Plant Gene Silencing

Author : Kirankumar S. Mysore,Muthappa Senthil-Kumar
Publisher : Humana
Page : 0 pages
File Size : 49,8 Mb
Release : 2016-10-05
Category : Science
ISBN : 1493948474

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Plant Gene Silencing by Kirankumar S. Mysore,Muthappa Senthil-Kumar Pdf

This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.

Author : M.A. Matzke,A.J.M. Matzke
Publisher : Springer Science & Business Media
Page : 297 pages
File Size : 55,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401141833

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Plant Gene Silencing by M.A. Matzke,A.J.M. Matzke Pdf

This book is an up-to-date and comprehensive collection of reviews on various aspects of epigenetic gene silencing in plants. Research on this topic has undergone explosive growth during the past decade and has revealed novel features of gene regulation and plant defense responses that also apply to animals and fungi. Gene silencing is relevant for agricultural biotechnology because stable expression of transgenes is required for the successful commercialization of genetically engineered crops. The reviews have been written by distinguished authors who have made significant contributions to plant gene silencing research. This volume supersedes other books on gene silencing by focussing on plant systems, where many pioneering experiments have been performed, and by including the latest developments from top laboratories. The book is geared toward advanced students of genetics and plant sciences as well as applied and basic research scientists who work with transgenic organisms and epigenetic regulation of gene expression.

Author : Tamas Dalmay
Publisher : CABI
Page : 223 pages
File Size : 49,7 Mb
Release : 2017-05-29
Category : Science
ISBN : 9781780647678

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Plant Gene Silencing by Tamas Dalmay Pdf

Plant gene silencing is a crucially important phenomenon in gene expression and epigenetics. This book describes the way small RNA is produced and acts to silence genes, its likely origins in defence against viruses, and also its potential to improve plants. Plant gene silencing can be used to improve industrial traits, make plants more nutritious or more valuable to consumers, to remove allergens, and to improve resistance to weeds and pathogens.

Author : Peter Meyer
Publisher : Springer Science & Business Media
Page : 237 pages
File Size : 47,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642791451

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Gene Silencing in Higher Plants and Related Phenomena in Other Eukaryotes by Peter Meyer Pdf

In recent years several different gene silencing phenomena have been discovered in plants. The book summarizes the most recent data on gene silencing phenomena such as trans-, inactivation, paramutation and co-suppression. Plant researchers will find this edition a valuable help in differentiating between a number of puzzling and partly contradictory gene silencing events. Those not familiar with plant molecular biology are introduced into the relevant methods and scientific models. In addition examples and models of gene silencing in flamentous fungi, Drosophila and mammalian systems are presented. By providing a comparative update on gene silencing effects in different eukaryotes, this book should stimulate communication among scientists working in diverse areas of eukaryotic gene regulation.

Author : Charles H. Calisher,M.C. Horzinek
Publisher : Springer Science & Business Media
Page : 222 pages
File Size : 44,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783709164259

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100 Years of Virology by Charles H. Calisher,M.C. Horzinek Pdf

One hundred years ago, when Martinus W. Beijerinck in Delft and Friedrich Loeffler on Riems Island discovered a new class of infectious agents in plants and animals, a new discipline was born. This book, a compilation of papers written by well-recognized scientists, gives an impression of the early days, the pioneer period and the current state of virology. Recent developments and future perspectives of this discipline are sketched against a historic background. With contributions by A. Alcami, D. Baulcombe, F. Brown, L. W. Enquist, H. Feldmann, A. Garcia-Sastre, D. Griffiths, M. C. Horzinek, A. van Kammen, H.-D. Klenk, F. A. Murphy, T. Muster, R. O'Neill, P. Palese, C. Patience, R. Rott, H.- P. Schmiedebach, S. Schneider-Schaulies, G. L. Smith, J. A. Symons, Y. Takeuchi, V. ter Meulen, P. J. W. Venables, V. E. Volchkov, V. A. Volchkova, R. A. Weiss, W. Wittmann, H. Zheng.

Author : Annette Becker
Publisher : Humana Press
Page : 0 pages
File Size : 47,8 Mb
Release : 2013-02-06
Category : Medical
ISBN : 1627032770

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Virus-Induced Gene Silencing by Annette Becker Pdf

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.

Author : Volker A. Erdmann,Jan Barciszewski
Publisher : Springer Science & Business Media
Page : 510 pages
File Size : 50,8 Mb
Release : 2011-07-20
Category : Science
ISBN : 3642194540

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Non Coding RNAs in Plants by Volker A. Erdmann,Jan Barciszewski Pdf

In the most recent years, each of the RNA silencing pathways of plants have appeared to generate ncRNAs with dedicated functions, specialized biological activities and specific functional scopes. RNA silencing plays a crucial role in coordinating the expression, stability, protection and inheritance of eukaryotic genomes. It compromises several mechanisms, that invariably depend on core small non coding RNAs and that achieve dedicated sequence-specific functions. RNA silencing has been recognized to carry critical developmental, stress-response and bodyguard functions be coordinating the expression, protection, stability and inheritance of virtually all eukaryotic genomes. Thus, the ncRNAs encompass a wide set of mechanisms that achieve specialized functions.

Author : Erich Grotewold,Joseph Chappell,Elizabeth A. Kellogg
Publisher : John Wiley & Sons
Page : 264 pages
File Size : 40,6 Mb
Release : 2015-04-27
Category : Science
ISBN : 9781118539378

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Plant Genes, Genomes and Genetics by Erich Grotewold,Joseph Chappell,Elizabeth A. Kellogg Pdf

Plant Genes, Genomes and Genetics provides a comprehensive treatment of all aspects of plant gene expression. Unique in explaining the subject from a plant perspective, it highlights the importance of key processes, many first discovered in plants, that impact how plants develop and interact with the environment. This text covers topics ranging from plant genome structure and the key control points in how genes are expressed, to the mechanisms by which proteins are generated and how their activities are controlled and altered by posttranslational modifications. Written by a highly respected team of specialists in plant biology with extensive experience in teaching at undergraduate and graduate level, this textbook will be invaluable for students and instructors alike. Plant Genes, Genomes and Genetics also includes: specific examples that highlight when and how plants operate differently from other organisms special sections that provide in-depth discussions of particular issues end-of-chapter problems to help students recapitulate the main concepts rich, full-colour illustrations and diagrams clearly showing important processes in plant gene expression a companion website with PowerPoint slides, downloadable figures, and answers to the questions posed in the book Aimed at upper level undergraduates and graduate students in plant biology, this text is equally suited for advanced agronomy and crop science students inclined to understand molecular aspects of organismal phenomena. It is also an invaluable starting point for professionals entering the field of plant biology.

Author : Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet
Publisher : Frontiers Media SA
Page : 103 pages
File Size : 49,6 Mb
Release : 2021-07-28
Category : Science
ISBN : 9782889711000

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Advances and Challenges of RNAi Based Technologies for Plants by Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet Pdf

Author : J. Paszkowski
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 43,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401110945

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Homologous Recombination and Gene Silencing in Plants by J. Paszkowski Pdf

Higher eukaryotes are characterized by the allocation of distinct functions to numerous types of differentiated cells. Whereas in animals the well-defined, protected cells of the germ line separate early, germ cells in plants differentiate from somatic cells only after many cycles of mitotic division. Therefore somatic mutations in plants can be transmitted via the germ cells to the progeny. There is thus a clear need for somatic tissues to maintain their genetic integrity in the face of environmental challenges, and two types of interactions have been shown to play important roles in the conservation as well as flexibility of plant genomes: homologous recombination of repeated sequences and silencing of multiplied genes. Sensitive methods have been developed that allow greater insights into the dynamics of the genome. This book summarizes current knowledge and working hypotheses about the frequencies and mechanisms of mitochondrial, plastid, nuclear and viral recombination and the inactivation of repeated genes in plants. Despite rapid developments in the field, it is often not possible to provide final answers. Thus, it is an additional task of this book to define the open questions and future challenges. The book is addressed to scientists working on plant biology and recombination, to newcomers in the field and to advanced biology students.

Author : Praveen Guleria,Vineet Kumar
Publisher : Academic Press
Page : 638 pages
File Size : 44,6 Mb
Release : 2020-02-19
Category : Science
ISBN : 9780128173367

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Plant Small RNA by Praveen Guleria,Vineet Kumar Pdf

Plant Small RNA: Biogenesis, Regulation and Application describes the biosynthesis of small RNA in plant systems. With an emphasis on the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival, this books presents the basics and most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. In addition, it emphasizes the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival. Final sections cover the most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. Presents foundational information about small RNA biology and regulation in plants Includes small RNA pathway advances Describes the application and scope of small RNA technology for agricultural stability

Author : Gregory J. Hannon
Publisher : CSHL Press
Page : 568 pages
File Size : 49,5 Mb
Release : 2003
Category : Gene silencing
ISBN : 0879696419

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RNAi by Gregory J. Hannon Pdf

In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.

Author : Vincent Courdavault,Sébastien Besseau
Publisher : Humana
Page : 261 pages
File Size : 44,7 Mb
Release : 2021-07-03
Category : Science
ISBN : 1071607537

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Virus-Induced Gene Silencing in Plants by Vincent Courdavault,Sébastien Besseau Pdf

This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : Annette Becker
Publisher : Humana Press
Page : 236 pages
File Size : 52,7 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493959425

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Virus-Induced Gene Silencing by Annette Becker Pdf

Maintaining the high standards and detailed practical guidance of Springer s Methods in Molecular Biology series, this book has comprehensive, step-by-step protocols on the fast and reliable use of viruses to induce down-regulation of target gene expression."

Author : Dr. Tim Doran,Chris Helliwell
Publisher : CABI
Page : 257 pages
File Size : 42,7 Mb
Release : 2009
Category : Science
ISBN : 9781845934101

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RNA Interference by Dr. Tim Doran,Chris Helliwell Pdf

This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.