Physicochemical Methods In The Study Of Biomembranes

Author : Herwig J. Hilderson,Gregory B. Ralston
Publisher : Springer Science & Business Media
Page : 524 pages
File Size : 55,8 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461518631

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Physicochemical Methods in the Study of Biomembranes by Herwig J. Hilderson,Gregory B. Ralston Pdf

In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi tionally been applied to studies of model systems of lipids of well-defined com position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.

Author : Gertz I. Likhtenshtein
Publisher : Springer Nature
Page : 523 pages
File Size : 52,8 Mb
Release : 2021-10-21
Category : Science
ISBN : 9783030825034

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Biological Water by Gertz I. Likhtenshtein Pdf

This book embraces all physiochemical aspects of the structure and molecular dynamics of water, focusing on its role in biological objects, e.g. living cells and tissue, and in the formation of functionally active structures of biological molecules and their ensembles. Water is the single most abundant chemical found in all living things. It offers a detailed look into the latest modern physical methods for studying the molecular structure and dynamics of the water and provides a critical analysis of the existing literature data on the properties of water in biological objects. Water as a chemical reagent and as a medium for the formation of conditions for enzymatic catalysis is a core focus of this book. Although well suited for active researchers, the book as a whole, as well as each chapter on its own, can be used as fundamental reference material for graduate and undergraduate students throughout chemistry, physics, biophysics and biomedicine.

Author : J. Katsaras,T. Gutberlet
Publisher : Springer Science & Business Media
Page : 304 pages
File Size : 44,5 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783662044964

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Lipid Bilayers by J. Katsaras,T. Gutberlet Pdf

Provides the reader with an up to date insight of the current state of the art in the field of lipid bilayer research and the important insights derived for the understanding of the complex and varied behaviour of biological membranes and its function.

Author : Ronald J. Clarke,Mohammed A. A. Khalid
Publisher : John Wiley & Sons
Page : 488 pages
File Size : 46,9 Mb
Release : 2015-10-12
Category : Science
ISBN : 9781118858806

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Pumps, Channels and Transporters by Ronald J. Clarke,Mohammed A. A. Khalid Pdf

Describes experimental methods for investigating the function of pumps, channels and transporters Covers new emerging analytical methods used to study ion transport membrane proteins such as single-molecule spectroscopy Details a wide range of electrophysiological techniques and spectroscopic methods used to analyze the function of ion channels, ion pumps and transporters Covers state-of-the art analytical methods to study ion pumps, channels, and transporters, and where analytical chemistry can make further contributions

Author : Hans J. Vogel
Publisher : Springer Science & Business Media
Page : 415 pages
File Size : 53,9 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591848

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Calcium-Binding Protein Protocols by Hans J. Vogel Pdf

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Author : Peter J. Quinn,Valerian E. Kagan
Publisher : Springer Science & Business Media
Page : 340 pages
File Size : 51,9 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306479311

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Phospholipid Metabolism in Apoptosis by Peter J. Quinn,Valerian E. Kagan Pdf

The last few years have witnessed an explosion of both interest and knowledge about apoptosis, the process by which a cell actively commits suicide. The number of publications on the topic has increased from nothing in the early 1980s to more than 10,000 papers annually today. It is now well recognized that apoptosis is essential in many aspects of normal development and is required for maintaining tissue homeostasis. The idea that life requires death seems somewhat paradoxical, but cell suicide is essential for an animal to survive. For example, without selective destruction of “non-self” T cells, an animal would lack immunity. Similarly, meaningful neural connections in the brain are whittled from a mass of cells. Further, developmental cell remodeling during tissue maturation involves programmed cell death as the major mechanism for functional and structural safe transition of undifferentiated cells to more specialized counterparts. Apoptosis research, with roots in biochemistry, developmental and cell biology, genetics, and immunology, embraces this long-ignored natural law. Failure to properly regulate apoptosis can have catastrophic consequences. Cancer and many diseases (AIDS, Alzheimer’s disease, Parkinson’s disease, heart attack, stroke, etc. ) are thought to arise from deregulation of apoptosis. As apoptosis emerges as a key biological regulatory mechanism, it has become harder and harder to keep up with new developments in this field.

Author : Miguel A. R. B. Castanho
Publisher : Internat'l University Line
Page : 675 pages
File Size : 40,7 Mb
Release : 2010
Category : Medical
ISBN : 9780972077453

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Membrane-active Peptides by Miguel A. R. B. Castanho Pdf

Author : Michelina Catauro
Publisher : MDPI
Page : 216 pages
File Size : 47,6 Mb
Release : 2019-10-14
Category : Science
ISBN : 9783039213535

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Sol-Gel Chemistry Applied to Materials Science by Michelina Catauro Pdf

Sol–gel technology is a contemporary advancement in science that requires taking a multidisciplinary approach with regard to its various applications. This book highlights some applications of the sol–gel technology, including protective coatings, catalysts, piezoelectric devices, wave guides, lenses, high-strength ceramics, superconductors, synthesis of nanoparticles, and insulating materials. In particular, for biotechnological applications, biomolecules or the incorporation of bioactive substances into the sol–gel matrix has been extensively studied and has been a challenge for many researchers. Some sol–gel materials are widely applied in light-emitting diodes, solar cells, sensing, catalysis, integration in photovoltaic devices, and more recently in biosensing, bioimaging, or medical diagnosis; others can be considered excellent drug delivery systems. The goal of an ideal drug delivery system is the prompt delivery of a therapeutic amount of the drug to the proper site in the body, where the desired drug concentration can be maintained. The interactions between drugs and the sol–gel system can affect the release rate. In conclusion, the sol–gel synthesis method offers mixing at the molecular level and is able to improve the chemical homogeneity of the resulting composite. This opens new doors not only regarding compositions of previously unattainable materials, but also to unique structures with different applications.

Author : Otto Holst
Publisher : Springer Science & Business Media
Page : 378 pages
File Size : 49,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590520

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Bacterial Toxins by Otto Holst Pdf

The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial toxins need to be acquainted with all these disciplines in order to work effectively in the field. To date, there has been no published collection offering detailed descr- tions of the techniques and methods needed by researchers operating across the field’sdiverse areas. The present volume Bacterial Toxins: Methods and Pro- cols, is intended to fill this gap. Bacterial Toxins: Methods and Protocols consists of two sections: one on protein toxins (15 chapters) and one on endotoxins (5 chapters). Each s- tion is introduced by an overview article (Chapters 1 and 16). The protocols collected represent state-of-the-art techniques that each have high impact on future bacterial toxin research. All methods are described by authors who have regularly been using the protocol in their own laboratories. Included in each chapter is a brief introduction to the method being described.

Author : Eric Dickinson,Ton Van Vliet
Publisher : Royal Society of Chemistry
Page : 426 pages
File Size : 52,9 Mb
Release : 2007-10-31
Category : Technology & Engineering
ISBN : 9781847550835

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Food Colloids, Biopolymers and Materials by Eric Dickinson,Ton Van Vliet Pdf

Food scientists aim to control the taste and texture of existing food products and to formulate new structures of high quality using novel combinations of ingredients and processing methods. Food Colloids, Biopolymers and Materials describes the physical chemistry and material science underlying the formulation and behaviour of multi-phase food systems and includes: * descriptions of new experimental techniques * recent food colloids research findings * authoritative overviews of conceptual issues Essential new findings are presented and emphasis is placed on the interfacial and gelation properties of food proteins, and the role of colloidal and biopolymer interactions in determining the properties of emulsions, dispersions, gels and foams. Specific topics include: confocal microscopy; diffusing wave spectroscopy; protein-polysaccharide interactions; biopolymer phase separation; fat crystallization; bubble/droplet coalescence; and bulk and surface rheology. This book is the latest addition to the highly regarded food colloid series published by the Royal Society of Chemistry and is of relevance to those working and researching in food science and surface and colloid science.

Author : Richard H. Templer,Robin Leatherbarrow
Publisher : Royal Society of Chemistry
Page : 298 pages
File Size : 47,8 Mb
Release : 2002
Category : Medical
ISBN : 0854048510

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Biophysical Chemistry by Richard H. Templer,Robin Leatherbarrow Pdf

In the post-genomic world, advances in the comprehension of cell behaviour will depend upon scientists deciphering the molecular basis of interactions between proteins and membranes. Bringing together contributions from chemists, biologists and physicists, Biophysical Chemistry: Membranes and Proteins demonstrates how multidisciplinary teams can gain insights into understanding complex biological systems. This book reflects both the scope and the interdisciplinary nature of the field, with topics including: modelling of biological systems; membrane structure and interactions; probing biomolecules; and channels and receptors. Full of stimulating articles and opinions, readers from academia and industry will welcome the wide range of coverage and the state-of-the-art science.

Author : John B. Lloyd,Robert W. Mason
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 40,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461558330

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Biology of the Lysosome by John B. Lloyd,Robert W. Mason Pdf

Volume 27 provides a comprehensive review of current knowledge of lysosome function in mammalian cells. The book's unique contribution is its series of chapters that offers unparalleled treatment of the metabolic activities of lysosomes.

Author : J. Robin Harris
Publisher : Springer Science & Business Media
Page : 446 pages
File Size : 50,9 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461303251

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Subcellular Biochemistry by J. Robin Harris Pdf

In Volume 25, leading experts present studies on the value of increased ascorbic acid intake and explore its specific contributions to human and animal health.

Author : B.B. Biswas,Siddhartha Roy
Publisher : Springer Science & Business Media
Page : 451 pages
File Size : 47,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9781489917270

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Proteins: Structure, Function, and Engineering by B.B. Biswas,Siddhartha Roy Pdf

Here, researchers review the latest breakthroughs in protein research. Their contributions explore emerging principles and techniques and survey important classes of proteins that will play key roles in the field's future. Articles examine the possibility of a Boltzman-like distribution in protein substructures, the new technique of Raman spectroscopy, and compact intermediate states of protein folding. This well-illustrated volume also features coverage of proteins that bind nucleic acids.

Author : B.B. Biswas,H.K. Das
Publisher : Springer Science & Business Media
Page : 455 pages
File Size : 47,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781489917072

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Plant-Microbe Interactions by B.B. Biswas,H.K. Das Pdf

Recent years have seen tremendous progress in unraveling the molecular basis of different plant-microbe interactions. Knowledge has accumulated on the mecha nisms of the microbial infection of plants, which can lead to either disease or resistance. The mechanisms developed by plants to interact with microbes, whether viruses, bacteria, or fungi, involve events that can lead to symbiotic association or to disease or tumor formation. Cell death caused by pathogen infection has been of great interest for many years because of its association with plant resistance. There appear to be two types of plant cell death associated with pathogen infection, a rapid hypersensitive cell death localized at the site of infection during an incompatible interaction between a resistant plant and an avirulent pathogen, and a slow, normosensitive plant cell death that spreads beyond the site of infection during some compatible interactions involving a susceptible plant and a virulent, necrogenic pathogen. Plants possess a number of defense mechanisms against infection, such as (i) production of phytoalexin, (ii) formation of hydrolases, (iii) accumulation of hydroxyproline-rich glycoprotein and lignin deposition, (iv) production of pathogen-related proteins, (v) produc tion of oligosaccharides, jasmonic acid, and various other phenolic substances, and (vi) production of toxin-metabolizing enzymes. Based on these observations, insertion of a single suitable gene in a particular plant has yielded promising results in imparting resistance against specific infection or disease. It appears that a signal received after microbe infection triggers different signal transduction pathways.