Regulation Of Alternative Splicing

Author : Philippe Jeanteur
Publisher : Springer Science & Business Media
Page : 254 pages
File Size : 54,8 Mb
Release : 2013-03-14
Category : Science
ISBN : 9783662097281

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Regulation of Alternative Splicing by Philippe Jeanteur Pdf

The discovery in 1977 that genes are split into exons and introns has done away with the one gene - one protein dogma. Indeed, the removal of introns from the primary RNA transcript is not necessarily straightforward since there may be optional pathways leading to different messenger RNAs and consequently to different proteins. Examples of such an alternative splicing mechanism cover all fields of biology. Moreover, there are plenty of occurrences where deviant splicing can have pathological effects. Despite the high number of specific cases of alternative splicing, it was not until recently that the generality and extent of this phenomenon was fully appreciated. A superficial reading of the preliminary sequence of the human genome published in 2001 led to the surprising, and even deceiving to many scientists, low number of genes (around 32,000) which contrasted with the much higher figure around 150,000 which was previously envisioned. Attempts to make a global assessment of the use of alternative splicing are recent and rely essentially on the comparison of genomic mRNA and EST sequences as reviewed by Thanaraj and Stamm in the first chapter of this volume. Most recent estimates suggest that 40-60% of human genes might be alternatively spliced, as opposed to about 22% for C. elegans.

Author : Benjamin J. Blencowe,Brenton R. Graveley
Publisher : Springer Science & Business Media
Page : 260 pages
File Size : 50,6 Mb
Release : 2008-01-23
Category : Medical
ISBN : 0387773738

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Alternative Splicing in the Postgenomic Era by Benjamin J. Blencowe,Brenton R. Graveley Pdf

Yet again Springer has reached the market before everyone. This is the first book that is solely dedicated to the topic of alternative splicing. The book contains chapters by experts in the field that cover nearly all aspects of this hugely important subject. The purpose of the text is to provide a single, authoritative source of information on alternative splicing that is accessible to researchers in diverse fields. It is suitable for beginners and experts alike.

Author : Philippe Jeanteur
Publisher : Unknown
Page : 266 pages
File Size : 42,9 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 366209729X

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Regulation of Alternative Splicing by Philippe Jeanteur Pdf

Author : Stefan Stamm,Chris Smith,Reinhard Lührmann
Publisher : John Wiley & Sons
Page : 660 pages
File Size : 55,5 Mb
Release : 2012-08-01
Category : Science
ISBN : 9783527647989

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Alternative pre-mRNA Splicing by Stefan Stamm,Chris Smith,Reinhard Lührmann Pdf

This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing. A theoretical introduction and practical guide for molecular biologists, geneticists,clinicians and every researcher interested in alternative splicing. Website: www.wiley-vch.de/home/splicing

Author : A. S. N. Reddy,Maxim V. Golovkin
Publisher : Springer Science & Business Media
Page : 323 pages
File Size : 45,5 Mb
Release : 2008-04-16
Category : Science
ISBN : 9783540767763

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Nuclear pre-mRNA Processing in Plants by A. S. N. Reddy,Maxim V. Golovkin Pdf

During the last few years, tremendous progress has been made in understanding various aspects of pre-mRNA processing. This book, with contributions from leading scientists in this area, summarizes recent advances in nuclear pre-mRNA processing in plants. It provides researchers in the field, as well as those in related areas, with an up-to-date and comprehensive, yet concise, overview of the current status and future potential of this research in understanding plant biology.

Author : Adrian Krainer
Publisher : IRL Press
Page : 408 pages
File Size : 55,6 Mb
Release : 1997
Category : Language Arts & Disciplines
ISBN : STANFORD:36105020129602

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Eukaryotic MRNA Processing by Adrian Krainer Pdf

This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.

Author : Anonim
Publisher : Academic Press
Page : 384 pages
File Size : 40,7 Mb
Release : 2016-05-27
Category : Medical
ISBN : 9780128024881

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Visualizing RNA Dynamics in the Cell by Anonim Pdf

Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in visualizing RNA dynamics in the cell Contains sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2 and visualization of 5S dynamics in live cells using photostable corn probe

Author : Ezequiel Petrillo,Maria Kalyna,Craig G. Simpson,Shih-Long Tu,Kranthi Kiran Mandadi
Publisher : Frontiers Media SA
Page : 175 pages
File Size : 43,8 Mb
Release : 2020-09-02
Category : Nature
ISBN : 9782889639748

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Alternative Splicing Regulation in Plants by Ezequiel Petrillo,Maria Kalyna,Craig G. Simpson,Shih-Long Tu,Kranthi Kiran Mandadi Pdf

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Author : Peter Gunning
Publisher : Springer Science & Business Media
Page : 323 pages
File Size : 47,5 Mb
Release : 2009-09-30
Category : Medical
ISBN : 9780387857664

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Tropomyosin by Peter Gunning Pdf

A recent review of one of my grant applications commented on the ‘rediscovery of tropomyosin’. I was tempted to write back in my rebuttal to the reviewer that I didn’t realise it had been lost. Uncharacteristic maturity prevailed and I resisted the temptation, but I was struck by the underlying observation that research on the str- ture and function of tropomyosin has been somewhat invisible, particularly in terms of the cytoskeleton isoforms. So, how can it be that one of the two major components of the actin filament has been so thoroughly overlooked? I suspect that the answer is disappointingly pedestrian. Whereas the biochemistry of the 1980s revealed the potential of tropomyosin isoforms to diversify the function of actin filaments, the subsequent disenchantment with isoform biology in general in the 1990s inhibited growth of this field. With the development of more sophisticated experimental - proaches we are now seeing a growing realisation of the importance of tropomyosin in regulating actin filaments beyond its pivotal role in muscle contraction. The opportunity to edit this book came at a time when we had written several reviews on different aspects of tropomyosin function and I had just finished the background reading for a comprehensive review of tropomyosin biology. I realised that the field was simply beyond the capacity of any one person to do the field j- tice.

Author : Anonim
Publisher : Academic Press
Page : 365 pages
File Size : 44,9 Mb
Release : 2014-08-27
Category : Science
ISBN : 9780128013199

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Brain Transcriptome by Anonim Pdf

Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. This volume, concentrates on the brain transcriptome. Brings together cutting-edge research on the brain transcriptome

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 49,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Philippe Jeanteur
Publisher : Springer Science & Business Media
Page : 265 pages
File Size : 47,9 Mb
Release : 2006-10-04
Category : Science
ISBN : 9783540344490

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Alternative Splicing and Disease by Philippe Jeanteur Pdf

Splicing of primary RNA transcript is a quasi-systematic step of gene expression in higher organisms. This is the first book to highlight the medical implications, i.e. diseases, caused by alternative splicing. Alternative splicing not only vastly increases protein diversity but also offers numerous opportunities for aberrant splicing events with pathological consequences. The book also outlines possible targets for therapy.

Author : Peter Scheiffele,Oriane Mauger
Publisher : Springer Nature
Page : 356 pages
File Size : 48,6 Mb
Release : 2022-07-27
Category : Science
ISBN : 9781071625217

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Alternative Splicing by Peter Scheiffele,Oriane Mauger Pdf

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Author : Christina Smolke
Publisher : John Wiley & Sons
Page : 1600 pages
File Size : 41,8 Mb
Release : 2018-02-28
Category : Science
ISBN : 9783527688098

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Synthetic Biology by Christina Smolke Pdf

A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.

Author : Kif Liakath-Ali,Matthias Soller
Publisher : Frontiers Media SA
Page : 171 pages
File Size : 50,7 Mb
Release : 2023-12-13
Category : Science
ISBN : 9782832540732

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Alternative Splicing in Brain Function by Kif Liakath-Ali,Matthias Soller Pdf

Functional and structural intricacies of biological systems emerge from the complex nature of the genome. A key aspect of genome complexity can be attributed to the process of alternative splicing of precursor mRNA (or pre-mRNA), during which introns are removed and exons are selectively spliced together. This highly regulated process generates different mature mRNA transcripts from a single gene and is widespread throughout the eukaryotic evolution. The majority of the genes expressed in the mammalian central nervous system undergo extensive alternative splicing. Some genes can generate more than a thousand isoforms resulting in diverse proteoforms that can differ in their function, binding preference, catalytic activity, and localization. Perturbation in alternative splicing has been linked to many neurological disorders. Despite its significance, we still lack a comprehensive understanding of its role in neuronal function. Recent studies suggest that alternative splicing is a regulator of tissue identity and development. This is particularly relevant to the brain where developmental regulation and heterogenous cell population play a crucial role in shaping its function. For a long time, technological limitations in detecting and measuring alternatively spliced transcripts hindered the progress in understanding the biology of alternative splicing. Recent advances in long-read and native mRNA sequencing and robust computational tools allow us to capture and quantify in their full-length form. Public transcriptomics datasets can be utilized for the meta-analysis of alternative splicing landscapes of various experimental conditions and disease samples. In addition, protein isoform detection complements mRNA isoform levels. Given its biological significance and recent advances, the objective of this Research Topic is to assemble current knowledge, views, and challenges in the studies of alternative splicing in brain function.