Linear Dichroism Spectroscopy

Author : Bengt Nordén,Alison Rodger,Tim Dafforn
Publisher : Royal Society of Chemistry
Page : 305 pages
File Size : 43,6 Mb
Release : 2019-05-02
Category : Science
ISBN : 9781788018210

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Linear Dichroism and Circular Dichroism by Bengt Nordén,Alison Rodger,Tim Dafforn Pdf

This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.

Author : Alison Rodger,Bengt Nordén
Publisher : Oxford University Press, USA
Page : 170 pages
File Size : 49,8 Mb
Release : 1997
Category : Science
ISBN : 019855897X

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Circular Dichroism and Linear Dichroism by Alison Rodger,Bengt Nordén Pdf

This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.

Author : Bonnie Ann Wallace,Robert William Janes
Publisher : IOS Press
Page : 244 pages
File Size : 48,6 Mb
Release : 2009
Category : Medical
ISBN : 9781607500001

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Modern Techniques for Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy by Bonnie Ann Wallace,Robert William Janes Pdf

Presents an account of circular dichroism (CD) spectroscopy and its application to structural biology. This book covers the methods of synchrotron radiation circular dichroism (SRCD) and linear dichroism (LD).

Author : Bengt Nordén
Publisher : Lund University Press
Page : 278 pages
File Size : 44,7 Mb
Release : 1977
Category : Science
ISBN : CORNELL:31924004749788

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Linear Dichroism Spectroscopy by Bengt Nordén Pdf

Author : B. Samori',E.W. Thulstrup
Publisher : Springer Science & Business Media
Page : 576 pages
File Size : 40,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400930391

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Polarized Spectroscopy of Ordered Systems by B. Samori',E.W. Thulstrup Pdf

Ordered systems exhibit physical properties and behavior unknown in media where structural ordering and organization do not take place. In ordered systems special correlations between molecules exist and the results are remarkable properties: the functional order of biological systems, the electrooptical and mechanical proper ties of liquid crystalline materials and stretched polymers are just a few examples. New methods and techniques in optical spectroscopy have recently been developed to study ordered systems and guest molecules. This stimulated the organization of a NATO Advanced Study Institute bringing together chemists and physicists from optical spectroscopy, materials science, and biology. Thereby a unifying and interdisciplinary survey of possible applications of spectroscopy with polarized light to ordered systems, such as liquid crystals, stretched polymers, polymeric liquid crystals, and membranes, was achieved. The interdisciplinary approach of the meeting is reflected in the book. Different aspects of the same topic are often treated in several chapters all through the book. Therefore, each reader should look for the contributions which serves his needs, even if this means that some chapters will be skipped. The Advanced Study Institute, "New Developments in Polarized Spectroscopy of Ordered Systems", was the first scientific event of the celebrations of the 900th anniversary of the University of Bologna. The international and multidisciplinary approach of this ASI well converged in the tradition of the "Studium" at Bologna.

Author : W. Roy Mason
Publisher : John Wiley & Sons
Page : 237 pages
File Size : 48,7 Mb
Release : 2007-07-09
Category : Science
ISBN : 9780470139226

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Magnetic Circular Dichroism Spectroscopy by W. Roy Mason Pdf

The book is a technical guide for chemists and spectroscopists, and presents a concise description of magnetic circular dichroism (MCD) spectroscopy and how it has advanced the interpretation of molecular electronic spectra. Provides a practical guide to utilizing MCD spectroscopy for chemists starting in the field Written by an expert with over twenty years of experience in the field Helps the reader to visualize the optical spectroscopic effects presented by MCD measurements Includes practical considerations for experimental MCD measurements based on the author's experience Written as a general discussion of the subject matter, with illustrative examples provided and discussed in the case studies to show the breadth of application of MCD measurements.

Author : Vasudevan Ramesh
Publisher : John Wiley & Sons
Page : 576 pages
File Size : 49,5 Mb
Release : 2019-06-10
Category : Science
ISBN : 9781119483960

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Biomolecular and Bioanalytical Techniques by Vasudevan Ramesh Pdf

An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.

Author : Jasmin Wallace
Publisher : Unknown
Page : 195 pages
File Size : 52,6 Mb
Release : 2015
Category : SCIENCE
ISBN : 1634838009

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Circular and Linear Dichroism by Jasmin Wallace Pdf

This book provides readers with the latest developments in circular and linear dichroism research. Topics covered include the visible circular and linear magnetic dichroism in manganite thin films; the optical and energetic contributions of DNA secondary motifs to the trombin aptamer quadruplex and a review of the current theoretical understanding of the x-ray magnetic circular and linear dichroism (XMCD, XLD) of diluted magnetic semiconductors (DMS).

Author : Erik W. Thulstrup,Josef Michl
Publisher : John Wiley & Sons
Page : 180 pages
File Size : 42,5 Mb
Release : 1989-12-03
Category : Science
ISBN : 0471190578

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Elementary Polarization Spectroscopy by Erik W. Thulstrup,Josef Michl Pdf

This book offers an elementary introduction to optical spectroscopy with polarized light. It is written at a level suitable for undergraduate students in chemistry and undergraduate or graduate students in related disciplines such as biochemistry, biology, chemical engineering and materials science. It emphasizes the qualitative concepts and deemphasizes mathematics, yet provides sufficient information and practical hints for experiments. With this book in hand, anyone who can measure an ordinary infrared, ultraviolet or visible absorption or fluorescence emission spectrum can measure a polarized one as well and learn far more about the sample.

Author : Nagao Kobayashi,Atsuya Muranaka
Publisher : Royal Society of Chemistry
Page : 216 pages
File Size : 42,9 Mb
Release : 2011-11-10
Category : Science
ISBN : 9781849732932

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Circular Dichroism and Magnetic Circular Dichroism Spectroscopy for Organic Chemists by Nagao Kobayashi,Atsuya Muranaka Pdf

CD and MCD spectroscopy can provide key information about the conformations and electronic states of chromophore containing molecules. However, the theory has remained too challenging and inaccessible for many organic chemists and biochemists and only a few researchers have carried out detailed quantitative analyses of their spectral data. This is not surprising as people who excel at spectroscopic theory usually lack the skills set required to design and synthesise the molecules that would be most appropriate for describing and explaining the theory of CD and MCD spectroscopy. Most of the books that have been written on the subject have, therefore, been based on very dense sets of mathematical equations. This timely book rectifies that situation by summarizing the relationship between the different types of spectra and by describing in detail the qualitative and quantitative methods which can readily be used to analyse CD and MCD spectral data. During the last decade the authors have successfully synthesized several molecules to illustrate key points related to the theory of CD and MCD spectroscopy, resulting in this definitive book providing key practical knowledge in a readily accessible style. It is aimed primarily at organic chemists and biochemists and is required reading for researchers active in the field. In the introduction, the book describes the types of information that can be derived from CD and MCD spectroscopy. After a detailed explanation of the theory of electronic absorption spectroscopy, it then provides practical in depth examples of the various analytical methods that can be carried out with CD and MCD spectral data. This makes the theory of these techniques much more accessible for researchers who do not specialise in physical chemistry. The book concludes by briefly summarizing the key mathematical expressions associated with each technique.

Author : G.D. Fasman
Publisher : Springer Science & Business Media
Page : 739 pages
File Size : 46,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781475725087

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Circular Dichroism and the Conformational Analysis of Biomolecules by G.D. Fasman Pdf

''Excellent and very timely....It will undoubtedly become a standard reference for the application of circular dichroism (CD) to biomolecules.'' --- Quarterly Review of Biology, March 1997 ''[T]estament to the book's utility is the fact that during the course of my review I had to 'rescue' it from the desks of graduate students on an almost daily basis. In summary, this is a great book.'' --- American Scientist ''Well documented chapters provide a very good insight into the problems surrounding the conformation of biomacromolecules...An indispensible source of information.'' --- Nahrung, 42(2), 1998 Renowned experts present the first state-of-the-art description of circular dichroism spectroscopy (CD). Chapters present in-depth discussions of the history of the field, the theory of CD for application to globular proteins, membrane proteins, peptides, nucleic acids and their interactions, carbohydrates, and instrumentation. Discussions also feature new techniques using synchrotron radiation, vibrational Raman optical activity, and vibrational CD. More than 250 illustrations supplement the text.

Author : Jeroen A. van Bokhoven,Carlo Lamberti
Publisher : John Wiley & Sons
Page : 940 pages
File Size : 55,6 Mb
Release : 2016-03-21
Category : Science
ISBN : 9781118844236

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X-Ray Absorption and X-Ray Emission Spectroscopy by Jeroen A. van Bokhoven,Carlo Lamberti Pdf

During the last two decades, remarkable and often spectacular progress has been made in the methodological and instrumental aspects of x–ray absorption and emission spectroscopy. This progress includes considerable technological improvements in the design and production of detectors especially with the development and expansion of large-scale synchrotron reactors All this has resulted in improved analytical performance and new applications, as well as in the perspective of a dramatic enhancement in the potential of x–ray based analysis techniques for the near future. This comprehensive two-volume treatise features articles that explain the phenomena and describe examples of X–ray absorption and emission applications in several fields, including chemistry, biochemistry, catalysis, amorphous and liquid systems, synchrotron radiation, and surface phenomena. Contributors explain the underlying theory, how to set up X–ray absorption experiments, and how to analyze the details of the resulting spectra. X-Ray Absorption and X-ray Emission Spectroscopy: Theory and Applications: Combines the theory, instrumentation and applications of x-ray absorption and emission spectroscopies which offer unique diagnostics to study almost any object in the Universe. Is the go-to reference book in the subject for all researchers across multi-disciplines since intense beams from modern sources have revolutionized x-ray science in recent years Is relevant to students, postdocurates and researchers working on x-rays and related synchrotron sources and applications in materials, physics, medicine, environment/geology, and biomedical materials

Author : Prasad L. Polavarapu
Publisher : CRC Press
Page : 298 pages
File Size : 44,9 Mb
Release : 2016-10-03
Category : Science
ISBN : 9781315357195

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Chiroptical Spectroscopy by Prasad L. Polavarapu Pdf

This book details chiroptical spectroscopic methods: electronic circular dichroism (ECD), optical rotatory dispersion (ORD), vibrational circular dichroism (VCD), and vibrational Raman optical activity (VROA). For each technique, the text presents experimental methods for measurements and theoretical methods for analyzing the experimental data. It also includes a set of experiments that can be adopted for undergraduate teaching laboratories. Each chapter is written in an easy-to-follow format for novice readers, with necessary theoretical formalism in appendices for advanced readers.

Author : Vasilis G. Gregoriou,Mark S. Braiman
Publisher : CRC Press
Page : 448 pages
File Size : 55,9 Mb
Release : 2005-11-14
Category : Science
ISBN : 9781420027549

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Vibrational Spectroscopy of Biological and Polymeric Materials by Vasilis G. Gregoriou,Mark S. Braiman Pdf

Used primarily for characterizing polymers and biological systems, vibrational spectroscopy continues to uncover structural information pertinent to a growing number of applications. Vibrational Spectroscopy of Biological and Polymeric Materials compiles the latest developments in advanced infrared and Raman spectroscopic techniques that are

Author : Peter R. Griffiths,James A. De Haseth
Publisher : John Wiley & Sons
Page : 704 pages
File Size : 46,5 Mb
Release : 2007-03-16
Category : Science
ISBN : 0470106298

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Fourier Transform Infrared Spectrometry by Peter R. Griffiths,James A. De Haseth Pdf

A bestselling classic reference, now expanded and updated to cover the latest instrumentation, methods, and applications The Second Edition of Fourier Transform Infrared Spectrometry brings this core reference up to date on the uses of FT-IR spectrometers today. The book starts with an in-depth description of the theory and current instrumentation of FT-IR spectrometry, with full chapters devoted to signal-to-noise ratio and photometric accuracy. Many diverse types of sampling techniques and data processing routines, most of which can be performed on even the less expensive instruments, are then described. Extensively updated, the Second Edition: * Discusses improvements in optical components * Features a full chapter on FT Raman Spectrometry * Contains new chapters that focus on different ways of measuring spectra by FT-IR spectrometry, including fourteen chapters on such techniques as microspectroscopy, internal and external reflection, and emission and photoacoustic spectrometry * Includes a new chapter introducing the theory of vibrational spectrometry * Organizes material according to sampling techniques Designed to help practitioners using FT-IR capitalize on the plethora of techniques for modern FT-IR spectrometry and plan their experimental procedures correctly, this is a practical, hands-on reference for chemists and analysts. It's also a great resource for students who need to understand the theory, instrumentation, and applications of FT-IR.