Rna Protein Interaction Protocols

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RNA'Protein Interaction Protocols

Susan R. Haynes
RNA Protein Interaction Protocols Book PDF

Author : Susan R. Haynes
Publisher : Springer Science & Business Media
Page : 485 pages
File Size : 48,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596768

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RNA'Protein Interaction Protocols by Susan R. Haynes Pdf

The molecular characterization of RNA and its interactions with proteins is an important and exciting area of current research. Organisms utilize a variety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscriptional processes are critical components of gene regulation has sparked an explosion of interest in both stable ribonucleoprotein (RNP) complexes and transient RNA–protein interactions. RNA is conformationally flexible and can adopt complex structures that provide diverse surfaces for interactions with proteins. The fact that short RNA molecules (aptamers; see Chapter 16) can be selected to bind many different types of molecules is evidence of the structural variability of RNA. RNA molecules are rarely entirely single- or double-stranded, but usually contain multiple short duplexes interrupted by single-stranded loops and bulges; in some RNAs, such as tRNAs, the short duplexes stack on each other. Further variability is generated by the presence of non-Watson-Crick base pairs, modified nucleotides, and more complex structures, such as pseudoknots and triple-strand interactions.

RNA-Protein Complexes and Interactions

Ren-Jang Lin
RNA Protein Complexes and Interactions Book PDF

Author : Ren-Jang Lin
Publisher : Springer Nature
Page : 349 pages
File Size : 50,8 Mb
Release : 2023-05-11
Category : Science
ISBN : 9781071631911

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RNA-Protein Complexes and Interactions by Ren-Jang Lin Pdf

This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

RNA-Protein Interactions : A Practical Approach

Christopher W.J. Smith
RNA Protein Interactions A Practical Approach Book PDF

Author : Christopher W.J. Smith
Publisher : Oxford University Press, UK
Page : 370 pages
File Size : 41,5 Mb
Release : 1998-07-09
Category : Electronic
ISBN : 9780191591624

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RNA-Protein Interactions : A Practical Approach by Christopher W.J. Smith Pdf

RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -

RNA-protein Interactions

Kiyoshi Nagai,Iain W. Mattaj
RNA protein Interactions Book PDF

Author : Kiyoshi Nagai,Iain W. Mattaj
Publisher : Oxford University Press, USA
Page : 302 pages
File Size : 41,5 Mb
Release : 1994
Category : Medical
ISBN : UOM:39015031725248

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RNA-protein Interactions by Kiyoshi Nagai,Iain W. Mattaj Pdf

The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.

RNA-RNA Interactions

Frank J. Schmidt
RNA RNA Interactions Book PDF

Author : Frank J. Schmidt
Publisher : Humana
Page : 0 pages
File Size : 41,7 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493941615

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RNA-RNA Interactions by Frank J. Schmidt Pdf

In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.

Biophysics of RNA-Protein Interactions

Chirlmin Joo,David Rueda
Biophysics of RNA Protein Interactions Book PDF

Author : Chirlmin Joo,David Rueda
Publisher : Springer Nature
Page : 249 pages
File Size : 45,7 Mb
Release : 2019-09-19
Category : Science
ISBN : 9781493997268

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Biophysics of RNA-Protein Interactions by Chirlmin Joo,David Rueda Pdf

RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.

RNA Tagging

Manfred Heinlein
RNA Tagging Book PDF

Author : Manfred Heinlein
Publisher : Humana
Page : 0 pages
File Size : 53,9 Mb
Release : 2020-07-25
Category : Science
ISBN : 1071607111

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RNA Tagging by Manfred Heinlein Pdf

This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.

RNA-Chromatin Interactions

Ulf Andersson Vang Ørom
RNA Chromatin Interactions Book PDF

Author : Ulf Andersson Vang Ørom
Publisher : Humana
Page : 0 pages
File Size : 41,5 Mb
Release : 2020-07-18
Category : Medical
ISBN : 1071606794

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RNA-Chromatin Interactions by Ulf Andersson Vang Ørom Pdf

This volume focuses on RNAs interacting with chromatin and their function. Chapters guide readers through transcription, splicing, non-coding RNA function and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Chromatin Interactions: Methods and Protocols aims to be a starting-point to expand researchers experimental approaches towards the numerous outstanding questions in this new and expanding field.

Regulatory Non-Coding RNAs

Gordon G. Carmichael
Regulatory Non Coding RNAs Book PDF

Author : Gordon G. Carmichael
Publisher : Humana
Page : 0 pages
File Size : 44,7 Mb
Release : 2016-09-22
Category : Medical
ISBN : 1493941844

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Regulatory Non-Coding RNAs by Gordon G. Carmichael Pdf

Regulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips for troubleshooting and avoiding known pitfalls. Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.

RNA Abundance Analysis

Hailing Jin,Walter Gassmann
RNA Abundance Analysis Book PDF

Author : Hailing Jin,Walter Gassmann
Publisher : Humana Press
Page : 248 pages
File Size : 47,5 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493959166

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RNA Abundance Analysis by Hailing Jin,Walter Gassmann Pdf

This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.

Adenovirus Methods and Protocols

William S. M. Wold,Ann E. Tollefson
Adenovirus Methods and Protocols Book PDF

Author : William S. M. Wold,Ann E. Tollefson
Publisher : Springer Science & Business Media
Page : 738 pages
File Size : 40,5 Mb
Release : 2008-02-01
Category : Medical
ISBN : 9781597452779

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Adenovirus Methods and Protocols by William S. M. Wold,Ann E. Tollefson Pdf

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The protocols presented are written by trendsetting researchers.

DNA'Protein Interactions

Tom Moss
DNA Protein Interactions Book PDF

Author : Tom Moss
Publisher : Springer Science & Business Media
Page : 633 pages
File Size : 44,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592081

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DNA'Protein Interactions by Tom Moss Pdf

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

Two-Hybrid Systems

Luis Oñate-Sánchez
Two Hybrid Systems Book PDF

Author : Luis Oñate-Sánchez
Publisher : Humana
Page : 355 pages
File Size : 45,5 Mb
Release : 2019-06-15
Category : Science
ISBN : 1493993100

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Two-Hybrid Systems by Luis Oñate-Sánchez Pdf

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

Plant Long Non-Coding RNAs

Julia A. Chekanova,Hsiao-Lin V. Wang
Plant Long Non Coding RNAs Book PDF

Author : Julia A. Chekanova,Hsiao-Lin V. Wang
Publisher : Humana Press
Page : 480 pages
File Size : 42,5 Mb
Release : 2019-04-20
Category : Science
ISBN : 1493990446

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Plant Long Non-Coding RNAs by Julia A. Chekanova,Hsiao-Lin V. Wang Pdf

This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.

Protein-Nucleic Acid Interactions

Phoebe A. Rice,Carl C. Correll
Protein Nucleic Acid Interactions Book PDF

Author : Phoebe A. Rice,Carl C. Correll
Publisher : Royal Society of Chemistry
Page : 417 pages
File Size : 54,8 Mb
Release : 2008-05-22
Category : Science
ISBN : 9780854042722

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Protein-Nucleic Acid Interactions by Phoebe A. Rice,Carl C. Correll Pdf

This book provides both in-depth background and up-to-date information in this area. The chapters are organized by general themes and principles, written by experts who illustrate topics with current findings. Topics covered include: - the role of ions and hydration in protein-nucleic acid interactions - transcription factors and combinatorial specificity - indirect readout of DNA sequence - single-stranded nucleic acid binding proteins - nucleic acid junctions and proteins, - RNA protein recognition - recognition of DNA damage. It will be a key reference for both advanced students and established scientists wishing to broaden their horizons.