Diagnosis Of Human Viruses By Polymerase Chain Reaction Technology

Author : Jonathan P. Clewley
Publisher : CRC Press
Page : 244 pages
File Size : 49,6 Mb
Release : 1994-10-27
Category : Science
ISBN : 0849348331

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The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis by Jonathan P. Clewley Pdf

The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.

Author : Yechiel Becker,Gholamreza Darai
Publisher : Springer Science & Business Media
Page : 428 pages
File Size : 49,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642847660

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology by Yechiel Becker,Gholamreza Darai Pdf

The basis for the effective treatment and cure of a patient is the rapid diagnosis of the disease and its causative agent, which is based on the analysis of the clinical symptoms coupled with laboratory tests. Although rapid advance ments have been made in the laboratory diagnosis of virus diseases, the neces sary isolation of the causative virus from the clinical specimens is a relatively long procedure. Viruses which integrate into the cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses which exist in the clinical material in low concentrations are even more formidable to identify. Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (1985) led to a revolution in virus diagnosis. The peR technique was rapidly applied to the diagnosis of viruses in clinical material. Volume 1 of Frontiers of Virology provides new information on the advan tages of the use of the peR for the diagnosis of many human disease-causing viruses, as well as on some problems with its use.

Author : Danny L. Wiedbrauk,Daniel H. Farkas
Publisher : Elsevier
Page : 386 pages
File Size : 41,7 Mb
Release : 1995-02-08
Category : Science
ISBN : 0080536891

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Molecular Methods for Virus Detection by Danny L. Wiedbrauk,Daniel H. Farkas Pdf

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables

Author : Wang-Shick Ryu
Publisher : Academic Press
Page : 440 pages
File Size : 51,9 Mb
Release : 2016-03-30
Category : Science
ISBN : 9780128009994

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Molecular Virology of Human Pathogenic Viruses by Wang-Shick Ryu Pdf

Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author’s more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. Presents viruses within their family structure Contains recommended journal articles with perspectives to put primary literature in context Includes integrated recommended reading references within each chapter Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor’s manual, study guide, and test bank

Author : Yechiel Becker,Gholamreza Darai
Publisher : Springer
Page : 622 pages
File Size : 49,5 Mb
Release : 1995-09-26
Category : Medical
ISBN : UOM:39015040742853

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PCR: Protocols for Diagnosis of Human and Animal Virus Diseases by Yechiel Becker,Gholamreza Darai Pdf

Today the diagnosis of human and animal pathogenic viruses would be unthinkable without the polymerase chain reaction (PCR). The short-styled protocols in this volume elucidate this important technique and its application in the laboratory, including a discussion of recent advances in PCR technology. The enclosed diskette enables the user to work with interesting sequences on his own computer.

Author : Keith R Jerome
Publisher : CRC Press
Page : 512 pages
File Size : 40,6 Mb
Release : 2016-04-19
Category : Medical
ISBN : 9781420084962

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Lennette's Laboratory Diagnosis of Viral Infections by Keith R Jerome Pdf

Written from the perspective of the diagnostician, this bestselling book is the definitive text on the laboratory diagnosis of human viral diseases. It contains a wealth of illustrations, tables, and algorithms to enhance your understanding of this ever-evolving field. The book is a ready reference for virologists, microbiologists, epidemiologists, laboratorians, and infections disease specialists, and students.

Author : David B. Collinge,Lisa Munk,B. Michael Cooke
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 48,6 Mb
Release : 2009-01-23
Category : Science
ISBN : 9781402087806

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Sustainable disease management in a European context by David B. Collinge,Lisa Munk,B. Michael Cooke Pdf

The main theme of the book is sustainable disease management in a European context. Some of the questions addressed are: How does society benefit from plant pathology research? How can new molecular approaches solve relevant problems in disease management? What other fields can we exploit in plant pathology research? What challenges are associated with free trade across the new borders? How can we contribute to solving problems of developing countries? How does plant pathology contribute to food quality and safety? How does globalization/internationalization affect teaching and extension in plant pathology?

Author : Institute of Medicine,Board on Global Health,Forum on Microbial Threats
Publisher : National Academies Press
Page : 284 pages
File Size : 49,5 Mb
Release : 2007-11-11
Category : Medical
ISBN : 9780309111140

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Global Infectious Disease Surveillance and Detection by Institute of Medicine,Board on Global Health,Forum on Microbial Threats Pdf

Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.

Author : John R. Stephenson,Alan Warnes
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 53,5 Mb
Release : 1998-08-28
Category : Medical
ISBN : 0896034798

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Diagnostic Virology Protocols by John R. Stephenson,Alan Warnes Pdf

A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

Author : H. A. Harfi,H. Nazer,William Oh,F. B. Stapleton,R. J. Whitley
Publisher : Springer Science & Business Media
Page : 4213 pages
File Size : 46,7 Mb
Release : 2012-01-10
Category : Medical
ISBN : 9783642022029

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Textbook of Clinical Pediatrics by H. A. Harfi,H. Nazer,William Oh,F. B. Stapleton,R. J. Whitley Pdf

The new edition of this classic reference offers a problem-based approach to pediatric diseases. It encompasses almost all pediatric subspecialties and covers every pediatric disease and organ system. It includes case studies and over 750 lavish illustrations.

Author : Susanne Modrow,Dietrich Falke,Uwe Truyen,Hermann Schätzl
Publisher : Springer
Page : 0 pages
File Size : 44,7 Mb
Release : 2013-09-18
Category : Science
ISBN : 3642207170

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Molecular Virology by Susanne Modrow,Dietrich Falke,Uwe Truyen,Hermann Schätzl Pdf

The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.

Author : Arndt Rolfs,Hermann C. Schumacher,Peter Marx
Publisher : Springer Science & Business Media
Page : 258 pages
File Size : 47,8 Mb
Release : 2013-12-01
Category : Medical
ISBN : 9783642759246

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PCR Topics by Arndt Rolfs,Hermann C. Schumacher,Peter Marx Pdf

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

Author : John R. Stephenson,Alan Warnes
Publisher : Humana Press
Page : 470 pages
File Size : 44,9 Mb
Release : 2010-12-02
Category : Medical
ISBN : 1607618168

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Diagnostic Virology Protocols by John R. Stephenson,Alan Warnes Pdf

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Author : Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders
Publisher : Taylor & Francis
Page : 362 pages
File Size : 47,8 Mb
Release : 2004
Category : Polymerase chain reaction
ISBN : 9781134184002

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Real-Time PCR by Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders Pdf

Author : Daniele Dionisio
Publisher : Springer Science & Business Media
Page : 504 pages
File Size : 51,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9788847020917

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Textbook-Atlas of Intestinal Infections in AIDS by Daniele Dionisio Pdf

This book represents an international collaborative work focused on the current challenges of AIDS-related intestinal infections in the worldwide scenario. The unique problems of developing countries, the epidemiological and immunological aspects, the risks for immunodeficient travellers and the reservoirs and ways of transmission from animal to man are all equally considered, as are the diagnostic advances and the changing patterns of prophylaxis and treatment. Moreover, the etiological and clinical aspects and the histologic and electron microscopic features of biopsy samples are reported. Furthermore, notes on intestinal parasitoses in the past centuries with emphasis on disadvantaged people, and worldwide trends and perspectives are also included as a link between the past and the future. This volume is expected to fill a gap in the medical literature, provide the latest information on therapeutic advances, provide an exhaustive series of light and electron microscopy micrographs and illustrations, contribute to the educational programs in developing countries, and gain international approval as a reference book for problems arising in clinical and laboratory practice, and as a text book for medical and graduate students.