Protein Engineering Techniques

Author : Kristian Müller,Katja Arndt
Publisher : Springer Science & Business Media
Page : 318 pages
File Size : 52,6 Mb
Release : 2007-10-26
Category : Science
ISBN : 9781597451871

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Protein Engineering Protocols by Kristian Müller,Katja Arndt Pdf

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Author : Tuck Seng Wong,Kang Lan Tee
Publisher : Springer Nature
Page : 213 pages
File Size : 52,8 Mb
Release : 2020-10-29
Category : Science
ISBN : 9783030568986

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A Practical Guide to Protein Engineering by Tuck Seng Wong,Kang Lan Tee Pdf

This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

Author : Krishna Mohan Poluri,Khushboo Gulati
Publisher : Springer
Page : 134 pages
File Size : 50,8 Mb
Release : 2016-11-12
Category : Technology & Engineering
ISBN : 9789811027321

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Protein Engineering Techniques by Krishna Mohan Poluri,Khushboo Gulati Pdf

This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 45,9 Mb
Release : 1988
Category : Electronic
ISBN : OCLC:437052471

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Enzymes for Biotechnology by Anonim Pdf

Author : Paul R. Carey
Publisher : Elsevier
Page : 379 pages
File Size : 49,6 Mb
Release : 1996-06-21
Category : Science
ISBN : 9780080539973

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Protein Engineering and Design by Paul R. Carey Pdf

The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. Introduces and defines the techniques involved in protein engineering and design Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides

Author : Dpet
Publisher : Unknown
Page : 128 pages
File Size : 46,6 Mb
Release : 1989-06
Category : Genetic engineering
ISBN : 0471424102

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DNA and Protein Engineering Techniques by Dpet Pdf

Author : Uwe T. Bornscheuer,Matthias Höhne
Publisher : Humana Press
Page : 350 pages
File Size : 43,9 Mb
Release : 2017-10-30
Category : Science
ISBN : 1493973649

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Protein Engineering by Uwe T. Bornscheuer,Matthias Höhne Pdf

This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.

Author : Caroline Koehrer,Uttam L. RajBhandary
Publisher : Springer Science & Business Media
Page : 351 pages
File Size : 47,9 Mb
Release : 2009-01-07
Category : Science
ISBN : 9783540709411

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Protein Engineering by Caroline Koehrer,Uttam L. RajBhandary Pdf

Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of "Protein Engineering". The field of protein engineering has, however, evolved rapidly since then and the last fifteen years have witnessed remarkable advances through the use of new chemical, biochemical and molecular biological tools towards the synthesis and manipulation of proteins. The chapters included in this book reflect the rapid evolution of protein engineering and its many applications in basic research, biotechnology, material sciences and therapy. This book will provide the reader with an introduction to state-of the-art concepts and methods and will be of use to anyone interested in the study of proteins, in academia as well as in industry.

Author : Carmichael J.A. Wallace
Publisher : CRC Press
Page : 300 pages
File Size : 41,8 Mb
Release : 1999-09-29
Category : Medical
ISBN : 0849347270

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Protein Engineering by Semisynthesis by Carmichael J.A. Wallace Pdf

As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features

Author : Tomohisa Ogawa
Publisher : BoD – Books on Demand
Page : 198 pages
File Size : 53,6 Mb
Release : 2013-05-29
Category : Science
ISBN : 9789535111382

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Protein Engineering by Tomohisa Ogawa Pdf

Given the centrality of protein to many biological process, this book makes a significant contribution to the fields of healthcare and nutrition. Its chapters consider topics such as protein-protein and protein-ligand docking, and the protein engineering of enzymes involved in bioplastic metabolism. One contribution gives an overview of the In Vitro Virus (IVV) analytic method, while another shows how cutting-edge techniques in protein engineering advance our knowledge in the field of palaeontology. The book also includes a review of classic and alternative strategies when using yeasts in research, with a focus on Pichia pastoris as a host. Finally, there are two contributions on chromatography: one on the method itself, and another on its use to identify HMGB1-binding components.

Author : Paul Wrede,Gisbert Schneider
Publisher : Walter de Gruyter GmbH & Co KG
Page : 396 pages
File Size : 46,6 Mb
Release : 2020-10-12
Category : Science
ISBN : 9783110874242

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Concepts in Protein Engineering and Design by Paul Wrede,Gisbert Schneider Pdf

No detailed description available for "Concepts in Protein Engineering and Design".

Author : Huimin Zhao
Publisher : John Wiley & Sons
Page : 41 pages
File Size : 50,8 Mb
Release : 2021-08-23
Category : Science
ISBN : 9783527344703

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Protein Engineering by Huimin Zhao Pdf

A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.

Author : Ernst-Ludwig Winnacker,Robert Huber
Publisher : Springer Science & Business Media
Page : 141 pages
File Size : 55,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642741739

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Protein Structure and Protein Engineering by Ernst-Ludwig Winnacker,Robert Huber Pdf

Protein engineering has had considerable impact on basic and applied research in biochemistry and molecular biology. It is already in use as a tool in molecular biology, but it is beginning to strongly influence the planning of experiments in biology everywhere, and, with even fur ther-reaching consequences, the appointment politics in research in stitutions and industries. Protein engineering, perhaps more than any other methods of protein analysis and peptide synthesis, has shown that proteins are organic molecules governed by the universal laws of chemistry and physics. How ever, as was the case with other new powerful methods and techniques, protein engineering tempts to an exploration of its limitations and thus generates more questions than it answers. The 39th Mosbacher Colloquium on Protein Structure and Protein Engi neering is not the first conference on this topic and it will not be the last. The important issues are obviously techniques of protein en gineering, examples of application, and the basic framework of protein structure and stability within which reasonable experiments can be de signed; conversely also, what we can learn about protein structure, dynamics, and folding from such experiments. Experiments in this direc tion aim at elucidating the folding code in the long run, but help to exploit the role of individual amino acid residues in catalysis, pro tein stability, and binding specificity in selected proteins now.

Author : Jeffrey L. Cleland,Charles S. Craik
Publisher : Wiley-Liss
Page : 558 pages
File Size : 48,9 Mb
Release : 1996-02-07
Category : Science
ISBN : UOM:39015037309690

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Protein Engineering by Jeffrey L. Cleland,Charles S. Craik Pdf

PROTEIN ENGINEERING Principles and Practice Edited by JEFFREY L. CLELAND CHARLES S. CRAIK Proteins are involved in every aspect of life-structure, motion, catalysis, recognition and regulation. Protein Engineering: Principles and Practice provides a basic framework for understanding both proteins and protein engineering. This comprehensive book covers general, yet essential knowledge required for successful protein engineering, including everything from the fundamentals to modifying existing proteins and developing new proteins. The book begins by introducing the main concepts of protein engineering, including: understanding protein conformation, comprehending the relationship between protein composition and structure, and potential methods for predicting a protein's conformation. Other major subjects addressed are: * Using different host cell expression systems to produce specific proteins * Protein folding * Structure and function of proteins in relation to drug design * Construction of synthetic metal binding sites in proteins * Manufacture of tissue plasminogen activator * Generation of therapeutic antibodies This broad range of topics provides a solid foundation in protein engineering and supplies readers with knowledge essential to the design and production of proteins. Of primary interest to protein scientists-both students and researchers, in academia as well as industry-Protein Engineering is also extremely useful to chemical engineers, protein chemists, biochemists, and pharmaceutical chemists.

Author : Sheldon J. Park,Jennifer R. Cochran
Publisher : CRC Press
Page : 434 pages
File Size : 52,9 Mb
Release : 2009-09-25
Category : Medical
ISBN : 9781420076592

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Protein Engineering and Design by Sheldon J. Park,Jennifer R. Cochran Pdf

Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science