Rna Interference And Crispr Technologies

Author : Mouldy Sioud
Publisher : Unknown
Page : 486 pages
File Size : 47,6 Mb
Release : 2020
Category : Gene expression
ISBN : 107160290X

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RNA Interference and CRISPR Technologies by Mouldy Sioud Pdf

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. .

Author : Aditi Singh,Mohammad W. Khan
Publisher : BoD – Books on Demand
Page : 136 pages
File Size : 45,9 Mb
Release : 2019-05-29
Category : Medical
ISBN : 9781838806965

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Modulating Gene Expression by Aditi Singh,Mohammad W. Khan Pdf

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

Author : Mohammad W. Khan,Aditi Singh
Publisher : Unknown
Page : 134 pages
File Size : 52,5 Mb
Release : 2019
Category : Genetics
ISBN : 1838806970

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Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies by Mohammad W. Khan,Aditi Singh Pdf

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.

Author : Kamel A Abd-Elsalam,Ki-Taek Lim
Publisher : Elsevier
Page : 842 pages
File Size : 54,8 Mb
Release : 2021-02-27
Category : Technology & Engineering
ISBN : 9780128219119

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CRISPR and RNAi Systems by Kamel A Abd-Elsalam,Ki-Taek Lim Pdf

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

Author : Krishnarao Appasani
Publisher : Cambridge University Press
Page : 564 pages
File Size : 49,8 Mb
Release : 2005-01-17
Category : Science
ISBN : 1139442651

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RNA Interference Technology by Krishnarao Appasani Pdf

RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.

Author : Muhammad Sohail
Publisher : CRC Press
Page : 398 pages
File Size : 42,6 Mb
Release : 2004-08-27
Category : Medical
ISBN : 9780203489253

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Gene Silencing by RNA Interference by Muhammad Sohail Pdf

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r

Author : Dr. Tim Doran,Chris Helliwell
Publisher : CABI
Page : 269 pages
File Size : 55,9 Mb
Release : 2009
Category : Science
ISBN : 9781845934101

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RNA Interference by Dr. Tim Doran,Chris Helliwell Pdf

This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.

Author : Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet
Publisher : Frontiers Media SA
Page : 103 pages
File Size : 52,5 Mb
Release : 2021-07-28
Category : Science
ISBN : 9782889711000

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Advances and Challenges of RNAi Based Technologies for Plants by Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet Pdf

Author : Anonim
Publisher : Academic Press
Page : 200 pages
File Size : 46,7 Mb
Release : 2021-04-29
Category : Science
ISBN : 9780323852982

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Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B by Anonim Pdf

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas. Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for insect cell line, protozoans, zebrafish, drosophila, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology and many more

Author : Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh
Publisher : Springer Nature
Page : 428 pages
File Size : 47,6 Mb
Release : 2021-05-13
Category : Science
ISBN : 9783030649944

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RNA-Based Technologies for Functional Genomics in Plants by Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh Pdf

This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for genes. Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further development and applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.

Author : Prerna Pandey
Publisher : Delve Publishing
Page : 0 pages
File Size : 54,6 Mb
Release : 2017-11
Category : Gene silencing
ISBN : 177361147X

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RNAi Technologies by Prerna Pandey Pdf

RNA interference (RNAi), one of the most exciting discoveries of the last decade, is also known as Post-Transcriptional Gene Silencing (PTGS). It is a conserved host defense response via double-stranded RNA against both endogenous and foreign nucleic acids, and occurs at the levels of transcription, post-transcription and translation. RNA induced gene silencing has been used, with considerable success as a reverse genetics tool for the study of gene function. This technology has revolutionized the gene expression analysis studies because of the specificity and speed it offers. The small RNA molecules central to RNAi are microRNA (miRNA) and small interfering RNA (siRNA). These molecules bind to other RNAs specifically, such as messenger RNA (mRNA), to regulate their activities.In this book, we will discuss how gene silencing methodology has been successfully applied as therapeutic tool against metabolic disorders, cancer, and infectious diseases. This tool has also been successfully used to improve crops resistance against plant pathogens. In contrast to conventional knock-out mutagenesis techniques, the "gene-silencing" vectors, that allow the study of functions of genes whose complete knock-out would otherwise be lethal to the plant.

Author : R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
Publisher : CRC Press
Page : 0 pages
File Size : 42,8 Mb
Release : 2011-04-28
Category : Science
ISBN : 1578087163

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RNAi Technology by R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta Pdf

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA interference in microorganisms, plants, and animals, this book reviews the selective and robust effect of RNAi on gene expression. This process is essential both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest.

Author : David Ross Engelke
Publisher : Unknown
Page : 0 pages
File Size : 43,6 Mb
Release : 2003
Category : Gene silencing
ISBN : 0966402782

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RNA Interference (RNAi) by David Ross Engelke Pdf

This work focuses on practical approaches to and uses of a revolutionary technology called RNAi for RNA interference. A recently developed research tool, this process allows scientists to knock out or knock down the levels of a gene product, shut off targeted genes in an organism, and then monitor the results within that organism. Leading academic and biotechnology researchers in various fields that utilize this process introduce and explain a specific model system and use of RNA interference as well as necessary background information. Tools for the Arabidopsis, Caenrhabditis, and Drosophila model organism systems are offered, as is an overview of siRNA design and RNA transfection processes in mammalian cells. A discussion of chemical and enzymatic synthesis and preparation of siRNA in vitro is also included.

Author : Wiley
Publisher : John Wiley & Sons
Page : 214 pages
File Size : 53,8 Mb
Release : 2013-11-26
Category : Science
ISBN : 9781118829448

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RNA Interference (RNAi) by Wiley Pdf

The 2006 Nobel Prize in Physiology or Medicine was awarded to Andrew Z. Fire and Craig C. Mello for their discovery of RNA interference (RNAi). RNAi is a biological process in which RNA molecules can silence (inhibit) or up- or down-regulate gene expression, typically by causing the destruction of specific messenger RNA (mRNA) molecules. This collection of articles begins with an introduction to RNAi, and proceeds to describe uses of this technology in various approaches to disease treatment, including gene therapy. Several laboratory protocols for silencing genes via RNAi are also provided, as are protocols for down-regulating and then rescuing those down-regulated genes, which demonstrates specificity of the approach. This e-book — a curated collection from eLS, WIREs, and Current Protocols — offers a fantastic introduction to the field of RNA interference for students or interdisciplinary collaborators.

Author : Gregory J. Hannon
Publisher : Unknown
Page : 456 pages
File Size : 50,9 Mb
Release : 2003
Category : Gene silencing
ISBN : UOM:39015058068399

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RNAi by Gregory J. Hannon Pdf

In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.