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Author : Kumar
Publisher : Unknown
Page : 128 pages
File Size : 40,8 Mb
Release : 2009-01-01
Category : Electronic
ISBN : 8172335938
Author : S.S. Bhojwani,M.K. Razdan
Publisher : Elsevier
Page : 766 pages
File Size : 47,8 Mb
Release : 1996-11-08
Category : Science
ISBN : 0080539092
Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature. This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.
Author : Shailesh Kumar,Sweta Mishra,A.P. Mishra
Publisher : Scientific Publishers
Page : 211 pages
File Size : 43,6 Mb
Release : 2016-01-01
Category : Technology & Engineering
ISBN : 9789386102201
Biotechnology is an emerging field of science and as such the government of India is laying a large and exclusive impetus on it. Plant tissue culture is the basic and the most important aspect of Biotechnology. Therefore, plant tissue culture has been introduced as a compulsory course in the Undergraduate and Postgraduate syllabi of all the Agricultural Universities, ICAR institutes and other plant science related educational organizations. This book has been designed to benefit the students, the research scholars and the scientists for developing a level of self-confidence to conduct the experiments independently and can acquire the practical skills along with the basic know-how about the techniques being used. Each chapter is devoted to a separate aspect of plant tissue culture and the chapters are arranged in the order of increasing technical complexity. The opening chapters present a brief historical survey of the field of plant tissue culture, a background in sterilization techniques. The text deals with the experimental details of each and every technique. The protocols have been simplified legibly to include details and notes that we hope will help the user avoid unnecessary errors and confusion. All the applications of plant tissue culture have been very well discussed and the techniques associated with them described in detail. This being a comp-lete book on Plant tissue culture will solve all types of problem of the users who will not have to use other resource books for the same purpose.
Author : Roberta H. Smith
Publisher : Academic Press
Page : 206 pages
File Size : 47,8 Mb
Release : 2013
Category : Science
ISBN : 9780124159204
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Author : B. N. Sathyanarayana,Dalia B. Varghese
Publisher : I. K. International Pvt Ltd
Page : 299 pages
File Size : 55,9 Mb
Release : 2007
Category : Botany, Experimental
ISBN : 9788189866112
Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
Author : Indra K. Vasil,Trevor A. Thorpe
Publisher : Springer Science & Business Media
Page : 593 pages
File Size : 42,9 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401726818
Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.
Author : Robert H. Smith
Publisher : Academic Press
Page : 190 pages
File Size : 48,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323160476
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Author : S.S. Bhojwani
Publisher : Elsevier
Page : 470 pages
File Size : 40,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780444598479
During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.
Author : John H. Dodds,Lorin W. Roberts
Publisher : International Potato Center
Page : 260 pages
File Size : 50,5 Mb
Release : 1985-10-31
Category : Science
ISBN : 0521315166
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
Author : T. Pullaiah,M.V. Subba Rao,E. Sreedevi
Publisher : Scientific Publishers
Page : 249 pages
File Size : 55,5 Mb
Release : 2017-03-01
Category : Technology & Engineering
ISBN : 9789387869806
Plant Tissue Culture, Second Edition is accompanied with new exercises demonstrating new arrays along with information on development of a customized protocol for protoplast isolation, suspension, haploid cultures, secondary metabolite production, and cryopreservation techniques. All experimental systems are written clear and easy-to-understand manner with the text being well-documented along with detailed drawings containing the plant tissue culture requirements for each particular application. Besides addressing recent advancements on wide variety of topics of Plant Tissue Culture, it gives the practical and technical knowledge required to train the next generation of plant scientists regardless of their ultimate specialization. It includes the complements of both theory and experiments. Plant Scientists, teachers and students will benefit greatly from this clearly presented tissue culture techniques that guides reader from lab setup to supplies, stock solution and media preparation, measurements, explant selection and disinfestations, along with their experimental observations.
Author : Robert N. Trigiano
Publisher : Routledge
Page : 470 pages
File Size : 46,6 Mb
Release : 2018-04-27
Category : Science
ISBN : 9781351424035
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
Author : Sunghun Park
Publisher : Academic Press
Page : 254 pages
File Size : 46,5 Mb
Release : 2021-02-17
Category : Science
ISBN : 9780323851374
Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012
Author : Kenneth C. Torres
Publisher : Springer Science & Business Media
Page : 282 pages
File Size : 52,8 Mb
Release : 2012-12-06
Category : Technology & Engineering
ISBN : 9781461597568
This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.
Author : Robert N. Trigiano,Dennis J. Gray
Publisher : CRC Press
Page : 608 pages
File Size : 53,5 Mb
Release : 2016-03-30
Category : Medical
ISBN : 9781439896143
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Author : M. K. Razdan
Publisher : Science Publishers
Page : 394 pages
File Size : 46,8 Mb
Release : 2003
Category : Science
ISBN : 1578082374
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.