Author : Joseph Sambrook
Publisher : Unknown
Page : 0 pages
File Size : 44,9 Mb
Release : 2003
Category : Eukaryotic cells
ISBN : 0879695773
Molecular Cloning
Joseph Sambrook
Molecular Cloning
Molecular Cloning Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Molecular Cloning book. This book definitely worth reading, it is an incredibly well-written.
DNA Cloning: A Hands-on Approach
Seok-Yong Choi,Hyunju Ro,Hankuil Yi
Author : Seok-Yong Choi,Hyunju Ro,Hankuil Yi
Publisher : Springer
Page : 131 pages
File Size : 43,8 Mb
Release : 2019-04-17
Category : Medical
ISBN : 9789402416626
DNA Cloning: A Hands-on Approach by Seok-Yong Choi,Hyunju Ro,Hankuil Yi Pdf
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Molecular Biology
Susan J. Karcher
Author : Susan J. Karcher
Publisher : Academic Press
Page : 280 pages
File Size : 49,6 Mb
Release : 1995-11-28
Category : Science
ISBN : 0080536832
Molecular Biology by Susan J. Karcher Pdf
This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)
Gene Cloning
Julia Lodge,Peter Lund,Steve Minchin
Author : Julia Lodge,Peter Lund,Steve Minchin
Publisher : Garland Science
Page : 612 pages
File Size : 52,7 Mb
Release : 2007-01-24
Category : Science
ISBN : 9781135728922
Gene Cloning by Julia Lodge,Peter Lund,Steve Minchin Pdf
The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.
Scientific and Medical Aspects of Human Reproductive Cloning
National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Engineering, and Public Policy
Author : National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Engineering, and Public Policy
Publisher : National Academies Press
Page : 295 pages
File Size : 52,6 Mb
Release : 2002-06-17
Category : Science
ISBN : 9780309076371
Scientific and Medical Aspects of Human Reproductive Cloning by National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Engineering, and Public Policy Pdf
Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.
PCR Cloning Protocols
Bing-Yuan Chen,Harry W. Janes
Author : Bing-Yuan Chen,Harry W. Janes
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 49,9 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591770
PCR Cloning Protocols by Bing-Yuan Chen,Harry W. Janes Pdf
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
DNA Cloning and Assembly Methods
Svein Valla,Rahmi Lale
Author : Svein Valla,Rahmi Lale
Publisher : Humana Press
Page : 0 pages
File Size : 40,9 Mb
Release : 2014-01-07
Category : Medical
ISBN : 1627037632
DNA Cloning and Assembly Methods by Svein Valla,Rahmi Lale Pdf
In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
The ABCs of Gene Cloning
Dominic Wong
Author : Dominic Wong
Publisher : Springer Science & Business Media
Page : 227 pages
File Size : 43,5 Mb
Release : 2007-12-31
Category : Science
ISBN : 9780387286792
The ABCs of Gene Cloning by Dominic Wong Pdf
Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.
A Practical Guide to Molecular Cloning
Bernard V. Perbal
Author : Bernard V. Perbal
Publisher : Wiley-Interscience
Page : 582 pages
File Size : 50,7 Mb
Release : 1984
Category : Genetic engineering
ISBN : UCSD:31822002323822
A Practical Guide to Molecular Cloning by Bernard V. Perbal Pdf
Presents techniques tested at the Curie Institute and other leading labs and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. Offers detailed explanation of protocols, allowing the isolation, cloning, and expression of genes from living species. Presents up-to-date techniques on sequencing, in vitro expression of cloned gene, and use of computers for study of nucleic acids, and is the only book that shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes. Contains 235 figures and 80 tables.
Current Protocols in Molecular Biology
Anonim
Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 52,7 Mb
Release : 2024-06-18
Category : Molecular biology
ISBN : 047150338X
Current Protocols in Molecular Biology by Anonim Pdf
Protein-protein Interactions
Erica A. Golemis,Erica Golemis,Peter David Adams
Author : Erica A. Golemis,Erica Golemis,Peter David Adams
Publisher : CSHL Press
Page : 954 pages
File Size : 46,7 Mb
Release : 2005
Category : Interactions protéine-protéine - Manuels de laboratoire
ISBN : 9780879697235
Protein-protein Interactions by Erica A. Golemis,Erica Golemis,Peter David Adams Pdf
Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.
Molecular Cloning
Gregory G. Brown
Author : Gregory G. Brown
Publisher : BoD – Books on Demand
Page : 340 pages
File Size : 45,6 Mb
Release : 2011-10-12
Category : Medical
ISBN : 9789533073989
Molecular Cloning by Gregory G. Brown Pdf
The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.
DNA Cloning and Assembly
Sunil Chandran,Kevin W. George
Author : Sunil Chandran,Kevin W. George
Publisher : Humana
Page : 334 pages
File Size : 45,8 Mb
Release : 2021-09-02
Category : Medical
ISBN : 1071609106
DNA Cloning and Assembly by Sunil Chandran,Kevin W. George Pdf
This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Molecular Biology of The Cell
Bruce Alberts
Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 42,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181
Molecular Biology of The Cell by Bruce Alberts Pdf
Gene Cloning and Manipulation
Christopher Howe
Author : Christopher Howe
Publisher : Cambridge University Press
Page : 250 pages
File Size : 43,8 Mb
Release : 2007-07-12
Category : Medical
ISBN : 9781139463584
Gene Cloning and Manipulation by Christopher Howe Pdf
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
