Single Molecule Studies Of Proteins

Author : David Bensimon,Vincent Croquette,Jean-François Allemand,Xavier Michalet,Terence Strick
Publisher : Oxford University Press
Page : 224 pages
File Size : 53,9 Mb
Release : 2018-11-30
Category : Science
ISBN : 9780192559968

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Single-Molecule Studies of Nucleic Acids and Their Proteins by David Bensimon,Vincent Croquette,Jean-François Allemand,Xavier Michalet,Terence Strick Pdf

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Author : Andres F. Oberhauser
Publisher : Springer Science & Business Media
Page : 274 pages
File Size : 49,7 Mb
Release : 2012-11-09
Category : Science
ISBN : 9781461449218

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Single-molecule Studies of Proteins by Andres F. Oberhauser Pdf

In Single Molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action. The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.

Author : Alexander E. Knight
Publisher : Academic Press
Page : 368 pages
File Size : 46,6 Mb
Release : 2009-02-26
Category : Science
ISBN : 008092123X

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Single Molecule Biology by Alexander E. Knight Pdf

Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective. The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. * Organized by biological system or molecule. * Each chapter discusses insights into mechanism of action, structure, and function * Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc. * Includes an introduction to key principles and an historical overview. * Discusses applications in molecular diagnostics and drug discovery. * Provides an expert's perspective on future developments.

Author : Krishnarao Appasani,Raghu Kiran Appasani
Publisher : Unknown
Page : 172 pages
File Size : 49,8 Mb
Release : 2022-05-11
Category : Medical
ISBN : 9781108534499

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Single-Molecule Science by Krishnarao Appasani,Raghu Kiran Appasani Pdf

A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Author : BENSIMON ET AL.
Publisher : Unknown
Page : 128 pages
File Size : 42,6 Mb
Release : 2024-04-28
Category : Electronic
ISBN : 0191864714

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SINGLE MOLECULE STUDIES OF NUCLEIC ACIDS AND THEIR PROTEINS. by BENSIMON ET AL. Pdf

Author : Peter Hinterdorfer,Antoine van Oijen
Publisher : Springer Science & Business Media
Page : 634 pages
File Size : 49,9 Mb
Release : 2009-12-24
Category : Science
ISBN : 9780387764979

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Handbook of Single-Molecule Biophysics by Peter Hinterdorfer,Antoine van Oijen Pdf

This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Author : Jane Clarke,Robert Best,Alessandro Borgia
Publisher : Wiley
Page : 300 pages
File Size : 44,5 Mb
Release : 2014-09-02
Category : Science
ISBN : 0470889489

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Single Molecule Studies of Protein Folding by Jane Clarke,Robert Best,Alessandro Borgia Pdf

The first comprehensive book to focus on the subject, Single Molecule Studies of Protein Folding deftly examines the two broad themes of the two principle single molecule methodologies used to study protein folding: force microscopy (AFM and optical / magnetic tweezers) and fluorescence resonance energy transfer (FRET), which is widely used to "measure" dimensions either within or between molecules over distances of 10-100A. Featuring sections written by preeminent scientists in the field, the text includes theoretical and simulation chapters as well as experimental work.

Author : Iddo Heller,David Dulin,Erwin J.G. Peterman
Publisher : Humana
Page : 0 pages
File Size : 50,7 Mb
Release : 2023-11-09
Category : Science
ISBN : 1071633767

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Single Molecule Analysis by Iddo Heller,David Dulin,Erwin J.G. Peterman Pdf

This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 53,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Krishnarao Appasani,Raghu Kiran Appasani
Publisher : Cambridge University Press
Page : 171 pages
File Size : 42,9 Mb
Release : 2022-05-26
Category : Medical
ISBN : 9781108423366

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Single-Molecule Science by Krishnarao Appasani,Raghu Kiran Appasani Pdf

A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Author : Toshio Yanagida,Yoshiharu Ishii
Publisher : John Wiley & Sons
Page : 346 pages
File Size : 40,9 Mb
Release : 2008-12-04
Category : Science
ISBN : 9783527626144

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Single Molecule Dynamics in Life Science by Toshio Yanagida,Yoshiharu Ishii Pdf

In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Author : Erwin J. G. Peterman,Gijs J. L. Wuite
Publisher : Humana Press
Page : 317 pages
File Size : 44,5 Mb
Release : 2011-09-15
Category : Science
ISBN : 1617792810

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Single Molecule Analysis by Erwin J. G. Peterman,Gijs J. L. Wuite Pdf

Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Author : Ke-li Han,Xin Zhang,Ming-jun Yang
Publisher : Springer Science & Business Media
Page : 488 pages
File Size : 50,7 Mb
Release : 2014-01-20
Category : Medical
ISBN : 9783319029702

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Protein Conformational Dynamics by Ke-li Han,Xin Zhang,Ming-jun Yang Pdf

This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.

Author : Daniel Cibrán Pérez González
Publisher : Unknown
Page : 194 pages
File Size : 46,6 Mb
Release : 2017
Category : DNA-protein interactions
ISBN : OCLC:1014216529

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Single-molecule Studies of Nucleic Acid Folding and Nucleic Acid-protein Interactions by Daniel Cibrán Pérez González Pdf

Author : Sharona Cohen
Publisher : Springer
Page : 59 pages
File Size : 44,7 Mb
Release : 2015-10-17
Category : Science
ISBN : 9783319095585

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Single-Molecule Fluorescence Spectroscopy of the Folding of a Repeat Protein by Sharona Cohen Pdf

In this thesis single-molecule fluorescence resonance energy transfer (FRET) spectroscopy was used to study the folding of a protein that belongs to the large and important family of repeat proteins. Cohen shows that the dynamics of the expanded conformations is likely to be very fast, suggesting a spring-like motion of the whole chain. The findings shed new light on the elasticity of structure in repeat proteins, which is related to their function in binding multiple and disparate partners. This concise research summary provides useful insights for students beginning a PhD in this or a related area, and researchers entering this field.