Pcr Protocols For Diagnosis Of Human And Animal Virus Diseases

Author : Yechiel Becker
Publisher : Springer
Page : 128 pages
File Size : 54,5 Mb
Release : 1995-01-01
Category : Medical
ISBN : 038758899X

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Pcr by Yechiel Becker Pdf

Author : Jonathan P. Clewley
Publisher : CRC Press
Page : 244 pages
File Size : 47,9 Mb
Release : 1994-10-27
Category : Science
ISBN : 0849348331

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The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis by Jonathan P. Clewley Pdf

The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.

Author : Wang-Shick Ryu
Publisher : Academic Press
Page : 440 pages
File Size : 42,9 Mb
Release : 2016-03-30
Category : Science
ISBN : 9780128009994

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Molecular Virology of Human Pathogenic Viruses by Wang-Shick Ryu Pdf

Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author’s more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. Presents viruses within their family structure Contains recommended journal articles with perspectives to put primary literature in context Includes integrated recommended reading references within each chapter Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor’s manual, study guide, and test bank

Author : Rajib Deb,Ajay Kumar Yadav,Swaraj Rajkhowa,Yashpal Singh Malik
Publisher : Unknown
Page : 381 pages
File Size : 47,9 Mb
Release : 2022
Category : Swine
ISBN : 1071620436

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Protocols for the Diagnosis of Pig Viral Diseases by Rajib Deb,Ajay Kumar Yadav,Swaraj Rajkhowa,Yashpal Singh Malik Pdf

This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields. Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

Author : Yechiel Becker,Gholamreza Darai
Publisher : Springer
Page : 0 pages
File Size : 53,5 Mb
Release : 2012-02-09
Category : Medical
ISBN : 3642847684

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology by Yechiel Becker,Gholamreza Darai Pdf

The basis for the effective treatment and cure of a patient is the rapid diagnosis of the disease and its causative agent, which is based on the analysis of the clinical symptoms coupled with laboratory tests. Although rapid advance ments have been made in the laboratory diagnosis of virus diseases, the neces sary isolation of the causative virus from the clinical specimens is a relatively long procedure. Viruses which integrate into the cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses which exist in the clinical material in low concentrations are even more formidable to identify. Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (1985) led to a revolution in virus diagnosis. The peR technique was rapidly applied to the diagnosis of viruses in clinical material. Volume 1 of Frontiers of Virology provides new information on the advan tages of the use of the peR for the diagnosis of many human disease-causing viruses, as well as on some problems with its use.

Author : Institute of Medicine,Board on Global Health,Forum on Microbial Threats
Publisher : National Academies Press
Page : 284 pages
File Size : 43,7 Mb
Release : 2007-11-11
Category : Medical
ISBN : 9780309111140

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Global Infectious Disease Surveillance and Detection by Institute of Medicine,Board on Global Health,Forum on Microbial Threats Pdf

Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.

Author : Yechiel Becker,Gholamreza Darai
Publisher : Springer
Page : 622 pages
File Size : 53,8 Mb
Release : 1995-09-26
Category : Medical
ISBN : UOM:39015040742853

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PCR: Protocols for Diagnosis of Human and Animal Virus Diseases by Yechiel Becker,Gholamreza Darai Pdf

Today the diagnosis of human and animal pathogenic viruses would be unthinkable without the polymerase chain reaction (PCR). The short-styled protocols in this volume elucidate this important technique and its application in the laboratory, including a discussion of recent advances in PCR technology. The enclosed diskette enables the user to work with interesting sequences on his own computer.

Author : Susanne Modrow,Dietrich Falke,Uwe Truyen,Hermann Schätzl
Publisher : Springer
Page : 0 pages
File Size : 47,9 Mb
Release : 2013-09-18
Category : Science
ISBN : 3642207170

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Molecular Virology by Susanne Modrow,Dietrich Falke,Uwe Truyen,Hermann Schätzl Pdf

The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.

Author : Derek J. Chadwick,Jamie A. Goode
Publisher : John Wiley & Sons
Page : 338 pages
File Size : 46,8 Mb
Release : 2001-06-29
Category : Health & Fitness
ISBN : 0471496634

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Gastroenteritis Viruses by Derek J. Chadwick,Jamie A. Goode Pdf

Ein modernes Thema - vor allem angesichts der Entwicklung eines Rotavirus-Impfstoffs und der Identifizierung zahlreicher neuer Gastroenteritis-Viren. Beschrieben werden Struktur und Molekularbiologie der SSRVs wie Caliciviren und Astroviren sowie Aspekte der Pathologie der Infektion. Der interdisziplinäre Ansatz faßt Ergebnisse aus der Grundlagen- und der klinischen Forschung zusammen.

Author : H. A. Harfi,H. Nazer,William Oh,F. B. Stapleton,R. J. Whitley
Publisher : Springer Science & Business Media
Page : 4213 pages
File Size : 40,9 Mb
Release : 2012-01-10
Category : Medical
ISBN : 9783642022029

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Textbook of Clinical Pediatrics by H. A. Harfi,H. Nazer,William Oh,F. B. Stapleton,R. J. Whitley Pdf

The new edition of this classic reference offers a problem-based approach to pediatric diseases. It encompasses almost all pediatric subspecialties and covers every pediatric disease and organ system. It includes case studies and over 750 lavish illustrations.

Author : Institute of Medicine,Board on Global Health,Forum on Emerging Infections
Publisher : National Academies Press
Page : 176 pages
File Size : 45,8 Mb
Release : 2002-04-09
Category : Medical
ISBN : 9780309169738

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The Emergence of Zoonotic Diseases by Institute of Medicine,Board on Global Health,Forum on Emerging Infections Pdf

Zoonotic diseases represent one of the leading causes of illness and death from infectious disease. Defined by the World Health Organization, zoonoses are "those diseases and infections that are naturally transmitted between vertebrate animals and man with or without an arthropod intermediate." Worldwide, zoonotic diseases have a negative impact on commerce, travel, and economies. In most developing countries, zoonotic diseases are among those diseases that contribute significantly to an already overly burdened public health system. In industrialized nations, zoonotic diseases are of particular concern for at-risk groups such as the elderly, children, childbearing women, and immunocompromised individuals. The Emergence of Zoonotic Diseases: Understanding the Impact on Animal and Human Health, covers a range of topics, which include: an evaluation of the relative importance of zoonotic diseases against the overall backdrop of emerging infections; research findings related to the current state of our understanding of zoonotic diseases; surveillance and response strategies to detect, prevent, and mitigate the impact of zoonotic diseases on human health; and information about ongoing programs and actions being taken to identify the most important needs in this vital area.

Author : Gerrit J. Viljoen,Louis H. Nel,John R. Crowther
Publisher : Springer Science & Business Media
Page : 342 pages
File Size : 43,8 Mb
Release : 2005-07-19
Category : Medical
ISBN : 1402034032

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Molecular Diagnostic PCR Handbook by Gerrit J. Viljoen,Louis H. Nel,John R. Crowther Pdf

PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.

Author : Martine Boulianne,Catherine M. Logue,Larry R. McDougald,Venugopal Nair,David L. Suarez
Publisher : John Wiley & Sons
Page : 1504 pages
File Size : 48,5 Mb
Release : 2019-11-19
Category : Medical
ISBN : 9781119371151

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Diseases of Poultry, 2 Volume Set by Martine Boulianne,Catherine M. Logue,Larry R. McDougald,Venugopal Nair,David L. Suarez Pdf

The most complete and definitive reference to all aspects of poultry diseases, Diseases of Poultry, Fourteenth Edition has been fully revised and updated to offer a comprehensive survey of current knowledge. Updates the definitive reference of poultry health and disease Provides more clinically relevant information on management of specific diseases, contributed by clinical poultry veterinarians Offers information on disease control in organic and antibiotic-free production Presents more concise, streamlined chapters for ease of use Incorporates advances in the field, from new diagnostic tools and information to changes brought about by the increasing globalization and the re-emergence of zoonotic pathogens

Author : J.R. Glisson,L.R. McDougald,L.K. Nolan,D.L. Suarez,V.L. Nair
Publisher : John Wiley & Sons
Page : 3220 pages
File Size : 50,8 Mb
Release : 2013-07-16
Category : Medical
ISBN : 9781118719732

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Diseases of Poultry by J.R. Glisson,L.R. McDougald,L.K. Nolan,D.L. Suarez,V.L. Nair Pdf

Diseases of Poultry is the most comprehensive reference for all aspects of poultry health and diseases, including pathogenesis, diagnostics, epidemiology, and control methods. Published in partnership with the American Association of Avian Pathologists, the Thirteenth Edition remains the international definitive reference, adding newer diagnostic methods and a new chapter on the emerging importance of zoonotic infections for poultry pathogens. Other updates include new high-quality photographs, additional discussion of conceptual operational biosecurity and disease control in organic production systems, and a greater emphasis throughout on the differences in disease incidence and treatments for the United States and other areas around the globe. Organized logically by disease type, the book offers detailed coverage of the history, etiology, pathobiology, diagnosis, and intervention strategies, as well as the economic and public health significance, for an exhaustive list of common and uncommon diseases. Diseases of Poultry, 13th Edition is an essential purchase for poultry veterinarians, veterinary diagnosticians, poultry scientists, students specializing in poultry health, and government officials who deal with poultry health in regulatory climate.

Author : Yashpal Singh Malik,Debmalya Barh,Vasco Azevedo,S.M. Paul Khurana
Publisher : Academic Press
Page : 564 pages
File Size : 40,5 Mb
Release : 2019-09-14
Category : Science
ISBN : 9780128163535

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Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries by Yashpal Singh Malik,Debmalya Barh,Vasco Azevedo,S.M. Paul Khurana Pdf

Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries is a comprehensive reference for animal biotechnologists, veterinary clinicians, fishery scientists, and anyone who needs to understand the latest advances in the field of next generation sequencing and genomic editing in animals and fish. This essential reference provides information on genomics and the advanced technologies used to enhance the production and management of farm and pet animals, commercial and non-commercial birds, and aquatic animals used for food and research purposes. This resource will help the animal biotechnology research community understand the latest knowledge and trends in this field. Presents biological applications of cattle, poultry, marine and animal pathogen genomics Discusses the relevance of biomarkers to improve farm animals and fishery Includes recent approaches in cloning and transgenic cattle, poultry and fish production