Ribozymes And Sirna Protocols

Ribozymes And Sirna Protocols Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Ribozymes And Sirna Protocols book. This book definitely worth reading, it is an incredibly well-written.

Ribozymes and siRNA protocols

Mouldy Sioud
Ribozymes and siRNA protocols Book PDF

Author : Mouldy Sioud
Publisher : Springer Science & Business Media
Page : 623 pages
File Size : 50,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597468

Get Book

Ribozymes and siRNA protocols by Mouldy Sioud Pdf

In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, hammerhead ribozymes and derivatives, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, RNase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozyme protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequence-specific gene silencing in a wide variety of organisms. Additional techniques cover the analysis of ribozyme structures and conformational transitions using nucleotide analog interference mapping and fluorescence resonance energy transfer, the use of ribozymes in clinical and gene therapy, and the use of ribozymes and DNAzymes in rodent models of human disease. Each proven protocol includes a background introduction outlining the principle behind the technique, step-by-step instructions, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Ribozymes and siRNA Protocols details for experienced and novice investigators alike the many exciting advances in our understanding of nucleic acid enzymes, as well as demonstrating how they may be used to analyze gene function and target validation, and to productively develop novel therapeutics for human diseases.

Ribozymes and siRNA protocols

Mouldy Sioud
Ribozymes and siRNA protocols Book PDF

Author : Mouldy Sioud
Publisher : Humana Press
Page : 624 pages
File Size : 44,7 Mb
Release : 2004-03-05
Category : Science
ISBN : 1588292266

Get Book

Ribozymes and siRNA protocols by Mouldy Sioud Pdf

In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpain ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, Rnase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozymes protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequencing-specific gene silencing in a wide variety of organisms. Comprehensive and up-to-date, Ribozymes and siRNA Protocols synthesizes for experienced and novice investigators alike the exciting advances in understanding nucleic acid enzymes and demonstrates how they may be used to analyze gene function and target validation, and to productively develop new therapeutics for human diseases.

Ribozyme Protocols

Philip C. Turner
Ribozyme Protocols Book PDF

Author : Philip C. Turner
Publisher : Humana
Page : 492 pages
File Size : 51,5 Mb
Release : 2013-09-20
Category : Science
ISBN : 1489942858

Get Book

Ribozyme Protocols by Philip C. Turner Pdf

The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use—^not only to those with some experience of ribozymes—^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field, I believe this book approaches that ideal and should help all readers perform meaningful experiments using ribozymes. To design ribozymes, one must consider whether the target site will be accessible; this task can be facilitated by using computer programs that pre dict the folding of the target RNA. Such programs are detailed in Chapters 2 and 3. If the chosen target is an RNA virus that can mutate rapidly, it makes sense to consider those parts of the genome that are least likely to change during viral replication. An example of how this can be done is described in Chapter 4. Although computer analysis may be a useful starting point to select tar get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select ing those that actually hybridize to and/or cleave the target; these methods are described in Chapters 5 and 6.

Target Discovery and Validation Reviews and Protocols

Mouldy Sioud
Target Discovery and Validation Reviews and Protocols Book PDF

Author : Mouldy Sioud
Publisher : Springer Science & Business Media
Page : 359 pages
File Size : 50,9 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781597452083

Get Book

Target Discovery and Validation Reviews and Protocols by Mouldy Sioud Pdf

These volumes review the most current methods for drug target discovery and validation. They explore how recent improvement in understanding the molecular mechanisms of human pathology is impacting drug target discovery in the laboratory and in real therapeutics, specifically for cancers and autoimmune disorders. This book provides a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application.

RNA Towards Medicine

Volker A. Erdmann,Jürgen Brosius,Jan Barciszewski
RNA Towards Medicine Book PDF

Author : Volker A. Erdmann,Jürgen Brosius,Jan Barciszewski
Publisher : Springer Science & Business Media
Page : 463 pages
File Size : 53,7 Mb
Release : 2006-02-04
Category : Science
ISBN : 9783540272625

Get Book

RNA Towards Medicine by Volker A. Erdmann,Jürgen Brosius,Jan Barciszewski Pdf

Developments over the past few years have revealed the remarkable versatility of RNA in any compartment of the cell, tasks that had been thought to be exclusively in the realm of proteins and even beyond. The chapters in this book written by leading investigators in the field provide insight into various promising avenues where RNA and nucleic acid derivatives including antisense RNAs, such as siRNA, miRNAs, amplification/selection (SELEX) generated aptamers as well as ribozymes are at the threshold of impacting medicine.

Cytokine Protocols

Marc De Ley
Cytokine Protocols Book PDF

Author : Marc De Ley
Publisher : Springer Science & Business Media
Page : 248 pages
File Size : 47,5 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592596676

Get Book

Cytokine Protocols by Marc De Ley Pdf

A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Enjoy a quick and smooth introduction to the key methods used in cytokine research Use readily reproducible techniques that ensure successful experimental results Employ antisense-RNA, RT-PCR of small amounts of mRNA, and in situ hybridization.

Drosophila Cytogenetics Protocols

Daryl S. Henderson
Drosophila Cytogenetics Protocols Book PDF

Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
Page : 467 pages
File Size : 54,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596652

Get Book

Drosophila Cytogenetics Protocols by Daryl S. Henderson Pdf

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Cell Cycle Checkpoint Control Protocols

Howard B. Lieberman
Cell Cycle Checkpoint Control Protocols Book PDF

Author : Howard B. Lieberman
Publisher : Springer Science & Business Media
Page : 366 pages
File Size : 55,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596461

Get Book

Cell Cycle Checkpoint Control Protocols by Howard B. Lieberman Pdf

The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer.

Protein Purification Protocols

Paul Cutler
Protein Purification Protocols Book PDF

Author : Paul Cutler
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 45,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596553

Get Book

Protein Purification Protocols by Paul Cutler Pdf

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Small RNAs:

Wolfgang Nellen,Christian Hammann
Small RNAs Book PDF

Author : Wolfgang Nellen,Christian Hammann
Publisher : Springer Science & Business Media
Page : 220 pages
File Size : 42,9 Mb
Release : 2007-09-12
Category : Science
ISBN : 9783540281306

Get Book

Small RNAs: by Wolfgang Nellen,Christian Hammann Pdf

In recent years, the discovery of functional small RNAs has brought about an unprecedented revolution within the field of molecular biology. This volume describes strategies for the discovery and validation of small RNAs. It provides a snapshot of our current understanding of the different mechanisms triggered by small RNAs and the variations encountered in different organisms.

Cell Migration in Inflammation and Immunity

Daniele D’Ambrosio,Francesco Sinigaglia
Cell Migration in Inflammation and Immunity Book PDF

Author : Daniele D’Ambrosio,Francesco Sinigaglia
Publisher : Springer Science & Business Media
Page : 283 pages
File Size : 40,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592594351

Get Book

Cell Migration in Inflammation and Immunity by Daniele D’Ambrosio,Francesco Sinigaglia Pdf

Chemokines and their receptors play a central role in the pathogenesis of numerous, perhaps all, acute and chronic inflammatory diseases. About 50 distinct chemokines produced by a variety cell types and tissues either c- stitutively or in response to inflammatory stimuli are involved in a plethora of biological processes. These small secreted proteins exert their exquisitely variegated functions upon binding to a family of seven-transmembrane spanning G-protein coupled receptors (GPCRs) composed of almost 20 distinct entities. The biological activities of chemokines range from the control of leukocyte trafficking in basal and inflammatory conditions to the regulation of hema- poiesis, angiogenesis, tissue architecture, and organogenesis. The basis for such diversified activities rests, on one hand, upon the ubiquitous nature of chemokine production and chemokine receptor expression. Virtually every cell type can produce chemokines and expresses a unique combination of chemokine receptors. On the other hand, chemokine receptors make use of a flexible and complex network of intracellular signaling machineries that can regulate a variety of cellular functions ranging from cell migration, growth, and differentiation to death. As knowledge of the size of chemokine and chemokine receptor families rapidly reaches completeness, much is still to be uncovered in terms of fu- tional architecture of the chemokine system. The disparity between the large number of chemokines and that smaller number of receptors is balanced by the promiscuity in ligand–receptor interactions, with multiple chemokines binding to the same receptor and several chemokines binding to more than one receptor.

Mammalian Artificial Chromosomes

Vittorio Sgaramella,Sandro Eridani
Mammalian Artificial Chromosomes Book PDF

Author : Vittorio Sgaramella,Sandro Eridani
Publisher : Springer Science & Business Media
Page : 277 pages
File Size : 40,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592594344

Get Book

Mammalian Artificial Chromosomes by Vittorio Sgaramella,Sandro Eridani Pdf

In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has been slow but steady. Many scientists who took part in the workshop have since been engaged in active and prod- tive research. It goes to the credit of Humana Press to have realized the need for a book on artificial chromosomes that aims to provide better tools to all scientists committed to this field who are confronted with very difficult tech- cal problems. We have strived to cover in Mammalian Artificial Chromosomes: Methods and Protocols all relevant areas of artificial chromosome research, from basic genetics to daring attempts to build new tools for genetic therapy. We are of course grateful to the authors who have accepted the task of describing the technical steps and pitfalls that can be encountered in their research. Rarely has a very delicate methodology been presented with such meticulous care. We have been helped in this enterprise by the excellent librarian of the LITA Institute in Segrate, Italy, Ms. Claudia Piergigli, whom we thank warmly. Ms.

Atomic Force Microscopy

Pier Carlo Braga,Davide Ricci
Atomic Force Microscopy Book PDF

Author : Pier Carlo Braga,Davide Ricci
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 43,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596478

Get Book

Atomic Force Microscopy by Pier Carlo Braga,Davide Ricci Pdf

The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).

Chiral Separations

Gerald Gübitz,Martin G. Schmid
Chiral Separations Book PDF

Author : Gerald Gübitz,Martin G. Schmid
Publisher : Springer Science & Business Media
Page : 427 pages
File Size : 40,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596485

Get Book

Chiral Separations by Gerald Gübitz,Martin G. Schmid Pdf

Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.

HPLC of Peptides and Proteins

Marie-Isabel Aguilar
HPLC of Peptides and Proteins Book PDF

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
Page : 411 pages
File Size : 43,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597420

Get Book

HPLC of Peptides and Proteins by Marie-Isabel Aguilar Pdf

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.