Crispr Cas

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CRISPR-Cas Methods

M. Tofazzal Islam,Kutubuddin Ali Molla
CRISPR Cas Methods Book PDF

Author : M. Tofazzal Islam,Kutubuddin Ali Molla
Publisher : Humana
Page : 397 pages
File Size : 40,9 Mb
Release : 2021-07-31
Category : Science
ISBN : 1071616560

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CRISPR-Cas Methods by M. Tofazzal Islam,Kutubuddin Ali Molla Pdf

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

CRISPR/Cas Genome Editing

Anjanabha Bhattacharya,Vilas Parkhi,Bharat Char
CRISPR Cas Genome Editing Book PDF

Author : Anjanabha Bhattacharya,Vilas Parkhi,Bharat Char
Publisher : Springer Nature
Page : 232 pages
File Size : 44,8 Mb
Release : 2020-12-11
Category : Science
ISBN : 9783030420222

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CRISPR/Cas Genome Editing by Anjanabha Bhattacharya,Vilas Parkhi,Bharat Char Pdf

This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.

CRISPR-Cas

Jennifer A. Doudna,Prashant Mali
CRISPR Cas Book PDF

Author : Jennifer A. Doudna,Prashant Mali
Publisher : Unknown
Page : 0 pages
File Size : 45,6 Mb
Release : 2016
Category : Genetics
ISBN : 1621821307

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CRISPR-Cas by Jennifer A. Doudna,Prashant Mali Pdf

CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.

Genome Engineering via CRISPR-Cas9 System

Vijai Singh,Pawan K. Dhar
Genome Engineering via CRISPR Cas9 System Book PDF

Author : Vijai Singh,Pawan K. Dhar
Publisher : Academic Press
Page : 0 pages
File Size : 48,9 Mb
Release : 2020-02-21
Category : Science
ISBN : 0128181400

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Genome Engineering via CRISPR-Cas9 System by Vijai Singh,Pawan K. Dhar Pdf

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.

CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases

Luis María Vaschetto
CRISPR Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases Book PDF

Author : Luis María Vaschetto
Publisher : CRC Press
Page : 315 pages
File Size : 41,7 Mb
Release : 2022-01-31
Category : Mathematics
ISBN : 9781000540833

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CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases by Luis María Vaschetto Pdf

The CRISPR-Cas9 genome-editing system is creating a revolution in the science world. In the laboratory, CRISPR-Cas9 can efficiently be used to target specific genes, correct mutations and regulate gene expression of a wide array of cells and organisms, including human cells. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases is a unique reading material for college students, academicians, and other health professionals interested in learning about the broad range of applications of CRISPR/Cas9 genetic scissors. Some topics included in this book are: the role of the CRISPR/Cas9 system in neuroscience, gene therapy, epigenome editing, genome mapping, cancer, virus infection control strategies, regulatory challenges and bioethical considerations.

CRISPR Gene Editing

Yonglun Luo
CRISPR Gene Editing Book PDF

Author : Yonglun Luo
Publisher : Unknown
Page : 362 pages
File Size : 54,8 Mb
Release : 2019
Category : CRISPR-associated protein 9
ISBN : 1493991701

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CRISPR Gene Editing by Yonglun Luo Pdf

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Plant Genome Editing with CRISPR Systems

Yiping Qi
Plant Genome Editing with CRISPR Systems Book PDF

Author : Yiping Qi
Publisher : Springer Nature
Page : 360 pages
File Size : 49,9 Mb
Release : 2019-01-04
Category : Science
ISBN : 9781493989911

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Plant Genome Editing with CRISPR Systems by Yiping Qi Pdf

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

CRISPR-Cas Systems

Rodolphe Barrangou,John van der Oost
CRISPR Cas Systems Book PDF

Author : Rodolphe Barrangou,John van der Oost
Publisher : Springer Science & Business Media
Page : 300 pages
File Size : 42,9 Mb
Release : 2012-12-13
Category : Science
ISBN : 9783642346576

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CRISPR-Cas Systems by Rodolphe Barrangou,John van der Oost Pdf

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases

Luis María Vaschetto
CRISPR Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases Book PDF

Author : Luis María Vaschetto
Publisher : CRC Press
Page : 268 pages
File Size : 44,7 Mb
Release : 2022-01-31
Category : Mathematics
ISBN : 9781000540819

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CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases by Luis María Vaschetto Pdf

The CRISPR-Cas9 genome-editing system is creating a revolution in the science world. In the laboratory, CRISPR-Cas9 can efficiently be used to target specific genes, correct mutations and regulate gene expression of a wide array of cells and organisms, including human cells. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases is a unique reading material for college students, academicians, and other health professionals interested in learning about the broad range of applications of CRISPR/Cas9 genetic scissors. Some topics included in this book are: the role of the CRISPR/Cas9 system in neuroscience, gene therapy, epigenome editing, genome mapping, cancer, virus infection control strategies, regulatory challenges and bioethical considerations.

The CRISPR/Cas Tool Kit for Genome Editing

Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan
The CRISPR Cas Tool Kit for Genome Editing Book PDF

Author : Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan
Publisher : Springer Nature
Page : 342 pages
File Size : 54,5 Mb
Release : 2022-01-01
Category : Medical
ISBN : 9789811663055

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The CRISPR/Cas Tool Kit for Genome Editing by Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan Pdf

This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.

CRISPR Genome Surgery in Stem Cells and Disease Tissues

Stephen H. Tsang
CRISPR Genome Surgery in Stem Cells and Disease Tissues Book PDF

Author : Stephen H. Tsang
Publisher : Academic Press
Page : 138 pages
File Size : 52,6 Mb
Release : 2021-10-20
Category : Science
ISBN : 9780128178775

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CRISPR Genome Surgery in Stem Cells and Disease Tissues by Stephen H. Tsang Pdf

CRISPR Genome Surgery in Stem Cells and Disease Tissues focuses uniquely on the clinical applications of CRISPR/Cas9 based technology. Topics include the latest advances in gene editing and its translational applications to various diseases, including retinal degenerative disease, recessively inherited diseases, and dominantly inherited diseases, to name a few. The book's target audience includes researchers, students, clinicians and the general public. This space that is not currently served by any existing resource, so this publication fills a gap in current literature. Provides a thorough review of CRISPR-Cas9, from discovery to therapy Covers the latest advances in gene editing and its translational applications to various diseases Written by global leaders in the fields of gene editing and stem cell therapy

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part A

Anonim
Reprogramming the Genome Applications of CRISPR Cas in non mammalian systems part A Book PDF

Author : Anonim
Publisher : Academic Press
Page : 244 pages
File Size : 44,9 Mb
Release : 2021-03-28
Category : Science
ISBN : 9780323853224

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Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part A by Anonim Pdf

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more. Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics. Offers a basic understanding and clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, and many more

Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy

Anonim
Reprogramming the Genome CRISPR Cas based Human Disease Therapy Book PDF

Author : Anonim
Publisher : Academic Press
Page : 394 pages
File Size : 55,7 Mb
Release : 2021-06-12
Category : Science
ISBN : 9780323853248

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Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy by Anonim Pdf

Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy, presents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 is a key technology for targeted genome editing and regulation in a number of organisms including mammalian cells. It is a rapid, simple, and cost-effective solution. CRISPR-Cas system has recently gained much scientific and public attention. This volume covers CRISPR-Cas9 based mammalian genome editing, creating disease models, cancer therapy, neurological, heredity, blood disorders, defective gene correction, stem cells therapy, epigenetic modifications, patents, ethics, biosafety and regulatory issues challenges and opportunities. This book is a key source of information on mammalian genome editing available in a single volume. This book will be useful for beginners in mammalian genome editing and also students, researchers, scientists, policymakers, clinicians and stakeholders interested in genome editing in several areas. Offers basic understanding and a clear picture of mammalian genome editing through CRISPR-Cas systems Discusses how to create mammalian disease models, stem cell modification, epigenetic modifications, correction of defective gene in blood disorders, heredity, neurological disorders and many more Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology, translational sciences, neurobiology, neurology, cancer, stem cells, and many more

Advances in CRISPR/Cas and Related Technologies

Dipanjan Ghosh
Advances in CRISPR Cas and Related Technologies Book PDF

Author : Dipanjan Ghosh
Publisher : Academic Press
Page : 284 pages
File Size : 53,9 Mb
Release : 2021-03-05
Category : Science
ISBN : 9780128215913

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Advances in CRISPR/Cas and Related Technologies by Dipanjan Ghosh Pdf

Advances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advancement in the genome editing techniques. Associated chapters in this new release include Challenges for Therapeutic application of CRISPR Cas techniques, Mitochondrial DNA modification by CRISPR Cas System: Challenges and future direction, Trends in CRISPR Cas technology application in cancer, Modified CRISPR-Cas for next generation application, Application of CRISPR Cas in Synthetic Biology: Challenges and Scopes, History of CRISPR Cas system from bacterial Adaptive Immune System to research application, and more. Covers the Cas9 protein modification for reduced off-target effect Includes discussions on Cas9 utilization for Metabolic Engineering Provides information on the use of Cas9 for targeted delivery in therapeutic application

CRISPR

Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fineran
CRISPR Book PDF

Author : Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fineran
Publisher : Humana
Page : 0 pages
File Size : 45,5 Mb
Release : 2016-10-09
Category : Medical
ISBN : 1493954288

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CRISPR by Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fineran Pdf

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.