The Polymerase Chain Reaction Pcr For Human Viral Diagnosis

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The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis

Jonathan P. Clewley
The Polymerase Chain Reaction PCR for Human Viral Diagnosis Book PDF

Author : Jonathan P. Clewley
Publisher : CRC Press
Page : 244 pages
File Size : 41,5 Mb
Release : 1994-10-27
Category : Science
ISBN : 0849348331

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The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis by Jonathan P. Clewley Pdf

The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.

Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Yechiel Becker,Gholamreza Darai
Diagnosis of Human Viruses by Polymerase Chain Reaction Technology Book PDF

Author : Yechiel Becker,Gholamreza Darai
Publisher : Springer Science & Business Media
Page : 428 pages
File Size : 41,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642847660

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology by Yechiel Becker,Gholamreza Darai Pdf

The basis for the effective treatment and cure of a patient is the rapid diagnosis of the disease and its causative agent, which is based on the analysis of the clinical symptoms coupled with laboratory tests. Although rapid advance ments have been made in the laboratory diagnosis of virus diseases, the neces sary isolation of the causative virus from the clinical specimens is a relatively long procedure. Viruses which integrate into the cellular DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are difficult to identify by molecular techniques, while viruses which exist in the clinical material in low concentrations are even more formidable to identify. Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (1985) led to a revolution in virus diagnosis. The peR technique was rapidly applied to the diagnosis of viruses in clinical material. Volume 1 of Frontiers of Virology provides new information on the advan tages of the use of the peR for the diagnosis of many human disease-causing viruses, as well as on some problems with its use.

Molecular Methods for Virus Detection

Danny L. Wiedbrauk,Daniel H. Farkas
Molecular Methods for Virus Detection Book PDF

Author : Danny L. Wiedbrauk,Daniel H. Farkas
Publisher : Elsevier
Page : 386 pages
File Size : 46,8 Mb
Release : 1995-02-08
Category : Science
ISBN : 0080536891

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Molecular Methods for Virus Detection by Danny L. Wiedbrauk,Daniel H. Farkas Pdf

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables

PCR: Protocols for Diagnosis of Human and Animal Virus Diseases

Yechiel Becker,Gholamreza Darai
PCR Protocols for Diagnosis of Human and Animal Virus Diseases Book PDF

Author : Yechiel Becker,Gholamreza Darai
Publisher : Springer
Page : 622 pages
File Size : 41,6 Mb
Release : 1995-09-26
Category : Medical
ISBN : UOM:39015040742853

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PCR: Protocols for Diagnosis of Human and Animal Virus Diseases by Yechiel Becker,Gholamreza Darai Pdf

Today the diagnosis of human and animal pathogenic viruses would be unthinkable without the polymerase chain reaction (PCR). The short-styled protocols in this volume elucidate this important technique and its application in the laboratory, including a discussion of recent advances in PCR technology. The enclosed diskette enables the user to work with interesting sequences on his own computer.

PCR Topics

Arndt Rolfs,Hermann C. Schumacher,Peter Marx
PCR Topics Book PDF

Author : Arndt Rolfs,Hermann C. Schumacher,Peter Marx
Publisher : Springer Science & Business Media
Page : 258 pages
File Size : 41,7 Mb
Release : 2013-12-01
Category : Medical
ISBN : 9783642759246

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PCR Topics by Arndt Rolfs,Hermann C. Schumacher,Peter Marx Pdf

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

PCR in Bioanalysis

Stephen J Meltzer
PCR in Bioanalysis Book PDF

Author : Stephen J Meltzer
Publisher : Springer Science & Business Media
Page : 286 pages
File Size : 47,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592595754

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PCR in Bioanalysis by Stephen J Meltzer Pdf

PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

Lennette's Laboratory Diagnosis of Viral Infections

Keith R Jerome
Lennette s Laboratory Diagnosis of Viral Infections Book PDF

Author : Keith R Jerome
Publisher : CRC Press
Page : 512 pages
File Size : 53,6 Mb
Release : 2016-04-19
Category : Medical
ISBN : 9781420084962

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Lennette's Laboratory Diagnosis of Viral Infections by Keith R Jerome Pdf

Written from the perspective of the diagnostician, this bestselling book is the definitive text on the laboratory diagnosis of human viral diseases. It contains a wealth of illustrations, tables, and algorithms to enhance your understanding of this ever-evolving field. The book is a ready reference for virologists, microbiologists, epidemiologists, laboratorians, and infections disease specialists, and students.

The Polymerase Chain Reaction

Kary B. Mullis
The Polymerase Chain Reaction Book PDF

Author : Kary B. Mullis
Publisher : Springer Science & Business Media
Page : 0 pages
File Size : 41,7 Mb
Release : 1994
Category : Medical
ISBN : 0817636072

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The Polymerase Chain Reaction by Kary B. Mullis Pdf

One: Methodology.- I. Basic Methodology.- 1. Manipulation of DNA by PCR.- 2. Cloning PCR Products.- 3. Optimization of Multiplex PCRs.- 4. Preparation of Nucleic Acids for Archival Material.- 5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ.- II. Quantitation.- 6. Quantitative PCR: An Overview.- 7. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control.- 8. RT-PCR and mRNA Quantitation.- 9. Analysis of Human T-Cell Repertoires by PCR.- III. Nonisotopic Detection.- 10. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview.- 11. Fluorescent Detection Methods for PCR Analysis.- 12. Enzyme-Labeled Oligonucleotides.- 13. Application of the Hybridization Protection Assay (HPA) to PCR.- IV. Instrumentation.- 14. PCR Instrumentation: Where Do We Stand?.- 15. Rapid Cycle DNA Amplification.- 16. Automating the PCR Process.- V. Sequencing.- 17. PCR and DNA Sequencing.- 18. Phage Promoter-Based Methods for Sequencing and Screening for Mutations.- 19. Capture PCR: An Efficient Method for Walking Along Chromosomal DNA and cDNA.- Two: Applications.- I. General Applications.- 20. In Vitro Evolution of Functional Nucleic Acids: High-Affinity RNA Ligands of the HIV-1 rev Protein.- 21. The Application of PCR to Forensic Science.- 22. Recreating the Past by PCR.- 23. Nonbiological Applications.- II. Genetic Analysis.- 24. RT-PCR and Gene Expression.- 25. Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs.- 26. Genetics, Plants, and the Polymerase Chain Reaction.- III. Assessment of Therapy Effectiveness.- 27. PCR Assessment of the Efficacy of Therapy in Philadelphia Chromosome-Positive Leukemias.- 28. The Detection of Minimal Residual Disease (MRD) in Acute Lymphoblastic Leukemia Using Clone-Specific Probes Directed against V(D)J Junctional Sequences.- 29. Assessment of Therapy Effectiveness: Infectious Disease.- 30. Gene Therapy.- IV. Diagnostics.- 31. PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes.- 32. Clinical Applications of the Polymerase Chain Reaction.- 33. Infectious Diseases.- Three: PCR and the World of Business.- 34. PCR in the Marketplace.- 35. PCR and Scientific Invention: The Trial of DuPont vs. Cetus.

PCR Protocols

Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White
PCR Protocols Book PDF

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White
Publisher : Academic Press
Page : 482 pages
File Size : 53,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080886718

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PCR Protocols by Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White Pdf

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications

PCR Technology

Henry Erlich
PCR Technology Book PDF

Author : Henry Erlich
Publisher : Springer
Page : 246 pages
File Size : 55,9 Mb
Release : 2015-12-31
Category : Science
ISBN : 9781349202355

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PCR Technology by Henry Erlich Pdf

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

Diagnostic Virology Protocols

John R. Stephenson,Alan Warnes
Diagnostic Virology Protocols Book PDF

Author : John R. Stephenson,Alan Warnes
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 45,7 Mb
Release : 1998-08-28
Category : Medical
ISBN : 0896034798

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Diagnostic Virology Protocols by John R. Stephenson,Alan Warnes Pdf

A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

PCR for Clinical Microbiology

Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday
PCR for Clinical Microbiology Book PDF

Author : Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday
Publisher : Springer Science & Business Media
Page : 438 pages
File Size : 47,6 Mb
Release : 2010-07-03
Category : Science
ISBN : 9789048190393

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PCR for Clinical Microbiology by Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday Pdf

Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology

PCR in Situ Hybridization

Gerard J. Nuovo
PCR in Situ Hybridization Book PDF

Author : Gerard J. Nuovo
Publisher : Lippincott Williams & Wilkins
Page : 536 pages
File Size : 47,7 Mb
Release : 1997
Category : Cytodiagnosis
ISBN : UOM:39015038533090

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PCR in Situ Hybridization by Gerard J. Nuovo Pdf

Describes the technique whereby the extreme sensitivity of the polymerase chain reaction (PCR) is combined with the cell localizing ability of in situ hybridization. This revised and updated edition contains chapters on the basics of molecular biology; the nonspecific pathways of PCR; applications of PCR in situ hybridization--human papillomavirus, and HIV-1; and instrumentation. There is also an appendix on reagents for molecular biological analyses. Annotation copyright by Book News, Inc., Portland, OR

Real-Time PCR

M Tevfik Dorak
Real Time PCR Book PDF

Author : M Tevfik Dorak
Publisher : Taylor & Francis
Page : 362 pages
File Size : 45,8 Mb
Release : 2007-01-24
Category : Medical
ISBN : 9781134184002

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Real-Time PCR by M Tevfik Dorak Pdf

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

The Polymerase Chain Reaction

Kary B. Mullis,Francois Ferre,Richard A. Gibbs
The Polymerase Chain Reaction Book PDF

Author : Kary B. Mullis,Francois Ferre,Richard A. Gibbs
Publisher : Springer Science & Business Media
Page : 464 pages
File Size : 50,6 Mb
Release : 2012-02-02
Category : Medical
ISBN : 9781461202578

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The Polymerase Chain Reaction by Kary B. Mullis,Francois Ferre,Richard A. Gibbs Pdf

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...