may use the programs in the book to input their own experimental data.

Author : Athel Cornish-Bowden
Publisher : Elsevier
Page : 220 pages
File Size : 48,8 Mb
Release : 2014-05-20
Category : Science
ISBN : 9781483164670

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Principles of Enzyme Kinetics by Athel Cornish-Bowden Pdf

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Author : N.S. Punekar
Publisher : Springer
Page : 562 pages
File Size : 51,8 Mb
Release : 2018-11-11
Category : Science
ISBN : 9789811307850

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ENZYMES: Catalysis, Kinetics and Mechanisms by N.S. Punekar Pdf

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Author : Daniel L. Purich
Publisher : Academic Press
Page : 566 pages
File Size : 40,7 Mb
Release : 1983-01-01
Category : Science
ISBN : 9781483214221

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Contemporary Enzyme Kinetics and Mechanism by Daniel L. Purich Pdf

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Author : Vladimir Leskovac
Publisher : Springer Science & Business Media
Page : 438 pages
File Size : 45,9 Mb
Release : 2007-05-08
Category : Science
ISBN : 9780306483905

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Comprehensive Enzyme Kinetics by Vladimir Leskovac Pdf

Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

Author : Alejandro G. Marangoni
Publisher : John Wiley & Sons
Page : 247 pages
File Size : 49,5 Mb
Release : 2003-04-23
Category : Science
ISBN : 9780471461418

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Enzyme Kinetics by Alejandro G. Marangoni Pdf

Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.

Author : Arthur R. Schulz
Publisher : Cambridge University Press
Page : 264 pages
File Size : 42,7 Mb
Release : 1994-11-25
Category : Science
ISBN : 0521449502

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Enzyme Kinetics by Arthur R. Schulz Pdf

This text covers the field of steady-state kinetics from basic principles to the control of the multi-enzyme systems which constitute metabolic pathways. Emphasis is placed on the interpretation of the kinetic behaviour of enzyme-catalyzed reactions in terms of mechanisms. Algorithms are developed which can be implemented in computer programs for the derivation of equations. The treatment of steady-state enzyme kinetics is extended to allosteric enzymes and subunit interactions in polymeric enzymes. Principles are presented which provide for mathematical analysis of the control of multi-enzyme systems. Problems are included at the end of each chapter and their solutions are found at the end of the book. This book will be a useful text for advanced undergraduates and graduate students taking courses in enzyme chemistry and enzyme kinetics.

Author : H. J. Fromm
Publisher : Springer Science & Business Media
Page : 332 pages
File Size : 45,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783642809668

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Initial Rate Enzyme Kinetics by H. J. Fromm Pdf

Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.

Author : Herbert M. Sauro
Publisher : Future Skill Software
Page : 320 pages
File Size : 45,8 Mb
Release : 2011
Category : Science
ISBN : 9780982477311

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Enzyme Kinetics for Systems Biology by Herbert M. Sauro Pdf

This book introduces fundamental concepts in kinetics that relate to system biology. Topics include basic kinetics, enzyme kinetics, generalized rate laws, gene regulation and introduction to elasticities. The text is suitable for junior/senior undergraduates and graduates who need access to information relevant to modeling biochemical pathways.

Author : Anonim
Publisher : Academic Press
Page : 703 pages
File Size : 42,5 Mb
Release : 2009-10-24
Category : Science
ISBN : 9780123847447

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Contemporary Enzyme Kinetics and Mechanism by Anonim Pdf

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Author : Paul F. Cook,W. W. Cleland
Publisher : Garland Science
Page : 416 pages
File Size : 49,6 Mb
Release : 2007-03-06
Category : Medical
ISBN : 9781136844287

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Enzyme Kinetics and Mechanism by Paul F. Cook,W. W. Cleland Pdf

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.