Membrane Proteins Engineering Purification And Crystallization

Author : Anonim
Publisher : Academic Press
Page : 644 pages
File Size : 46,9 Mb
Release : 2015-04-30
Category : Science
ISBN : 9780128021934

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Membrane Proteins – Engineering, Purification and Crystallization by Anonim Pdf

Membrane Proteins – Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In Enzymology Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Author : Carola Hunte,Gebhard von Jagow,Hermann Schagger
Publisher : Elsevier
Page : 316 pages
File Size : 40,6 Mb
Release : 2003-01-05
Category : Science
ISBN : 9780080536170

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Membrane Protein Purification and Crystallization by Carola Hunte,Gebhard von Jagow,Hermann Schagger Pdf

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins

Author : Anonim
Publisher : Academic Press
Page : 336 pages
File Size : 54,9 Mb
Release : 2009-05-29
Category : Science
ISBN : 0080961592

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Membrane Protein Crystallization by Anonim Pdf

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Author : Hartmut Michel
Publisher : CRC Press
Page : 236 pages
File Size : 51,5 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351079723

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Crystallization of Membrane Proteins by Hartmut Michel Pdf

The precise knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action. It also lays the foundation for rational protein and drug design. The only method to obtain this knowledge is still crystallography. At present, the structures of about 400 proteins are known at or nearly at atomic proteins. However, only two of them are membrane proteins or complexes of the membrane proteins. The reasons for the difference is not the crystals of membrane proteins resists forming special problems when being analysed. The reason is that the membrane proteins resist into forming into well-ordered crystals. The intention of this book is to help to produce well-ordered crystals proteins and to provide guidelines, it is aimed at both biochemists and protein crystallographer‘s.

Author : Gebhard von Jagow
Publisher : Elsevier
Page : 166 pages
File Size : 53,5 Mb
Release : 2013-10-22
Category : Science
ISBN : 9780080571720

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A Practical Guide to Membrane Protein Purification by Gebhard von Jagow Pdf

A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Provides general guidelines and strategies for isolation of membrane proteins Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs Gives special emphasis to new native and denaturing electrophoresis techniques Explains modifications of techniques used for water-soluble proteins

Author : So Iwata
Publisher : Internat'l University Line
Page : 392 pages
File Size : 52,5 Mb
Release : 2003
Category : Science
ISBN : 0963681796

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Methods and Results in Crystallization of Membrane Proteins by So Iwata Pdf

Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 45,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592594009

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Membrane Protein Protocols by Barry S. Selinsky Pdf

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Author : Arnaud Ducruix,Richard Giegé
Publisher : Unknown
Page : 460 pages
File Size : 54,8 Mb
Release : 1999
Category : Science
ISBN : 9780199636785

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Crystallization of Nucleic Acids and Proteins by Arnaud Ducruix,Richard Giegé Pdf

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucleic Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucleic Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.

Author : Isabel Moraes
Publisher : Springer
Page : 184 pages
File Size : 40,9 Mb
Release : 2016-08-23
Category : Science
ISBN : 9783319350721

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The Next Generation in Membrane Protein Structure Determination by Isabel Moraes Pdf

This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.

Author : Roger Harrison
Publisher : CRC Press
Page : 410 pages
File Size : 54,7 Mb
Release : 1993-10-15
Category : Medical
ISBN : 082479009X

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Protein Purification Process Engineering by Roger Harrison Pdf

Offers coverage of the development of protein purification processes for large-scale commercial operations, and addresses process development, scale-up, applications and mathematical descriptions. Technologies currently used at the commercial scale are covered in depth.

Author : Terese M. Bergfors
Publisher : Internat'l University Line
Page : 505 pages
File Size : 47,6 Mb
Release : 2009
Category : Medical
ISBN : 9780972077446

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Protein Crystallization by Terese M. Bergfors Pdf

Author : E. Drioli,Gianluca Di Profio,Efrem Curcio
Publisher : Unknown
Page : 244 pages
File Size : 54,5 Mb
Release : 2015-09-16
Category : Science
ISBN : 1783263326

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Membrane-assisted Crystallization Technology by E. Drioli,Gianluca Di Profio,Efrem Curcio Pdf

This book covers all the basic and applied aspects of crystallization processes based on membrane technology. Synthesis and processing of membrane materials are discussed and reviewed, while mass/heat transport and control are treated in view of the non-reversible thermodynamic principles and statistical thermodynamics. Engineering process design and crystalline materials products properties, and also the relation to other traditional crystallization formats, are analyzed. Advantages, limitations, and future developments are also included in the content, with special emphasis on new fields of applications like microfluidic configurations, controlled proteins (also membrane proteins) crystallization, organic semiconductors single crystals production, and optical materials.

Author : Enrico Drioli,Gianluca Di Profio,Efrem Curcio
Publisher : Imperial College Press
Page : 232 pages
File Size : 46,9 Mb
Release : 2014-09-30
Category : Science
ISBN : 1783263318

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Membrane-assisted Crystallization Technology by Enrico Drioli,Gianluca Di Profio,Efrem Curcio Pdf

This book covers all the basic and applied aspects of crystallization processes based on membrane technology. Synthesis and processing of membrane materials are discussed and reviewed, while mass/heat transport and control are treated in view of the non-reversible thermodynamic principles and statistical thermodynamics. Engineering process design and crystalline materials products properties, and also the relation to other traditional crystallization formats, are analyzed. Advantages, limitations, and future developments are also included in the content, with special emphasis on new fields of applications like microfluidic configurations, controlled proteins (also membrane proteins) crystallization, organic semiconductors single crystals production, and optical materials.

Author : Isabelle Mus-Veteau
Publisher : Humana
Page : 0 pages
File Size : 47,8 Mb
Release : 2023-07-15
Category : Science
ISBN : 1071623702

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Heterologous Expression of Membrane Proteins by Isabelle Mus-Veteau Pdf

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 343 pages
File Size : 41,6 Mb
Release : 2017-07-14
Category : Science
ISBN : 9781498752732

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.