Neuronal Cell Culture

Author : Shohreh Amini,Martyn K. White
Publisher : Humana
Page : 205 pages
File Size : 46,8 Mb
Release : 2022-06-09
Category : Medical
ISBN : 1071614398

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Neuronal Cell Culture by Shohreh Amini,Martyn K. White Pdf

This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.

Author : Jane Bottenstein
Publisher : Springer Science & Business Media
Page : 392 pages
File Size : 50,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461324737

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Cell Culture in the Neurosciences by Jane Bottenstein Pdf

A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.

Author : Shohreh Amini,Martyn K. White
Publisher : Unknown
Page : 165 pages
File Size : 42,5 Mb
Release : 2013
Category : Cell culture
ISBN : 1627036407

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Neuronal Cell Culture by Shohreh Amini,Martyn K. White Pdf

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

Author : Gary Banker,Kimberly Goslin
Publisher : MIT Press
Page : 706 pages
File Size : 55,5 Mb
Release : 1998
Category : Anatomy
ISBN : 0262024381

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Culturing Nerve Cells by Gary Banker,Kimberly Goslin Pdf

A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

Author : Sergey Fedoroff,Arleen Richardson
Publisher : Springer Science & Business Media
Page : 366 pages
File Size : 41,5 Mb
Release : 2008-06-29
Category : Medical
ISBN : 9781592592074

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Protocols for Neural Cell Culture by Sergey Fedoroff,Arleen Richardson Pdf

The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.

Author : L. W. Haynes
Publisher : Unknown
Page : 780 pages
File Size : 47,8 Mb
Release : 1999-02-22
Category : Medical
ISBN : UOM:39015042761794

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The Neuron in Tissue Culture by L. W. Haynes Pdf

This volume in the Handbook Series contains 73 contributions from experts in the range of fields to which neuronal tissue culture may be applied. Twenty-two keynote chapters contain methods, well-illustrated with data from the contributing authors? laboratones, which exemplify ?landmark? applications of the technique. Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum. The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book. The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons. It is essential reading for all those with research interests in neuroscience, including developmental biologists, neurologists, nerve cell physiologists and neuropharmaoologists.

Author : Peter Riederer,Heinz Reichmann,Moussa B. H. Youdim,Manfred Gerlach
Publisher : Springer Science & Business Media
Page : 484 pages
File Size : 45,9 Mb
Release : 2006-11-23
Category : Medical
ISBN : 9783211452950

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Parkinson's Disease and Related Disorders by Peter Riederer,Heinz Reichmann,Moussa B. H. Youdim,Manfred Gerlach Pdf

This book gives a comprehensive overview on current clinical and basic research issues related to Parkinson’s disease and its related disorders. The book is the result of the 16th International Congress of Parkinson’s Disease and Related disorders congress 2005. Its contents are suitable for neurologists, psychiatrists, neurosurgeons, basic researchers, geneticians and patients as well as their relatives.

Author : David Andrew Sirbasku,Gordon Sato
Publisher : Unknown
Page : 288 pages
File Size : 52,6 Mb
Release : 1984
Category : Cell culture
ISBN : UCAL:B4344417

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Methods for Serum-free Culture of Neuronal and Lymphoid Cells by David Andrew Sirbasku,Gordon Sato Pdf

Author : Manfred Gerlach,Jürgen Deckert,Kay Double,Eleni Koutsilieri
Publisher : Springer Science & Business Media
Page : 326 pages
File Size : 41,9 Mb
Release : 2007-12-28
Category : Medical
ISBN : 9783211735749

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Neuropsychiatric Disorders by Manfred Gerlach,Jürgen Deckert,Kay Double,Eleni Koutsilieri Pdf

This multidisciplinary book includes current research papers and reviews in the areas of basic neuroscience, neural mechanisms underlying neurodegenerative disorders. It further includes new approaches for neuroprotective treatments, clinical, neurobiological and treatment aspects of psychiatric disorders. The book was conceived as a celebration of the professional life and work of Peter Riederer to mark the occasion of his retirement.

Author : John N. Wood
Publisher : Oxford University Press, USA
Page : 296 pages
File Size : 51,7 Mb
Release : 1992
Category : Cell lines
ISBN : PSU:000020456429

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Neuronal Cell Lines by John N. Wood Pdf

Author : Steven E. Pfeiffer
Publisher : Springer
Page : 256 pages
File Size : 40,6 Mb
Release : 1982-08-30
Category : Medical
ISBN : UOM:39015010062233

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Neuroscience Approached Thru Cell Culture by Steven E. Pfeiffer Pdf

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 51,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Nicole Le Douarin,Chaya Kalcheim
Publisher : Cambridge University Press
Page : 494 pages
File Size : 46,8 Mb
Release : 1999-11-28
Category : Medical
ISBN : 0521620104

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The Neural Crest by Nicole Le Douarin,Chaya Kalcheim Pdf

This 1999 edition of The Neural Crest contains comprehensive information about the neural crest, a structure unique to the vertebrate embryo, which has only a transient existence in early embryonic life. The ontogeny of the neural crest embodies the most important issues in developmental biology, as the neural crest is considered to have played a crucial role in evolution of the vertebrate phylum. Data that analyse neural crest ontogeny in murine and zebrafish embryos have been included in this revision. This revised edition also takes advantage of recent advances in our understanding of markers of neural crest cell subpopulations, and a full chapter is now devoted to cell lineage analysis. The major research breakthrough since the first edition has been the introduction of molecular biology to neural crest research, enabling an elucidation of many molecular mechanisms of neural crest development. This book is essential reading for students and researchers in developmental biology, cell biology, and neuroscience.

Author : Michael Aschner,Lucio Costa
Publisher : Humana
Page : 302 pages
File Size : 40,5 Mb
Release : 2020-05-10
Category : Medical
ISBN : 1493992309

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Cell Culture Techniques by Michael Aschner,Lucio Costa Pdf

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

Author : Michela Chiappalone,Valentina Pasquale,Monica Frega
Publisher : Springer
Page : 387 pages
File Size : 47,6 Mb
Release : 2019-05-09
Category : Medical
ISBN : 9783030111359

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In Vitro Neuronal Networks by Michela Chiappalone,Valentina Pasquale,Monica Frega Pdf

This book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies.