New Methods For Culturing Cells From Nervous Tissues
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New Methods for Culturing Cells from Nervous Tissues by Philippe Poindron,Pascale Piguet,Dr. Eckart Förster Pdf
"Presenting expert guidelines to prepare, establish and maintain neural cell and tissue cultures, this book will be a valuable tool for all scientists and technicians interested in basic and applied research in neurobiology, neurology and neuropharmacology."--BOOK JACKET.
Author : Gordon Sato Publisher : Springer Science & Business Media Page : 298 pages File Size : 42,5 Mb Release : 2013-06-29 Category : Medical ISBN : 9781468429046
Tissue Culture of the Nervous System by Gordon Sato Pdf
The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad vantages of these cultures.
Protocols for Neural Cell Culture by Sergey Fedoroff,Arleen Richardson Pdf
The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.
Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research
Author : Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research Publisher : National Academies Press Page : 112 pages File Size : 41,7 Mb Release : 2002-01-25 Category : Science ISBN : 9780309170420
Stem Cells and the Future of Regenerative Medicine by Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research Pdf
Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.
Neuronal Cell Culture by Shohreh Amini,Martyn K. White Pdf
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
A Dissection and Tissue Culture Manual of the Nervous System by Abraham Shahar Pdf
At last … a collection of practical protocols for explanting and manipulating neuronal and glial cells. A Dissection and Tissue Culture Manual of the Nervous System Abraham Shahar, Jean de Vellis, Antonia Vernadakis, and Bernard Haber, Editors Among research laboratories involved with neuronal and glial cell cultures and their applications, there is a growing demand for a hand-book describing dissection procedures, culture preparation techniques, and the in vitro manipulation of neural cells and tissues for specific analytical purposes. A Dissection and Tissue Culture Manual of the Nervous System offers a diverse collection of methods that have been developed by and are used routinely within specialized neurobiological laboratories. Written in an easy-to-follow style, the procedures described in this unique guide are designed by experts to be applied by those with limited experience in the field. Organized into ten comprehensive sections, ninety concise contributions from leading laboratories worldwide put forth practical, stepwise protocols for neural cell manipulation and experimentation. Methods encompass: an illustrated outline of techniques for the dissection of brain areas in the fetus and the neonate the dissection of selected specialized structures, such as the ciliary ganglion organotypic. explant culture of nervous tissue dissociated culture of astrocytes, oligodendrocyte, neurons, and Schwann cells reaggregation culture of dissociated cells. Sections devoted to various tissue processing methods and experimental applications of cultured material present histochemical, autoradio-graphic, and immunocytochemical staining and visualization techniques. In situ hybridization methods, as well as preparative procedures for electron microscopy and biochemical and physiological assays, are discussed with an emphasis on methods tailored for the neurobiologist. Alternative techniques for the cultivation of the same organ or cell type from diverse animal species are juxtaposed with a varied selection of methodology and instrumentation, and complemented by key literature citations for further reading, to enable the investigator to chose the appropriate approach for a specific neurobiological application. Presented in a comb-bound format for convenient use on the laboratory bench, A Dissection and Tissue Culture Manual of the Nearvous System will be an essential research companion to graduate students, post-doctoral fellows and other laborabory investigators in cell and developmental neurobiology, neuroanatomy, neurophysiology, neuropharmacology, and biochemistry.
National Academy of Sciences,Institute of Medicine,Sandra Ackerman
Author : National Academy of Sciences,Institute of Medicine,Sandra Ackerman Publisher : National Academies Press Page : 195 pages File Size : 40,5 Mb Release : 1992-01-01 Category : Medical ISBN : 9780309045292
Discovering the Brain by National Academy of Sciences,Institute of Medicine,Sandra Ackerman Pdf
The brain ... There is no other part of the human anatomy that is so intriguing. How does it develop and function and why does it sometimes, tragically, degenerate? The answers are complex. In Discovering the Brain, science writer Sandra Ackerman cuts through the complexity to bring this vital topic to the public. The 1990s were declared the "Decade of the Brain" by former President Bush, and the neuroscience community responded with a host of new investigations and conferences. Discovering the Brain is based on the Institute of Medicine conference, Decade of the Brain: Frontiers in Neuroscience and Brain Research. Discovering the Brain is a "field guide" to the brainâ€"an easy-to-read discussion of the brain's physical structure and where functions such as language and music appreciation lie. Ackerman examines: How electrical and chemical signals are conveyed in the brain. The mechanisms by which we see, hear, think, and pay attentionâ€"and how a "gut feeling" actually originates in the brain. Learning and memory retention, including parallels to computer memory and what they might tell us about our own mental capacity. Development of the brain throughout the life span, with a look at the aging brain. Ackerman provides an enlightening chapter on the connection between the brain's physical condition and various mental disorders and notes what progress can realistically be made toward the prevention and treatment of stroke and other ailments. Finally, she explores the potential for major advances during the "Decade of the Brain," with a look at medical imaging techniquesâ€"what various technologies can and cannot tell usâ€"and how the public and private sectors can contribute to continued advances in neuroscience. This highly readable volume will provide the public and policymakersâ€"and many scientists as wellâ€"with a helpful guide to understanding the many discoveries that are sure to be announced throughout the "Decade of the Brain."
Neuronal Cell Culture by Shohreh Amini,Martyn K. White Pdf
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Recovery of Motor Function Following Spinal Cord Injury by Heidi Fuller,Monte Gates Pdf
Restoration of motor function following spinal cord injury is a complex and challenging task. By reviewing emerging cellular, pharmacological, rehabilitative, as well as surgical approaches, this book seeks to highlight promising therapeutic strategies for the repair and regeneration of motor circuitry. The multidisciplinary nature of these approaches illustrates various routes to bridging the gap between the bench and the bedside and to identify the challenges that must be overcome in order to bring about a viable therapeutic strategy for spinal cord injury patients.
Guide to Research Techniques in Neuroscience by Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer Pdf
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
Protocols for Neural Cell Culture by Sergey Fedoroff,Arleen Richardson Pdf
Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 new chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as are essential procedures for hippocampal and microglial slice cultures. Protocols for Neural Cell Culture: Third Edition is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture and its disorders.
Introduction to Cell and Tissue Culture by Jennie P. Mather,Penelope E. Roberts Pdf
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.