Nucleic Acid Transfection

Author : Wolfgang Bielke,Christoph Erbacher
Publisher : Springer Science & Business Media
Page : 316 pages
File Size : 41,5 Mb
Release : 2010-10-21
Category : Science
ISBN : 9783642164293

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Nucleic Acid Transfection by Wolfgang Bielke,Christoph Erbacher Pdf

Gene Delivery into Mammalian Cells: An Overview on Existing Approaches Employed In Vitro and In Vivo, by Peter Hahn and Elizabeth Scanlan * Strategies for the Preparation of Synthetic Transfection Vectors, by Asier Unciti-Broceta, Matthew N. Bacon, and Mark Bradley * Cationic Lipids: Molecular Structure/Transfection Activity Relationships and Interactions with Biomembranes, by Rumiana Koynova and Boris Tenchov * Hyperbranched Polyamines for Transfection, by Wiebke Fischer, Marcelo Calderon, and Rainer Haag * Carbohydrate Polymers for Nonviral Nucleic Acid Delivery, by Antons Sizovs, Patrick M. McLendon, Sathya Srinivasachari, and Theresa M. Reineke * Cationic Liposome–Nucleic Acid Complexes for Gene Delivery and Silencing: Pathways and Mechanisms for Plasmid DNA and siRNA, by Kai K. Ewert, Alexandra Zidovska, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans, Christopher S. McAllister, Charles E. Samuel, and Cyrus R. Safinya * Chemically Programmed Polymers for Targeted DNA and siRNA Transfection, by Eveline Edith Salcher and Ernst Wagner * Photochemical Internalization: A New Tool for Gene and Oligonucleotide Delivery, by Kristian Berg, Maria Berstad, Lina Prasmickaite, Anette Weyergang, Pål K. Selbo, Ida Hedfors, and Anders Høgset * Visualizing Uptake and Intracellular Trafficking of Gene Carriers by Single-Particle Tracking, by N. Ruthardt and C. Bräuchle

Author : G.R. Dubes
Publisher : Springer-Verlag
Page : 77 pages
File Size : 52,5 Mb
Release : 2013-11-21
Category : Science
ISBN : 9783034857734

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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review by G.R. Dubes Pdf

Author : Dan Luo,W. Mark Saltzman
Publisher : Springer Science & Business Media
Page : 160 pages
File Size : 50,7 Mb
Release : 2003-09-30
Category : Science
ISBN : 0306477017

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Synthetic DNA Delivery Systems by Dan Luo,W. Mark Saltzman Pdf

DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Author : George R. Dubes
Publisher : Unknown
Page : 0 pages
File Size : 48,7 Mb
Release : 1971
Category : Electronic
ISBN : OCLC:251349567

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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses by George R. Dubes Pdf

Author : Brian Ceresa
Publisher : BoD – Books on Demand
Page : 470 pages
File Size : 53,8 Mb
Release : 2012-07-06
Category : Science
ISBN : 9789535106623

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Molecular Regulation of Endocytosis by Brian Ceresa Pdf

Molecular Regulation of Endocytosis is a compilation of scientific "short stories" about the entry of external substances into cells. As one can see from the chapters, endocytosis regulates diverse processes such as homeostasis of the cell, signal transduction, entry of pathogens and viruses. In addition to the experimental techniques embedded in each chapter, entire chapters are dedicated to experimental approaches that will be useful to all scientists and their model systems. For those more clinically oriented, the final chapters look to the future and ways of utilizing endocytic pathways for therapeutic purposes.

Author : Edward J. Murray
Publisher : Springer Science & Business Media
Page : 417 pages
File Size : 43,9 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592594948

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Gene Transfer and Expression Protocols by Edward J. Murray Pdf

Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .

Author : Ram I. Mahato,Sung Wan Kim
Publisher : CRC Press
Page : 521 pages
File Size : 52,9 Mb
Release : 2002-06-27
Category : Medical
ISBN : 9780203300961

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Pharmaceutical Perspectives of Nucleic Acid-Based Therapy by Ram I. Mahato,Sung Wan Kim Pdf

Emphasizing its uses in cancer and cardiovascular and autoimmune diseases, Pharmaceutical Perspectives of Nucleic Acid-Based Therapy presents a comprehensive account of gene therapy, from development in the laboratory to clinical applications. Internationally acclaimed scientists discuss the potential use of lipids, peptides and polymers for the in

Author : Yue Yanan
Publisher : Springer Science & Business Media
Page : 108 pages
File Size : 51,8 Mb
Release : 2013-05-13
Category : Technology & Engineering
ISBN : 9783319003368

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How Free Cationic Polymer Chains Promote Gene Transfection by Yue Yanan Pdf

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

Author : Volker A. Erdmann,Jan Barciszewski
Publisher : Springer Science & Business Media
Page : 650 pages
File Size : 52,5 Mb
Release : 2012-06-13
Category : Science
ISBN : 9783642274268

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From Nucleic Acids Sequences to Molecular Medicine by Volker A. Erdmann,Jan Barciszewski Pdf

Despite a half century of structural, biophysical and biochemical investigations of ribonucleic acids, they are still mysterious. RNAs stand at fertile crossroads of disciplines, integrating concepts from genomics, proteomics, dynamics as well as biochemistry and molecular biology. From 20 years it is clear, that genetic regulation of eukaryotic organisms has been misunderstood for the last years that the expression of genetic information is effected only by proteins. Basic understanding of nucleic acids has enhanced our foundation to probe novel biological functions. This is especially evident for RNA molecules whose functionality, maturation, and regulation require formation of correct secondary structure through encoded base-pairing interactions.

Author : Martin Schleef
Publisher : John Wiley & Sons
Page : 373 pages
File Size : 50,7 Mb
Release : 2013-02-14
Category : Science
ISBN : 9783527670444

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Minicircle and Miniplasmid DNA Vectors by Martin Schleef Pdf

This first title on the topic provides complete coverage, including the molecular basis, production and possible biomedical applications. Written by the most prominent academic researchers in the field as well as by researchers at one of the world's leading companies in industrial production of minicircle DNA, this practical book is aimed at everyone who is directly or indirectly involved in the development of gene therapies.

Author : Stephan Sudowe,Angelika B. Reske-Kunz
Publisher : Humana Press
Page : 432 pages
File Size : 45,9 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493960687

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Biolistic DNA Delivery by Stephan Sudowe,Angelika B. Reske-Kunz Pdf

Another key addition to Springer s Methods in Molecular Biology series, this volume includes methods for direct biolistic transfection of DNA using ballistic devices such as a gene gun. Lab-ready protocols and expert troubleshooting guarantee reproducibility."

Author : Chad A. Mirkin
Publisher : CRC Press
Page : 1806 pages
File Size : 48,7 Mb
Release : 2020-09-01
Category : Medical
ISBN : 9780429578069

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Spherical Nucleic Acids by Chad A. Mirkin Pdf

Spherical nucleic acids (SNAs) comprise a nanoparticle core and a densely packed and highly oriented nucleic acid shell, typically DNA or RNA. They have novel architecture-dependent properties that distinguish them from all other forms of nucleic acids and make them useful in materials synthesis, catalysis, diagnostics, therapeutics, and optics/plasmonics. This book covers over two decades of Dr. Mirkin’s research on SNAs and their anisotropic analogues, including synthesis and fundamental properties, and applications in colloidal crystallization, adaptive matter, and nanomedicine, spanning extra- and intracellular diagnostics, gene regulation, and immunomodulation. It is a reprint volume that compiles 101 key papers from high-impact journals in this research area published by the Mirkin Group at Northwestern University, Illinois, USA, within the International Institute for Nanotechnology, and collaborators. Volume 1 provides an overview and a historical framework of engineering matter from DNA-modified constructs and discusses the enabling features of nucleic acid–functionalized nanomaterials. Volume 2 covers design rules for colloidal crystallization, building blocks for crystal engineering, and DNA and RNA as programmable bonds. Volume 3 discusses colloidal crystallization processes and routes to hierarchical assembly, dynamic nanoparticle superlattices, surface-based and template-confined colloidal crystallization, optics and plasmonics with nanoparticle superlattices, and postsynthetic modification and catalysis with nanoparticle superlattices. Volume 4 covers diagnostic modalities, and intracellular therapeutic and diagnostic schemes based upon nucleic acid–functionalized nanomaterials.

Author : Cornel Mulhardt
Publisher : Elsevier
Page : 272 pages
File Size : 51,8 Mb
Release : 2010-07-19
Category : Science
ISBN : 0080467660

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Molecular Biology and Genomics by Cornel Mulhardt Pdf

Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure, function, and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner, addressing the assets and drawbacks as well as critical points. It also provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. Shows how to avoid experimental dead ends and develops an instinct for the right experiment at the right time Includes a handy Career Guide for researchers in the field Contains more than 100 extensive figures and tables

Author : Stefan Bauer,Gunther Hartmann
Publisher : Springer Science & Business Media
Page : 243 pages
File Size : 53,7 Mb
Release : 2007-12-11
Category : Medical
ISBN : 9783540721673

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Toll-Like Receptors (TLRs) and Innate Immunity by Stefan Bauer,Gunther Hartmann Pdf

Overall recent research on TLRs has led to tremendous increase in our understanding of early steps in pathogen recognition and will presumably lead to potent TLR targeting therapeutics in the future. This book reviews and highlights our recent understanding on the function and ligands of TLRs as well as their role in autoimmunity, dendritic cell activation and target structures for therapeutic intervention.

Author : Tzvi Tzfira,Vitaly Citovsky
Publisher : Springer Science & Business Media
Page : 239 pages
File Size : 51,6 Mb
Release : 2007-02-25
Category : Science
ISBN : 9780387277479

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Nuclear Import and Export in Plants and Animals by Tzvi Tzfira,Vitaly Citovsky Pdf

Nuclear Import and Export in Plants and Animals provides insight into the remarkable mechanisms of nuclear import and export. This book covers a range of topics from the nuclear pore structure, to nuclear import and export of macromolecules in plant and animal cells. In addition, the book covers the special cases of nuclear import of Agrobacterium T-DNA during plant genetic transformation, nuclear import and export of animal viruses, and nuclear intake of foreign DNA. A chapter on research methods to study nuclear transport concludes the book.