Modified Nucleic Acids

Author : Stefan Jurga,Volker A. Erdmann (Deceased),Jan Barciszewski
Publisher : Springer
Page : 453 pages
File Size : 54,9 Mb
Release : 2016-07-29
Category : Medical
ISBN : 9783319341750

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Modified Nucleic Acids in Biology and Medicine by Stefan Jurga,Volker A. Erdmann (Deceased),Jan Barciszewski Pdf

This volume is comprised of 18 chapters, covering various aspects of DNA modification and RNA modified bases. It also discusses in detail circular RNA, therapeutic oligonucleotides and their different properties. The chemical nature of DNA, RNA, protein and lipids makes these macromolecules easily modifiable, but they are also susceptible to damage from both endogenous and exogenous agents. Alkylation and oxidation show a potential to disrupt the cellular redox equilibrium and cause cellular damage leading to inflammation and even chronic disease. Furthermore, DNA damage can drive mutagenesis and the resulting DNA sequence changes can induce carcinogenesis and cancer progression. Modified nucleosides can occur as a result of oxidative DNA damage and RNA turnover, and are used as markers for various diseases. To function properly some RNA needs to be chemically modified post-transcriptionally. Dysregulation of the RNA-modification pattern or of the levels of the enzymes that catalyze these modifications alters RNA functionality and can result in complex phenotypes, likely due to defects in protein translation. While modifications are best characterized in noncoding ribonucleic acids like tRNA and rRNA, coding mRNAs have also been found to contain modified nucleosides. This book is a valuable resource, not only for graduate students but also researchers in the fields of molecular medicine and molecular biology.

Author : Kazuhiko Nakatani,Yitzhak Tor
Publisher : Springer
Page : 276 pages
File Size : 47,7 Mb
Release : 2016-04-04
Category : Science
ISBN : 9783319271118

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Modified Nucleic Acids by Kazuhiko Nakatani,Yitzhak Tor Pdf

This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.

Author : Ramon Eritja
Publisher : Walter de Gruyter GmbH & Co KG
Page : 380 pages
File Size : 48,8 Mb
Release : 2021-01-18
Category : Science
ISBN : 9783110639537

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Nucleic Acids Chemistry by Ramon Eritja Pdf

This book compiles recent research on the modification of nucleic acids. It covers backbone modifications and conjugation of lipids, peptides and proteins to oligonucleotides and their therapeutic use. Synthesis and application in biomedicine and nanotechnology of aptamers, fluorescent and xeno nucleic acids, DNA repair and artificial DNA are discussed as well.

Author : Jesús Fernández Lucas,María-José Camarasa Rius
Publisher : John Wiley & Sons
Page : 348 pages
File Size : 48,9 Mb
Release : 2019-04-29
Category : Science
ISBN : 9783527343768

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Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives by Jesús Fernández Lucas,María-José Camarasa Rius Pdf

A review of innovative tools for creative nucleic acid chemists that open the door to novel probes and therapeutic agents Nucleic acids continue to gain importance as novel diagnostic and therapeutic agents. With contributions from noted scientists and scholars, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practical reference that includes a wide range of approaches for the synthesis of designer nucleic acids and their derivatives. The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications. This important book: -Presents a timely and topical guide to the synthesis of designer nucleic acids and their derivatives -Addresses the growing market for nucleotide-derived pharmaceuticals used as anti-infectives and chemotherapeutic agents, as well as fungicides and other agrochemicals. -Covers novel methods and the most recent trends in the field -Contains contributions from an international panel of noted scientistics Written for biochemists, medicinal chemists, natural products chemists, organic chemists, and biotechnologists, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practice-oriented guide that reviews innovative methods for the enzymatic as well as non-enzymatic synthesis of nucleic acid species.

Author : Piet Herdewijn
Publisher : Springer Science & Business Media
Page : 436 pages
File Size : 41,5 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592598236

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Oligonucleotide Synthesis by Piet Herdewijn Pdf

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Author : Günter Mayer
Publisher : Humana
Page : 0 pages
File Size : 48,6 Mb
Release : 2015-11-10
Category : Science
ISBN : 1493931962

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Nucleic Acid Aptamers by Günter Mayer Pdf

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

Author : Serge L. Beaucage
Publisher : Current Protocols
Page : 128 pages
File Size : 44,5 Mb
Release : 2000
Category : Science
ISBN : 047124662X

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Current Protocols in Nucleic Acid Chemistry by Serge L. Beaucage Pdf

Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.

Author : Anant R. Kapdi,Debabrata Maiti,Yogesh S. Sanghvi
Publisher : Elsevier
Page : 358 pages
File Size : 54,5 Mb
Release : 2018-06-04
Category : Science
ISBN : 9780128112939

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Palladium-Catalyzed Modification of Nucleosides, Nucleotides and Oligonucleotides by Anant R. Kapdi,Debabrata Maiti,Yogesh S. Sanghvi Pdf

Palladium-Catalyzed Modification of Nucleosides, Nucleotides and Oligonucleotides describes the procedures and protocols related to the modification of nucleosides, nucleotides and oligonucleotides via Pd-mediated cross-coupling processes. The book highlights the growing area of nucleic acid modification and how Pd-mediated coupling reactions can assist this development. Users will find key synthetic protocols for these reactions in this latest volume in the Latest Trends in Palladium Chemistry series. As most of the research in the field of antiviral agents has centered on the use of modified nucleosides that have exhibited promising activity, this book provides an up-to-date reference for both professionals in industry and other interested parties. Provides synthetic routes for useful nucleoside molecules, information otherwise found only through time-consuming literature searches Covers metal-mediated and metal-catalyzed cross coupling processes of nucleosides and related compounds Includes Suzuki-Miyaura, Stille and Sonogashira reactions, as well as C-H bond functionalization Highlights the growing area of nucleic acid modification and how Pd-mediated coupling reactions can assist

Author : Yiyang Dong
Publisher : John Wiley & Sons
Page : 426 pages
File Size : 50,8 Mb
Release : 2019-02-11
Category : Science
ISBN : 9783527342679

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Aptamers for Analytical Applications by Yiyang Dong Pdf

An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.

Author : Paul Hansoo Kim
Publisher : Unknown
Page : 816 pages
File Size : 46,5 Mb
Release : 2005
Category : Nucleic acids
ISBN : UCR:31210019700598

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Design of Nucleic Acids by Paul Hansoo Kim Pdf

Author : Günter Mayer
Publisher : John Wiley & Sons
Page : 595 pages
File Size : 41,7 Mb
Release : 2011-06-17
Category : Science
ISBN : 9781119957263

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The Chemical Biology of Nucleic Acids by Günter Mayer Pdf

With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include: chemical synthesis of modified RNA expansion of the genetic alphabet in nucleic acids by creating new base pairs chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides nucleic-acid-templated chemistry chemical biology of peptide nucleic acids (PNA) the interactions of small molecules with DNA and RNA the architectural modules of folded RNAs genesis and biological applications of locked nucleic acid (LNA) small non-coding RNA in bacteria microRNA-guided gene silencing nucleic acids based therapies innate immune recognition of nucleic acid light-responsive nucleic acids for the spatiotemporal control of biological processes DNA methylation frameworks for programming RNA devices RNA as a catalyst: The Diels-Alderase-Ribozyme evolving an understanding of RNA function by in vitro approaches the chemical biology of aptamers: synthesis and applications nucleic acids as detection tools bacterial riboswitch discovery and analysis The Chemical Biology of Nucleic Acids is an essential compendium of the synthesis of nucleic acids and their biological applications for bioorganic chemists, chemical biologists, medicinal chemists, cell biologists, and molecular biologists.

Author : Satoshi Obika,Mitsuo Sekine
Publisher : Springer
Page : 284 pages
File Size : 46,8 Mb
Release : 2018-12-08
Category : Medical
ISBN : 9789811319129

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Synthesis of Therapeutic Oligonucleotides by Satoshi Obika,Mitsuo Sekine Pdf

This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 41,8 Mb
Release : 1983
Category : Electronic
ISBN : OCLC:873950560

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Enzymes of Nucleic Acid Synthesis and Modification by Anonim Pdf

Author : G Michael Blackburn,Michael J Gait,David Loakes,David M Williams
Publisher : Royal Society of Chemistry
Page : 824 pages
File Size : 44,9 Mb
Release : 2015-11-09
Category : Science
ISBN : 9781782625773

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Nucleic Acids in Chemistry and Biology by G Michael Blackburn,Michael J Gait,David Loakes,David M Williams Pdf

The structure, function and reactions of nucleic acids are central to molecular biology and are crucial for the understanding of complex biological processes involved. Revised and updated Nucleic Acids in Chemistry and Biology 3rd Edition discusses in detail, both the chemistry and biology of nucleic acids and brings RNA into parity with DNA. Written by leading experts, with extensive teaching experience, this new edition provides some updated and expanded coverage of nucleic acid chemistry, reactions and interactions with proteins and drugs. A brief history of the discovery of nucleic acids is followed by a molecularly based introduction to the structure and biological roles of DNA and RNA. Key chapters are devoted to the chemical synthesis of nucleosides and nucleotides, oligonucleotides and their analogues and to analytical techniques applied to nucleic acids. The text is supported by an extensive list of references, making it a definitive reference source. This authoritative book presents topics in an integrated manner and readable style. It is ideal for graduate and undergraduates students of chemistry and biochemistry, as well as new researchers to the field.

Author : Roland F. Beers,R. Carmichael Tilghman
Publisher : Unknown
Page : 360 pages
File Size : 46,9 Mb
Release : 1973
Category : Health & Fitness
ISBN : UCAL:B4300251

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Cellular Modification and Genetic Transformation by Exogenous Nucleic Acids by Roland F. Beers,R. Carmichael Tilghman Pdf