Liposomes In Gene Delivery

Author : Danilo D. Lasic
Publisher : CRC Press
Page : 326 pages
File Size : 41,8 Mb
Release : 1997-03-13
Category : Medical
ISBN : 0849331099

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Liposomes in Gene Delivery by Danilo D. Lasic Pdf

Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. Liposomes in Gene Delivery covers both-molecular biologists will gain a basic knowledge of lipids, liposomes, and other gene delivery vehicles; lipid and drug delivery scientists will better understand DNA, molecular biology, and DNA manipulation. Topics include an introduction to nucleic acids, a theoretical description of DNA, recombinant technology, lipids and liposomes, stability and interaction properties of lipids and liposomes, complexation of lipids and liposomes with DNA plasmids, gene expression of genosomes in various models, structure-activity relationships, and transfection models. This is an excellent introductory text for graduate students, scientists, and researchers in molecular and cell biology, genetics, biochemistry, physical chemistry, colloid science, pharmacology, molecular science, and medicine.

Author : Danilo D. Lasic
Publisher : CRC Press
Page : 326 pages
File Size : 43,8 Mb
Release : 2019-07-23
Category : Science
ISBN : 9780429605727

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Liposomes in Gene Delivery by Danilo D. Lasic Pdf

Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. Liposomes in Gene Delivery covers both-molecular biologists will gain a basic knowledge of lipids, liposomes, and other gene delivery vehicles; lipid and drug delivery scientists will better understand DNA, molecular biology, and DNA manipulation. Topics include an introduction to nucleic acids, a theoretical description of DNA, recombinant technology, lipids and liposomes, stability and interaction properties of lipids and liposomes, complexation of lipids and liposomes with DNA plasmids, gene expression of genosomes in various models, structure-activity relationships, and transfection models. This is an excellent introductory text for graduate students, scientists, and researchers in molecular and cell biology, genetics, biochemistry, physical chemistry, colloid science, pharmacology, molecular science, and medicine.

Author : Danilo D. Lasic,Yechezkel Barenholz
Publisher : CRC Press
Page : 611 pages
File Size : 42,8 Mb
Release : 2019-07-18
Category : Science
ISBN : 9781000697636

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Handbook of Nonmedical Applications of Liposomes by Danilo D. Lasic,Yechezkel Barenholz Pdf

First published in 1996, liposomes have become an important model in fundamental biomembrane research, including biophysical, biochemical, and cell biological studies of membranes and cell function. They are thoroughly studied in several applications, such as drug delivery systems in medical applications and as controlled release systems, microencapsulating media, signal carriers, support matrices, and solubilizers in other applications. While medical applications have been extensively reviewed in recent literature, there is a need for easily accessible information on applications for liposomes beyond pharmacology and medicine. The Handbook of Nonmedical Applications of Liposomes fills this void.This unique new handbook series presents recent developments in the use of liposomes in many scientific disciplines, from studies on the origin of life, protein function, and vesicle shapes, to applications in cosmetics, diagnostics, ecology, bioreclamation, and the food industry. In these volumes many of the top experts contribute extensive reviews of their work.

Author : Anonim
Publisher : Elsevier
Page : 635 pages
File Size : 45,9 Mb
Release : 2003-12-18
Category : Science
ISBN : 9780080497129

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Liposomes, Part C by Anonim Pdf

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology liposomes are also used in clinical applications such as drug delivery and virus studies. *Liposomes in Immunology *Liposomes in Diagnostics *Liposomes in Gene Delivery and Gene Therapy

Author : G. Dickson
Publisher : Springer Science & Business Media
Page : 418 pages
File Size : 49,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401105477

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Molecular and Cell Biology of Human Gene Therapeutics by G. Dickson Pdf

advanced metastatic disease of solid tumors, dictates that each tumor mass, indeed each individual metastasis, will have a unique antigen and cytokine environment and hence unique response to immune modu lation. A differential response to immunotherapy is thus inevitable. 4. Many of the human trials described are not randomized and report survival or response against historical controls. Most tumors described are immunogenic human tumors: renal cell cancer and melanoma are most common. In order to avoid the well-described inter-patient vari ation and rare incidence of spontaneous response among patient samples as well as selection bias and changes in practice over time, randomized trials are required. 5. Immunological treatment is unlike conventional chemotherapy in its endpoint. Most chemotherapeutic regimes require a complete response or a good partial response for cure or good palliation. There are now many cases where immunotherapy has provided long-term palliation without massive tumor reduction. Immunity may be stimulated to a degree which holds tumorigenicity in check and most importantly, pro vides good palliation for the patient in a manner that differs essentially from chemotherapy.

Author : Ching-Yi Chow
Publisher : Unknown
Page : 540 pages
File Size : 53,5 Mb
Release : 1995
Category : Electronic
ISBN : WISC:89052515285

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In Vitro Studies of Cationic Liposome Mediated Gene Delivery by Ching-Yi Chow Pdf

Author : Arabinda Chaudhuri
Publisher : Unknown
Page : 300 pages
File Size : 52,8 Mb
Release : 2021-11-29
Category : Electronic
ISBN : 1788015517

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Liposomes by Arabinda Chaudhuri Pdf

Liposomal drug and gene delivery systems have found success in clinical applications due to their biodegradable, biocompatible, non-toxic, and non-immunogenic nature. To underscore their importance in healthcare, in the fight against SARS-CoV-2 virus, lipid-based nanoparticular formulations of mRNA and DNA based vaccines have been developed via academic and industry collaboration. Aimed at newcomers and established researchers alike, this book provides a comprehensive coverage of the field. Each chapter emphasizes design principles and concludes with highlighting unmet translational challenges. Topics covered include up-scaling liposome preparation, FDA approved liposomal drug delivery systems (LDDS) for cancer and non-cancerous diseases; liposomes in non-viral gene therapy; receptor selective LDDS for targeted cancer therapy; liposomal delivery of DNA and mRNA vaccines and liposomes for combination therapy. Whilst there are many review articles, this book brings together information and explains the complex science in an accessible manner.Written for an interdisciplinary audience, this book is an up-to-date resource for this important and vibrant research field.

Author : Wan-Liang Lu,Xian-Rong Qi
Publisher : Springer
Page : 0 pages
File Size : 46,9 Mb
Release : 2021-07-01
Category : Technology & Engineering
ISBN : 3662493187

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Liposome-Based Drug Delivery Systems by Wan-Liang Lu,Xian-Rong Qi Pdf

This volume describes the protocols for fabrication of liposomal drug delivery system, and consists of two parts. The first part emphasizes the basic protocols and concepts for fabrication of drug-loaded liposomes. For new investigators, laying the groundwork is the first step. This part focuses on the fabrication details in liposomes formulations, which are the so-called small tricks while usually not disclosed in mostly published research articles. However, these processing details are crucial for the preparation of liposomes. And the second part focuses on the strategies in modified and/or functionalized liposomes to adapt to pathophysiological environment, as well as their application in treating diseases. As new analytical and synthetic technologies become available, and improved understanding of pathophysiology, various functionalized drug liposomes are developing to fit the requirements of different therapeutic purposes, exhibiting a broad range of applications. Accordingly, the objectives of this part are aimed at deeply unearthing the formulation of drug-loaded liposomes, describing the detailed operation of liposomal formulation, and further demonstrating their potential applications. Therefore, this volume shows the features of experimental report or protocol for proving a guide to scientists, research students, and young investigators in pharmaceutical enterprises.

Author : Gregory Gregoriadis
Publisher : CRC Press
Page : 348 pages
File Size : 42,9 Mb
Release : 2016-04-19
Category : Medical
ISBN : 9780849397264

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Liposome Technology by Gregory Gregoriadis Pdf

Liposome Technology, Volume I: Liposome Preparation and Related Techniques, Third Edition, is a thoroughly updated and expanded new edition of a classic text in the field. Including step-by-step technical details, Volume I illustrates numerous methods for liposome preparation and auxiliary techniques necessary for the stabilization and characteriza

Author : Alexander V. Kabanov,Philip L. Felgner,Leonard W. Seymour
Publisher : Wiley-Blackwell
Page : 492 pages
File Size : 52,7 Mb
Release : 1998-05-29
Category : Medical
ISBN : UOM:39015042032857

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Self-Assembling Complexes for Gene Delivery by Alexander V. Kabanov,Philip L. Felgner,Leonard W. Seymour Pdf

Recent years have witnessed an explosion of activity in the field of gene therapy. Following advances in our understanding of the molecular basis of disease, hopes are high that the tremendous potential market for drugs employing antisense nucleotides and genes will one day be fulfilled. However, many obstacles remain, not least problems in the technology of gene delivery systems. Much of today's research focuses on non-viral approaches to gene delivery. Of particular importance are supramolecular complexes formed between DNA and various natural and synthetic polymers and lipids, otherwise known as 'self-assembling complexes for gene delivery'. In view of this fact, the editors of this volume have assembled an international team of contributors to present up-to-date reviews of the major chemical, biological and clinical aspects of such gene delivery vectors. Reflecting the diversity of research in this field, this book comprehensively covers: * the principles of self-assembly * natural mechanisms for gene delivery to cells * cationic lipids and liposomes * polyelectrolyte DNA complexes * systemic biodistribution of drug delivery systems * targeting of conjugates for gene delivery * new approaches to gene delivery * clinical evaluation Self-assembling Complexes for Gene Delivery is an essential reference for all professionals with an interest in gene and antisense therapy or novel drug delivery systems, including medicinal and pharmaceutical chemists, clinicians, human geneticists, molecular biologists and pharmacologists.

Author : Danilo D. Lasic,Yechezkel Barenholz
Publisher : CRC-Press
Page : 0 pages
File Size : 50,7 Mb
Release : 1996-01-09
Category : Science
ISBN : 0849340136

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Handbook of Nonmedical Applications of Liposomes by Danilo D. Lasic,Yechezkel Barenholz Pdf

Liposomes have become an important model in fundamental biomembrane research, including biophysical, biochemical, and cell biological studies of membranes and cell function. They are thoroughly studied in applications, such as drug delivery systems in medical applications and as controlled release systems, microencapsulating media, signal carriers, support matrices, and solubilizers in other applications. While medical applications have been extensively reviewed in recent literature, there is a need for easily accessible information on applications for liposomes beyond pharmacology and medicine.

Author : Sophia G. Antimisiaris
Publisher : Elsevier
Page : 698 pages
File Size : 40,8 Mb
Release : 2024-04-15
Category : Medical
ISBN : 9780443154928

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Liposomes in Drug Delivery by Sophia G. Antimisiaris Pdf

Liposomes in Drug Delivery: What, Where, How and When to Deliver is a concise, well-structured reference covering all the important issues related to the potential of this technology. Organized to provide practical information to researchers from any discipline with a particular therapeutic or bio-active substance to deliver, this book helps readers understand if liposomes can be of benefit for their particular need, what is the best type of liposome to use according to what needs to be delivered, where/when to deliver it, and how to design/prepare/characterize/investigate/optimize liposome properties for a particular application. The book is structured in Four parts. The first covers in a concise but in-depth way, what liposomes are, which are the liposome types, advantages/disadvantages, and what is their stability, characterization methods, in vitro stability, and in vivo fate after different administration methods (routes). The second part focuses on the different mechanisms for liposomal drug delivery. Methodologies/technologies for manipulation of liposome structure/properties in order to design liposomes for particular delivery applications. Specific roadmaps for liposome design are discussed, including components to incorporate in liposomes for specific types of encapsulated molecules or specific routes of administration. The third part covers liposome applications for drug delivery. It focuses on specific delivery considerations for particular diseases. Finally, the fourth part covers methods of liposome fabrication. Focuses on key information - What, Where, How and When to deliver – needed for drug delivery researchers Covers all aspects of liposomes in drug delivery in one single volume Guides researchers through the decision process on whether and what liposomes are most applicable to their particular interest

Author : Mien-Chie Hung,Leaf Huang,Ernst Wagner
Publisher : Elsevier
Page : 456 pages
File Size : 46,8 Mb
Release : 1999-07-01
Category : Science
ISBN : 0080479774

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Nonviral Vectors for Gene Therapy by Mien-Chie Hung,Leaf Huang,Ernst Wagner Pdf

Gene transfer within humans has been an obstacle until about 10 years ago. At that time, it was found that viral vectors were effective carriers of "healthy genes" into patients' cells. The problem, however, was that viral vectors proved unnecessarily harmful to humans: subjects experienced inflamatory activity and negative immunological responses to the genes. Viral vectors were also unable to meet the needs of the pharmaceutical community: they were not reproducible in large-scale proportions in cost-effective ways. Thus, research was undertaken to find a safer way to transfer genes to patients without jeopardizing the safety of the patient. And so non-viral vectors were discovered. This volume presents the various non-viral vectors currently under development. Although not methodologically oriented, it will provide the necessary details behind the development of the vectors. This information will prove useful to both researchers and clinicians. Key Features * Presents state-of-the art developments of nonviral vectors as tools for modern molecular medicine * Covers all types of nonviral vectors, from molecular structure to therapeutic application Provides a comprehensive review of synthetic vectors * Includes contributions from major investigators and leading experts in the field

Author : William C. Heiser
Publisher : Springer Science & Business Media
Page : 303 pages
File Size : 42,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596492

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Gene Delivery to Mammalian Cells by William C. Heiser Pdf

Experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1 focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. Volume 2 details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Author : Gerard G.M. D'Souza,Hongwei Zhang
Publisher : Springer Nature
Page : 301 pages
File Size : 53,6 Mb
Release : 2023-02-13
Category : Science
ISBN : 9781071629543

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Liposomes by Gerard G.M. D'Souza,Hongwei Zhang Pdf

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.