Laboratory Manual For Genetic Engineering

Author : VENNISON, S. JOHN
Publisher : PHI Learning Pvt. Ltd.
Page : 140 pages
File Size : 40,5 Mb
Release : 2009-01-01
Category : Science
ISBN : 9788120338142

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Laboratory Manual For Genetic Engineering by VENNISON, S. JOHN Pdf

This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. KEY FEATURES : Includes about 60 different experiments. Contains several figures to reinforce the understanding of the techniques discussed. Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.

Author : Hala M. Abdelmigid,Hala M Abdelmigid, Dr
Publisher : CreateSpace
Page : 112 pages
File Size : 49,5 Mb
Release : 2013-11
Category : Science
ISBN : 1492832553

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Genetic Engineering by Hala M. Abdelmigid,Hala M Abdelmigid, Dr Pdf

The primary motive for compiling and publishing this manual was to provide scientists, researchers, and students from national agricultural research systems, universities, and small private companies in developing countries, as well as advanced research institutions in the developed world, with a useful guide on the protocols currently in use in genetic engineering. This manual is intended to introduce you to some of the most widely used experimental procedures in biotechnology, including DNA isolation, manipulation, and cloning. You will also gain some familiarity with some of the types of equipment frequently used in biochemistry and molecular biology.The objective of this laboratory course is to provide you with hands-on experience in some of the basic, but essential laboratory skills required in molecular biology and biotechnology. Emphasis will be placed on understanding the concepts behind designing and implementing controlled experiments. The genetic engineering laboratory, like all laboratory courses, is an exploration of procedures. This means that, in order to get full benefit from the course, you will need to read the Manual; this reading will provide background information and an outline of the procedures to be performed. If you do not do this, you will find yourself wasting large amounts of class time, and annoying both your lab partners and your instructor. To encourage your understanding of the material, you will have problem sets that cover material related to the planned experiments. The genetic engineering laboratory is conducted as a “directed” research project. This means that although the general procedures are well established, the overall goal of each experiment is the acquisition of new information. Because of the nature of scientific research, predicting the outcome of experiments that have not previously been performed is difficult. It may therefore be necessary to design new experiments based on the results of previous ones, or to repeat experiments that yielded ambiguous results. On the other hand, if you approach the course will an open and flexible mindset, you will learn how research is performed in a genetic engineering laboratory.

Author : Melody S. Clark
Publisher : Springer Science & Business Media
Page : 551 pages
File Size : 53,5 Mb
Release : 2013-11-27
Category : Science
ISBN : 9783642878732

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Plant Molecular Biology — A Laboratory Manual by Melody S. Clark Pdf

Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.

Author : Sue Carson,Dominique Robertson
Publisher : Elsevier
Page : 172 pages
File Size : 53,7 Mb
Release : 2005-12-15
Category : Science
ISBN : 9780080456546

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Manipulation and Expression of Recombinant DNA by Sue Carson,Dominique Robertson Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Isil Aksan Kurnaz
Publisher : CRC Press
Page : 346 pages
File Size : 52,6 Mb
Release : 2015-05-08
Category : Medical
ISBN : 9781482260908

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Techniques in Genetic Engineering by Isil Aksan Kurnaz Pdf

Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book—Techniques in Genetic Engineering—IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and genomes; however, in general it is assumed that the reader has a background on these key issues. Techniques in Genetic Engineering briefly introduces some common genetic engineering techniques and focuses on how to approach different real-life problems using a combination of these key issues. Although not an exhaustive review of these techniques, basic information includes core concepts such as DNA, RNA, protein, genes, and genomes. It is assumed that the reader has background on these key issues. The book provides sufficient background and future perspectives for the readers to develop their own experimental strategies and innovations. This easy-to-follow book presents not only the theoretical background of molecular techniques, but also provides case study examples, with some sample solutions. The book covers basic molecular cloning procedures; genetic modification of cells, including stem cells; as well as multicellular organisms, using problem-based case study examples.

Author : D. A. Hopwood
Publisher : Unknown
Page : 378 pages
File Size : 54,8 Mb
Release : 1985
Category : Gene expression
ISBN : UOM:39015027151615

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Genetic Manipulation of Streptomyces by D. A. Hopwood Pdf

Author : Susan Carson,Heather B. Miller,Sue Carson,D. Scott Witherow
Publisher : Academic Press
Page : 227 pages
File Size : 40,6 Mb
Release : 2011-11-07
Category : Science
ISBN : 9780123855442

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Molecular Biology Techniques by Susan Carson,Heather B. Miller,Sue Carson,D. Scott Witherow Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project” approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : P. M. Swamy
Publisher : Rastogi Publications
Page : 638 pages
File Size : 49,8 Mb
Release : 2008
Category : Biotechnology
ISBN : 8171339182

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Laboratory Manual on Biotechnology by P. M. Swamy Pdf

Author : Jennifer A. Doudna,Prashant Mali
Publisher : Unknown
Page : 0 pages
File Size : 51,7 Mb
Release : 2016
Category : Genetics
ISBN : 1621821307

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CRISPR-Cas by Jennifer A. Doudna,Prashant Mali Pdf

CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.

Author : Ronald W. Davis,David Botstein,John R. Roth
Publisher : Unknown
Page : 254 pages
File Size : 51,5 Mb
Release : 1982
Category : Electronic
ISBN : OCLC:311634810

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Advanced Bacterial Genetics by Ronald W. Davis,David Botstein,John R. Roth Pdf

Author : David A. Hopwood
Publisher : Unknown
Page : 356 pages
File Size : 55,6 Mb
Release : 1985-01-01
Category : Science
ISBN : 0879699930

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Genetic Manipulation of Streptomyces by David A. Hopwood Pdf

Author : Robert M. Horton,Robert C. Tait
Publisher : Garland Science
Page : 219 pages
File Size : 54,6 Mb
Release : 1998
Category : Science
ISBN : 1898486050

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Genetic Engineering with PCR by Robert M. Horton,Robert C. Tait Pdf

This is the first laboratory manual to focus on synthetic uses of PCR. PCR-mediated sequence manipulation gives the genetic engineer such precise control over construction, mutation, and recombination of sequences that it represents a new generation of DNA technology. There are many applications, innovations, and refinements of the use of PCR for synthetic purposes. This entire volume is devoted to cover the topic in breadth and depth and includes a variety of approaches for mutagenesis, recombination, and the construction of synthetic or semi-synthetic genes.

Author : Theodore Friedmann,John J. Rossi
Publisher : CSHL Press
Page : 769 pages
File Size : 54,5 Mb
Release : 2007
Category : Gene Transfer Techniques
ISBN : 9780879697655

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Gene Transfer by Theodore Friedmann,John J. Rossi Pdf

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.

Author : Judith W. Zyskind,Sanford I. Bernstein
Publisher : Elsevier
Page : 208 pages
File Size : 40,8 Mb
Release : 2014-05-12
Category : Science
ISBN : 9781483220970

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Recombinant DNA Laboratory Manual by Judith W. Zyskind,Sanford I. Bernstein Pdf

Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.

Author : Michael Kriegler
Publisher : Springer
Page : 248 pages
File Size : 44,7 Mb
Release : 1990-06-18
Category : Science
ISBN : 9781349118915

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Gene Transfer and Expression by Michael Kriegler Pdf

A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.