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Morphogenesis in Plant Tissue Cultures by Woong-Young Soh,Sant Saran Bhojwani Pdf
This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.
Plant Cell and Tissue Culture by Indra K. Vasil,Trevor A. Thorpe Pdf
Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.
In the past there were many attempts to change natural foodstuffs into high-value products. Cheese, bread, wine, and beer were pro duced, traditionally using microorganisms as biological tools. Later, people influenced the natural process of evolution by artificial selection. In the 19th century, observations regarding the depen dence of growth and reproduction on the nutrient supply led to the establishment of agricultural chemistry. Simultaneously, efforts were directed at defining the correlation between special forms of morphological differentiation and related biochemical processes. New experimental systems were developed after the discovery of phytohormones and their possible use as regulators of growth and differentiation. In these systems, intact plants or only parts of them are cultivated under axenic conditions. These methods, called "in vitro techniques", were introduced to modern plant breeding. In the field of basic research, plant cell cultures were increasingly developed and the correlations between biochemical processes and visible cell variations were explored further. It should be possible to manipulate the basic laws of regulation and the respective biochemi cal processes should be regarded as being independent of morpho logical processes of plant development.
A Handbook of Plant Tissue Culture by Philip Rodney White Pdf
The history of plant tissue culture. The living materials. The laboratory. Nutrients. How cultures are started. Culture techniques. Growth measurements and their interpretation. Tissue culture and the study of problems in the pathology and general physiology of plants. Morphogenesis.
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Author : John H. Dodds,Lorin W. Roberts Publisher : International Potato Center Page : 260 pages File Size : 40,8 Mb Release : 1985-10-31 Category : Science ISBN : 0521315166
Experiments in Plant Tissue Culture by John H. Dodds,Lorin W. Roberts Pdf
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
Morphogenesis in Plants by Claude Wilson Wardlaw Pdf
Introduction; Embryology; The shoot apex; Experiments on the shoot apex; Leaf formation and related problems; Mathematical relationships; Biochemistry and morphogenesis; The genic control of morphogenesis; Evidence of genic action in morphogenesis.
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Physiology, Growth and Development of Plants in Culture by P.J. Lumsden,J.R. Nicholas,W.J. Davies Pdf
Over recent years, progress in micropropagation has not been as rapid as many expected and, even now, relatively few crops are produced commercially. One reason for this is that the biology of material growing in vitro has been insufficiently understood for modifications to standard methods to be made based on sound physiological principles. However, during the past decade, tissue culture companies and others have invested considerable effort to reduce the empirical nature of the production process. The idea of the conference `Physiology, Growth and Development of Plants and Cells in Culture' (Lancaster, 1992) was to introduce specialists in different areas of plant physiology to micropropagators, with the express aims of disseminating as wide a range of information to as large a number of participants as possible, and beginning new discussions on the constraints and potentials affecting the development of in vitro plant production methods. This book is based on presentations from the conference and has been divided into two main sections, dealing with either aspects of the in vitro environment -- light, nutrients, water, gas -- or with applied aspects of the culture process -- morphogenesis, acclimation, rejuvenation, contamination.
Randolph R. Henke,Karen W. Hughes,Milton J. Constantin,Alexander Hollaender,Claire M. Wilson
Author : Randolph R. Henke,Karen W. Hughes,Milton J. Constantin,Alexander Hollaender,Claire M. Wilson Publisher : Springer Science & Business Media Page : 390 pages File Size : 53,6 Mb Release : 2013-11-11 Category : Technology & Engineering ISBN : 9781489903785
Tissue Culture in Forestry and Agriculture by Randolph R. Henke,Karen W. Hughes,Milton J. Constantin,Alexander Hollaender,Claire M. Wilson Pdf
This symposium is the third in a series featuring the propaga tion of higher plants through tissue culture. The first of these symposia, entitled "A Bridge Between Research and Application," was held at the University in 1978 and was published by the Technical Information Center, Department of Energy. The second symposium, on "Emerging Technologies and Strategies," was held in 1980 and pub lished as a special issue of Environmental and Experimental Botany. One of the aims of these symposia was to examine the current state of-the-art in tissue culture technology and to relate this state of technology to practical, applied, and commercial interests. Thus, the third of this series on development and variation focused on embryogenesis in culture: how to recognize it, factors which affect embryogenesis, use of embryogenic systems, etc.; and variability from culture. A special session on woody species again emphasized somatic embryogenesis as a means of rapid propagation. This volume emphasizes tissue culture of forest trees. All of these areas, we feel, are breakthrough areas in which significant progress is expected in the next few years.
Thin Cell Layer Culture System: Regeneration and Transformation Applications by Duong Tan Nhut,Kiem Tran Thanh Van,B. Van Le,Trevor A. Thorpe Pdf
Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry. The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants. This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.
Plant Tissue Culture and Its Agricultural Applications by Lyndsey A. Withers,P. G. Alderson Pdf
Plant Tissue Culture and Its Agricultural Applications presents the proceedings of the 41st University of Nottingham Easter School in Agricultural Science held in England. The sessions covered in this volume reflect the revolution of tissue culture and its role in the propagation of elite plant material and the development of improved genotypes. This book is organized into four main sections. The first section chronicles the revolution of the plant tissue culture. This includes papers on clonal propagation, morphogenesis, germplasm storage, plant health, and genetic improvement. The core of this volume is covered by the introductory and the final chapters which interrelate the different subjects areas covered by the proceedings and provide a realistic assessment of future research required for the plant tissue culture revolution to come to fruition. This book will be useful to readers interested in understanding the history, evolution, and future of plant tissue culture and its applications in the agricultural sector.
