Practical Methods In Molecular Biology

Author : Robert F. Schleif,Pieter C. Wensink
Publisher : Springer Science & Business Media
Page : 229 pages
File Size : 40,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461259565

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Practical Methods in Molecular Biology by Robert F. Schleif,Pieter C. Wensink Pdf

This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.

Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Page : 220 pages
File Size : 45,5 Mb
Release : 2020-10-28
Category : Science
ISBN : 9780128244500

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Advanced Methods in Molecular Biology and Biotechnology by Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool Pdf

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Author : Bal Ram Singh,Raj Kumar
Publisher : Cambridge University Press
Page : 378 pages
File Size : 42,8 Mb
Release : 2022-06-16
Category : Medical
ISBN : 9781108486408

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Practical Techniques in Molecular Biotechnology by Bal Ram Singh,Raj Kumar Pdf

The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.

Author : Robert F Schleif,Pieter C Wensink
Publisher : Unknown
Page : 236 pages
File Size : 46,5 Mb
Release : 1981-11-30
Category : Electronic
ISBN : 1461259576

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Practical Methods in Molecular Biology by Robert F Schleif,Pieter C Wensink Pdf

Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 44,9 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459

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Molecular Biology Techniques by Heather Miller,D. Scott Witherow,Sue Carson Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 48,7 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

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Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
Page : 434 pages
File Size : 50,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642569685

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Basic Techniques in Molecular Biology by Stefan Surzycki Pdf

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Author : Oksana Ableitner
Publisher : Springer Nature
Page : 93 pages
File Size : 51,9 Mb
Release : 2022-01-07
Category : Science
ISBN : 9783658339203

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Introduction to Molecular Biology by Oksana Ableitner Pdf

Oksana Ableitner offers a practical, clearly structured and easy to understand introduction to complicated definitions and structures in chemistry and molecular biology for work in the molecular biology laboratory. The author is guided by her experience in working with students and uses many illustrations to visualize abstract knowledge. An understanding of this matter is an essential basis for successful work with DNA and RNA in order to ensure high quality results. For responsible activities in application - such as genetic research or the determination of various pathogens - it is essential to be confident in dealing with the basics of these sensitive, fast and specific analytical methods. This Springer essential is a translation of the original German 2nd edition essentials, Einführung in die Molekularbiologie by Oksana Ableitner, published by Springer Fachmedien Wiesbaden GmbH, part of Springer Nature in 2018. The translation was done with the help of artificial intelligence (machine translation by the serviceDeepL.com). A subsequent human revision was done primarily in terms of content, so that the book will read stylistically differently from a conventional translation. Springer Nature works continuously to further the development of tools for the production of books and on the related technologies to support the authors.

Author : Ricardo V. Lloyd
Publisher : Springer Science & Business Media
Page : 435 pages
File Size : 52,6 Mb
Release : 2001-06-15
Category : Medical
ISBN : 9781592591909

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Morphology Methods by Ricardo V. Lloyd Pdf

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Author : John R. Crowther
Publisher : Springer Science & Business Media
Page : 231 pages
File Size : 55,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592595297

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ELISA by John R. Crowther Pdf

ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

Author : Friedhelm Hildebrandt,Peter Igarashi
Publisher : Springer Science & Business Media
Page : 394 pages
File Size : 42,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783642598111

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Techniques in Molecular Medicine by Friedhelm Hildebrandt,Peter Igarashi Pdf

This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual

Author : Russell Schwartz
Publisher : MIT Press
Page : 403 pages
File Size : 53,5 Mb
Release : 2008-07-25
Category : Science
ISBN : 9780262195843

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Biological Modeling and Simulation by Russell Schwartz Pdf

A practice-oriented survey of techniques for computational modeling and simulation suitable for a broad range of biological problems. There are many excellent computational biology resources now available for learning about methods that have been developed to address specific biological systems, but comparatively little attention has been paid to training aspiring computational biologists to handle new and unanticipated problems. This text is intended to fill that gap by teaching students how to reason about developing formal mathematical models of biological systems that are amenable to computational analysis. It collects in one place a selection of broadly useful models, algorithms, and theoretical analysis tools normally found scattered among many other disciplines. It thereby gives the aspiring student a bag of tricks that will serve him or her well in modeling problems drawn from numerous subfields of biology. These techniques are taught from the perspective of what the practitioner needs to know to use them effectively, supplemented with references for further reading on more advanced use of each method covered. The text, which grew out of a class taught at Carnegie Mellon University, covers models for optimization, simulation and sampling, and parameter tuning. These topics provide a general framework for learning how to formulate mathematical models of biological systems, what techniques are available to work with these models, and how to fit the models to particular systems. Their application is illustrated by many examples drawn from a variety of biological disciplines and several extended case studies that show how the methods described have been applied to real problems in biology.

Author : Keith Wilson,Andreas Hofmann,John M. Walker,Samuel Clokie
Publisher : Cambridge University Press
Page : 961 pages
File Size : 51,5 Mb
Release : 2018-04-19
Category : Medical
ISBN : 9781107162273

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Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,Andreas Hofmann,John M. Walker,Samuel Clokie Pdf

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Author : Stefan Surzycki
Publisher : Springer
Page : 434 pages
File Size : 55,5 Mb
Release : 2000-04-27
Category : Science
ISBN : 3540666788

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Basic Techniques in Molecular Biology by Stefan Surzycki Pdf

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Author : Heejung Bang,Xi Kathy Zhou,Heather L. van Epps,Madhu Mazumdar
Publisher : Humana
Page : 636 pages
File Size : 49,6 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493961241

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Statistical Methods in Molecular Biology by Heejung Bang,Xi Kathy Zhou,Heather L. van Epps,Madhu Mazumdar Pdf

This progressive book presents the basic principles of proper statistical analyses. It progresses to more advanced statistical methods in response to rapidly developing technologies and methodologies in the field of molecular biology.