Protein Engineering And Design

Author : Paul R. Carey
Publisher : Elsevier
Page : 379 pages
File Size : 54,5 Mb
Release : 1996-06-21
Category : Science
ISBN : 9780080539973

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Protein Engineering and Design by Paul R. Carey Pdf

The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. Introduces and defines the techniques involved in protein engineering and design Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides

Author : Sheldon J. Park,Jennifer R. Cochran
Publisher : CRC Press
Page : 434 pages
File Size : 50,8 Mb
Release : 2009-09-25
Category : Medical
ISBN : 9781420076592

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Protein Engineering and Design by Sheldon J. Park,Jennifer R. Cochran Pdf

Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science

Author : Sheldon J. Park,Jennifer R. Cochran
Publisher : Unknown
Page : 402 pages
File Size : 55,6 Mb
Release : 2010
Category : Protein engineering
ISBN : 0429191634

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Protein Engineering and Design by Sheldon J. Park,Jennifer R. Cochran Pdf

Author : Tuck Seng Wong,Kang Lan Tee
Publisher : Springer Nature
Page : 213 pages
File Size : 45,7 Mb
Release : 2020-10-29
Category : Science
ISBN : 9783030568986

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A Practical Guide to Protein Engineering by Tuck Seng Wong,Kang Lan Tee Pdf

This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

Author : Huimin Zhao
Publisher : John Wiley & Sons
Page : 41 pages
File Size : 47,9 Mb
Release : 2021-08-23
Category : Science
ISBN : 9783527344703

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Protein Engineering by Huimin Zhao Pdf

A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.

Author : Mallorie N. Sheehan
Publisher : Nova Science Publishers
Page : 0 pages
File Size : 41,5 Mb
Release : 2013-07
Category : Protein engineering
ISBN : 1626188785

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Protein Engineering by Mallorie N. Sheehan Pdf

Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin.

Author : Ilan Samish
Publisher : Humana
Page : 0 pages
File Size : 42,8 Mb
Release : 2016-12-03
Category : Science
ISBN : 1493966359

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Computational Protein Design by Ilan Samish Pdf

The aim this volume is to present the methods, challenges, software, and applications of this widespread and yet still evolving and maturing field. Computational Protein Design, the first book with this title, guides readers through computational protein design approaches, software and tailored solutions to specific case-study targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Protein Design aims to ensure successful results in the further study of this vital field.

Author : Paul Wrede,Gisbert Schneider
Publisher : de Gruyter
Page : 404 pages
File Size : 54,7 Mb
Release : 1994
Category : Business & Economics
ISBN : UOM:39015033328595

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Concepts in Protein Engineering and Design by Paul Wrede,Gisbert Schneider Pdf

No detailed description available for "Concepts in Protein Engineering and Design".

Author : Yury E. Khudyakov
Publisher : CRC Press
Page : 588 pages
File Size : 49,9 Mb
Release : 2008-12-01
Category : Medical
ISBN : 9781420007305

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Medicinal Protein Engineering by Yury E. Khudyakov Pdf

An All-Inclusive Review of the Achievements and Trends in the Fast-Growing Protein Engineering Field From humble beginnings like making fire for mere survival, engineering now steadfastly penetrates all aspects of our lives and even life itself at the molecular level. Protein engineering is a molecular biological discipline focused on designing and

Author : Nediljko Budisa
Publisher : John Wiley & Sons
Page : 312 pages
File Size : 44,7 Mb
Release : 2006-05-12
Category : Science
ISBN : 9783527607099

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Engineering the Genetic Code by Nediljko Budisa Pdf

The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.

Author : Uwe T. Bornscheuer,Matthias Höhne
Publisher : Humana Press
Page : 350 pages
File Size : 44,8 Mb
Release : 2017-10-30
Category : Science
ISBN : 1493973649

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Protein Engineering by Uwe T. Bornscheuer,Matthias Höhne Pdf

This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.

Author : Kristian Müller,Katja Arndt
Publisher : Springer Science & Business Media
Page : 318 pages
File Size : 49,5 Mb
Release : 2007-10-26
Category : Science
ISBN : 9781597451871

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Protein Engineering Protocols by Kristian Müller,Katja Arndt Pdf

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Author : Paul Wrede,Gisbert Schneider
Publisher : Walter de Gruyter GmbH & Co KG
Page : 396 pages
File Size : 52,7 Mb
Release : 2020-10-12
Category : Science
ISBN : 9783110874242

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Concepts in Protein Engineering and Design by Paul Wrede,Gisbert Schneider Pdf

No detailed description available for "Concepts in Protein Engineering and Design".

Author : Mallorie N. Sheehan
Publisher : Unknown
Page : 221 pages
File Size : 44,6 Mb
Release : 2013
Category : Protein engineering
ISBN : 1616686499

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Protein Engineering by Mallorie N. Sheehan Pdf

Author : Lilia Alberghina
Publisher : CRC Press
Page : 374 pages
File Size : 40,6 Mb
Release : 2003-09-02
Category : Medical
ISBN : 9780203303702

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Protein Engineering For Industrial Biotechnology by Lilia Alberghina Pdf

Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering. The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery. International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.