Protocols For Adult Stem Cells

Author : Irina M. Conboy,David V. Schaffer,Mary Helen Barcellos-Hoff,Song Li
Publisher : Methods in Molecular Biology
Page : 228 pages
File Size : 51,9 Mb
Release : 2010-04-22
Category : Medical
ISBN : STANFORD:36105211741322

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Protocols for Adult Stem Cells by Irina M. Conboy,David V. Schaffer,Mary Helen Barcellos-Hoff,Song Li Pdf

The study of adult stem cells has surged in recent years. Because they are responsible for the body’s natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Critical and cutting edge, Protocols for Adult Stem Cells is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology.

Author : Paolo Di Nardo,Sanjiv Dhingra,Dinender K. Singla
Publisher : Humana
Page : 0 pages
File Size : 54,8 Mb
Release : 2017-02-25
Category : Science
ISBN : 1493967541

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Adult Stem Cells by Paolo Di Nardo,Sanjiv Dhingra,Dinender K. Singla Pdf

This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments.

Author : Ivan N. Rich
Publisher : Humana Press
Page : 0 pages
File Size : 45,8 Mb
Release : 2014-11-11
Category : Science
ISBN : 1493917846

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Stem Cell Protocols by Ivan N. Rich Pdf

This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.

Author : Andras Nagy,Kursad Turksen
Publisher : Springer Nature
Page : 829 pages
File Size : 48,8 Mb
Release : 2022-06-22
Category : Science
ISBN : 9781071621196

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Induced Pluripotent Stem (iPS) Cells by Andras Nagy,Kursad Turksen Pdf

This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.

Author : Gustav Steinhoff
Publisher : Springer Science & Business Media
Page : 1220 pages
File Size : 53,7 Mb
Release : 2013-03-20
Category : Medical
ISBN : 9789400756908

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Regenerative Medicine by Gustav Steinhoff Pdf

The field of regenerative medicine has developed rapidly over the past 20 years with the advent of molecular and cellular techniques. This textbook, Regenerative Medicine: From Protocol to Patient, aims to explain the scientific knowledge and emerging technology as well as the clinical application in different organ systems and diseases. International leading experts from four continents describe the latest scientific and clinical knowledge of the field of regenerative medicine. The process of translating science of laboratory protocols into therapies is explained in sections on regulatory, ethical and industrial issues. This textbook is organized into five parts: (I) Biology of Tissue Regeneration, (II) Stem Cell Science and Technology, (III) Tissue Engineering, Biomaterials and Nanotechnology, (IV) Regenerative Therapies and (V) Regulation and Ethics. The textbook aims to give the student, the researcher, the health care professional, the physician and the patient a complete survey on the current scientific basis, therapeutical protocols, clinical translation and practiced therapies in regenerative medicine.

Author : Peggy Stock,Bruno Christ
Publisher : Humana
Page : 284 pages
File Size : 47,8 Mb
Release : 2021-05-12
Category : Science
ISBN : 1071612247

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In Vitro Models for Stem Cell Therapy by Peggy Stock,Bruno Christ Pdf

This volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field

Author : Shree Ram Singh,Pranela Rameshwar
Publisher : Humana Press
Page : 284 pages
File Size : 44,8 Mb
Release : 2018-09-09
Category : Science
ISBN : 1493986961

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Somatic Stem Cells by Shree Ram Singh,Pranela Rameshwar Pdf

This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.

Author : R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach
Publisher : John Wiley & Sons
Page : 360 pages
File Size : 55,9 Mb
Release : 2007-07-16
Category : Science
ISBN : 0470167513

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Culture of Human Stem Cells by R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach Pdf

This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Author : Tanja Zigova,Paul R. Sanberg,Juan R. Sanchez-Ramos
Publisher : Springer Science & Business Media
Page : 373 pages
File Size : 40,8 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592591862

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Neural Stem Cells by Tanja Zigova,Paul R. Sanberg,Juan R. Sanchez-Ramos Pdf

Over the last decade, neural stem cell research has provided penetrating insights into the plasticity and regenerative potential of the brain. Stem cells have been isolated from embryonic as well as adult central nervous system (CNS). Many non-CNS mammalian tissues also contain stem cells with a more limited repertoire: the replacement of tissue-specific cells throughout the li- time of the organism. Progress has been made in understanding fundamental stem cell properties that depend on the interplay of extrinsic signaling factors with intrinsic genetic programs within critical time frames. With this growing knowledge, scientists have been able to change a neural stem cell’s fate. - der certain conditions, neural stem cells have been induced to differentiate into cells outside the expected neural lineage and conversely, stem cells from nonneural tissue have been shown to transdifferentiate into cells with distinct neural phenotypes. At the moment, there is an accelerated effort to identify a readily ava- able, socially acceptable stem cell that can be induced to proliferate in an und- ferentiated state and that can be manipulated at will to generate diverse cells types. We are on the threshold of a great new therapeutic era of cellular therapy that has as great, if not greater, potential as the current pharmacologic era, g- rified by antibiotics, anesthetics, pain killers, immunosuppressants, and psyc- tropics.

Author : National Research Council,Institute of Medicine,Board on Health Sciences Policy,Division on Earth and Life Studies,Board on Life Sciences,Committee on Guidelines for Human Embryonic Stem Cell Research
Publisher : National Academies Press
Page : 224 pages
File Size : 46,7 Mb
Release : 2005-09-15
Category : Medical
ISBN : 9780309096539

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Guidelines for Human Embryonic Stem Cell Research by National Research Council,Institute of Medicine,Board on Health Sciences Policy,Division on Earth and Life Studies,Board on Life Sciences,Committee on Guidelines for Human Embryonic Stem Cell Research Pdf

Since 1998, the volume of research being conducted using human embryonic stem (hES) cells has expanded primarily using private funds because of restrictions on the use of federal funds for such research. Given limited federal involvement, privately funded hES cell research has thus far been carried out under a patchwork of existing regulations, many of which were not designed with this research specifically in mind. In addition, hES cell research touches on many ethical, legal, scientific, and policy issues that are of concern to the public. This report provides guidelines for the conduct of hES cell research to address both ethical and scientific concerns. The guidelines are intended to enhance the integrity of privately funded hES cell research by encouraging responsible practices in the conduct of that research.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 53,8 Mb
Release : 2024-06-30
Category : Stem cells
ISBN : 0470151803

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Current Protocols in Stem Cell Biology by Anonim Pdf

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 42,7 Mb
Release : 2024-06-30
Category : Stem cells
ISBN : 0470151803

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Current Protocols in Stem Cell Biology by Anonim Pdf

Author : Craig Atwood
Publisher : BoD – Books on Demand
Page : 416 pages
File Size : 47,8 Mb
Release : 2011-04-26
Category : Science
ISBN : 9789533071985

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Embryonic Stem Cells by Craig Atwood Pdf

Pluripotent stem cells have the potential to revolutionise medicine, providing treatment options for a wide range of diseases and conditions that currently lack therapies or cures. This book describes recent advances in the generation of tissue specific cell types for regenerative applications, as well as the obstacles that need to be overcome in order to recognize the potential of these cells.

Author : Anonim
Publisher : Elsevier
Page : 516 pages
File Size : 48,6 Mb
Release : 2006-12-12
Category : Technology & Engineering
ISBN : 9780080469645

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Stem Cell Tools and Other Experimental Protocols by Anonim Pdf

This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering Presents the latest most innovative technologies Addresses therapeutic relevance including FDA compliance and tissue engineering

Author : KURSAD TURKSEN
Publisher : Springer Science & Business Media
Page : 390 pages
File Size : 52,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450461

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Human Embryonic Stem Cell Protocols by KURSAD TURKSEN Pdf

A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.