Probing The Ubiquitin Landscape

Author : Huib Ovaa,Monique P. C. Mulder,Zhihao Zhuang,Benedikt M. Kessler,Lei Liu
Publisher : Frontiers Media SA
Page : 205 pages
File Size : 54,9 Mb
Release : 2020-12-11
Category : Science
ISBN : 9782889638253

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Probing the Ubiquitin Landscape by Huib Ovaa,Monique P. C. Mulder,Zhihao Zhuang,Benedikt M. Kessler,Lei Liu Pdf

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Author : Victor M. Bolanos-Garcia,Julien Licchesi,Heike Laman,Fumiyo Ikeda
Publisher : Frontiers Media SA
Page : 180 pages
File Size : 49,6 Mb
Release : 2020-07-22
Category : Science
ISBN : 9782889638833

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E3 Ubiquitin Ligases: From Structure to Physiology by Victor M. Bolanos-Garcia,Julien Licchesi,Heike Laman,Fumiyo Ikeda Pdf

Author : Anonim
Publisher : BoD – Books on Demand
Page : 126 pages
File Size : 44,9 Mb
Release : 2020-12-09
Category : Science
ISBN : 9781838804329

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Ubiquitin by Anonim Pdf

The ubiquitin-proteasome pathway consists of ubiquitin, substrate proteins, E1 enzymes, E2 enzymes, E3 enzymes, and proteasome, which acts in a series of enzymatic reaction chains to ubiquitinate substrate proteins such as surplus and misfolded proteins for degradation by the proteasome to maintain the balance between protein synthesis and degradation in a cell and tissue. Moreover, deubiquitinating enzymes can remove the attached ubiquitin chain, which results in a reverse ubiquitination-deubiquitination process involved in multiple biological processes in a cell and tissue. The changes of components in the ubiquitin-proteasome pathway are associated with multiple pathophysiological processes, such as cancers and neurodegenerative diseases. This book presents the new advances in concepts, analytical methodology, and application of ubiquitin-proteasome pathway for clarification of molecular mechanisms and discovery of new therapeutic targets and drugs in different diseases.

Author : Xuechen Li
Publisher : Springer Nature
Page : 258 pages
File Size : 49,8 Mb
Release : 2022-06-27
Category : Science
ISBN : 9781071624890

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Chemical Protein Synthesis by Xuechen Li Pdf

This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author : Bharat Bhushan
Publisher : Springer Science & Business Media
Page : 634 pages
File Size : 40,9 Mb
Release : 2012-10-24
Category : Science
ISBN : 9783642254147

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Scanning Probe Microscopy in Nanoscience and Nanotechnology 3 by Bharat Bhushan Pdf

This book presents the physical and technical foundation of the state of the art in applied scanning probe techniques. It constitutes a timely and comprehensive overview of SPM applications. The chapters in this volume relate to scanning probe microscopy techniques, characterization of various materials and structures and typical industrial applications, including topographic and dynamical surface studies of thin-film semiconductors, polymers, paper, ceramics, and magnetic and biological materials. The chapters are written by leading researchers and application scientists from all over the world and from various industries to provide a broader perspective.

Author : Nelu Grinberg,Peter W. Carr
Publisher : CRC Press
Page : 225 pages
File Size : 52,8 Mb
Release : 2024-08-20
Category : Science
ISBN : 9781040097717

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Advances in Chromatography by Nelu Grinberg,Peter W. Carr Pdf

For six decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. The clear presentation of topics and vivid illustrations for which this series has become known make the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill. Presents an in-depth review of chaotropic chromatography. Reviews recent advances in HPLC and SFC columns packed with hybrid organic/inorganic particles. Explores new advances in nano-liquid chromatography for proteomics analysis. Outlines the heterogeneity of gangliosides and advances in the chromatographic techniques used in their separation.

Author : Anonim
Publisher : Elsevier
Page : 4266 pages
File Size : 54,7 Mb
Release : 2020-07-22
Category : Science
ISBN : 9780081026915

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Comprehensive Natural Products III by Anonim Pdf

Comprehensive Natural Products III, Third Edition, Seven Volume Set updates and complements the previous two editions, including recent advances in cofactor chemistry, structural diversity of natural products and secondary metabolites, enzymes and enzyme mechanisms and new bioinformatics tools. Natural products research is a dynamic discipline at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids and enzymes. This book reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine and to stimulate new ideas among the established natural products community. Provides readers with an in-depth review of current natural products research and a critical insight into the future direction of the field Bridges the gap in knowledge by covering developments in the field since the second edition published in 2010 Split into 7 sections on key topics to allow students, researchers and professionals to find relevant information quickly and easily Ensures that the knowledge within is easily understood by and applicable to a large audience

Author : Bharat Bhushan
Publisher : Springer Science & Business Media
Page : 975 pages
File Size : 42,6 Mb
Release : 2010-01-22
Category : Technology & Engineering
ISBN : 9783642035357

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Scanning Probe Microscopy in Nanoscience and Nanotechnology by Bharat Bhushan Pdf

This book presents the physical and technical foundation of the state-of-the-art in applied scanning probe techniques. It constitutes a comprehensive overview of SPM applications. The chapters are written by leading researchers and application scientists.

Author : Julie Maupin-Furlow,Mariola J. Edelmann
Publisher : Springer Nature
Page : 307 pages
File Size : 40,6 Mb
Release : 2022-11-09
Category : Science
ISBN : 9781071628034

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Deubiquitinases by Julie Maupin-Furlow,Mariola J. Edelmann Pdf

This book aims to advance the understanding of deubiquitinases (DUBs) and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches that enable both the determination and alteration of DUB biological function.. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Deubiquitinases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author : Manuel S Rodriguez,Rosa Barrio
Publisher : Springer Nature
Page : 245 pages
File Size : 55,8 Mb
Release : 2022-11-29
Category : Science
ISBN : 9781071628591

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The Ubiquitin Code by Manuel S Rodriguez,Rosa Barrio Pdf

This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field.

Author : Angel L. Pey
Publisher : Academic Press
Page : 450 pages
File Size : 52,7 Mb
Release : 2020-02-13
Category : Science
ISBN : 9780128191330

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Protein Homeostasis Diseases by Angel L. Pey Pdf

Protein Homeostasis Diseases: Mechanisms and Novel Therapies offers an interdisciplinary examination of the fundamental aspects, biochemistry and molecular biology of protein homeostasis disease, including the use of natural and pharmacological small molecules to treat common and rare protein homeostasis disorders. Contributions from international experts discuss the biochemical and genetic components of protein homeostasis disorders, the mechanisms by which genetic variants may cause loss-of-function and gain-of-toxic-function, and how natural ligands can restore protein function and homeostasis in genetic diseases. Applied chapters provide guidance on employing high throughput sequencing and screening methodologies to develop pharmacological chaperones and repurpose approved drugs to treat protein homeostasis disorders. Provides an interdisciplinary examination of protein homeostasis disorders, with an emphasis on treatment strategies employing small natural and pharmacological ligands Offers applied approaches in employing high throughput sequencing and screening to develop pharmacological chaperones to treat protein homeostasis disease Gathers expertise from a range of international chapter authors who work across various biological methods and disease specific disciplines of relevance

Author : Anirban Bhunia,Hanudatta S Atreya,Neeraj Sinha
Publisher : Royal Society of Chemistry
Page : 733 pages
File Size : 46,9 Mb
Release : 2022-08-17
Category : Science
ISBN : 9781839165719

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NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces by Anirban Bhunia,Hanudatta S Atreya,Neeraj Sinha Pdf

NMR spectroscopy has found a wide range of applications in life sciences over recent decades. Providing a comprehensive amalgamation of the scattered knowledge of how to apply high-resolution NMR techniques to biomolecular systems, this book will break down the conventional stereotypes in the use of NMR for structural studies. The major focus is on novel approaches in NMR which deal with the functional interface of either protein-protein interactions or protein-lipid interactions. Bridging the gaps between structural and functional studies, the Editors believe a thorough compilation of these studies will open an entirely new dimension of understanding of crucial functional motifs. This in turn will be helpful for future applications into drug design or better understanding of systems. The book will appeal to NMR practitioners in industry and academia who are looking for a comprehensive understanding of the possibilities of applying high-resolution NMR spectroscopic techniques in probing biomolecular interactions.

Author : Andres F. Oberhauser
Publisher : Springer Science & Business Media
Page : 274 pages
File Size : 50,6 Mb
Release : 2012-11-09
Category : Science
ISBN : 9781461449218

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Single-molecule Studies of Proteins by Andres F. Oberhauser Pdf

In Single Molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action. The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.

Author : Anonim
Publisher : Elsevier
Page : 4609 pages
File Size : 55,5 Mb
Release : 2017-06-03
Category : Technology & Engineering
ISBN : 9780128032015

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Comprehensive Medicinal Chemistry III by Anonim Pdf

Comprehensive Medicinal Chemistry III, Eight Volume Set provides a contemporary and forward-looking critical analysis and summary of recent developments, emerging trends, and recently identified new areas where medicinal chemistry is having an impact. The discipline of medicinal chemistry continues to evolve as it adapts to new opportunities and strives to solve new challenges. These include drug targeting, biomolecular therapeutics, development of chemical biology tools, data collection and analysis, in silico models as predictors for biological properties, identification and validation of new targets, approaches to quantify target engagement, new methods for synthesis of drug candidates such as green chemistry, development of novel scaffolds for drug discovery, and the role of regulatory agencies in drug discovery. Reviews the strategies, technologies, principles, and applications of modern medicinal chemistry Provides a global and current perspective of today's drug discovery process and discusses the major therapeutic classes and targets Includes a unique collection of case studies and personal assays reviewing the discovery and development of key drugs

Author : Marcus Groettrup
Publisher : Springer Science & Business Media
Page : 270 pages
File Size : 48,6 Mb
Release : 2011-01-11
Category : Science
ISBN : 9781441966766

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Conjugation and Deconjugation of Ubiquitin Family Modifiers by Marcus Groettrup Pdf

† 1 a a 4 † 17 10 15 ubiquitin; and of 16 VCP 17 18 20 33 34 34 36 p domain. 41 42 42 43 P U 42 47 binding. C. elegans 16 In 21 22 50 51 52 53 13 and UFD 4 10 of Cdc48. 18 30 of Ufd2. COFACTORS 47 23 13 47 47 47 72 15 15 and of Spt23 p90. Ufd2 and Cdc48. In C. elegans 74 16 75 75 76 76 Ufd2 25 54 54 7 56 p47 7 7 80 30 30 81 82 82 but and CD3 26 DUB COFACTORS 30 UFD3 OTU1 4 Cdc48 30 4 OLE1. 15 27 87 REFERENCES 30 REGULATION OF UBIQUITIN MONOUBIQUITINATION UBIQUITINATION 1 32 7 S) d 33 12 13 14 15 18 19 15 20 21 35 15 15 27 15 31 32 31 33 36 monoubiquitination of pol pol 34 37 34 monoubiquitination. 20 35 trans 3 15 REFERENCES by monoubiquitination. Mol Cell; 2009. UBIQUITIN LIGASE ACTIVITY BY Nedd 1 2 of 41 5 6 8 fold. 9 13 14 edd 43 18 18 K M and k 18 22 23 K M 24 25 K M 26 edd 45 18 27 K M K D 18 25 . 8 10 M 21 28 MECHANISM AND REGULATION OF CRLs 34 41 34 edd 47 48 S. pombe 49 51 p27 and I by SCF and SCF 57 58 59 60 CTD CTD CTD CTD in Cul5 CTD CTD CTD 60 18