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Introduction to Fluorescence

David M. Jameson

Author : David M. Jameson
Publisher : Taylor & Francis
Page : 313 pages
File Size : 40,6 Mb
Release : 2014-01-22
Category : Science
ISBN : 9781439806050

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Introduction to Fluorescence by David M. Jameson Pdf

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.

Introduction to Fluorescence Sensing

Alexander P. Demchenko

Author : Alexander P. Demchenko
Publisher : Springer
Page : 0 pages
File Size : 45,6 Mb
Release : 2019-03-13
Category : Medical
ISBN : 3319793187

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Introduction to Fluorescence Sensing by Alexander P. Demchenko Pdf

Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.

Principles of Fluorescence Spectroscopy

Joseph R. Lakowicz

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 41,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461576587

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

An Introduction to Fluorescence Correlation Spectroscopy

Thorsten Wohland,Sudipta Maiti,Radek Machan

Author : Thorsten Wohland,Sudipta Maiti,Radek Machan
Publisher : Iop Expanding Physics
Page : 300 pages
File Size : 45,6 Mb
Release : 2020-11-04
Category : Science
ISBN : 0750320788

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An Introduction to Fluorescence Correlation Spectroscopy by Thorsten Wohland,Sudipta Maiti,Radek Machan Pdf

An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Key Features Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook. Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes. Provides rigorous mathematical derivations of fundamental FCS equations and models. Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS.

Introduction to Confocal Fluorescence Microscopy

Michiel Muller

Author : Michiel Muller
Publisher : SPIE Press
Page : 142 pages
File Size : 48,5 Mb
Release : 2006
Category : Science
ISBN : 0819460435

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Introduction to Confocal Fluorescence Microscopy by Michiel Muller Pdf

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Introduction to Fluorescence Spectroscopy

Ashutosh Sharma,Stephen G. Schulman

Author : Ashutosh Sharma,Stephen G. Schulman
Publisher : Wiley-Interscience
Page : 200 pages
File Size : 44,6 Mb
Release : 1999-05-21
Category : Science
ISBN : UOM:39015050036279

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Introduction to Fluorescence Spectroscopy by Ashutosh Sharma,Stephen G. Schulman Pdf

An accessible guide to all aspects of molecular fluorescence spectroscopy This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. Written by two highly respected experts in the field, Introduction to Fluorescence Spectroscopy covers all aspects of the technology-physical fundamentals, instrumentation, methods, and applications. The information is offered at 0a very practical level and addresses a broad range of chemical, physical, biological, and geological problems. The authors incorporate recent advances in commercially available instrumentation as well as fluorescent derivatizing agents, provide many examples of state-of-the-art applications, and discuss future trends. Concise, accessible, up-to-date, Introduction to Fluorescence Spectroscopy is an indispensable reference and an invaluable primer for those involved in the field of analytical science and other professionals interested in this fast-evolving analytical technique.

Molecular Fluorescence

Bernard Valeur,Mário Nuno Berberan-Santos

Author : Bernard Valeur,Mário Nuno Berberan-Santos
Publisher : John Wiley & Sons
Page : 595 pages
File Size : 43,5 Mb
Release : 2013-03-25
Category : Science
ISBN : 9783527328468

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Molecular Fluorescence by Bernard Valeur,Mário Nuno Berberan-Santos Pdf

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Introduction to Fluorescence In Situ Hybridization

Michael Andreeff,Daniel Pinkel

Author : Michael Andreeff,Daniel Pinkel
Publisher : Wiley-Liss
Page : 0 pages
File Size : 55,5 Mb
Release : 1999-12-03
Category : Medical
ISBN : 0471013455

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Introduction to Fluorescence In Situ Hybridization by Michael Andreeff,Daniel Pinkel Pdf

Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D., and Daniel Pinkel, PH.D. Fluorescence In Situ Hybridization (FISH) has become an essential tool in the diagnosis and management of a variety of solid tumors and hematologic malignancies in the clinical setting, as well as an aid in the identification of particular genetic disorders. Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH and covers in detail the applications of this technology to cancer in humans, including tumorigenesis, prostate and breast tumors, myeloid leukemias, and lymphoproliferative malignancies. The final section of the book is devoted to the applications of FISH techniques to genetic analysis. This incorporates radiation biodosimetry in chromosomal aberrations, genetic toxicology, and multiprobe methods for detecting chromosomally defective sperm in humans and mice, plus rapid prenatal diagnosis of chromosomal aneuploidies and detection of fetal trisomy. Edited by two leading basic and clinical investigators in the field, with state-of-the-art contributions from expert subspecialists, and featuring over 200 color illustrations, Introduction to Fluorescence In Situ Hybridization is an essential reference for clinicians and investigators in oncology, genetics, and cytometry.

Aquatic Organic Matter Fluorescence

Paula G. Coble,Jamie Lead,Andy Baker,Darren M. Reynolds,Robert G. M. Spencer

Author : Paula G. Coble,Jamie Lead,Andy Baker,Darren M. Reynolds,Robert G. M. Spencer
Publisher : Cambridge University Press
Page : 407 pages
File Size : 44,7 Mb
Release : 2014-07-14
Category : Science
ISBN : 9780521764612

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Aquatic Organic Matter Fluorescence by Paula G. Coble,Jamie Lead,Andy Baker,Darren M. Reynolds,Robert G. M. Spencer Pdf

A core text on principles, laboratory/field methodologies, and data interpretation for fluorescence applications in aquatic science, for advanced students and researchers.

Fluorescence Lifetime Spectroscopy and Imaging

Laura Marcu,Paul M. W. French,Daniel S. Elson

Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
Publisher : CRC Press
Page : 554 pages
File Size : 55,6 Mb
Release : 2014-07-17
Category : Science
ISBN : 9781439861684

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Fluorescence Lifetime Spectroscopy and Imaging by Laura Marcu,Paul M. W. French,Daniel S. Elson Pdf

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Introduction to Fluorescence Microscopy

J. S. Ploem,H. J. Tanke

Author : J. S. Ploem,H. J. Tanke
Publisher : Garland Science
Page : 72 pages
File Size : 55,6 Mb
Release : 1987
Category : Language Arts & Disciplines
ISBN : UOM:39015012610625

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Introduction to Fluorescence Microscopy by J. S. Ploem,H. J. Tanke Pdf

Covers three main areas.The phenomenon of fluorescence, the main applications, and the most common problems.

New Trends in Fluorescence Spectroscopy

Bernard Valeur,Jean-Claude Brochon

Author : Bernard Valeur,Jean-Claude Brochon
Publisher : Springer Science & Business Media
Page : 496 pages
File Size : 55,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642568534

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New Trends in Fluorescence Spectroscopy by Bernard Valeur,Jean-Claude Brochon Pdf

This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Fluorescence Microscopy

Ulrich Kubitscheck

Author : Ulrich Kubitscheck
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 42,6 Mb
Release : 2017-03-27
Category : Medical
ISBN : 9783527687725

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Fluorescence Microscopy by Ulrich Kubitscheck Pdf

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy in Life Sciences

Juan Carlos Stockert ,Alfonso Blazquez-Castro

Author : Juan Carlos Stockert ,Alfonso Blazquez-Castro
Publisher : Bentham Science Publishers
Page : 751 pages
File Size : 54,9 Mb
Release : 2017-12-15
Category : Science
ISBN : 9781681085180

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Fluorescence Microscopy in Life Sciences by Juan Carlos Stockert ,Alfonso Blazquez-Castro Pdf

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.